Insulin antibody

Principal name

Insulin antibody

Alternative names for Insulin antibody

INS

SwissProt ID

P01308 (Human), P01310 (Horse), P01311 (Rabit), P01315 (Pig), P01317 (Bovin), P01318 (Sheep), P01321 (Canfa), P01329 (Cavpo), P06306 (Felca), P67970 (Chick)

Gene ID

3630 (INS), 397415, 483665, 280829, 100009181

Available reactivities

Hu (Human), Bov (Bovine), Por (Porcine), Rb (Rabbit), Rt (Rat), Can (Canine), Eq (Equine), Fe (Feline), GP (Guinea Pig), Sh (Sheep), Ms (Mouse), Ft (Ferret), Hst (Hamster), Deer, Mky (Monkey)

Available hosts

Mouse, Rabbit, Guinea Pig, Chicken, Sheep, Goat

Available applications

Enzyme Immunoassay (E), Radioimmunoassay (R), Frozen Sections (C), Paraffin Sections (P), Functional assay (FN), ELISA (capture) (E(capture)), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Dot blot (Dot), Flow Cytometry (F), Immunodiffusion (ID)

Background of Insulin antibody

Monoclonal Anti-Insulin may be used for the localization of insulin using RIA, dot blot and immunocytochemistry. Insulin is one of the major regulatory hormones of intermediate metabolism throughout the body. The biological actions of this hormone involve integration of carbohydrate, protein, and lipid metabolism. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Immunocytochemical investigations have localized insulin in the B or b-cells of pancreatic islets of Langerhans. Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of b-cell origin such as insulinoma. Insulin-specific antibodies prove useful as b-cell and tumor markers using immunohistochemical techniques, and as analytical tools in quantification of the hormone.

General readings

This product has been used in:Witt S et al. [Production and use of monoclonal glucagon and insulin antibodies--reduction of pancreatic insulin in rats by treatment with complete Freund's adjuvant] Acta Histochem Suppl 35:217-23 (1988). PubMed PMID: 3138717
Keilacker H et al. Kinetic properties of monoclonal insulin antibodies. Biomed Biochim Acta 45:1093-102 (1986). PubMed PMID: 3535787
General / background references:Madsen OD Proinsulin-specific monoclonal antibodies. Immunocytochemical application as beta-cell markers and as probes for conversion. Diabetes 36:1203-11 (1987). PubMed PMID: 2443408

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Insulin antibody

Figure 1. AP08860PU-N Insulin antibody staining of Formalin-Fixed Paraffin-Embedded Human Pancreas at 1/100, followed by biotinylated Goat anti-Rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit IgG Purified Hu, Ms, Rt E, F, ICC/IF, P
50 µl / €470.00
  Acris Antibodies GmbH

Insulin antibody

Formalin-Paraffin Human pancreas stained with Insulin Antibody Cat.-No AM33363PU (Clone E2-E3+2D11-H5). Note cytoplasmic staining of cells. Mouse IgG1 E2-E3+2D11-H5 (INS04+ INS05) Purified Bov, Hu, Por, Rb, Rt C, F, ICC/IF, IP, P, WB
0.2 mg / €390.00
  Acris Antibodies GmbH

Insulin antibody

Formalin-Paraffin Human pancreas stained with Insulin Antibody Cat.-No AM33363PU (Clone E2-E3+2D11-H5). Note cytoplasmic staining of cells. Mouse IgG1 E2-E3+2D11-H5 (INS04+ INS05) Purified Bov, Hu, Por, Rb, Rt C, F, ICC/IF, IP, P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

Insulin antibody

Formalin-Paraffin Human pancreas stained with Insulin Antibody Cat.-No AM33363PU (Clone E2-E3+2D11-H5). Note cytoplasmic staining of cells. Mouse IgG1 E2-E3+2D11-H5 (INS04+ INS05) Purified Bov, Hu, Por, Rb, Rt C, F, ICC/IF, IP, P, WB
20 µg / €160.00
  Acris Antibodies GmbH

Insulin antibody

Formalin-Paraffin Human pancreas stained with Insulin Antibody (Clone SPM139). Note cytoplasmic staining of cells. Mouse IgG1 SPM139 Purified Bov, Hu, Por C, F, ICC/IF, IP, P, WB
0.2 mg / €390.00
  Acris Antibodies GmbH

Insulin antibody

Formalin-Paraffin Human pancreas stained with Insulin Antibody (Clone SPM139). Note cytoplasmic staining of cells. Mouse IgG1 SPM139 Purified Bov, Hu, Por C, F, ICC/IF, IP, P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

Insulin antibody

Formalin-Paraffin Human pancreas stained with Insulin Antibody (Clone SPM139). Note cytoplasmic staining of cells. Mouse IgG1 SPM139 Purified Bov, Hu, Por C, F, ICC/IF, IP, P, WB
20 µg / €160.00
  Acris Antibodies GmbH

Insulin antibody

Formalin-Paraffin Human pancreas stained with Insulin Antibody (Clone SPM531). Note cytoplasmic staining of cells. Mouse IgG1 SPM531 Purified Bov, Hu, Por, Rb, Rt C, F, ICC/IF, IP, P, WB
0.2 mg / €390.00
  Acris Antibodies GmbH

Insulin antibody

Formalin-Paraffin Human pancreas stained with Insulin Antibody (Clone SPM531). Note cytoplasmic staining of cells. Mouse IgG1 SPM531 Purified Bov, Hu, Por, Rb, Rt C, F, ICC/IF, IP, P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

Insulin antibody

Formalin-Paraffin Human pancreas stained with Insulin Antibody (Clone SPM531). Note cytoplasmic staining of cells. Mouse IgG1 SPM531 Purified Bov, Hu, Por, Rb, Rt C, F, ICC/IF, IP, P, WB
20 µg / €160.00
  Acris Antibodies GmbH

Insulin antibody

ELISA Results of Mab anti-Insulin antibody tested against human insulin by ELISA. Each well was coated with 0.1?g of conjugate. The starting concentration of antibody in the dilution series was 10 ?g/ml. Each point on the Y-axis represents a 3-fold dilution. The midpoint of the titration curve represents approx-imately 5ng/ml antibody or a 1:200,000 dilution from the stock concentration. HRP conjugated Gt-a-Mouse IgG H&L(p/n 610-103-121) and TMB substrate were used for detection. Mouse IgG1 2D11.H5 Purified Hu, Por C, E, IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Insulin antibody

Immunohistochemistry staining of Paraffin Pancreas tissue by Insulin antibody (dilution: 1:100 - 1:500; visualization of staining: Cytoplasmic) Guinea Pig Purified Hu P
1 ml / €460.00
  OriGene Technologies, Inc.

Insulin antibody

Immunohistochemistry analysis of Insulin antibody in paraffin-embedded human pancreas tissue. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

Insulin antibody

Insulin Goat IgG Aff - Purified Can, Hu, Mky, Ms, Rt WB
0.6 mg / €320.00
  SICGEN

Insulin antibody

Insulin Mouse IgG1 D4B8 Purified C, E
0.2 mg / €400.00
  Acris Antibodies GmbH

Insulin antibody

Formalin Fixed, Paraffin Embedded Human pancreas stained with Mouse anti Pig Insulin Antibody Cat.-No AM05475PU-N (Clone 2D11). Mouse IgG1 2D11 Purified Bov, Hu, Por E, P
0.2 mg / €320.00
  Acris Antibodies GmbH

Insulin antibody

AM08397HR-N Insulin Antibody stainig of Formalin-Fixed, Paraffin-Embedded Human Pancreas at 10 µg/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Mouse IgG1 8E2 HRP Hu E, P
50 µg / €470.00
  Acris Antibodies GmbH

Insulin antibody

Immunohistochemistry of Human Pancreas (Left): Antigen retrieval was done with Tris-EDTA, pH 9.0 in pressure cooker for 20 minutes. Optimum staining of the islet cell was observed.
Indirect ELISA (Right): Serial dilution of the recombinant insulin was done to check the affinity. The antibody used at 10 µg/ml can sensitively detect antigen up to 4 ng per well. Mouse IgG1 HPI-B5 Aff - Purified Bov, Hu, Por C, E, WB
0.1 mg / €390.00
  Acris Antibodies GmbH

Insulin antibody

Immunohistochemistry on Rat pancreas sections Mouse IgG1 ISL-8J Purified Hu, Ms, Rt P
0.1 mg / €340.00
  Acris Antibodies GmbH

Insulin antibody

 
Immunohistochemical detection of Insulin expressed in β cell in Langerhans islet in pancreas using AM26629AF-N Mouse IgG1 1C5A Azide Free Hu IP, P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

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