IKBKE / IKKE antibody

Principal name

IKBKE / IKKE antibody

Alternative names for IKBKE / IKKE antibody

I kappa-B kinase epsilon, IKK-epsilon, IKK-E, IKKI, KIAA0151, Inducible I kappa-B kinase, IKK-I, Inhibitor of nuclear factor kappa-B kinase subunit epsilon

SwissProt ID

Q14164 (Human), Q9R0T8 (Mouse)

Gene ID

9641 (IKBKE), 5799641 (aaeA)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), Flow Cytometry (F)

Background of IKBKE / IKKE antibody

Serine/threonine kinase that plays an essential role in regulating inflammatory responses to viral infection, through the activation of the type I IFN, NF-kappa-B and STAT signaling. Also involved in TNFA and inflammatory cytokines, like Interleukin-1, signaling. Following activation of viral RNA sensors, such as RIG-I-like receptors, associates with DDX3X and phosphorylates interferon regulatory factors (IRFs), IRF3 and IRF7, as well as DDX3X. This activity allows subsequent homodimerization and nuclear translocation of the IRF3 leading to transcriptional activation of pro-inflammatory and antiviral genes including IFNB. In order to establish such an antiviral state, IKBKE forms several different complexes whose composition depends on the type of cell and cellular stimuli. Thus, several scaffolding molecules including IPS1/MAVS, TANK, AZI2/NAP1 or TBKBP1/SINTBAD can be recruited to the IKBKE-containing-complexes. Activated by polyubiquitination in response to TNFA and interleukin-1, regulates the NF-kappa-B signaling pathway through, at least, the phosphorylation of CYLD. Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. In addition, is also required for the induction of a subset of ISGs which displays antiviral activity, may be through the phosphorylation of STAT1 at 'Ser-708'. Phosphorylation of STAT1 at 'Ser-708' seems also to promote the assembly and DNA binding of ISGF3 (STAT1:STAT2:IRF9) complexes compared to GAF (STAT1:STAT1) complexes, in this way regulating the balance between type I and type II IFN responses. Protects cells against DNA damage-induced cell death. Also plays an important role in energy balance regulation by sustaining a state of chronic, low-grade inflammation in obesity, wich leads to a negative impact on insulin sensitivity. Phosphorylates AKT1.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

IKBKE / IKKE antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-IKBKE mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807632)(1:150) Mouse IgG1 OTI3C5 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

IKBKE / IKKE antibody

Western blot analysis of IKK epsilon in Jurkat whole cell lysate with IKK epsilon/IKK-i antibody at 1 ug/ml. Rabbit IgG Purified Hu ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

IKBKE / IKKE antibody

Western blot analysis of extracts of various cell lines, using IKBKE antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

IKBKE / IKKE antibody

Western Blot: IKBKE Antibody (72B587) [TA336385] - analysis of IKK epsilon in Jurkat lysate using IKK epsilon antibody at 2 ug/ml. I goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test. Mouse IgG2a 72B587 Purified Hu, Ms, Rt F, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

IKBKE / IKKE antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IKBKE (RC212481, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IKBKE.(1:2000) Mouse IgG2a OTI4F9 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

IKBKE / IKKE antibody

Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-IKBKE mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 oC for 3 min, TA807631)(1:150) Mouse IgG1 OTI1E4 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

IKBKE / IKKE antibody

Western blot analysis using IKBKE antibody Cat.-No AM06206SU-N against truncated IKBKE recombinant protein (Lane 1) and full-length IKBKE (aa1-716)-hIgGFc transfected COS7 cell lysate (Lane 2). Mouse IgG1 6B4B5 Ascites Hu WB
0.1 ml / €440.00
  Acris Antibodies GmbH

IKBKE / IKKE (701-716) antibody

Spleen: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Elephant, Hu, Mky, Por, Prim P, WB
50 µg / €470.00
  Acris Antibodies GmbH

IKBKE / IKKE (C-term) antibody

(LEFT)Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.  (RIGHT)Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with IKKE Antibody (C-term) which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

IKBKE / IKKE pThr501 antibody

IKBKE / IKKE Rabbit Aff - Purified Hu E, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

IKBKE / IKKE antibody

IKBKE / IKKE Mouse IgG1 2F1 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

IKBKE / IKKE antibody

IKBKE / IKKE Mouse IgG2b 2B6 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

IKBKE / IKKE antibody

Immunoperoxidase of monoclonal antibody to IKBKE on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml] Mouse IgG2b 1F7 Purified Hu E, P
0.1 mg / €370.00
  Abnova Taiwan Corp.

IKBKE / IKKE antibody

IKBKE / IKKE Mouse IgG2b 3D11 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

IKBKE / IKKE antibody

Western blot analysis of IKBKE. Using IKBKE monoclonal antibody, clone 72B587 ( Cat # MAB0081 ) at 2 µg/ml on 15 µgs of Jurkat cell lysate. Mouse IgG2a 72B587 Hu, Ms, Rt F, WB
0.1 mg / €470.00
  Abnova Taiwan Corp.

IKBKE / IKKE antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with IKBKE polyclonal antibody ( Cat # PAB3999 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Hu E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

IKBKE / IKKE pThr501 antibody

IKBKE / IKKE Rabbit Hu, Ms, Rt E, WB
0.1 mg / €560.00
  Abnova Taiwan Corp.

IKBKE / IKKE antibody

Immunofluorescence: IKBKE Antibody [NBP1-33052] - Immunofluorescence analysis of methanol-fixed HeLa, using IKBKE antibody at 1:200 dilution. Rabbit IgG Hu ICC/IF, P, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

IKBKE / IKKE antibody

Western Blot: IKBKE Antibody (6B4B5) [NBP1-47515] - Western blot analysis using IKBKE monoclonal antiobdy against truncated IKBKE recombinant protein(1) and full-length IKBKE(aa1-716)-hIgGFc transfected COS7 cell lysate(2). Mouse IgG1 6B4B5 Purified Hu E, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

IKBKE / IKKE antibody

IKBKE / IKKE Rabbit Aff - Purified Hu E, P, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

Items 1 to 20 of 28 total

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