Insulin-degrading enzyme antibody

Principal name

Insulin-degrading enzyme antibody

Alternative names for Insulin-degrading enzyme antibody

IDE, EC=3.4.24.56, Insulin protease, Insulinase, Insulysin, Abeta-degrading protease

SwissProt ID

P14735 (Human), P35559 (Rat), Q24K02 (Bovin), Q9JHR7 (Mouse)

Gene ID

3416 (IDE), 15925 (Ide)

Available reactivities

Hu (Human), Rt (Rat), Ms (Mouse), Can (Canine), Prim (Primate), Bov (Bovine), Ze (Zebrafish)

Available hosts

Mouse, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of Insulin-degrading enzyme antibody

Insulysin was identified nearly a century ago as an enzyme responsible for the degradation of insulin in cells, although the precise interactions between insulin and insulysin remain elusive. Human insulysin was cloned in 1988, and shown to be a 118 kDa protein that exists primarily as a homodimer, and perhaps also complexed with other molecules. The sequence is well conserved between humans, rats and mice, and the antibody recognizes these species. Insulysin is a metalloproteinase of the clan ME, family M16, which contains an active site HxxEH, a reversal of the canonical HExxH zinc binding motif. Considered a zinc metalloproteinase, the activity of insulysin can be blocked with EDTA or 1-10 phenanthroline. In addition to the active metalloproteinase domain, insulysin contains a second metalloproteinase site which is considered catalytically inactive, and is thought to assist in substrate binding. Insulysin is most closely related to the bacterial proteinase pitrilysin, (the human orthologue of which appears to be MPRP1) and the mammalian proteinsae nardilysin. Generally thought to be a cytoplasmic protein, insulysin has been isolated from many different tissues and cell lines, and can degrade intact insulin, insulin B chain, glucagon, denatured hemoglobin, alpha amyloid protein, TGF alpha and amylin. Recent work implicates insulysin in clearing beta amyloid plaques from the brain, and has generated much interest in Alzheimer’s disease research. The pH optimum for insulysin is basic, pH 8.5, which also distinguishes it from other metalloproteinases.
Insulin degrading enzyme (IDE) has a preferential affinity for insulin such that the presence of insulin will inhibit IDE mediated degradation of other substrates. IDE degrades a variety of other peptides including atrial natriuretic peptide and amylin. IDE catabolizes A beta and has been implicated as a candidate enzyme responsible for the degradation and clearance of A beta in the brain. IDE has also been shown to degrade the APP intracellular domain (AICD), a product of gamma secretase cleaved APP that may function in nuclear signaling.

General readings

Zhao Z, Xiang Z, Haroutunian V, Buxbaum JD, Stetka B, Pasinetti GM.
Insulin degrading enzyme activity selectively decreases in the hippocampal formation of cases at high risk to develop Alzheimer's disease.
Neurobiol Aging. 2007 Jun;28(6):824-30. Epub 2006 Jun 12.
PMID: 16769157

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Insulin-degrading enzyme antibody

TA302516 (0.1µg/ml) staining of K562 cell lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Insulin-degrading enzyme antibody

TA303255 (1µg/ml) staining of Mouse Brain lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Insulin-degrading enzyme antibody

Western blot analysis of K652 cell using TA323992(IDE Antibody) at dilution 1/350 Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Insulin-degrading enzyme antibody

Western blot analysis of K652 cell using TA323993(IDE Antibody) at dilution 1/350 Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Insulin-degrading enzyme antibody

Western blot analysis of extracts of various cell lines, using IDE antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

Insulin-degrading enzyme antibody

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with IDE antibody (Center) (AP11440PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Insulin-degrading enzyme (914-924) antibody

Immunohistochemistry Image: Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE) Goat Aff - Purified Bear, Bov, Bt, Can, Chk, Elephant, Eq, Hst, Hu, Insect, Mky, Ms, Op, Por, Prim, Rb, Rt, Xen, Ze E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Insulin-degrading enzyme (222-233) antibody

Prostate, Human: Formalin-Fixed, Paraffin-Embedded (FFPE) Goat Aff - Purified Bear, Bt, Can, Elephant, Eq, Hst, Hu, Mky, Ms, Op, Platypus, Por, Prim, Rb, Rt P
50 µg / €470.00
  Acris Antibodies GmbH

Insulin-degrading enzyme (349-605) antibody

Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Aff - Purified Bov, Hu, Ms, Rt, Ze P, WB
50 µl / €470.00
  Acris Antibodies GmbH

Insulin-degrading enzyme antibody

IDE polyclonal antibody (A01), Lot # ABNOVA060711QCS1 Western Blot analysis of IDE expression in K-562 ( Cat # L009V1 ). Mouse Serum Hu, Rt E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

Insulin-degrading enzyme antibody

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with IDE polyclonal antibody ( Cat # PAB3109 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Hu E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

Insulin-degrading enzyme antibody

293 overexpressing IDE and probed with IDE polyclonal antibody ( Cat # PAB7317 ) ( mock transfection in first lane), tested by Origene. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

Insulin-degrading enzyme antibody

The Ide polyclonal antibody ( Cat # PAB7318 ) ( 1 µg/ml ) staining of mouse brain lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Ms, Rt E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

Insulin-degrading enzyme (N-term) antibody

Human HeLa; WB Suggested Anti-IDE Antibody. . Titration: 1.0 ug/ml. . Positive Control: Hela Whole Cell. IDE is strongly supported by BioGPS gene expression data to be expressed in Human HeLa cells.; IDE antibody - N-terminal region (ARP30652_P050) in Human HeLa cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

Insulin-degrading enzyme antibody

Western blot analysis of IDE expression in Hela (A); HepG2 (B) whole cell lysates. Mouse Aff - Purified Hu WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

Insulin-degrading enzyme antibody

Western blot analysis of IDE expression in Hela (A); HepG2 (B) whole cell lysates. Mouse Aff - Purified Hu WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

Insulin-degrading enzyme antibody

Western blot analysis of IDE expression in Hela (A); HepG2 (B) whole cell lysates. Mouse Aff - Purified Hu WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

Insulin-degrading enzyme (42-1019) antibody

Insulin-degrading enzyme Mouse IgG1 334501 Purified Hu, Ms, Prim, Rt F, WB
0.1 mg / €340.00
  Neuromics Antibodies

Insulin-degrading enzyme antibody

NB100-61667 (1ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence Goat IgG Aff - Purified Ms, Rt E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

Insulin-degrading enzyme antibody

Western Blot: -Insulysin / Insulinase Antibody [NB100-68148] - (0.1 ug/ml) staining of K562 cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat IgG Aff - Purified Can, Hu E, P, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

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