IGFBP3 antibody

Principal name

IGFBP3 antibody

Alternative names for IGFBP3 antibody

IGFBP-3, IGF-BP3, IBP3, Insulin-like growth factor-binding protein 3, IGF-binding protein 3, IBP-3

SwissProt ID

P15473 (Rat), P16611 (Pig), P17936 (Human), P20959 (Bovin), P47878 (Mouse)

Gene ID

3486 (IGFBP3), 24484 (Igfbp3), 3334860 (accD)

Available reactivities

Hu (Human), Ms (Mouse), Can (Canine), Rt (Rat)

Available hosts

Mouse, Rabbit, Goat, Rat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Frozen Sections (C)

Background of IGFBP3 antibody

IGFBP-3 is a member of the insulin-like growth factor binding protein (IGFBP) family. This family includes IGFBP-1 to IGFBP-6 that bind to the insulin-like growth factors (IGFs), thus regulating the half-life, activity, transport, and tissue distribution of the IGFs. Structurally, the IGFBP family of proteins is defined by highly homologous amino and carboxy terminal domains with a structurally diverse central region. The IGF binding motifs exist in the amino and carboxy terminal regions. The carboxy terminal domains may also be involved in interactions with numerous other molecules that may modulate IGF-binding as well as conferring IGF-independent function. IGFBP-3 is the most abundant IGFBP found in human serum where it is predominantly found as a ternary complex with IGF-I or IGF-II and the acidlabile subunit (ALS). This complex accounts for the majority of circulating IGF. Functionally, IGFBP-3 has been observed to exhibit both cell growth promoting and inhibiting effects depending on its effect on the bioavailability of IGF, and its IGF-independent function. IGFBP-3 is a 264 amino acid glycoprotein. In addition to glycosylation IGFBP-3 can also be phosphorylated and these post-translational modifications influence functional activity of IGFBP-3 by modulating tissue targeting, cell interaction and susceptibility to proteolytic cleavage. IGFBP-3 contains three potential N-linked glycosylation sites (Asn-X-Ser/Thr) located in the central linker domain at Asn 89 Asn 109, and Asn 172, while phosphorylation occurs at Ser 111 and 113. This is a HCX protein. HCX Expression System Details HCX proteins mimic the proteins in the human body because they are expressed from human, rather than animal, insect or bacterial cells. This process gives them human post-translational modifications. Recombinant DNA techniques allow a human protein with the correct amino acid sequence to be expressed in a non-human cell line. However, non-human cells lack the appropriate cellular machinery, such as specific glycosyltransferases, necessary to produce the correct human post-translational modifications of a protein. An extreme example is seen in E. coli cells, which produce recombinant proteins with no glycosylation, as the above figure illustrates. Rodent and yeast cells are able to glycosylate proteins, but they are still different from glycosylation in human cells. Expression System Resultant Proteins Human (e.g. K562, HEK293) Correct amino acid sequence Human post-translational modifications Rodent (e.g. CHO, NSO) Correct amino acid sequence Some natural glycosylation - not human-like Yeast (e.g. Pichia) Correct amino acid sequence Some natural glycosylation - not human-like E.Coli Correct amino acid sequence No PTMs Although there have been significant attempts to make non-human cell derived cytokines more human-like, there is a growing awareness that in many instances, particularly in therapeutics, cytokines should mimic those found in the body as closely as possible.

General readings

Lin J, Manson JE, Lee IM, Cook NR, Buring JE, Zhang SM.
Intakes of calcium and vitamin D and breast cancer risk in women.
Arch Intern Med. 2007 May 28;167(10):1050-9.
PMID: 17533208

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

IGFBP3 antibody

TA303275 (0.02µg/ml) staining of Human Breast cancer lysate (35µg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide.  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

IGFBP3 antibody

Predicted band size: 24-41 kDa . Positive control: HepG2, 3T3, Hela, 293, Jurkat, K562, Raji cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lane1,2,3,4,5,6,7: HepG2, 3T3, Hela, 293, Jurkat, K562, Raji cells lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

IGFBP3 antibody

Western blot analysis of IGFBP-3 expression in HuvEc whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

IGFBP3 antibody

This antibody stained formalin-fixed, paraffin-embedded sections of normal human placenta.  The recommended concentrations are 2.5ug/ml-0.5 ug/ml for two hours at room temperature.  An HRP-labeled polymer detection system was used with DAB chromogen.  Heat induced antigen retrieval was performed with a pH 6.0 Sodium Citrate buffer.  Optimal concentrations and conditions may vary. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

IGFBP3 antibody

ELISA: IGFBP3 Antibody Rabbit IgG Purified Hu E
0.1 mg / €325.00
  OriGene Technologies, Inc.

IGFBP3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IGFBP3 (RC216898, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IGFBP3. Mouse IgG2b OTI3B9 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

IGFBP3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IGFBP3 (RC216898, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IGFBP3. Mouse IgG2b OTI8E11 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

IGFBP3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IGFBP3 (RC216898, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IGFBP3.(1:2000) Mouse IgG1 OTI16B1 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

IGFBP3 antibody

IGFBP3 Mouse IgG1 83.8F9 Purified Hu E
0.5 mg / €650.00
  Acris Antibodies GmbH

IGFBP3 antibody

IGFBP3 Rabbit Biotin Hu E, WB
50 µg / €340.00
  Acris Antibodies GmbH

IGFBP3 antibody

IGFBP3 Rabbit Biotin Hu E, WB
25 µg / €230.00
  Acris Antibodies GmbH

IGFBP3 antibody

IGFBP3 Rabbit Aff - Purified Hu E, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

IGFBP3 antibody

IGFBP3 Rabbit Aff - Purified Hu E, WB
50 µg / €230.00
  Acris Antibodies GmbH

IGFBP3 (N-term) antibody

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with AP14345PU-N IGFBP3 Antibody (N-term) which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P, WB

Regular Price: 0.4 ml / €370.00

Special Price: 0.4 ml / €231.00

  Acris Antibodies GmbH

IGFBP3 antibody

Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with IGFBP3 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

IGFBP3 antibody

Western blot (WB) analysis of IGFBP3 antibody (Cat.-No.: AP20503PU-N) in extracts from HUVEC cells. Rabbit Aff - Purified Hu, Ms P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

IGFBP3 pSer183 antibody

Western blot analysis of extracts from Rat brain tissue using IGFBP-3 (Phospho-Ser183) antibody AP55946PU-N.The lane on the right is treated with the antigen-specific peptide. Rabbit Aff - Purified Hu, Ms WB
0.1 ml / €340.00
  Acris Antibodies GmbH

IGFBP3 pSer183 antibody

Western blot analysis of extracts from Rat brain tissue using IGFBP-3 (Phospho-Ser183) antibody AP55946PU-N.The lane on the right is treated with the antigen-specific peptide. Rabbit Aff - Purified Hu, Ms WB
50 µl / €270.00
  Acris Antibodies GmbH

IGFBP3 antibody

IGFBP3 Rat IgG2 11B16 Purified Ms WB
0.1 mg / €440.00
  Acris Antibodies GmbH

IGFBP3 antibody

Western Blot analysis of IGFBP3 expression in transfected 293T cell line (H00003486-T01) by IGFBP3 MaxPab polyclonal antibody.

Lane 1: IGFBP3 transfected lysate(31.70 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

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