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IFT74 antibody

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Principal name

IFT74 antibody

Alternative names for IFT74 antibody

CCDC2, CMG1, CMG-1, Intraflagellar transport protein 74 homolog, Coiled-coil domain-containing protein 2, Capillary morphogenesis protein 1

SwissProt ID

Q8BKE9 (Mouse), Q96LB3 (Human)

Gene ID

80173 (IFT74), 67694 (Ift74)

Ncbi ID

9606, NP_001092692.1, NM_025103, NP_079379, NP_080595.2, BC049156, NM_001099222, NM_001099224

Available reactivities

Hu (Human), Rt (Rat), Mky (Monkey), Prim (Primate), Rb (Rabbit), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Ms (Mouse), Por (Porcine), Ye (Yeast)

Available hosts

Mouse, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of IFT74 antibody

Capillary morphogenesis protein 1 (CMG1) is a 600 amino acid protein and has a calculated molecular mass of 65 kD. CMG1 contains a coiled-coil domain (which shares 24% identity with myosin heavy chain sequences from various species) and several putative phosphorylation sites. Bell et al. (2001) determined that CMG1 was 1 of several transcripts upregulated by umbilical vein endothelial cells during capillary morphogenesis in 3-dimensional collagen matrices. RT-PCR detected highest expression in adult and fetal kidney and lower expression in adult heart, placenta, lung, liver, and pancreas, and in fetal heart, lung, and liver. Little to no expression was detected in adult brain and skeletal muscle or in fetal brain, thymus, and spleen. Transfection into epithelial tumor cells revealed targeting of fluorescent-tagged CMG1 to an intracellular vesicular compartment.

General readings

1. Momeni P et al.: Analysis of IFT74 as a candidate gene for chromosome 9p-linked ALS-FTD.
BMC Neurol. 2006 Dec 13;6(1):44. PMID: 17166276
2.Boldt K et.al. Disruption of intraflagellar protein transport in photoreceptor cilia causes Leber congenital amaurosis in humans and mice. J Clin Invest. 2011 Jun 1;121(6):2169-80. PMID: 21606596

PAB6611 - IFT74
A ) IFT74 polyclonal antibody ( Cat # PAB6611 ) ( 2.5 mg/ml ) staining of primary rat cortical neurons showed localization of IFT74 to vesicles in the cell body and along the neuronal processes. B ) Control. ( Data were kindly provided by Dr. Bryan Traynor. See Application Notes! )
PAB6611 - IFT74
IFT74 polyclonal antibody ( Cat # PAB6611 ) staining ( 0.3 µg/ml ) of 293 lysate ( RIPA buffer, 35 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
AP08569PU-N - IFT74
Liver: Formalin-Fixed Paraffin-Embedded (FFPE)
AP08569PU-N - IFT74
Antibody staining (0.3 µg/ml) of 293 lysate (RIPA buffer, 35 µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using Chemiluminescence.
ARP62026_P050 - IFT74
Human MCF-7; WB Suggested Anti-IFT74 Antibody. . Titration: 1.0 ug/ml. . Positive Control: MCF7 Whole Cell; IFT74 antibody - C-terminal region (ARP62026_P050) in Human MCF-7 cells using Western Blot
TA302937 - IFT74
TA302937 staining (0.3µg/ml) of 293 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
TA308304 - IFT74
Sample(30 ug of whole cell lysate). A: Raji. 12% SDS PAGE. TA308304 diluted at 1:500
TA308304 - IFT74
Immunofluorescence analysis of methanol-fixed A431, using CMG1(TA308304) antibody at 1:500 dilution.
TA343188 - IFT74
Host: Rabbit; Target Name: Ift74; Sample Tissue: Rat Lung lysates; Antibody Dilution: 1.0 ug/ml
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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

IFT74 (588-600) antibody
Liver: Formalin-Fixed Paraffin-Embedded (FFPE) Goat IgG Aff - Purified Hu, Mky E, P, WB
50 µg / €425.00
  OriGene Technologies GmbH
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Product Type
  1. Primary Antibody (1)
Clonality
  1. Polyclonal (1)
Property
  1. 588-600 (1)
Applications
  1. Enzyme Immunoassay (E) (1)
  2. Paraffin Sections (P) (1)
  3. Western blot / Immunoblot (WB) (1)
Presentation
  1. Aff - Purified (1)
Host
  1. Goat (1)
Images
  1. 1 image (1)
Images: Applications
  1. Paraffin Sections (P) (1)
  2. Western blot / Immunoblot (WB) (1)
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