IKBKB / IKKB antibody

Principal name

IKBKB / IKKB antibody

Alternative names for IKBKB / IKKB antibody

I-kappa-B-kinase beta, IKK-beta, IKK-B, I-kappa-B kinase 2, IKK2, NFKBIKB, Inhibitor of nuclear factor kappa-B kinase subunit beta, Nuclear factor NF-kappa-B inhibitor kinase beta

SwissProt ID

O14920 (Human), O88351 (Mouse), Q95KV0 (Bovin), Q9QY78 (Rat)

Gene ID

3551 (IKBKB), 16150 (Ikbkb)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Por (Porcine), Chk (Chicken)

Available hosts

Mouse, Rabbit, Chicken

Available applications

Western blot / Immunoblot (WB), Immunoprecipitation (IP), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), ELISA (detection) (E(detection)), Flow Cytometry (F), PLA/Proximity ligation assay (PLA), Frozen Sections (C), Dot blot (Dot)

Background of IKBKB / IKKB antibody

Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. In addition to the NF-kappa-B inhibitors, phosphorylates several other components of the signaling pathway including NEMO/IKBKG, NF-kappa-B subunits RELA and NFKB1, as well as IKK-related kinases TBK1 and IKBKE. IKK-related kinase phosphorylations may prevent the overproduction of inflammatory mediators since they exert a negative regulation on canonical IKKs. Also phosphorylates other substrates including NCOA3, BCL10 and IRS1. Within the nucleus, acts as an adapter protein for NFKBIA degradation in UV-induced NF-kappa-B activation.

General readings

1. Fang L  et al. ATM regulates NF-?B-dependent immediate-early genes via RelA Ser 276 phosphorylation coupled to CDK9 promoter recruitment. Nucleic Acids Res N/A:N/A (2014). PubMed: 24957606
2. Wei W  et al. The breast cancer susceptibility gene product fibroblast growth factor receptor 2 serves as a scaffold for regulation of NF-?B signaling. Mol Cell Biol 32:4662-73 (2012). WB . PubMed: 22988296

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

IKBKB / IKKB antibody

IKK beta antibody detects IKBKB protein by Western blot analysis. A. 30 ug PC-12 whole cell lysate/extract. 7.5 % SDS-PAGE. IKK beta antibody (TA308825) dilution: 1:1000 Rabbit IgG Aff - Purified Hu, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

IKBKB / IKKB antibody

Human brain; WB Suggested Anti-IKBKB Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human brain; IKBKB antibody - N-terminal region (ARP32665_P050) in Human brain cells using Western Blot Rabbit Aff - Purified Bov, Can, Chk, Hu, Ms, Por, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

IKBKB / IKKB antibody

Immunohistochemistry-Paraffin: IKK beta Antibody (EPR6043) [NBP1-95829]  mouse kidney tissue using anti-IKK beta (IKBKB). Rabbit EPR6043 Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

IKBKB / IKKB antibody

Western blot analysis of IKK beta in Jurkat whole cell lysate with IKK beta antibody (C3) at 1:500 dilution. Rabbit IgG Aff - Purified Hu E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

IKBKB / IKKB (C-term) antibody

WB of anti-IKKß antibody reacted with HeLa cell extract. All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a 1:500 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5' each. The WB was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2,000 for 1h. Rabbit Serum Hu, Ms, Rt E, IP, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

IKBKB / IKKB antibody

Western blot analysis of IKK-beta expression in LPS treated 293 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

IKBKB / IKKB antibody

Western blot analysis of extracts of various cell lines, using IKBKB antibody. Rabbit IgG Purified Hu, Ms
0.1 mg / €325.00
  OriGene Technologies, Inc.

IKBKB / IKKB antibody

IKBKB / IKKB Rabbit IgG Purified Hu, Ms, Rt P
0.1 ml / €325.00
  OriGene Technologies, Inc.

IKBKB / IKKB antibody

Western Blot: IKK beta Antibody (10AG2) [TA336374] - Western blot analysis of A) human Daudi, B) HeLa, and C) mouse NIH3T3 lysate probed with IKKbeta antibody at 2 ug/ml. Mouse IgG1 10AG2 Purified Hu, Ms C, F, IP, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

IKBKB / IKKB antibody

Western Blot: IKK beta Antibody (10A9B6) [TA336378] - analysis of IKK beta in Daudi cell lysate using IKK beta antibody at 5 ug/ml. Mouse IgG1 10A9B6 Purified Hu, Ms F, IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

IKBKB / IKKB antibody

Western blot detection of IKKß in THP-1 and PC12 cell lysates using IKKß mouse mAb (1:500 diluted).Predicted band size:87KDa.Observed band size:87KDa. Mouse IgG1 1C3-B11-B12 Purified WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

IKBKB / IKKB antibody

Western blot analysis using IKBKB antibody Cat.-No AM06154SU-N against truncated IKBKB recombinant protein (Lane 1) and K562 cell lysate (Lane 2). Mouse IgG1 10A2 Ascites Hu E, WB
0.1 ml / €440.00
  Acris Antibodies GmbH

IKBKB / IKKB antibody

Immunohistochemical analysis of paraffin-embedded human colon carcinoma(A), breast carcinoma(B), kidney cell carcinoma (C), bladder carcinoma tumor (D), showing membrane and cytoplasmic localization using IKBKB antibody Cat.-No AM06155SU-N with DAB staining. Mouse IgG1 10A2C5B3 Ascites Hu P, WB
0.1 ml / €440.00
  Acris Antibodies GmbH

IKBKB / IKKB antibody

Figure 1. Western blot using anti-IKKbeta Mab (Cat. #AM11078PU-N) to detect IKKbeta in Jurkat cell lysate. Mouse IgG1 62AT216 Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

IKBKB / IKKB antibody

Immunohistochemistry (IHC) analyzes of IKK-β antibody (Cat.-No.: AP06396PU-N) in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

IKBKB / IKKB antibody

Immunohistochemistry (IHC) analysis of IKK-β antibody (Cat.-No.: AP06526PU-N) in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

IKBKB / IKKB antibody

Pancreas: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

IKBKB / IKKB (C-term) antibody

Figure 1. Staining IKK Beta (IKK2) in Placenta by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue. Rabbit Ig Fraction Hu, Ms, Rt E, ICC/IF, IP, P, WB
50 µl / €470.00
  Acris Antibodies GmbH

IKBKB / IKKB (310-376) antibody

Thymus: Formalin-Fixed Paraffin-Embedded (FFPE) Chicken IgY Azide Free Hu, Ms, Rt P, WB
50 µg / €470.00
  Acris Antibodies GmbH

IKBKB / IKKB antibody

Immunofluorescence staining of methanol-fixed HeLa cells using IKK-β Antibody Rabbit Aff - Purified Hu, Ms, Rt ICC/IF
0.1 ml / €270.00
  Acris Antibodies GmbH

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