IFT74 antibody

Principal name

IFT74 antibody

Alternative names for IFT74 antibody

CCDC2, CMG1, CMG-1, Intraflagellar transport protein 74 homolog, Coiled-coil domain-containing protein 2, Capillary morphogenesis protein 1

SwissProt ID

Q8BKE9 (Mouse), Q96LB3 (Human)

Gene ID

80173 (IFT74)

Available reactivities

Hu (Human), Rt (Rat), Ms (Mouse), Rb (Rabbit), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Ye (Yeast)

Available hosts

Goat, Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Paraffin Sections (P), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Dot blot (Dot), Frozen Sections (C)

Background of IFT74 antibody

Capillary morphogenesis protein 1 (CMG1) is a 600 amino acid protein and has a calculated molecular mass of 65 kD. CMG1 contains a coiled-coil domain (which shares 24% identity with myosin heavy chain sequences from various species) and several putative phosphorylation sites. Bell et al. (2001) determined that CMG1 was 1 of several transcripts upregulated by umbilical vein endothelial cells during capillary morphogenesis in 3-dimensional collagen matrices. RT-PCR detected highest expression in adult and fetal kidney and lower expression in adult heart, placenta, lung, liver, and pancreas, and in fetal heart, lung, and liver. Little to no expression was detected in adult brain and skeletal muscle or in fetal brain, thymus, and spleen. Transfection into epithelial tumor cells revealed targeting of fluorescent-tagged CMG1 to an intracellular vesicular compartment.

General readings

Bell SE, Mavila A, Salazar R, Bayless KJ, Kanagala S, Maxwell SA, Davis GE. Differential gene expression during capillary morphogenesis in 3D collagen matrices: regulated expression of genes involved in basement membrane matrix assembly, cell cycle progression, cellular differentiation and G-protein signaling.
J Cell Sci. 2001 Aug;114(Pt 15):2755-73.
PMID: 11683410

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

IFT74 (588-600) antibody

Liver: Formalin-Fixed Paraffin-Embedded (FFPE) Goat IgG Aff - Purified Hu, Mky, Prim, Rb E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

IFT74 (C-term) antibody

TA302937 staining (0.3µg/ml) of 293 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat Aff - Purified Hu, Rt E, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

IFT74 antibody

Sample(30 ug of whole cell lysate). A: Raji. 12% SDS PAGE. TA308304 diluted at 1:500 Rabbit IgG Aff - Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

IFT74 antibody

Host: Rabbit; Target Name: Ift74; Sample Tissue: Rat Lung lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ye WB
50 µg / €325.00
  OriGene Technologies, Inc.

IFT74 antibody

IFT74 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

IFT74 antibody

IFT74 Mouse IgG1 2B6 Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

IFT74 antibody

A ) IFT74 polyclonal antibody ( Cat # PAB6611 ) ( 2.5 mg/ml ) staining of primary rat cortical neurons showed localization of IFT74 to vesicles in the cell body and along the neuronal processes. B ) Control. ( Data were kindly provided by Dr. Bryan Traynor. See Application Notes! ) Goat Hu, Rt E, ICC/IF, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

IFT74 (C-term) antibody

Human MCF-7; WB Suggested Anti-IFT74 Antibody. . Titration: 1.0 ug/ml. . Positive Control: MCF7 Whole Cell; IFT74 antibody - C-terminal region (ARP62026_P050) in Human MCF-7 cells using Western Blot Rabbit Aff - Purified Hu
50 µg / €350.00
  AVIVA Systems Biology

IFT74 antibody

Figure 1: NB300-822 (5ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining Goat IgG Aff - Purified Hu, Rt E, ICC/IF, P, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

IFT74 antibody

Western Blot: IFT74 Antibody [NBP1-31097] - Western blot analysis of IFT74 Antibody on: A:Hep G2 B:Raji 12% SDS PAGE diluted at 1:500 Rabbit IgG Hu Dot, ICC/IF, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

IFT74 antibody

Immunohistochemistry-Paraffin: IFT74 Antibody [NBP1-86580] - Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in cells in tubules. Rabbit IgG Aff - Purified Hu ICC/IF, P
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

IFT74

IFT74 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

IFT74

IFT74 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

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