Integrin-linked protein kinase antibody

Principal name

Integrin-linked protein kinase antibody

Alternative names for Integrin-linked protein kinase antibody

ILK-1, ILK-2, p59ILK, ILK, ILK1, ILK2, 59 kDa serine/threonine-protein kinase

SwissProt ID

O55222 (Mouse), P57044 (Cavpo), Q13418 (Human), Q3SWY2 (Bovin), Q6PHD6, Q99J82 (Rat)

Gene ID

3611 (ILK), 16202 (Ilk), 4393543

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Ze (Zebrafish)

Available hosts

Rabbit, Mouse, Goat

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Dot blot (Dot), Frozen Sections (C), Flow Cytometry (F)

Background of Integrin-linked protein kinase antibody

The cellular interactions with extracellular matrix regulate cell shape, growth, survival, morphology and differentiation by regulation various genes through integrin-mediated signal transduction. Integrin-linked protein kinase (ILK) is a ubiquitously expressed protein kinase that has emerged as one of the points of convergence between integrin- and growth factor-signaling pathways. The ILK a member of this pathway and interacts with beta 1-integrin cytoplasmic domain. ILK 1 is a 59 kDa serine/threonine protein kinase containing 4 ankyrin-like repeat. The ILK1 phosphorylates beta 1-integrin cytoplasmic domain and co-immunoprecipitate with beta 1-integrin form cell lysates (1). Over expression of ILK1 disrupted epithelial cell architecture and inhibited the cells to adhere to integrin substrates subsequently reducing the anchorage-independent growth (1). The ILK1 is a receptor-proximal protein kinase regulating integrin-mediated signal transduction cascade. ILK1 is considered to be one of the convergence point of integrin- and growth factor-signaling pathway and is involved in maintenance of cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells.
AKT1 phosphorylates beta 1 and beta 3 integrin subunit on serine and threonine residues and also the AKT1 and GSK3B proteins. ILK1 is stimulated rapidly but transiently by both cell fibronectin interactions, as well as by insulin, in a PI3-K-dependent manner, likely via the binding of PtdIns(3,4,5)P3 with a PH-like domain of ILK1. ILK1 is highly expressed in heart followed by skeletal muscle, pancreas and kidney and is weakly expressed in placenta, lung and liver. ILK1/2 undergoes autophosphorylation on serine residue. ILK1 is considered to be one of the convergence points of integrin- and growth factor-signaling pathway and is involved in maintenance of cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. The growth factor modulation of expression of ILK depends upon the internalization/recycling of the growth factor receptors and stimulation of PI3K pathways. These steps have direct implications with regards to cell adhesion and tumourogenesis.

General readings

Hannigan GE, Leung-Hagesteijn C, Fitz-Gibbon L, Coppolino MG, Radeva G, Filmus J, Bell JC, Dedhar S. Regulation of cell adhesion and anchorage-dependent growth by a new beta 1-integrin-linked protein kinase. Nature. 1996 Jan 4;379(6560):91-6. PMID: 8538749

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Integrin-linked protein kinase antibody

Western Blot analysis of ILK expression in transfected 293T cell line by ILK monoclonal antibody (M01), clone 4F10.

Lane 1: ILK transfected lysate(51.4 KDa).
Lane 2: Non-transfected lysate. Mouse IgG1 4F10 Purified Hu, Ms, Rt E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Integrin-linked protein kinase antibody

Western blot analysis of extracts from rat liver cells, using ILK (Ab-246) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Integrin-linked protein kinase antibody

Western blot analysis of extracts of various cell lines, using ILK antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Integrin-linked protein kinase antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ILK (RC201544, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ILK. Mouse IgG2b OTI2H3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Integrin-linked protein kinase antibody

Western blot analysis of ILK1 expression in 3T3 cell lysate. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €370.00
  Acris Antibodies GmbH

Integrin-linked protein kinase Control Peptide

Integrin-linked protein kinase
0.25 mg / €250.00
  Acris Antibodies GmbH

Integrin-linked protein kinase (C-term) antibody

Integrin-linked protein kinase Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €420.00
  Acris Antibodies GmbH

Integrin-linked protein kinase pThr173 antibody

Dot blot analysis of anti-ILK-pThr173 Pab (Cat#AP12903PU-N) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml. Rabbit Ig Aff - Purified Hu
0.4 ml / €370.00
  Acris Antibodies GmbH

Integrin-linked protein kinase (C-term) antibody

(LEFT) Western blot analysis of anti-ILK2/ILK1 Antibody (C-term) in Hela cell line lysates (35ug/lane). ILK2(arrow) was detected using the purified Pab. (RIGHT) Western blot analysis of anti-ILK2/ILK1 Antibody (C-term) in mouse heart tissue lysates (35ug/lane). ILK2(arrow) was detected using the purified Pab. Rabbit Ig Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Integrin-linked protein kinase (N-term) antibody

(LEFT)Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. (RIGHT)Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ILK1/ILK2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Integrin-linked protein kinase antibody

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ILK Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Integrin-linked protein kinase antibody

Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with Phospho ILK Antibody (T173) antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Integrin-linked protein kinase antibody

Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with Phospho ILK Antibody (S343), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Aff - Purified Hu P
0.4 ml / €370.00
  Acris Antibodies GmbH

Integrin-linked protein kinase antibody

Immunohistochemistry (IHC) analyzes of ILK antibody (Cat.-No.: AP21113PU-N) in paraffin-embedded human brain tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Integrin-linked protein kinase pSer246 antibody

Immunohistochemical analysis of paraffin-embedded human brain tissue using ILK (Phospho-Ser246) antibody AP55757PU-N (left)or the same antibody preincubated with blocking peptide (right). Rabbit Aff - Purified Hu, Ms, Rt P
0.1 ml / €340.00
  Acris Antibodies GmbH

Integrin-linked protein kinase pSer246 antibody

Immunohistochemical analysis of paraffin-embedded human brain tissue using ILK (Phospho-Ser246) antibody AP55757PU-N (left)or the same antibody preincubated with blocking peptide (right). Rabbit Aff - Purified Hu, Ms, Rt P
50 µl / €270.00
  Acris Antibodies GmbH

Integrin-linked protein kinase antibody

Integrin-linked protein kinase Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

Integrin-linked protein kinase pThr173 antibody

Dot blot analysis of ILK (phospho T173) polyclonal antibody ( Cat # PAB1640 ) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 µg/ml. Rabbit Hu Dot, E
0.4 ml / €400.00
  Abnova Taiwan Corp.

Integrin-linked protein kinase antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with Ilk polyclonal antibody ( Cat # PAB3057 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. Rabbit Hu E, P, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

Integrin-linked protein kinase pThr173 antibody

Integrin-linked protein kinase Rabbit Hu C, E, WB
0.4 ml / €400.00
  Abnova Taiwan Corp.

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