ING1 antibody

Principal name

ING1 antibody

Alternative names for ING1 antibody

Inhibitor of growth protein 1, ING-1

SwissProt ID

Q9QXV3 (Mouse), Q9UK52 (Human), Q9UK53 (Human)

Gene ID

3621 (ING1), 26356 (Ing1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Ze (Zebrafish), African clawed frog, Mky (Monkey), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Goat, Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), EPair for Elisa (EPair), ELISA (detection) (E(detection)), Immunoprecipitation (IP)

Background of ING1 antibody

Kit Component:
- KN308326G1, Ing1 gRNA vector 1 in pCas-Guide vector
- KN308326G2, Ing1 gRNA vector 2 in pCas-Guide vector
- KN308326D, donor vector containing left and right homologous arms and GFP-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

1. Campos,E.I., Chin,M.Y., Kuo,W.H. and Li,G. (2004) Biological functions of the ING family tumor suppressors. Cell. Mol. Life Sci. 61 (19-20). 2597-2613.
2. Cheung,K.J. Jr. and Li,G. (2002) p33(ING1) enhances UVB-induced apoptosis in melanoma cells. Exp. Cell Res. 279 (2), 291-298.
3. Vieyra,D. et al (2002) Human ING1 proteins differentially regulate histone acetylation. J. Biol. Chem. 277 (33), 29832-29839.
4. Nagashima,M. et al (2001) DNA damage-inducible gene p33ING2 negatively regulates cell proliferation through acetylation of p53. Proc. Natl. Acad. Sci. U.S.A. 98 (17), 9671-9676.
5. Shinoura,N. et al (1999) Adenovirus-mediated transfer of p33ING1 with p53 drastically augments apoptosis in gliomas. Cancer Res. 59 (21), 5521-5528.
6. Garkavtsev,I., Grigorian,I.A., Ossovskaya,V.S., Chernov,M.V., Chumakov,P.M. and Gudkov,A.V. (1998) The candidate tumour suppressor p33ING1 cooperates with p53 in cell growth control. Nature 391 (6664), 295-298.
7. Helbing,C.C., Veillette,C., Riabowol,K., Johnston,R.N. and Garkavtsev,I. (1997) A novel candidate tumor suppressor, ING1, is involved in the regulation of apoptosis. Cancer Res. 57 (7), 1255-1258.
8. Garkavtsev,I. and Riabowol,K. (1997) Extension of the replicative life span of human diploid fibroblasts by inhibition of the p33ING1 candidate tumor suppressor.
Mol. Cell. Biol. 17 (4), 2014-2019.
9. Garkavtsev,I., Demetrick,D. and Riabowol,K. (1997) Cellular localization and chromosome mapping of a novel candidate tumor suppressor gene (ING1).
Cytogenet. Cell Genet. 76 (3-4), 176-178.
10. Garkavtsev,I., Kazarov,A., Gudkov,A. and Riabowol,K. (1996) Suppression of the novel growth inhibitor p33ING1 promotes neoplastic transformation. Nat. Genet. 14 (4), 415-420.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ING1 antibody

  Rabbit Polyclonal ING1 Antibody  
Immunohistochemical staining of human gall bladder shows nuclear and cytoplasmic positivity in glandular cells.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only. Rabbit IgG Purified Hu, Mky, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ING1 antibody

  Goat polyclonal anti-p33 ING1 antibody  
WB analysis using Anti-p33 ING1 antibody to detect over expressed Human ING1 present in cell nuclear extracts. This WB shows reactivity with purified recombinant GST tagged human ING1b (lane 1) and ING1c protein (lane 2). No reactivity is seen against GST or other ING proteins (not shown). Reactivity of this polyclonal antibody is similar to the specificity of a positive control monoclonal antibody (not shown). The blot was incubated with a 1:1,000 dilution of the antibody at RT. Goat Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

ING1 antibody

  Rabbit Polyclonal Anti-p33 ING1 Antibody  
Western blot analysis of p33 ING1 in mouse brain extract using the antibody Rabbit IgG Serum Hu, Ms E, ICC/IF, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

ING1 antibody

  Rabbit Polyclonal Anti-ING1 Antibody  
WB Suggested Anti-ING1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Hela cell lysate.ING1 is strongly supported by BioGPS gene expression data to be expressed in HeLa Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

ING1 antibody

  Rabbit Polyclonal Anti-ING1 Antibody  
Lanes: 1: 30 ug HeLa lysate, 2: 30 ug HFF lysate, 3: 30 ug U2OS lysate; Primary Antibody Dilution: 1: 1000; Secondary Antibody: Anti-rabbit HRP; Secondary Antibody Dilution: 1: 5000; Gene Name: ING1; Submitted by: Dr. Hyung Suk Oh, Knipe Lab, Harvard Medi Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ze WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ING1 antibody

  Rabbit Polyclonal Anti-ING1 Antibody  
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-4: Hela cells, mouse brain tissue, Jurkat cells, Raw264.7 cells, Primary antibody: (ING1 Antibody) at dilution 1/267, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 second Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ING1 antibody

Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Brain, cortex. Goat Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

ING1 Control Peptide

ING1 Purified
50 µg / €200.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

Ing1 - mouse gene knockout kit via CRISPR

Ing1 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Ing1 - mouse gene knockout kit via CRISPR

Ing1 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

ING1 (transcript variant 1)

  Recombinant protein of human inhibitor of growth family, member 1 (ING1), transcript variant 1  
ING1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

ING1 (full length, N-term HIS tag, transcript variant 2)

  Purified recombinant protein of Human inhibitor of growth family, member 1 (ING1), transcript variant 2, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug  
ING1 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

ING1 (1-279, His-tag)

ING1 Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

ING1 (1-279, His-tag)

ING1 Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

Lysates

Catalog No.    

ING1 overexpression lysate

ING1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

ING1 overexpression lysate

ING1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

ING1 overexpression lysate

ING1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, ING1 (mGFP-tagged) - Human inhibitor of growth family, member 1 (ING1), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, ING1 (mGFP-tagged) - Human inhibitor of growth family, member 1 (ING1), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €960.00
  OriGene Technologies, Inc.

Lenti ORF particles, ING1 (mGFP-tagged) - Human inhibitor of growth family, member 1 (ING1), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, ING1 (mGFP-tagged) - Human inhibitor of growth family, member 1 (ING1), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, ING1 (mGFP-tagged)-Human inhibitor of growth family, member 1 (ING1), transcript variant 3, 200 uL, >10^7 TU/mL

Lenti ORF particles, ING1 (mGFP-tagged)-Human inhibitor of growth family, member 1 (ING1), transcript variant 3, 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

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