INSIG1 antibody
Principal name
INSIG1 antibody
Alternative names for INSIG1 antibody
INSIG-1, Insulin-induced gene 1 protein
Ncbi ID
Available reactivities
Available hosts
Available applications
Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Flow Cytometry (F), Frozen Sections (C)
Background of INSIG1 antibody
Oxysterols regulate cholesterol homeostasis through the liver X receptor (LXR)- and sterol regulatory element-binding protein (SREBP)-mediated signaling pathways. This gene is an insulin-induced gene. INSIG1 is an endoplasmic reticulum (ER) membrane protein that plays a critical role in regulating cholesterol concentrations in cells. INSIG1 binds to the sterol-sensing domains of SREBP cleavage-activating protein (SCAP) and HMG CoA reductase, and is essential for the sterol-mediated trafficking of the two proteins. Oxysterols regulate cholesterol homeostasis through liver X receptor (LXR) and sterol regulatory element-binding protein (SREBP) mediated signaling pathway. This gene is an insulin-induced gene. It encodes an endoplasmic reticulum (ER) membrane protein that plays a critical role in regulating cholesterol concentrations in cells. This protein binds to the sterol-sensing domains of SREBP cleavage-activating protein (SCAP) and HMG CoA reductase, and is essential for the sterol-mediated trafficking of the two proteins. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
General readings
Roth,A., (2008) Mol. Pharmacol. 73 (4), 1282-1289
Detection of Insig 1 in HepG2 lyaste using NB110-55244.
Western blot analysis of INSIG1 in HepG2 lyaste using INSIG1 polyclonal antibody ( Cat # PAB12411 ).
Western blot analysis of Insig1 expression in Jurkat cell lysate.
Western Blot: INSIG-1 Lysate [NBL1-12003] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for INSIG1
Human 721_B; Host: Rabbit. Target Name: WT1 . Sample Tissue: 721_B . Antibody Dilution: 1.0ug/ml. INSIG2 is supported by BioGPS gene expression data to be expressed in 721_B.; INSIG1 antibody - middle region (ARP40166_P050) in Human 721_B cells using Western Blot
Human Muscle; WB Suggested Anti-INSIG1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human Muscle; INSIG1 antibody - middle region (ARP40166_P050) in Human Muscle cells using Western Blot
Human Lung; WB Suggested Anti-INSIG1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human Lung; INSIG1 antibody - middle region (ARP40162_P050) in Human Lung cells using Western Blot
Human Liver; Immunohistochemistry with Liver tissue at an antibody concentration of 10ug/ml using anti-INSIG1 antibody (ARP40159_P050); INSIG1 antibody - middle region (ARP40159_P050) in Human Liver cells using Immunohistochemistry
Human Spleen; WB Suggested Anti-INSIG1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Spleen; INSIG1 antibody - middle region (ARP40159_P050) in Human Spleen cells using Western Blot
Human putamen, Rat cortex; . Lanes: Lane1: 50 ug human putamen lysate. Lane2: 50 ug rat cortex lysate. Primary Antibody Dilution: 1:1000. Secondary Antibody: Mouse anti-rabbit HRP. Secondary Antibody Dilution: 1:15000. Gene Name: INSIG1. Submitted by: Anonymous.; INSIG1 antibody - middle region (ARP40159_P050) in Human putamen, Rat cortex cells using Western Blot
Human Brain; WB Suggested Anti-INSIG1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human brain; INSIG1 antibody - middle region (ARP40161_P050) in Human Brain cells using Western Blot
Immunohistochemical of paraffin-embedded human kidney using 22115-1-AP(INSIG1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human kidney using 22115-1-AP(INSIG1 antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemical of paraffin-embedded human liver using 22115-1-AP(INSIG1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver using 22115-1-AP(INSIG1 antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemical of paraffin-embedded human kidney using 22115-1-AP(INSIG1 antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemical of paraffin-embedded human liver using 22115-1-AP(INSIG1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver using 22115-1-AP(INSIG1 antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemical of paraffin-embedded human kidney using 22115-1-AP(INSIG1 antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of MCF-7 cells using 22115-1-AP( INSIG1 Antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG
HepG2 cells were subjected to SDS PAGE followed by western blot with 22115-1-AP(INSIG1 antibody) at dilution of 1:1000
MCF7 cells were subjected to SDS PAGE followed by western blot with 22115-1-AP(INSIG1 antibody) at dilution of 1:1000
HepG2 cells were subjected to SDS PAGE followed by western blot with 22115-1-AP(INSIG1 antibody) at dilution of 1:1000
MCF7 cells were subjected to SDS PAGE followed by western blot with 22115-1-AP(INSIG1 antibody) at dilution of 1:1000
SGC-7901 cells were subjected to SDS PAGE followed by western blot with 22115-1-AP(INSIG1 antibody) at dilution of 1:1000
Mouse liver tissue were subjected to SDS PAGE followed by western blot with 22115-1-AP(INSIG1 antibody) at dilution of 1:1000
MCF7 cells were subjected to SDS PAGE followed by western blot with 22115-1-AP(INSIG1 antibody) at dilution of 1:1000
A549 cells were subjected to SDS PAGE followed by western blot with 22115-1-AP(INSIG1 antibody) at dilution of 1:1000
IP Result of anti-INSIG1 (IP:22115-1-AP, 4ug; Detection:22115-1-AP 1:1000) with HepG2 cells lysate 3200ug.
Detection of INSIG1 in Fetal Lung using anti-INSIG1 antibody (GTX47436) at 1 µg/mL.
Detection of INSIG1 in Fetal Brain using anti-INSIG1 antibody (GTX47437) at 1 µg/mL.
Immunohistochemistry with liver tissue at an antibody concentration of 10ug/ml using anti-INSIG1 antibody (GTX47437)
Detection of INSIG1 in Fetal Spleen using anti-INSIG1 antibody (GTX47438) at 1 µg/mL.
Western blot using INSIG2 antibody (GTX474380) detected with Mouse anti-rabbit HRP Lanes: Lane 1: 50 ug human putamen lysate Lane 2: 50 ug rat cortex lysate
Western blot analysis of InSig1 expression in Jurkat cell lysate.
INSIG1 Antibody (N-term) (GTX80927) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the INSIG1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Western blot analysis of INSIG1 Antibody (N-term) (GTX80927) in Hela cell line lysates (35ug/lane). INSIG1 (arrow) was detected using the purified Pab.
INSIG1 Antibody (N-term) (GTX80927) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA301693 | INSIG1 antibody |
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 |
Rabbit | IgG | Purified | Hu | WB |
0.1 ml /
€325.00
|
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| OriGene Technologies, Inc. | |||||||
| TA318983 | INSIG1 antibody |
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 |
Rabbit | Purified | Bov, Hst, Hu, Ms, Rt | WB |
0.1 mg /
€325.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA345657 | INSIG1 antibody |
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 |
Rabbit | IgG | Purified | Bov, Can, Eq, GP, Gt, Hu, Ms, Por, Rb, Rt, Sh, Ze | P, WB |
50 µg /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
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| TA345658 | INSIG1 antibody |
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 |
Rabbit | IgG | Purified | Hu, Ms | WB |
50 µg /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA345659 | INSIG1 antibody |
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 |
Rabbit | IgG | Purified | Hu | WB |
50 µg /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| PAB8786 | INSIG1 antibody |
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 |
Rabbit | Bov, Hst, Hu, Ms, Rt | WB |
0.1 mg /
€470.00
|
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| Abnova Taiwan Corp. | |||||||
| PAB12411 | INSIG1 antibody |
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 |
Rabbit | Hu | WB |
0.1 ml /
€470.00
|
|||
| Abnova Taiwan Corp. | |||||||
| ARP40159_P050 | INSIG1 antibody |
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 |
Rabbit | Aff - Purified | African clawed frog, Bov, Can, Chk, Hu, Ms, Por, Rt | WB |
50 µg /
€350.00
|
||
| AVIVA Systems Biology | |||||||
| +2 additional images |
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| ARP40161_P050 | INSIG1 antibody |
||||||
 |
Rabbit | Aff - Purified | Hu | WB |
50 µg /
€350.00
|
||
| AVIVA Systems Biology | |||||||
| ARP40162_P050 | INSIG1 antibody |
||||||
 |
Rabbit | Aff - Purified | Hu | WB |
50 µg /
€350.00
|
||
| AVIVA Systems Biology | |||||||
| ARP40166_P050 | INSIG1 antibody |
||||||
 |
Rabbit | Aff - Purified | African clawed frog, Bov, Can, Chk, Hu, Ms, Por, Rt, Ze | WB |
50 µg /
€350.00
|
||
| AVIVA Systems Biology | |||||||
| +1 additional image |
|||||||
| NB110-55244B | INSIG1 antibody |
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|
Rabbit | Biotin | Hu | WB |
0.1 ml /
€360.00
|
||
| Novus Biologicals Inc. | |||||||
| NB110-55244G | INSIG1 antibody |
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|
Rabbit | DyLight488 | Hu | WB |
0.1 ml /
€390.00
|
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| Novus Biologicals Inc. | |||||||
| NB110-55244H | INSIG1 antibody |
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|
Rabbit | HRP | Hu | WB |
0.1 ml /
€380.00
|
||
| Novus Biologicals Inc. | |||||||
| NB110-55244SS | INSIG1 antibody |
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|
Rabbit | Aff - Purified | Hu | ICC/IF, WB |
25 µl /
€200.00
|
||
| Novus Biologicals Inc. | |||||||
