IL1F6 / IL1E antibody
Principal name
IL1F6 / IL1E antibody
Alternative names for IL1F6 / IL1E antibody
FIL1E, Interleukin-1 epsilon, FIL1 epsilon, IL-1E, Interleukin-1 family member 6
Gene ID
27179 (IL36A)
Ncbi ID
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)
Background of IL1F6 / IL1E antibody
IL-36A is is a member of the interleukin 1 cytokine family whose gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2 (1). IL-36A is thought to activate the NF-kappaB pathway through IL-1 receptor family members IL-1RL2 and IL-1RAcP (2). Like the related proteins IL-36B and IL-36G, IL-36A requires post-translatiol processing for full agonist activity, but the cleavage mechanism is currently unknown (3). The IL-36 cytokines have been suggested to amplify Th1 responses by enhancing proliferation and Th1 polarization of ive CD4+ T cells (4).
Western Blot analysis of IL1F6 expression in transfected 293T cell line (H00027179-T01) by IL1F6 MaxPab polyclonal antibody.Lane 1: IL1F6 transfected lysate(17.38 KDa).Lane 2: Non-transfected lysate.
Anti-IL1F6 mouse monoclonal antibody (GTX84297) immunofluorescent staining of COS7 cells transiently transfected with IL1F6
HEK293T cells transfected with either RC219328 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-IL1F6 antibody(GTX84297), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL1F6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL1F6.
Anti-IL1F6 mouse monoclonal antibody (GTX84298) immunofluorescent staining of COS7 cells transiently transfected with IL1F6
HEK293T cells transfected with either RC219328 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-IL1F6 antibody(GTX84298), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL1F6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL1F6.
Anti-IL1F6 mouse monoclonal antibody (GTX84299) immunofluorescent staining of COS7 cells transiently transfected with IL1F6
HEK293T cells transfected with either RC219328 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-IL1F6 antibody(GTX84299), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL1F6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL1F6.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL1F6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL1F6.
Anti-IL1F6 mouse monoclonal antibody (GTX84300) immunofluorescent staining of COS7 cells transiently transfected with IL1F6
Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 24173-1-AP( IL1F6 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 24173-1-AP( IL1F6 Antibody) at dilution of 1:50 (under 40x lens)
Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 24173-1-AP( IL1F6 Antibody) at dilution of 1:1000
Anti-IL1F6 mouse monoclonal antibody (TA501407) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY IL1F6(RC219328).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL1F6 (RC219328, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL1F6.
HEK293T cells transfected with either RC219328 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-IL1F6 antibody(TA501407), and then analyzed by flow cytometry.
Anti-IL1F6 mouse monoclonal antibody (TA501427) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY IL1F6(RC219328).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL1F6 (RC219328, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL1F6.
Anti-IL1F6 mouse monoclonal antibody (TA501428) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY IL1F6(RC219328).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL1F6 (RC219328, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL1F6.
HEK293T cells transfected with either RC219328 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-IL1F6 antibody(TA501428), and then analyzed by flow cytometry.
Anti-IL1F6 mouse monoclonal antibody (TA501459) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY IL1F6(RC219328).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL1F6 (RC219328, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL1F6.
Western blot analysis of extracts (10ug) from 1 cell line by using anti-IL1F6 monoclonal antibody.(1:200)
HEK293T cells transfected with either RC219328 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-IL1F6 antibody(TA501459), and then analyzed by flow cytometry.
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA501459 | IL1F6 / IL1E antibody |
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Mouse | IgG1 | OTI3A2 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€379.00
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| OriGene Technologies, Inc. | |||||||
 +3 additional images |
Highly validated antibody!
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| TA326665 | IL1F6 / IL1E antibody |
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Rabbit | IgG | Purified | Hu | WB |
0.1 mg /
€325.00
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| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| TA501407 | IL1F6 / IL1E antibody |
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 |
Mouse | IgG1 | OTI1A4 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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| TA501427 | IL1F6 / IL1E antibody |
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 |
Mouse | IgG1 | OTI3A12 | Purified | Hu | ICC/IF, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| TA501428 | IL1F6 / IL1E antibody |
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 |
Mouse | IgG1 | OTI1D11 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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| H00027179-B01P | IL1F6 / IL1E antibody |
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Mouse | Purified | Hu | E(detection), WB |
50 µg /
€370.00
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| Abnova Taiwan Corp. | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP319328 | IL1F6 / IL1E |
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
Regular Price: 20 µg / €680.00 Special Price: 20 µg / €398.00 |
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| OriGene Technologies, Inc. | |||||
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| H00027179-P01-10 | IL1F6 / IL1E |
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Human | Purified | 12.5% SDS-PAGE Stained with Coomassie Blue. |
10 µg /
€380.00
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| Abnova Taiwan Corp. | |||||
| H00027179-P01-25 | IL1F6 / IL1E |
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Human | Purified | 12.5% SDS-PAGE Stained with Coomassie Blue. |
25 µg /
€510.00
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| Abnova Taiwan Corp. | |||||
| H00027179-Q01-10 | IL1F6 / IL1E |
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Human | Purified | 12.5% SDS-PAGE Stained with Coomassie Blue. |
10 µg /
€380.00
|
|
| Abnova Taiwan Corp. | |||||
| H00027179-Q01-25 | IL1F6 / IL1E |
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Human | Purified | 12.5% SDS-PAGE Stained with Coomassie Blue. |
25 µg /
€510.00
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| Abnova Taiwan Corp. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| H00027179-T01 | IL1F6 293T Cell Transient Overexpression Lysate(Denatured) |
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0.1 ml /
€270.00
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| Abnova Taiwan Corp. | ||
| LY415288 | IL36A overexpression lysate |
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0.1 mg /
€280.00
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| OriGene Technologies, Inc. |
