INSIG1 antibody
Principal name
INSIG1 antibody
Alternative names for INSIG1 antibody
INSIG-1, Insulin-induced gene 1 protein
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Paraffin Sections (P)
Background of INSIG1 antibody
Oxysterols regulate cholesterol homeostasis through the liver X receptor (LXR)- and sterol regulatory element-binding protein (SREBP)-mediated signaling pathways. This gene is an insulin-induced gene. INSIG1 is an endoplasmic reticulum (ER) membrane protein that plays a critical role in regulating cholesterol concentrations in cells. INSIG1 binds to the sterol-sensing domains of SREBP cleavage-activating protein (SCAP) and HMG CoA reductase, and is essential for the sterol-mediated trafficking of the two proteins. Oxysterols regulate cholesterol homeostasis through liver X receptor (LXR) and sterol regulatory element-binding protein (SREBP) mediated signaling pathway. This gene is an insulin-induced gene. It encodes an endoplasmic reticulum (ER) membrane protein that plays a critical role in regulating cholesterol concentrations in cells. This protein binds to the sterol-sensing domains of SREBP cleavage-activating protein (SCAP) and HMG CoA reductase, and is essential for the sterol-mediated trafficking of the two proteins. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
General readings
Roth,A., (2008) Mol. Pharmacol. 73 (4), 1282-1289
Western blot analysis of INSIG1 in HepG2 lyaste using INSIG1 polyclonal antibody ( Cat # PAB12411 ).
Western blot analysis of Insig1 expression in Jurkat cell lysate.
Human 721_B; Host: Rabbit. Target Name: WT1 . Sample Tissue: 721_B . Antibody Dilution: 1.0ug/ml. INSIG2 is supported by BioGPS gene expression data to be expressed in 721_B.; INSIG1 antibody - middle region (ARP40166_P050) in Human 721_B cells using Western Blot
Human Muscle; WB Suggested Anti-INSIG1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human Muscle; INSIG1 antibody - middle region (ARP40166_P050) in Human Muscle cells using Western Blot
Human Lung; WB Suggested Anti-INSIG1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human Lung; INSIG1 antibody - middle region (ARP40162_P050) in Human Lung cells using Western Blot
Human Liver; Immunohistochemistry with Liver tissue at an antibody concentration of 10ug/ml using anti-INSIG1 antibody (ARP40159_P050); INSIG1 antibody - middle region (ARP40159_P050) in Human Liver cells using Immunohistochemistry
Human Spleen; WB Suggested Anti-INSIG1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Spleen; INSIG1 antibody - middle region (ARP40159_P050) in Human Spleen cells using Western Blot
Human putamen, Rat cortex; . Lanes: Lane1: 50 ug human putamen lysate. Lane2: 50 ug rat cortex lysate. Primary Antibody Dilution: 1:1000. Secondary Antibody: Mouse anti-rabbit HRP. Secondary Antibody Dilution: 1:15000. Gene Name: INSIG1. Submitted by: Anonymous.; INSIG1 antibody - middle region (ARP40159_P050) in Human putamen, Rat cortex cells using Western Blot
Human Brain; WB Suggested Anti-INSIG1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human brain; INSIG1 antibody - middle region (ARP40161_P050) in Human Brain cells using Western Blot
Detection of Insig 1 in HepG2 lyaste
Western blot analysis of InSig1 expression in Jurkat cell lysate.
WB Suggested Anti-INSIG1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Human Spleen
Lanes: Lane1: 50 ug human putamen lysate; Lane2: 50 ug rat cortex lysate; Primary Antibody Dilution: 1: 1000; Secondary Antibody: Mouse anti-rabbit HRP; Secondary Antibody Dilution: 1: 15000; Gene Name: INSIG1;
Immunohistochemistry with Liver tissue at an antibody concentration of 10 ug/ml using anti-INSIG1 antibody
WB Suggested Anti-INSIG1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Human brain
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA301693 | INSIG1 antibody |
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| Rabbit Polyclonal Antibody against Insig1 | |||||||
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Rabbit | IgG | Purified | Hu | WB |
0.1 ml /
€325.00
|
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| OriGene Technologies, Inc. | |||||||
| TA318983 | INSIG1 antibody |
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| Rabbit polyclonal anti-Insig1 antibody | |||||||
 |
Rabbit | Purified | Bov, Hst, Hu, Ms, Rt | WB |
0.1 mg /
€325.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA345657 | INSIG1 antibody |
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| Rabbit Polyclonal Anti-INSIG1 Antibody | |||||||
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Rabbit | IgG | Purified | Bov, Can, Eq, GP, Gt, Hu, Ms, Por, Rb, Rt, Sh, Ze | P, WB |
50 µg /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
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| TA345658 | INSIG1 antibody |
||||||
| Rabbit Polyclonal Anti-INSIG1 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | WB |
50 µg /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA345659 | INSIG1 antibody |
||||||
| Rabbit Polyclonal Anti-INSIG1 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | WB |
50 µg /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN200312 | INSIG1 - human gene knockout kit via CRISPR |
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Human | GFP-Puro |
1 kit /
€1,200.00
|
| OriGene Technologies, Inc. | |||
| KN200312BN | INSIG1 - human gene knockout kit via CRISPR |
||
|
Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN200312LP | INSIG1 - human gene knockout kit via CRISPR |
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|
Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN200312RB | INSIG1 - human gene knockout kit via CRISPR |
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|
Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Human Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RC200312L2V | Lenti ORF particles, INSIG1 (mGFP-tagged) - Human insulin induced gene 1 (INSIG1), transcript variant 1, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€960.00
|
|
| OriGene Technologies, Inc. | ||
| RC223272L2V | Lenti ORF particles, INSIG1 (mGFP-tagged) - Human insulin induced gene 1 (INSIG1), transcript variant 3, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€880.00
|
|
| OriGene Technologies, Inc. | ||
| RC200312L1V | Lenti ORF particles, INSIG1 (Myc-DDK tagged) - Human insulin induced gene 1 (INSIG1), transcript variant 1 , 200 uL, >10^7 TU/mL |
|
|
200 µl /
€860.00
|
|
| OriGene Technologies, Inc. | ||
| RC223272L1V | Lenti ORF particles, INSIG1 (Myc-DDK tagged) - Human insulin induced gene 1 (INSIG1), transcript variant 3 , 200 uL, >10^7 TU/mL |
|
|
200 µl /
€780.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR206261L2V | Lenti ORF particles, Insig1 (GFP-tagged ORF) - Rat insulin induced gene 1 (Insig1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€880.00
|
|
| OriGene Technologies, Inc. | ||
| RR206261L1V | Lenti ORF particles, Insig1 (Myc-DDK-tagged ORF) - Rat insulin induced gene 1 (Insig1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€780.00
|
|
| OriGene Technologies, Inc. | ||
