Interleukin-1 alpha / IL-1A antibody

Principal name

Interleukin-1 alpha / IL-1A antibody

Alternative names for Interleukin-1 alpha / IL-1A antibody

IL-1 alpha, IL1F1, IL1A, Hematopoietin-1

SwissProt ID

O46612 (Canfa), O46613 (Felca), P01582 (Mouse), P01583 (Human), P04822 (Rabit), P08831 (Bovin), P16598 (Rat), P18430 (Pig), Q28385 (Horse), Q28579 (Sheep)

Gene ID

16175 (Il1a), 3552 (IL1A), 24493

Available reactivities

Ms (Mouse), Hu (Human), Rt (Rat), Mky (Monkey), Bov (Bovine)

Available hosts

Goat, Rabbit, Mouse, Sheep, Human, Hamster, Rat

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Functional assay (FN), Paraffin Sections (P), Flow Cytometry (F), Immunoprecipitation (IP), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Neutralisation (NEUT), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of Interleukin-1 alpha / IL-1A antibody

Interleukin 1 (IL-1) is a name that designates two proteins, IL-1. and IL-1., which are the products of distinct genes, but which recognize the same cell surface receptors. With the exception of skin keratinocytes, some epithelial cells, and certain cells of the central nervous system, IL-1 is not produced by the cells of healthy individuals. However, in response to stimuli such as those produced by inflammatory agents, infections, or microbial endotoxins, a dramatic increase in the production of IL-1 by macrophages and various other cell types is seen. For reviews on the properties and activities of IL-1. and IL-1., see references 1 - 3.

IL-1. and IL-1. are structurally related polypeptides that show approximately 25% homology at the amino acid level . Both are synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17.5 kDa . Neither IL-1. nor IL-1.contains a typical hydrophobic signal peptide sequence , but evidence suggests that these factors can be secreted by non-classical pathways . A large proportion of IL-1. is retained intracellularly in its precursor form . A portion of this unprocessed IL-1. is transported to the cell surface and remains associated with the cell membrane . The membrane-bound, unprocessed IL-1. is apparently biologically active, acting in a paracrine fashion on adjacent cells having IL-1 receptors . The precursor form of IL-1., unlike the IL-1. precursor, shows little or no biological activity in comparison to the 17.5 kDa processed form . Intracellular IL-1.consists exclusively of the 31 kDa precursor form . Extracellular IL-1. consists of a mixture of both unprocessed and mature IL-1.. These results indicate that processing takes place subsequent to secretion and is not tightly coupled to secretion . The specific protease apparently responsible for the processing of IL-1., designated interleukin1.-converting enzyme (ICE), has been described .

IL-1. and IL-1. exert their effects by binding to specific receptors. Two distinct receptor types have been isolated that bind both forms of IL-1. An 80 kDa membrane bound receptor protein, IL-1 receptor type I (IL-1 RI), has been isolated from T cells, fibroblasts, keratinocytes, endothelial cells, synovial lining cells, chondrocytes, and hepatocytes . IL-1 RI has been cloned from mouse and human cells and found to be a member of the Ig super family. A second type of IL-1 receptor, IL-1 receptor type II (IL-1 RII), has been found on B cells, neutrophils, and bone marrow cells . This receptor has an apparent molecular weight of about 68 kDa and is also a member of the Ig super family. The two IL-1 receptor types show approximately 28% homology in their extracellular domains, but differ significantly in that the type II receptor has a cytoplasmic domain of only 29 amino acid residues, whereas the type I receptor has a cytoplasmic domain of 213 amino acid residues . In general, IL-1. binds better to the type I receptor and IL-1. binds better to the type II receptor . At present, the mechanisms involved in the transduction of the signal initiated by binding of IL-1 are not well characterized . IL-1 possesses a wide variety of biological activities. It has been shown to induce prostaglandin synthesis in endothelial cells and smooth muscle cells . In the liver, IL-1 initiates the acute phase response resulting in an increase in hepatic protein synthesis and decreased albumin production .

General readings

Arend, W.P. (2002) Cytokine Growth Factor Rev. 13:323.

Sims, J.E. et al. (2001) Trends Immunol. 22:536.

Nickel, W. (2003) Eur. J. Biochem. 270:2109.

Dinarello, C.A. (1991) Blood 77:1627.

Nishida, T. et al. (1989) J. Biochem. 105 :351.

Wessendorf, J.H.M. et al. (1993) J. Biol. Chem. 268:22100.

Beuscher, H.U. et al. (1988) J. Biol. Chem. 263:4023.

Stevenson, F.T. et al. (1993) Proc. Natl. Acad. Sci. USA 90:7245.

Werman, A. et al. (2004) Proc. Natl. Acad. Sci. USA 101:2434.

Pollock, A.S. et al. (2003) FASEB J. 17:203.

Kaplanski, G. et al. (1994) Blood 84:4242.

Brody, D.T. and S.K. Durum (1989) J. Immunol. 143:1183.

Apte, R.N. and E. Voronov (2002) Semin. Cancer Biol. 12:277.

Kobayashi, Y. et al. (1990) Proc. Natl. Acad. Sci. USA 87:5548.

Dinarello, C.A. (1996) Blood 87:2095.

Boutin, H. et al. (2003) Mol. Neurobiol. 27:239.

Lomedico, P.T. et al. (1984) Nature 312:458.

Furutani, Y. et al. (1985) Nucleic Acids Res. 13:5869.

March, C.J. et al. (1985) Nature 315:641.

Leong, S.R. et al. (1988) Nucleic Acids Res. 16:9053.

Straubinger, A.F. et al. (1999) Gene 236 :273.

Burysek, L. and J. Houstek (1996) Cytokine 8:460.

Takei, T. et al. (1998) J. Cell. Biochem. 69:95.

Okubo, Y. and M. Koga (1998) J. Dermatol. Sci. 17:223.

Hernandez-Pando, R. et al. (1997) Immunology 90:607.

Lundberg, I.E. and P. Nyberg (1998) Curr. Opin. Rheumatol. 10:521.

Lin, T-J. et al. (2002) J. Immunol. 169:4522.

Skundric, D.S. et al. (2001) J. Neuroimmunol. 116:74.

Lossdorfer, S. et al. (2002) Cytokine 20:7.

Sims, J.E. (2002) Curr. Opin. Immunol. 14:117.

Martin, M.U. and H. Wesche (2002) Biochim. Biophys. Acta 1592:265.

Vidal, O.N.A. et al. (1998) Biochem. Biophys. Res. Commun. 248:696.

Murphy, J-E. et al. (2000) J. Invest. Dermatol. 114:602.

Ristimaki, A. et al. (1998) J. Bio

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Interleukin-1 alpha / IL-1A antibody

  IL1A mouse monoclonal antibody, clone OTI2F8 (formerly 2F8)  
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-IL1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506849) Mouse IgG1 OTI2F8 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Interleukin-1 alpha / IL-1A antibody

  IL1A mouse monoclonal antibody, clone OTI2F11 (formerly 2F11)  
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-IL1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506850) Mouse IgG1 OTI2F11 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Interleukin-1 alpha / IL-1A antibody

  IL1A mouse monoclonal antibody, clone OTI3C3 (formerly 3C3)  
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-IL1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506853) Mouse IgG1 OTI3C3 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Interleukin-1 alpha / IL-1A antibody

  Mouse Anti-Human IL-1 alpha Purified (50 ug)  
Interleukin-1 alpha / IL-1A Mouse 364/3B3-14 Purified Hu E, F
50 µg / €120.00
  OriGene Technologies, Inc.

Interleukin-1 alpha / IL-1A antibody

  Hamster Anti-Mouse IL-1 alpha Purified (50 ug)  
Mouse splenocytes (500,000 cells per well) were activated with LPS (1 ug/ml) for 48 hrs in mouse IL-1 alpha ELISPOT assay.  Control well is medium alone. Hamster ALF-161 Purified Ms E
50 µg / €120.00
  OriGene Technologies, Inc.

Interleukin-1 alpha / IL-1A antibody

  IL1A Rabbit Polyclonal Antibody  
Western blot analysis of extracts of  various cell lines, using IL1A antibody. Rabbit IgG Purified Hu
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-1 alpha / IL-1A antibody

  Anti-Human IL-1alpha Rabbit Polyclonal Antibody  
This antibody stained formalin-fixed, paraffin-embedded sections of normal human skin.  The recommended concentration is 1.0 ug/ml overnight at 4C.  An HRP-labeled polymer detection system was used with a non-alcohol soluble AEC chromogen.  Enzyme induced antigen retrieval was performed with proteinase K.  Optimal concentrations and conditions may vary. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-1 alpha / IL-1A antibody

  Biotinylated Anti-Murine IL-1a Rabbit Polyclonal Antibody  
To detect mIL-1alpha; by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant mIL-1alpha;. Rabbit IgG Purified E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Interleukin-1 alpha / IL-1A antibody

  Anti-Murine IL-1a Rabbit Polyclonal Antibody  
To detect mIL-1alpha; by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine IL-1alpha; (TA328476) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-1alpha;. Rabbit IgG Purified E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-1 alpha / IL-1A antibody

  Biotinylated Anti-Rat IL-1a Goat Polyclonal Antibody  
To detect Rat IL-1alpha; by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat IL-1alpha;. Goat IgG Purified Rt E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Interleukin-1 alpha / IL-1A antibody

  Anti-Rat IL-1a Goat Polyclonal Antibody  
To detect Rat IL-1alpha; by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Rat IL-1alpha; (TA328562) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-1alpha;. Goat IgG Purified Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-1 alpha / IL-1A antibody

  Rat Monoclonal anti-IL-1alpha Antibody  
Interleukin-1 alpha / IL-1A Rat BL1a-72 Purified Ms E
0.5 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-1 alpha / IL-1A antibody

  Mouse Monoclonal anti-IL-1alpha Antibody  
Interleukin-1 alpha / IL-1A Mouse 364-3B3-14 Purified Hu E, P
0.5 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-1 alpha / IL-1A antibody

  IL1A mouse monoclonal antibody, clone OTI1G2 (formerly 1G2)  
Anti-IL1A mouse monoclonal antibody (TA506846) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY IL1A(RC202084). Mouse IgG1 OTI1G2 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Interleukin-1 alpha / IL-1A antibody

  IL1A mouse monoclonal antibody, clone OTI2D3 (formerly 2D3)  
Anti-IL1A mouse monoclonal antibody (TA506847) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY IL1A(RC202084). Mouse IgG1 OTI2D3 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Interleukin-1 alpha / IL-1A antibody

  IL1A mouse monoclonal antibody, clone OTI2E7 (formerly 2E7)  
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-IL1A mouse monoclonal antibody (TA506848) at 1:150 dilution. Mouse IgG1 OTI2E7 Purified Hu ICC/IF, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Interleukin-1 alpha / IL-1A antibody

  IL1A mouse monoclonal antibody, clone OTI2B4 (formerly 2B4)  
Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY IL1A(RC202084) using Anti-IL1A mouse monoclonal antibody (TA506851) at 1:100 dilution. Mouse IgG1 OTI2B4 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Interleukin-1 alpha / IL-1A antibody

  IL1A mouse monoclonal antibody, clone OTI3A5 (formerly 3A5)  
Anti-IL1A mouse monoclonal antibody (TA506852) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY IL1A(RC202084). Mouse IgG1 OTI3A5 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Interleukin-1 alpha / IL-1A antibody

  Rabbit Polyclonal IL1 alpha Antibody  
ELISA: IL1 alpha Antibody Rabbit IgG Purified Hu E
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-1 alpha / IL-1A antibody

Interleukin-1 alpha / IL-1A Mouse IgG1 AS5 FITC Hu F
100 Tests / €370.00
  Acris Antibodies GmbH

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