IKKA antibody

Principal name

IKKA antibody

Alternative names for IKKA antibody

CHUK, TCF16, I kappa-B kinase alpha, IkBKA, IKK-alpha, IKK-A, IkappaB kinase, I-kappa-B kinase 1, NFKBIKA, IKK1, Inhibitor of nuclear factor kappa-B kinase subunit alpha, Conserved helix-loop-helix ubiquitous kinase, Nuclear factor NF-kappa-B inhibitor kinase alpha

SwissProt ID

O15111 (Human), Q5ZJB4 (Chick), Q60680 (Mouse), Q6GM53 (Xenla), Q95KV1 (Bovin)

Gene ID

1147 (CHUK)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey), Bov (Bovine), Por (Porcine), Chk (Chicken), Ze (Zebrafish), Prim (Primate)

Available hosts

Mouse, Rabbit, Goat

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Flow Cytometry (F), PLA/Proximity ligation assay (PLA), Frozen Sections (C)

Background of IKKA antibody

Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Negatively regulates the pathway by phosphorylating the scaffold protein TAXBP1 and thus promoting the assembly of the A20/TNFAIP3 ubiquitin-editing complex (composed of A20/TNFAIP3, TAX1BP1, and the E3 ligases ITCH and RNF11). Therefore, CHUK plays a key role in the negative feedback of NF-kappa-B canonical signaling to limit inflammatory gene activation. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. In turn, these complexes regulate genes encoding molecules involved in B-cell survival and lymphoid organogenesis. Participates also in the negative feedback of the non-canonical NF-kappa-B signaling pathway by phosphorylating and destabilizing MAP3K14/NIK. Within the nucleus, phosphorylates CREBBP and consequently increases both its transcriptional and histone acetyltransferase activities. Modulates chromatin accessibility at NF-kappa-B-responsive promoters by phosphorylating histones H3 at 'Ser-10' that are subsequently acetylated at 'Lys-14' by CREBBP. Additionally, phosphorylates the CREBBP-interacting protein NCOA3.

General readings

1. Huang CC  et al. Intermolecular binding between TIFA-FHA and TIFA-pT mediates tumor necrosis factor alpha stimulation and NF-?B activation. Mol Cell Biol 32:2664-73 (2012).
2. Prager GW  et al. Urokinase (uPA) mediates endothelial cell survival via induction of the X-linked inhibitor of apoptosis protein (XIAP). Blood : (2008). WB .

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

IKKA antibody

Immunoperoxidase of monoclonal antibody to CHUK on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml] Mouse IgG1 2G4 Purified Hu E, ICC/IF, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

IKKA antibody

Western blot analysis of IKK alpha in HeLa whole cell lysate with IKK alpha antibody at 1:1000 dilution. Rabbit IgG Aff - Purified Hu E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

IKKA antibody

Western blot analysis of IKK alpha in HeLa whole cell lysate with IKK alpha antibody at 1:500 dilution. Rabbit IgG Aff - Purified Hu E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

IKKA antibody

Western blot analysis of IKK alpha in Jurkat whole cell lysate with IKK alpha antibody (C3) at 1:500 dilution. Rabbit IgG Aff - Purified Hu E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

IKKA (C-term) antibody

Anti-IKKa antibody was diluted 1:500 to detect IKKa in human kidney tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain. Rabbit Serum Hu, Ms, Rt E, IP, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

IKKA antibody

Predicted band size: 85 kDa . Positive control: Hela, 293 and 3T3 cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane1,2,3: Hela, 293 and 3T3 cells lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

IKKA antibody

Western blot analysis of IKK-alpha expression in EGF treated 293 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

IKKA antibody

Western blot analysis of IKK-alpha/beta expression in HepG2 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

IKKA antibody

Western blot analysis of IKK-alpha/beta expression in Jurkat whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

IKKA antibody

TA326719 (1µg/ml) staining of HeLa nuclear lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

IKKA antibody

Western blot analysis of extracts of various cell lines, using CHUK antibody. Rabbit IgG Purified Hu, Ms
0.1 mg / €325.00
  OriGene Technologies, Inc.

IKKA antibody

Western Blot: IKK alpha Antibody (14A231) [TA336452] - analysis of IKK alpha in Daudi cell lysate using IKK alpha antibody at 1 ug/ml. Mouse IgG1 14A231 Purified Hu, Ms, Prim F, ICC/IF, IP, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

IKKA antibody

Western blot analysis using CHUK mouse mAb against truncated Trx-CHUK recombinant protein (Lane 1). Mouse IgG1 7C10B6 Ascites Hu E, WB
0.1 ml / €360.00
  Acris Antibodies GmbH

IKKA antibody

Western blot analysis using CHUK antibody Cat.-No AM06512SU-N against Raji (1), Jurkat (2) and THP-1 (3) cell lysate. Mouse IgG1 3G12 Ascites Hu E, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

IKKA antibody

Immunofluorescence analysis of NIH/3T3 cells using CHUK antibody Cat.-No AM06518SU-N (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Mouse IgG1 3G12H9 Ascites Hu E, F, ICC/IF, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

IKKA pThr23 antibody

IKKA Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

IKKA pThr23 antibody

Figure 1. Immunohistochemical analysis of paraffin- embedded human colon carcinoma tissue, using IKKα (phospho-Thr23) antibody AP02417PU. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 ml / €320.00
  Acris Antibodies GmbH

IKKA pThr23 antibody

Figure 1. Immunohistochemical analysis of paraffin- embedded human colon carcinoma tissue, using IKKα (phospho-Thr23) antibody AP02417PU. Rabbit Aff - Purified P, WB
50 µl / €240.00
  Acris Antibodies GmbH

IKKA antibody

Figure 1. Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue, using IKKα antibody AP02652PU. Rabbit Aff - Purified P, WB
0.1 ml / €270.00
  Acris Antibodies GmbH

IKKA antibody

Figure 1. Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue, using IKKα antibody AP02652PU. Rabbit Aff - Purified P, WB
50 µl / €230.00
  Acris Antibodies GmbH

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