InsP6 kinase 2 / IP6K2 antibody

Principal name

InsP6 kinase 2 / IP6K2 antibody

Alternative names for InsP6 kinase 2 / IP6K2 antibody

IHPK2, TCCCIA00113, P(i)-uptake stimulator, PiUS, Inositol hexakisphosphate kinase 2

SwissProt ID

Q80V72 (Mouse), Q95221 (Rabit), Q9R0U1 (Rat), Q9UHH9 (Human)

Gene ID

51447 (IP6K2)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Enzyme Immunoassay (E), Immunoprecipitation (IP)

Background of InsP6 kinase 2 / IP6K2 antibody

The members of the inositol hexakisphosphate kinase family, IP6K1 and IP6K2, have a high affinity and selectivity for inositol hexakisphosphate (InsP6) as a substrate. IP6K1 and IP6K2 (also designated PiUS) convert InsP6 to PP-InsP5. However, neither kinase demonstrates any catalytic activity with other inositol pyrophosphates. The presence of InsP6, which inhibits serine/threonine protein phosphatases, increases the influx of calcium across the plasma membrane and implies that it may mediate the regulation of insulin exocytosis. IP6K1 was purified as a 54 kDa protein in rat brain extracts. By homology, IP6K1 and IP6K2 were characterized in mouse as a 50 kDa and 49 kDa protein, respectively. IP6K1 displays ATP synthase activity by transferring a phosphate from PP-InsP5 to ADP, which suggests a role for the IP6 kinases as high energy phosphate donors.

General readings

Fairfax BP.,et al. (2012) Nat. Genet. 44 (5), 502-510

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

InsP6 kinase 2 / IP6K2 antibody

Western blot analysis of extracts from HUVEC cells, Jurkat cells and COLO cells, using IP6K2 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

InsP6 kinase 2 / IP6K2 antibody

Western blot analysis with extracts from A549 cells using InsP6 kinase 2 / IP6K2 antibody (Cat.-No. AP01285PU-N) (1/500). Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

InsP6 kinase 2 / IP6K2 antibody

Immunofluorescence of purified MaxPab antibody to IHPK2 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

InsP6 kinase 2 / IP6K2 antibody

InsP6 kinase 2 / IP6K2 Mouse IgG2a 1C6 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

InsP6 kinase 2 / IP6K2 antibody

Effect of gamma-irradiation and IFN-beta on IP6K2 protein levels.
( a ) NIH-OVCAR-3 cells growing at 75% confluence were trypsinized, suspended in 1 ml PBS, and subject to 0, 1 ( white bars ) or 2 Gy ( gray bars ) irradiation and replated in complete medium.
At 8 and 16 h after plating, cells were lysed and subject to Western blot analysis  using IP6K2 monoclonal antibody, clone 4F10 ( Cat # MAB2177 ).
To control for loading, blots were probed with anti-actin antibody; bands were digitized and quantified.
IP6K2 signal intensity was normalized to actin, then expressed as fold induction, with unirradiated cells representing a protein level of 1.
Purified rIP6K2 was included as a positive control ( lane 1 ).
Numbers to right of blots indicate MW markers.
Only relevant portion of the blots are shown.
( b ) NIH-OVAR-3 cells were grown in the presence of 200 U/ml IFN-beta for 0-48 h and then subject to Western blot analysis and quantified as above. Mouse IgG2b 4F10 Hu WB
0.1 mg / €470.00
  Abnova Taiwan Corp.

InsP6 kinase 2 / IP6K2 antibody

Immunofluorescence analysis of COS7 cells, using IP6K2 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
50 µg / €205.00
  Assay Biotech

InsP6 kinase 2 / IP6K2 antibody

Immunofluorescence analysis of COS7 cells, using IP6K2 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €310.00
  Assay Biotech

InsP6 kinase 2 / IP6K2 antibody

Immunohistochemical analysis of IP6K2 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu ICC/IF, IP, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

InsP6 kinase 2 / IP6K2 antibody

Immunohistochemical analysis of IP6K2 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu ICC/IF, IP, P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

InsP6 kinase 2 / IP6K2 antibody

Immunohistochemical analysis of IP6K2 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu ICC/IF, IP, P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

InsP6 kinase 2 / IP6K2 antibody

InsP6 kinase 2 / IP6K2 Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

InsP6 kinase 2 / IP6K2 antibody

Detection of Abeta 40 on 5 ng of peptide per lane using NB 300-225. Lane 1: Abeta-40, lane 2: Abeta-42, lane 3: Abeta-40 and -42 mix. Mouse IgG2b 4F10 Purified Hu WB
0.1 mg / €480.00
  Novus Biologicals Inc.

InsP6 kinase 2 / IP6K2 antibody

Western Blot:  Inositol Hexakisphosphate Kinase 2 Antibody [NBP1-32322]- (30 µg of whole cell lysate) A:Raji 7.5% SDS PAGE diluted at 1:500 Rabbit IgG Hu ICC/IF, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

InsP6 kinase 2 / IP6K2 antibody

InsP6 kinase 2 / IP6K2 Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

InsP6 kinase 2 / IP6K2 antibody

InsP6 kinase 2 / IP6K2 Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
50 µg / €290.00
  Novus Biologicals Inc.

InsP6 kinase 2 / IP6K2 antibody

Immunohistochemistry-Paraffin: Inositol Hexakisphosphate Kinase 2 Antibody [NBP1-89635] - Immunohistochemical staining of human stomach shows strong nuclear and cytoplasmic positivity in glandular cells. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

InsP6 kinase 2 / IP6K2 (transcript variant 1)

InsP6 kinase 2 / IP6K2 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

InsP6 kinase 2 / IP6K2 (full length, N-term HIS tag, transcript variant 2)

InsP6 kinase 2 / IP6K2 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

InsP6 kinase 2 / IP6K2 (1-426, His-tag)

InsP6 kinase 2 / IP6K2 Human Purified > 85 % by SDS - PAGE E. coli
0.25 mg / €940.00
  Acris Antibodies GmbH

InsP6 kinase 2 / IP6K2 (1-426, His-tag)

InsP6 kinase 2 / IP6K2 Human Purified > 85 % by SDS - PAGE E. coli
50 µg / €370.00
  Acris Antibodies GmbH

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