ING3 antibody

Principal name

ING3 antibody

Alternative names for ING3 antibody

HSPC301, Inhibitor of growth protein 3, p47ING3

SwissProt ID

Q498T3 (Rat), Q5ZK36 (Chick), Q7ZX31 (Xenla), Q8VEK6 (Mouse), Q9HC99 (Human), Q9NXR8 (Human)

Gene ID

54556 (ING3)

Available reactivities

Can (Canine), Chk (Chicken), Hu (Human), Mky (Monkey), Ms (Mouse), Rt (Rat), Chimp (Chimpanzee), Bov (Bovine), Ze (Zebrafish), African clawed frog, Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Ye (Yeast)

Available hosts

Goat, Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP)

Background of ING3 antibody

ING3 is similar to ING1, a tumor suppressor protein that can interact with TP53, inhibit cell growth, and induce apoptosis. This protein contains a PHD-finger, which is a common motif in proteins involved in chromatin remodeling. This gene can activate p53 trans-activated promoters, including promoters of p21/waf1 and bax. Overexpression of this gene has been shown to inhibit cell growth and induce apoptosis. Allelic loss and reduced expression of this gene were detected in head and neck cancers.The protein encoded by this gene is similar to ING1, a tumor suppressor protein that can interact with TP53, inhibit cell growth, and induce apoptosis. This protein contains a PHD-finger, which is a common motif in proteins involved in chromatin remodeling. This gene can activate p53 trans-activated promoters, including promoters of p21/waf1 and bax. Overexpression of this gene has been shown to inhibit cell growth and induce apoptosis. Allelic loss and reduced expression of this gene were detected in head and neck cancers. Two alternatively spliced transcript variants encoding different isoforms have been observed.

General readings

Gunduz,M., (2008) Cancer Sci. 99 (3), 531-538

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ING3 antibody

WB using anti-ING3 antibody shows detection of a band at ~55 kDa corresponding to ING3 in RKO cells transfected with ING3 (lane 2). Control RKO cells do not show detection of this specific band (lane 1). The identity of the non-specific bands at 33 kDa and 20 kDa has not been determined. Primary antibody was used at 1:1,000. Secondry antibody was a 1:20,000 dilution of IRDye™800 conjugated Rb-a-Goat IgG [H&L]. Goat Aff - Purified Can, Chk, Hu, Mky, Ms, Rt E
0.1 mg / €325.00
  OriGene Technologies, Inc.

ING3 antibody

Western blot analysis of extracts of various cell lines, using ING3 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

ING3 antibody

WB Suggested Anti-ING3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

ING3 antibody

WB Suggested Anti-ING3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human heart Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ye, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

ING3 antibody

AP07597PU-N ING3 antiboy staining of Formalin-Fixed Paraffin-Embedded Human Brain, Cortex at 2.5 µg/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Goat Aff - Purified Chk, Hu, Ms E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

ING3 Control Peptide

ING3 Purified
50 µg / €190.00
  Acris Antibodies GmbH

ING3 antibody

ING3 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

ING3 antibody

ING3 monoclonal antibody (M01), clone 2E9. Western Blot analysis of ING3 expression in Hela NE ( Cat # L013V3 ). Mouse IgG2a 2E9 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ING3 antibody

Immunofluorescence of monoclonal antibody to ING3 on HeLa cell. [antibody concentration 10 ug/ml] Mouse IgG2b 2C4 Purified Hu, Rt E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ING3 antibody

ING3 Mouse IgG2a 2D8 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ING3 antibody

ING3 Mouse IgG2a 2E21 Hu E, WB
50 µg / €370.00
  Abnova Taiwan Corp.

ING3 antibody

ING3 Mouse IgG2a 2E21 Hu E, WB
0.2 ml / €370.00
  Abnova Taiwan Corp.

ING3 antibody

Western blot using ING3 polyclonal antibody ( Cat # PAB10081 ) shows detection of a band at ~55 kDa corresponding to ING3 in RKO cells transfected with ING3 ( lane 2 ).
Control RKO cells do not show detection of this specific band ( lane1 ).
The identity of the non-specific bands at 33kDa and 20 kDa has not been determined.
Each lane contains approximately 10 µg of RKO whole cell lysate ( ATCC# CRL-2577 - human coloncancer ) separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred to nitrocellulose.
After blocking with 5% NF dry milk, the membranewas probed with the primary antibody diluted to1:1,000.
Incubation was at 4°C overnight followed by washes and reaction with a 1:20,000 dilution of IRDyeTM800 conjugated Rb-a-Goat IgG[H&L] MXHu for 45 min at room temperature.
IRDyeTM800 fluorescence imagewas captured using the Odyssey® Infrared Imaging System developed by LI-COR.
IRDye isa trademark of LI-COR, Inc. Chimp, Hu, Rt
0.1 mg / €560.00
  Abnova Taiwan Corp.

ING3 antibody

Human HepG2; WB Suggested Anti-ING3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: HepG2 cell lysate; ING3 antibody - N-terminal region (ARP33346_P050) in Human HepG2 cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

ING3 antibody

Human heart; WB Suggested Anti-ING3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human heart; ING3 antibody - middle region (ARP33347_P050) in Human heart cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

ING3

ING3 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

ING3

ING3 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

ING3

ING3 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

ING3

ING3 Human Purified
10 µg / €380.00
  Abnova Taiwan Corp.

ING3

ING3 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

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