IFNG / Interferon gamma antibody

Principal name

IFNG / Interferon gamma antibody

Alternative names for IFNG / Interferon gamma antibody

IFN-gamma, gamma IFN

SwissProt ID

O35497 (Mesau), P01579 (Human), P01580 (Mouse), P01581 (Rat), P07353 (Bovin), P17773 (Sheep), P17803 (Pig), P30123 (Rabit), P42160 (Horse), P42161 (Canfa), P46402 (Felca), P49708 (Chick)

Gene ID

3458 (IFNG), 25712 (Ifng), 15978, 281237, 396054, 493965, 396991

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey), Chk (Chicken), Bov (Bovine), Bab (Baboon), Sh (Sheep), Can (Canine), Eq (Equine), Gt (Goat), Por (Porcine), Md (Mustelid), Prim (Primate), Do (Dolphin)

Available hosts

Mouse, Rat, Rabbit, Goat, Hamster, Chicken, Sheep

Available applications

Enzyme Immunoassay (E), ELISA (capture) (E(capture)), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Frozen Sections (C), Functional assay (FN), Immunoprecipitation (IP), Paraffin Sections (P), Flow Cytometry (F), Neutralisation (NEUT), Immunocytochemistry/Immunofluorescence (ICC/IF), Radioimmunoassay (R), Luminex (LMNX)

Background of IFNG / Interferon gamma antibody

The Interferon gamma (IFN-gamma; 16-25 kDa) is an important regulator of the immune response, produced in activated Th1 cells and NK cells, particularly in response to IL-2, TNF-alpha and IL-12; its production is suppressed by IL-4, IL-10, and TGF-beta. The producing of IFN-gamma is activated by specific antigens or mitogens through the T cell antigen receptor. IFN-gamma polypeptide forms: 40-60 kDa forms are observable under non-denaturing conditions as dimers and trimers; 20 kDa and 25 kDa forms exist due to variable glycosylation. IFN-gamma belongs to the type II interferons, also called immune IFN.
IFN-gamma shows antiviral activity and has important immunoregulatory functions. It is a potent activator of macrophages and had antiproliferative effects on transformed cells. IFN-gamma plays an important role in regulating B cell differentiation by simultaneously stimulating class switch recombination to the IgG3 and IgG2a isotypes while represing class switch recombination to the IgE and IgG1 isotypes. It also appears to promote antigen presentation by B cells through its effects on MHC.
Binding of IFN-gamma to its receptor increases the expression of class I MHC on all somatic cells. It also enhances the expression of class II MHC on antigen-presenting cells. IFN-gamma is the major means by which T cells activate macrophages, increasing their ability to kill bacteria, parasites, and tumours. The activation of macrophages by IFN-gamma is essential for the elimination of bacteria that replicate within the phagosomes of macrophages (f.e. Mycobacteria and Listeria monocytogenes). IFN-gamma can potentiate the high antiviral and antitumor effects of the type I interferons (IFN-alpha, IFN-beta). IFN-gamma may also activate neutrophils and NK cells.
IFN-gamma shows antiviral activity and has important immunoregulatory functions. It is a potent activator of macrophages and had antiproliferative effects on transformed cells. IFN-gamma plays an important role in regulating B cell differentiation by simultaneously stimulating class switch recombination to the IgG3 and IgG2a isotypes while represing class switch recombination to the IgE and IgG1 isotypes. It also appears to promote antigen presentation by B cells through its effects on MHC. Binding of IFN-gamma to its receptor increases the expression of class I MHC on all somatic cells. It also enhances the expression of class II MHC on antigen-presenting cells. IFN-gamma is the major means by which T cells activate macrophages, increasing their ability to kill bacteria, parasites, and tumours. The activation of macrophages by IFN-gamma is essential for the elimination of bacteria that replicate within the phagosomes of macrophages (f.e. Mycobacteria and Listeria monocytogenes). IFN-gamma can potentiate the high antiviral and antitumor effects of the type I interferons (IFN-alpha, IFN-beta). IFN-gamma may also activate neutrophils and NK cells.

General readings

1. Paineau, J. et al. (1991) Effect of recombinant interferon-gamma and interleukin2
and of a monoclonal antibody against interferon gamma on the rat immune response against heart allografts. J. Heart Lung Transplant. 10: 424430.
2. Graf, M. et al. (2001) IL-6 secretion by a rat T9 Glioma clone induces a neutrophil-dependent antitumor response with resultant cellular, antiglioma immunity. J. Immunol. 166: 121-129.
3. Paineau, J. et al. (1989) Effects of gamma interferon and interleukin-2, and of gamma-interferon antibodies, on the rat immune response against allografts. Transplant Proc. 21: 9991001.
4. Van der Meide, P.H. et al. (1986) The purification and characterization of rat gamma interferon by use of two Monoclonal antibodies. J. Gen. Virol. 67: 10591071.
5. Keller, R. et al. (1987) Induction, maintenance, and reinduction of tumoricidal activity in bone marrow-derived mononuclear phagocytes by Corynebacterium parvum. Evidence for the involvement of a T celland interferon-gamma-independent pathway of macrophage activation. J. Immunol. 138: 236612371.
6. Jacob, C.O. et al. (1989) Heterogeneous effects of IFN-gamma in adjuvant arthritis. J. Immunol. 142: 15001505.
7. Kingston, A.E. et al. (1989) Schwann cells cocultured with stimulated T cells and antigen express major histocompatibility complex (MHC) class II determinants without interferon-gamma pretreatment: synergistic effects of interferon-gamma and tumor necrosis factor on MHC class II induction. Eur. J. Immunol. 19: 177183.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

IFNG / Interferon gamma antibody

Immunohistochemistry The Interferon gamma antibody stained formalin-fixed, paraffin-embedded sections of human cervical squamous cell carcinoma. The recommended concentration is 0.5 µg/ml-0.25µg/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. Rabbit Aff - Purified Hu E, FN, P, WB
50 µg / €230.00
  Acris Antibodies GmbH

IFNG / Interferon gamma antibody

Immunohistochemistry The Interferon gamma antibody stained formalin-fixed, paraffin-embedded sections of human cervical squamous cell carcinoma. The recommended concentration is 0.5 µg/ml-0.25µg/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. Rabbit Aff - Purified Hu E, FN, P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

IFNG / Interferon gamma antibody

To detect Human IFN-gamma; by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Human IFN-gamma;. Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

IFNG / Interferon gamma antibody

To detect Human IFN-gamma; by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IFN-gamma; (TA328343) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IFN-gamma;. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

IFNG / Interferon gamma antibody

To detect Murine IFN-gamma; by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Murine IFN-gamma;. Rabbit IgG Purified E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

IFNG / Interferon gamma antibody

To detect Murine IFN-gamma; by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine IFN-gamma; (TA328518) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Murine IFN-gamma;. Rabbit IgG Purified E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

IFNG / Interferon gamma antibody

To detect Rat IFN-gamma; by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat IFN-gamma;. Rabbit IgG Purified Rt E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

IFNG / Interferon gamma antibody

To detect Rat IFN-gamma; by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat IFN-gamma;. Goat IgG Purified Rt E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

IFNG / Interferon gamma antibody

To detect Rat IFN-gamma; by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Rat IFN-gamma; (TA328583) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IFN-gamma;. Goat IgG Purified Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

IFNG / Interferon gamma antibody

To detect Rat IFN-gamma; by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Rat IFN-gamma; (TA328582) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IFN-gamma;. Rabbit IgG Purified Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

IFNG / Interferon gamma antibody

Western blot analysis of mouse brain cell lysate using IFNG antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

IFNG / Interferon gamma antibody

Western blot analysis of Interferon gamma expression in HepG2 cells, Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

IFNG / Interferon gamma antibody

ELISA: Interferon gamma Antibody Rabbit IgG Purified Hu E
0.1 mg / €325.00
  OriGene Technologies, Inc.

IFNG / Interferon gamma antibody

lFN-g ELISA with B140 Capture (TA600016) and B308 Detection (TA700016) Antibodies. Substrate used: Recombinant Human lFN-g (E. Coli expressed Peptide) Mouse IgG1 OTIB140 Purified Hu E, LMNX
0.1 mg / €325.00
  OriGene Technologies, Inc.

IFNG / Interferon gamma antibody

lFN-g ELISA with B140 Capture (TA600016) and B308 Detection (TA700016) Antibodies. Substrate used: Recombinant Human lFN-g (E. Coli expressed Peptide) Mouse IgG1 OTIB308 Purified Hu E, LMNX
50 µg / €345.00
  OriGene Technologies, Inc.

IFNG / Interferon gamma antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IFNG (RC209993, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IFNG. Mouse IgG1 OTI1G7 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

IFNG / Interferon gamma antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IFNG (RC209993, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IFNG. Mouse IgG2b OTI1G10 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

IFNG / Interferon gamma antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IFNG (RC209993, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IFNG.(1:2000) Mouse IgG1 OTI4D7 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

IFNG / Interferon gamma antibody

IFNG / Interferon gamma Mouse IgG1 G-23 Purified Hu C, E, WB
0.2 mg / €400.00
  Acris Antibodies GmbH

IFNG / Interferon gamma antibody

IFNG / Interferon gamma Mouse IgG1 G-23 Aff - Purified Hu F, WB
0.1 mg / €210.00
  Acris Antibodies GmbH

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