IFNA1 / Interferon-alpha 1 antibody

Principal name

IFNA1 / Interferon-alpha 1 antibody

Alternative names for IFNA1 / Interferon-alpha 1 antibody

IFNA13, Interferon alpha-D, LeIF D, IFN-alpha 1

SwissProt ID

P01562 (Human), P01572 (Mouse), P05003 (Horse), P05011 (Rat), P07348 (Bovin), P42165 (Chick), P49879 (Pig), P81255 (Canfa)

Gene ID

3439 (IFNA1), 298210 (Ifna1), 15962, 3447 (IFNA13), 396398 (IFNA3)

Available reactivities

Hu (Human), Por (Porcine), Bov (Bovine), Ms (Mouse), Rt (Rat), Chk (Chicken), Mky (Monkey), Can (Canine), Gt (Goat), Sh (Sheep)

Available hosts

Mouse, Rabbit, Rat

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Functional assay (FN), Immunoprecipitation (IP), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Neutralisation (NEUT), Flow Cytometry (F), Paraffin Sections (P)

Background of IFNA1 / Interferon-alpha 1 antibody

IFN-alpha comprises a family of structurally related proteins coded for by 14 non-allelic IFNalpha genes, one of which codes for IFN-alpha 2. Polymorphisms of the IFN-alpha 2 gene give rise to 3 isoforms, namely, IFN-alpha 2a (rarely expressed); IFN-alpha 2b (K=>R at amino acid 46 of the precursor); and IFN-alpha 2c (K=>R at amino acid 46, H=>R at amino acid 57). IFN-alpha is produced predominantly by monocytes/macrophages, and to a lesser extent by natural killer (NK) cells, T cells, dendritic cells and plasmacytoid dendritic cells (DCs). The expression of IFN-alpha is induced by viral or bacterial infections and also by the components of infectious agents such as LPS, bacterial DNA and double stranded RNA. IFNalpha treatment of DCs promotes the maturation and up-regulation of co-stimulatory molecules such as CD40, CD80, CD86 and MHC class II. This in turn facilitates antigen presentation, the priming of T cells and cytokine release to initiate immune responses. IFNalpha anti-tumor activity includes the capacity to promote apoptosis of tumor cells and inhibit angiogenesis by inducing the down regulation of basic fibroblast growth factor (FGF) and vascular endothelial growth factor (VEGF). The actions elicited by IFN-alpha result from binding to a common IFN cell surface receptor present on target cells, macrophages or DCs. Structurally, IFN-alpha 2 is a monomer and is O-glycosylated on Thr-129. This is a HCX protein. HCX Expression System Details HCX proteins mimic the proteins in the human body because they are expressed from human, rather than animal, insect or bacterial cells. This process gives them human post-translational modifications. Recombinant DNA techniques allow a human protein with the correct amino acid sequence to be expressed in a non-human cell line. However, non-human cells lack the appropriate cellular machinery, such as specific glycosyltransferases, necessary to produce the correct human post-translational modifications of a protein. An extreme example is seen in E. coli cells, which produce recombinant proteins with no glycosylation, as the above figure illustrates. Rodent and yeast cells are able to glycosylate proteins, but they are still different from glycosylation in human cells. Expression System Resultant Proteins Human (e.g. K562, HEK293) Correct amino acid sequence Human post-translational modifications Rodent (e.g. CHO, NSO) Correct amino acid sequence Some natural glycosylation - not human-like Yeast (e.g. Pichia) Correct amino acid sequence Some natural glycosylation - not human-like E.Coli Correct amino acid sequence No PTMs Although there have been significant attempts to make non-human cell derived cytokines more human-like, there is a growing awareness that in many instances, particularly in therapeutics, cytokines should mimic those found in the body as closely as possible.

General readings

Kontsek, P. et al. Immunodominant structures in the aminotermonal portion of human interferon alpha 1. Mol Immunol. 29: 863-870 (1992)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

IFNA1 / Interferon-alpha 1 antibody

WB Suggested Anti-IFNA13 Antibody; Titration: 1.0 ug/ml; Positive Control: Placenta Rabbit IgG Purified Bov, Can, Gt, Hu, Por, Sh WB
50 µg / €325.00
  OriGene Technologies, Inc.

IFNA1 / Interferon-alpha 1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IFNA1 (RC210902, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IFNA1. Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

IFNA1 / Interferon-alpha 1 antibody

IFNA1 / Interferon-alpha 1 Mouse IgG1 2-52 Purified Hu C, E, WB
0.1 mg / €300.00
  Acris Antibodies GmbH

IFNA1 / Interferon-alpha 1 antibody

IFNA1 / Interferon-alpha 1 Mouse IgG1 2-48 Purified Hu C, E, WB
0.1 mg / €300.00
  Acris Antibodies GmbH

IFNA1 / Interferon-alpha 1 antibody

IFNA1 / Interferon-alpha 1 Rabbit Serum Bov WB
0.1 ml / €380.00
  Acris Antibodies GmbH

IFNA1 / Interferon-alpha 1 antibody

IFNA1 / Interferon-alpha 1 Rabbit Serum Bov WB
20 µl / €150.00
  Acris Antibodies GmbH

IFNA1 / Interferon-alpha 1 antibody

IFNA1 / Interferon-alpha 1 Rat IgG1 F18 Purified Ms E, F, FN
0.1 mg / €300.00
  Acris Antibodies GmbH

IFNA1 / Interferon-alpha 1 antibody

IFNA1 / Interferon-alpha 1 Mouse IgG1 G16 Azide Free Por E, FN, IP
0.25 mg / €610.00
  Acris Antibodies GmbH

IFNA1 / Interferon-alpha 1 antibody

IFNA1 / Interferon-alpha 1 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

IFNA1 / Interferon-alpha 1 antibody

Western Blot analysis of IFNA1 expression in transfected 293T cell line (H00003439-T02) by IFNA1 MaxPab polyclonal antibody.

Lane 1: IFNA1 transfected lysate(21.70 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

IFNA1 / Interferon-alpha 1 antibody

IFNA1 / Interferon-alpha 1 Mouse Hu WB
50 µg / €370.00
  Abnova Taiwan Corp.

IFNA1 / Interferon-alpha 1 antibody

IFNA1 / Interferon-alpha 1 Rabbit Serum Hu E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

IFNA1 / Interferon-alpha 1 antibody

Western Blot analysis of IFNA1 expression in transfected 293T cell line (H00003439-T01) by IFNA1 MaxPab polyclonal antibody.

Lane 1: IFNA1 transfected lysate(21.70 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

IFNA1 / Interferon-alpha 1 antibody

IFNA1 / Interferon-alpha 1 Mouse IgG2b 3C6 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

IFNA1 / Interferon-alpha 1 antibody

IFNA1 / Interferon-alpha 1 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

IFNA1 / Interferon-alpha 1 antibody

Western Blot analysis of IFNA13 expression in transfected 293T cell line (H00003447-T01) by IFNA13 MaxPab polyclonal antibody.

Lane 1: IFNA13 transfected lysate(20.79 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

IFNA1 / Interferon-alpha 1 antibody

Western Blot analysis of IFNA13 expression in transfected 293T cell line (H00003447-T02) by IFNA13 MaxPab polyclonal antibody.

Lane 1: IFNA13 transfected lysate(21.70 KDa).
Lane 2: Non-transfected lysate. Rabbit Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

IFNA1 / Interferon-alpha 1 antibody

IFNA1 / Interferon-alpha 1 Mouse IgG1 3F9 Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

IFNA1 / Interferon-alpha 1 antibody

IFNA1 / Interferon-alpha 1 Rabbit Ms WB
0.1 ml / €470.00
  Abnova Taiwan Corp.

IFNA1 / Interferon-alpha 1 (C-term) antibody

Human Placenta; WB Suggested Anti-IFNA13 Antibody. . Titration: 1.0 ug/ml. . Positive Control: Placenta; IFNA13 antibody - C-terminal region (ARP60732_P050) in Human Placenta cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

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