Insulin promoter factor 1 antibody

Principal name

Insulin promoter factor 1 antibody

Alternative names for Insulin promoter factor 1 antibody

PDX1, PDX-1, IPF1, Pancreas/duodenum homeobox protein 1, Insulin promoter factor 1, IPF-1, Islet/duodenum homeobox-1, IDX-1, Somatostatin-transactivating factor 1, STF-1, Insulin upstream factor 1, IUF-1, Glucose-sensitive factor, GSF

SwissProt ID

P52945 (Human), P52946 (Mouse), P52947 (Rat), Q6DC85 (Danre)

Gene ID

3651 (PDX1), 18609 (Pdx1), 7456 (WIPF1), 29535

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Chk (Chicken), Por (Porcine), Rt (Rat), Chimp (Chimpanzee), Eq (Equine), Rb (Rabbit), GP (Guinea Pig), Sh (Sheep), Ze (Zebrafish)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Frozen Sections (C), ELISA (detection) (E(detection)), Flow Cytometry (F)

Background of Insulin promoter factor 1 antibody

Activates insulin, somatostatin, glucokinase, islet amyloid polypeptide and glucose transporter type 2 gene transcription. Particularly involved in glucose-dependent regulation of insulin gene transcription. Binds preferentially the DNA motif 5'-[CT]TAAT[TG]-3'. During development, specifies the early pancreatic epithelium, permitting its proliferation, branching and subsequent differentiation. At adult stage, required for maintaining the hormone-producing phenotype of the beta-cell. Insulin promoter factor 1 is an islet-specific protein that activates transcription of the insulin andsomatostatin genes. It is a key regulator of islet peptide hormone expressionn and also plays an essential role in pancreatic development. Mutations in this gene may be involved in several disorders of the pancreas or in diabetes mellitus. Belongs to the Antp homeobox family. IPF1/XlHbox-8 subfamily. & Contains 1 homeobox DNA-binding domain. Subcellular Location: Nucleus. Tissue Specificity: Duodenum and pancreas.

General readings

Roche TE, Baker JC, Yan X, Hiromasa Y, Gong X, Peng T, Dong J, Turkan A, Kasten SA. Distinct regulatory properties of pyruvate dehydrogenase kinase and phosphatase isoforms Prog Nucleic Acid Res Mol Biol. 2001;70:33-75.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Insulin promoter factor 1 antibody

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PDX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500038) Mouse IgG1 OTI2A12 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Insulin promoter factor 1 antibody

Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-PDX1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807372)(1:150) Mouse IgG2a OTI3H3 Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Insulin promoter factor 1 antibody

Western blot analysis using PDX1 antibody Cat.-No AM06739PU-N against Human PDX1 (AA: 39-283) recombinant protein. (Expected MW is 52 kDa) Mouse IgG1 5A5 Purified Hu E, F, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

Insulin promoter factor 1 antibody

Western blot analysis using PDX1 antibody Cat.-No AM06757PU-N against HEK293 (1) and PDX1 (AA: 39-283)-hIgGFc transfected HEK293 (2) cell lysate. Mouse IgG1 2g12 Purified Hu E, F, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

Insulin promoter factor 1 antibody

Immunohistochemistry-Paraffin: PDX1 Antibody (2A12) [NBP1-47910] - Immunohistochemical staining of paraffin-embedded Carcinoma of pancreas using anti-PDX1 mouse monoclonal antibody. Mouse IgG1 2A12 Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €530.00
  Novus Biologicals Inc.

Insulin promoter factor 1 antibody

TA302585 (1 ug/ml) staining of Human Pancreas lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Chimp, Hu, Ms, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Insulin promoter factor 1 antibody

Predicted band size: 31 kDa . Positive control: Hela and Jurkat cell lysate. Recommended dilution: 1/200-1000 Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Insulin promoter factor 1 antibody

Predicted band size: 31 kDa . Positive control: Hela and Jurkat cell lysate. Recommended dilution: 1/500-2000 Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Insulin promoter factor 1 (C-term) antibody

Ipf Antibody (C-term) (Cat. #TA324981) western blot analysis in U-937 cell line lysates (35ug/lane).This demonstrates the Ipf antibody detected the Ipf protein (arrow). Rabbit Ig Purified Hu WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

Insulin promoter factor 1 antibody

Western blot analysis of extracts of various cell lines; using PDX1antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Insulin promoter factor 1 antibody

WB Suggested Anti-PDX1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: PANC1 cell lysatePDX1 is strongly supported by BioGPS gene expression data to be expressed in Human PANC1 cells Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

Insulin promoter factor 1 antibody

Western Blot: PDX1 Antibody [TA336928] - WB analysis of PDX1 in INS1 cell lysate. Rabbit Purified Hu, Ms C, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Insulin promoter factor 1 antibody

Host: Rabbit; Target Name: Pdx1; Sample Tissue: Rat Thymus lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Sh, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

Insulin promoter factor 1 antibody

Insulin promoter factor 1 Mouse IgG1 OTI5D5 Purified Hu ICC/IF, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

Insulin promoter factor 1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDX1 (RC222354, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDX1. Mouse IgG1 OTI1E1 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Insulin promoter factor 1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDX1 (RC222354, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDX1. Mouse IgG1 OTI4D8 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Insulin promoter factor 1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDX1 (RC222354, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDX1.(1:2000) Mouse IgG1 OTI4H7 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Insulin promoter factor 1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDX1 (RC222354, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDX1.(1:2000) Mouse IgG1 OTI7F7 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Insulin promoter factor 1 pThr11 antibody

Dot blot analysis of anti-Phospho-IPF-pT11 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml. Rabbit Ig Aff - Purified Hu
0.4 ml / €370.00
  Acris Antibodies GmbH

Insulin promoter factor 1 (N-term) antibody

Formalin-fixed and paraffin-embedded human testis tissue reacted with PDX1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Aff - Purified Hu P
0.4 ml / €370.00
  Acris Antibodies GmbH

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