IKKA antibody

Principal name

IKKA antibody

Alternative names for IKKA antibody

CHUK, TCF16, I kappa-B kinase alpha, IkBKA, IKK-alpha, IKK-A, IkappaB kinase, I-kappa-B kinase 1, NFKBIKA, IKK1, Inhibitor of nuclear factor kappa-B kinase subunit alpha, Conserved helix-loop-helix ubiquitous kinase, Nuclear factor NF-kappa-B inhibitor kinase alpha

SwissProt ID

O15111 (Human), Q5ZJB4 (Chick), Q60680 (Mouse), Q6GM53 (Xenla), Q95KV1 (Bovin)

Gene ID

1147 (CHUK)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey), Bov (Bovine), Por (Porcine), Chk (Chicken), Ze (Zebrafish), Prim (Primate)

Available hosts

Rabbit, Mouse, Goat

Available applications

Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Enzyme Immunoassay (E), Paraffin Sections (P), Flow Cytometry (F)

Background of IKKA antibody

NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization sigl of NF-kB thereby preventing translocation of NF-kB to the nucleus. Exterl stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated. IkB proteins are phosphorylated by IkB kise complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g. In vitro, IKK1/a and IKK2/b can form homo- and heterodimers that can phosphorylate IkBs at the regulatory serine residues directly. IKK1/a and IKK2/b are phosphorylated by NF-kB-inducing kise (NIK) and MAP kise kise kise-1 (MEKK1), respectively. Targeted disruption of IKK1/a gene in mice results in skin and limb abnormalities and death of newborns.

General readings

1. Huang CC  et al. Intermolecular binding between TIFA-FHA and TIFA-pT mediates tumor necrosis factor alpha stimulation and NF-?B activation. Mol Cell Biol 32:2664-73 (2012).
2. Prager GW  et al. Urokinase (uPA) mediates endothelial cell survival via induction of the X-linked inhibitor of apoptosis protein (XIAP). Blood : (2008). WB .

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

IKKA antibody

  Rabbit Polyclonal IKK alpha Antibody  
Western blot analysis of IKK alpha in HeLa whole cell lysate with IKK alpha antibody at 1:1000 dilution. Rabbit IgG Aff - Purified Hu E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

IKKA antibody

  Rabbit Polyclonal IKK alpha Antibody  
Western blot analysis of IKK alpha in HeLa whole cell lysate with IKK alpha antibody at 1:500 dilution. Rabbit IgG Aff - Purified Hu E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

IKKA antibody

  Rabbit Polyclonal IKK alpha Antibody  
Western blot analysis of IKK alpha in Jurkat whole cell lysate with IKK alpha antibody (C3) at 1:500 dilution. Rabbit IgG Aff - Purified Hu E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

IKKA antibody

  Rabbit polyclonal IKK-alpha (Ser176)/IKK-beta (Phospho-Ser177) antibody(Phospho-specific)  
Western blot analysis of extracts from 3T3 cells, treated with TNF (20ng/ml, 30mins), using IKK-a (Phospho-Ser176)/IKK-Ã?(Phospho-Ser177) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

IKKA (C-term) antibody

  Rabbit polyclonal IKK alpha antibody  
Anti-IKKa antibody was diluted 1:500 to detect IKKa in human kidney tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain. Rabbit Serum Hu, Ms, Rt E, IP, WB
0.1 ml / €356.00
  OriGene Technologies, Inc.

IKKA antibody

  Anti-CHUK (Phospho-Thr23) Rabbit Polyclonal Antibody  
Predicted band size: 85 kDa . Positive control: 293 cells treated with serum lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane1: 293 cells untreated with serum lysate Lane2: 293 cells treated with serum lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

IKKA antibody

  Anti-CHUK Rabbit Polyclonal Antibody  
Predicted band size: 85 kDa . Positive control: Hela, 293 and 3T3 cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane1,2,3: Hela, 293 and 3T3 cells lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

IKKA antibody

  Rabbit Polyclonal IKK-alpha Antibody  
Western blot analysis of IKK-alpha expression in EGF treated 293 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

IKKA antibody

  Rabbit Polyclonal IKK-alpha/beta Antibody  
Western blot analysis of IKK-alpha/beta expression in HepG2 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

IKKA antibody

  Rabbit Polyclonal IKK-alpha/beta Antibody  
Western blot analysis of IKK-alpha/beta expression in Jurkat whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

IKKA antibody

  Goat Anti-CHUK Antibody  
TA326719 (1µg/ml) staining of HeLa nuclear lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

IKKA antibody

  Rabbit anti-CHUK Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using CHUK antibody. Rabbit IgG Purified Hu, Ms
0.1 mg / €325.00
  OriGene Technologies, Inc.

IKKA antibody

  Mouse Monoclonal IKK alpha Antibody (14A231)  
Western Blot: IKK alpha Antibody (14A231) [TA336452] - analysis of IKK alpha in Daudi cell lysate using IKK alpha antibody at 1 ug/ml. Mouse IgG1 14A231 Purified Hu, Ms, Prim F, ICC/IF, IP, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

IKKA antibody

  Rabbit Polyclonal Anti-CHUK Antibody  
Gel: 6%SDS-PAGE, Lysate: 50 ug, Lane: Hela cells, Primary antibody: (CHUK Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 minutes Rabbit IgG Purified Hu, Ms E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

IKKA antibody

Western blot analysis using CHUK mouse mAb against truncated Trx-CHUK recombinant protein (Lane 1). Mouse IgG1 7C10B6 Ascites Hu E, WB
0.1 ml / €360.00
  Acris Antibodies GmbH

IKKA antibody

Western blot analysis using CHUK antibody Cat.-No AM06512SU-N against Raji (1), Jurkat (2) and THP-1 (3) cell lysate. Mouse IgG1 3G12 Ascites Hu E, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

IKKA antibody

Immunofluorescence analysis of NIH/3T3 cells using CHUK antibody Cat.-No AM06518SU-N (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Mouse IgG1 3G12H9 Ascites Hu E, F, ICC/IF, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

IKKA pThr23 antibody

IKKA Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

IKKA pThr23 antibody

Figure 1. Immunohistochemical analysis of paraffin- embedded human colon carcinoma tissue, using IKKα (phospho-Thr23) antibody AP02417PU. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 ml / €320.00
  Acris Antibodies GmbH

IKKA pThr23 antibody

Figure 1. Immunohistochemical analysis of paraffin- embedded human colon carcinoma tissue, using IKKα (phospho-Thr23) antibody AP02417PU. Rabbit Aff - Purified P, WB
50 µl / €240.00
  Acris Antibodies GmbH

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