IFNGR2 / IFNGT1 antibody
Principal name
IFNGR2 / IFNGT1 antibody
Alternative names for IFNGR2 / IFNGT1 antibody
IFN-gamma receptor 2, IFN-gamma-R2, Interferon-gamma receptor beta, AF1, Interferon-gamma receptor accessory factor 1, Interferon-gamma transducer 1
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Flow Cytometry (F), Paraffin Sections (P), Immunoprecipitation (IP), Frozen Sections (C), Neutralisation (NEUT)
Background of IFNGR2 / IFNGT1 antibody
IFN gamma receptor beta is part of the receptor for interferon gamma. This class II cytokine receptor pairs with CDw119 to form the IFN gamma receptor and is an integral part of the IFN gamma signal transduction pathway. CDw119 serves as the IFN gamma binding chain and associates with the IFN gamma beta chain which is required for receptor signaling. The extracellular portion of both the IFN gamma receptor alpha and beta chains must be species matched. The IFN gamma receptor beta chain is expressed on T and B cells, NK cells, monocytes/ macrophages, and fibroblasts. Binding of IFN gamma induces receptor dimerization, internalization, Jak1 and Jak2 protein kinase activation and, ultimately, STAT1 activation. It is also likely to interact with GAF. IFN gamma initiates and regulates a variety of immune responses and is required for signal transduction. Contains 2 fibronectin type III domains. Defects in IFN gamma Receptor beta are a cause of mendelian susceptibility to mycobacterial disease (MSMD), a rare condition that confers predisposition to illness caused by several mycobacteria strains.
General readings
Oritani et al., (2001) Cytokine Growth Factor Rev. 12(4): 337-48.
A549 cells were subjected to SDS PAGE followed by western blot with 10266-1-AP(IFNGR2 antibody) at dilution of 1:500
HeLa cells were subjected to SDS PAGE followed by western blot with 10266-1-AP(IFNGR2 antibody) at dilution of 1:500
HepG2 cells were subjected to SDS PAGE followed by western blot with 10266-1-AP(IFNGR2 antibody) at dilution of 1:500
Detection limit for recombinant GST tagged IFNGR2 is approximately 0.3ng/ml as a capture antibody.
Western Blot analysis of IFNGR2 expression in transfected 293T cell line (H00003460-T01) by IFNGR2 MaxPab polyclonal antibody.Lane 1: IFNGR2 transfected lysate(37.07 KDa).Lane 2: Non-transfected lysate.
FACS analysis of negative control 293 cells (Black) and IFNGR2 expressing 293 cells (Green) using IFNGR2 purified MaxPab mouse polyclonal antibody.
Western Blot analysis of IFNGR2 expression in transfected 293T cell line (H00003460-T02) by IFNGR2 MaxPab polyclonal antibody.Lane 1: IFNGR2 transfected lysate(37.80 KDa).Lane 2: Non-transfected lysate.
IFNGR2 MaxPab rabbit polyclonal antibody. Western Blot analysis of IFNGR2 expression in HeLa.
Western Blot: IFN gamma Receptor beta Lysate [NBL1-11853] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for IFNGR2
Human Liver; WB Suggested Anti-IFNGR2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human Liver; IFNGR2 antibody - middle region (ARP46611_P050) in Human Liver cells using Western Blot
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with IFNGR2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of IFNGR2 Antibody (C-term) (AP17492PU-N) in MDA-MB435 cell line lysates (35ug/lane). IFNGR2 (arrow) was detected using the purified Pab.
IFNGR2 MaxPab rabbit polyclonal antibody. Western Blot analysis of IFNGR2 expression in HeLa.
Immunoprecipitation of IFNGR2 transfected lysate using anti-IFNGR2 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with IFNGR2 MaxPab mouse polyclonal antibody (B01) (H00003460-B01).
Western Blot: IFN gamma Receptor beta Antibody [NBP1-59795] - This Anti-IFNGR2 antibody was used in Western Blot of Fetal Liver tissue lysate at a concentration of 1ug/ml.
Immunohistochemistry-Paraffin: IFN gamma Receptor beta Antibody [NBP1-90223] - Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.
Western Blot: IFN gamma Receptor beta Antibody [NBP1-90223] Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 | Lane 2: Negative control (vector only transfected HEK293T lysate) | Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells.
Flow cytometric analysis of NCI-H292 cells using IFNGR2 Antibody (C-term)(bottom histogram)(GTX81601) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of IFNGR2 Antibody (C-term) (GTX81601) in MDA-MB453 cell line lysates (35ug/lane). IFNGR2 (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with IFNGR2 Antibody (C-term)(GTX81601), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin embedded human labeled with Anti-IFNGR2 Polyclonal Antibody, Unconjugated (bs-2710R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-IFNGR2 mouse monoclonal antibody (TA506734) at 1:150 dilution.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IFNGR2 (RC200674, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IFNGR2.
Splenocytes from mice pre-immunized with PLP139-151 peptide were recall activated 4 weeks later with PLP139-151 for 24 hours in Mouse IFN gamma ELISPOT assay. Control well is medium alone.
WB Suggested Anti-IFNGR2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Human Liver
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA320354 | IFNGR2 / IFNGT1 antibody |
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Mouse | 4S.B3 | Purified | Hu | F |
50 µg /
€120.00
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| OriGene Technologies, Inc. | |||||||
| TA320355 | IFNGR2 / IFNGT1 antibody |
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 |
Rat | AN-18 | Purified | Ms | E, IP |
50 µg /
€120.00
|
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| OriGene Technologies, Inc. | |||||||
| TA320356 | IFNGR2 / IFNGT1 antibody |
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Mouse | NIB42 | Purified | Hu | E |
50 µg /
€120.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA320357 | IFNGR2 / IFNGT1 antibody |
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|
Rat | R4-6A2 | Purified | Ms |
0.5 mg /
€248.00
|
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| OriGene Technologies, Inc. | |||||||
| TA339011 | IFNGR2 / IFNGT1 antibody |
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 |
Rabbit | IgG | Purified | Hu | WB |
50 µg /
€325.00
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| OriGene Technologies, Inc. | |||||||
| TA506734 | IFNGR2 / IFNGT1 antibody |
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 |
Mouse | IgG2b | OTI1C2 | Purified | Hu | P, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
|
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| AP17492PU-N | IFNGR2 / IFNGT1 (C-term) antibody |
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 |
Rabbit | Hu | P, WB |
0.4 ml /
€370.00
|
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| Acris Antibodies GmbH | |||||||
| +1 additional image |
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| H00003460-B01P | IFNGR2 / IFNGT1 antibody |
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Mouse | Purified | Hu | E(detection), F, WB |
50 µg /
€370.00
|
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| Abnova Taiwan Corp. | |||||||
| +1 additional image |
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| H00003460-D01 | IFNGR2 / IFNGT1 antibody |
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Rabbit | Serum | Hu | E(detection), IP, WB |
0.1 ml /
€380.00
|
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| Abnova Taiwan Corp. | |||||||
| +1 additional image |
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| H00003460-D01P | IFNGR2 / IFNGT1 antibody |
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 |
Rabbit | Purified | Hu | E(detection), WB |
0.1 mg /
€380.00
|
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| Abnova Taiwan Corp. | |||||||
| +1 additional image |
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| H00003460-M01 | IFNGR2 / IFNGT1 antibody |
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Mouse | IgG2a | 2D9 | Purified | Hu | E |
0.1 mg /
€370.00
|
| Abnova Taiwan Corp. | |||||||
| ARP46611_P050 | IFNGR2 / IFNGT1 antibody |
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Rabbit | Aff - Purified | Hu | WB |
50 µg /
€350.00
|
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| AVIVA Systems Biology | |||||||
| NBP1-59795 | IFNGR2 / IFNGT1 antibody |
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![Western Blot: IFN gamma Receptor beta Antibody [NBP1-59795] - This Anti-IFNGR2 antibody was used in Western Blot of Fetal Liver tissue lysate at a concentration of 1ug/ml.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/IFN gamma Receptor beta Antibody-Western Blot-NBP1-59795-img0001.jpg) |
Rabbit | Aff - Purified | Hu | WB |
50 µg /
€440.00
|
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| Novus Biologicals Inc. | |||||||
| NBP1-90223 | IFNGR2 / IFNGT1 antibody |
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![Immunohistochemistry-Paraffin: IFN gamma Receptor beta Antibody [NBP1-90223] - Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/IFN gamma Receptor beta Antibody-Immunohistochemistry-Paraffin-NBP1-90223-0005.jpg) |
Rabbit | IgG | Aff - Purified | Hu | P, WB |
0.1 ml /
€500.00
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| Novus Biologicals Inc. | |||||||
| +1 additional image |
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| PBL21585-1 | IFNGR2 / IFNGT1 (Non-Neutralizing) antibody |
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Mouse | IgG1 | MMHGR-2 | Purified | Hu | E |
0.25 mg /
€250.00
|
| PBL Assay Science | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP300674 | IFNGR2 / IFNGT1 |
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
Regular Price: 20 µg / €680.00 Special Price: 20 µg / €398.00 |
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| OriGene Technologies, Inc. | |||||
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| H00003460-P01 | IFNGR2 / IFNGT1 |
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Human |
2 µg /
€300.00
|
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| Abnova Taiwan Corp. | |||||
| H00003460-Q01-10 | IFNGR2 / IFNGT1 |
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Human | Purified | 12.5% SDS-PAGE Stained with Coomassie Blue. |
10 µg /
€380.00
|
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| Abnova Taiwan Corp. | |||||
| H00003460-Q01-25 | IFNGR2 / IFNGT1 |
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Human | Purified | 12.5% SDS-PAGE Stained with Coomassie Blue. |
25 µg /
€510.00
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| Abnova Taiwan Corp. | |||||
| NBP1-46064-25-25 | IFNGR2 / IFNGT1 |
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Human | Purified |
25 µg /
€290.00
|
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| Novus Biologicals Inc. | |||||
