Interleukin-1 beta / IL-1B antibody

Principal name

Interleukin-1 beta / IL-1B antibody

Alternative names for Interleukin-1 beta / IL-1B antibody

IL-1 beta, IL1B, IL1 beta, IL1F2, Catabolin

SwissProt ID

P01584 (Human), P09428 (Bovin), P10749 (Mouse), P14628 (Rabit), P21621 (Sheep), P26889 (Pig), P41687 (Felca), Q28292 (Canfa), Q28386 (Horse), Q63264 (Rat)

Gene ID

3553 (IL1B), 16176 (Il1b), 281251, 397122, 443539 (IL-1B), 24494

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey), Por (Porcine), Bov (Bovine), Gt (Goat), Sh (Sheep), Chk (Chicken), Can (Canine)

Available hosts

Mouse, Goat, Rabbit, Sheep, Hamster

Available applications

Enzyme Immunoassay (E), ELISA (capture) (E(capture)), ELISA (detection) (E(detection)), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Functional assay (FN), Paraffin Sections (P), Flow Cytometry (F), Immunoprecipitation (IP), Neutralisation (NEUT), EPair for Elisa (EPair), Radioimmunoassay (R)

Background of Interleukin-1 beta / IL-1B antibody

Interleukin-1 (IL-1), originally described as lymphocyte activating factor (LAF) for its effects on thymocytes, is a polypeptide cytokine with two molecular forms. The two distinct molecular forms of IL-1 are thought to be derived from two genes. After transcription, as 31kD precursor polypeptide is cleaved to give rise to mostly cell membrane associated IL-1. and secreted IL-1.. Both have the same molecular weight of 15kD but have different isoelectric points of 5 and 7, respectively.

Despite sequence homology of only 20%, both forms are thought to bind to the same receptor. IL-1 inhibitors that vary only in their degree of glycosylation have been described to bind to the IL-1 receptor. These inhibitors are structurally related to IL-1. and may be important in regulation of IL-1. action.

IL-1. is produced primarily by monocytes and macrophages but also by astrocytes, oligodendroglia, adrenal cortical cells, NK cells, endothelial cells, keratinocytes, megakaryocytes, platelets, neurons, neutrophils, osteoblasts, Schwann cells, trophoblasts, T cells, and fibroblasts. IL-1 has multiple immunological functions including enhancement of IL-2 production by T cells and activation of B-cells (BAF) and thymocytes. A true pleiotrope, IL-1 may have tumoricidal activity via its release of IL-2 and Interferon gamma and indirectly antiviral by stimulating to release interferon beta.

Low levels of IL-1. have been reported in normal serum. It is thought that IL-1 genes are induced to respond to tissue damage or in infection. Elevated levels have been reported in a number of infectious disease conditions and in noninfectious inflammatory conditions such as CrohnÂ?s disease. In addition to elevated serum levels, IL-1 has been found in synovial fluids of patients with rheumatoid arthritis and in cerebrospinal fluid after neurological inflammation or insult. At the other end of the spectrum, low levels of IL-1 have been found in malnutrition and advanced neoplasia suggesting perhaps a complex immunological and physiological regulatory role for this cytokine.

General readings

Dinarello, C.A. (1991) Blood 77:1627.

Oppenheim, J.J. et al. (1986) Immunol. Today 7:45.

Dinarello, C.A. and S.M. Wolff (1993) New Engl. J. Med. 328:106.

Giri, J.G. et al. (1985) J. Immunol. 134:343.

Hazuda, D.J. et al. (1988) J. Biol. Chem. 263:8473.

Kurt-Jones, E.A. et al. (1985) Proc. Natl. Acad. Sci. USA 82:1204.

15. Cerretti, D.P. et al. (1992) Science 256:97.USA 86:5943.

Levett, D. et al. (1986) J. Immunol. 136:340.

Navarro, S. et al. (1989) J. Immunol. 142:4339.

Kaye, J. et al. (1984) Lymphokine Res. 3:175.

Herrmann, F. et al. (1988) J. Clin. Invest. 81:1415.

Abbas, A.K. et al. (1987) Am. J. Pathol. 129:26.

Lipsky, P.E. et al. (1983) J. Immunol. 130:2709.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Interleukin-1 beta / IL-1B antibody

Figure from citation: Immunohistochemistry of IL1B protein level by using anti-IL1B antibody in synovial tissues from fracture and OA patients, respectively. Dilution: 1:100 View Citation Mouse IgG1 OTI3E1 Purified Hu ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

Interleukin-1 beta / IL-1B antibody

Interleukin-1 beta / IL-1B Goat IgG Aff - Purified Rt C, E(capture), NEUT, WB
0.1 mg / €450.00
  Neuromics Antibodies

Interleukin-1 beta / IL-1B antibody

Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-IL1B rabbit polyclonal antibody. (TA590024) Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-1 beta / IL-1B antibody

Immunohistochemical analysis of paraffin-embedded muscle tissues using IL1B mouse mAb with DAB staining. Mouse IgG1 3A6 Ascites Hu E, ICC/IF, P, WB
0.1 ml / €430.00
  Acris Antibodies GmbH

Interleukin-1 beta / IL-1B antibody

IL-1B was used at a 1:200 dilution incubated 1 h at RT to detect dog IL-1B by WB. Lane 1 and 2 were loaded with 2.5 ug and 500 ng of dog IL-1B respectively. The molecular weight of the detected band is estimated by comparison to molecular weight markers (not shown). Detection occurred using a 1:3,000 dilution of IRDye™800 conjugated Donkey anti-Rabbit IgG. Rabbit Purified Can, Hu, Mky C, E, FN, IP, P, R, WB
1 mg / €900.00
  OriGene Technologies, Inc.

Interleukin-1 beta / IL-1B antibody

This antibody will recognize 10% of the non-denatured (native) precursor 31,000 MW mouse IL-1ß containing samples but will primarily detect all of the 17,000 MW mature molecule. However, in western blot analysis, the usual procedure of heating the sample in SDS with or without reducing agents will facilitate denaturing of the 31,000 MW IL- 1ß precursor molecule. Denatured IL-1ß will have a 18 kDa band. Rabbit Azide Free Ms, Rt C, E, ICC/IF, IP, NEUT, P, R, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-1 beta / IL-1B antibody

Western Blot of Rabbit anti-Mouse IL-1 Beta Antibody Peroxidase Conjugated Lane 1: Mouse IL-1 Beta Load: 50 ng per lane Secondary antibody: IL1 beta Antibody Peroxidase Conjugated at 1:1,000 for 30 min at RT Block: MB-070 for 30 min RT Predicted/Observed size: 18 kDa, 18 kDa Rabbit IgG Purified Ms E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-1 beta / IL-1B antibody

Interleukin-1 beta / IL-1B Hamster B122 Purified Ms, Rt E
50 µg / €120.00
  OriGene Technologies, Inc.

Interleukin-1 beta / IL-1B antibody

Staining of human peripheral blood mononuclear cells stimulated with LPS in the presence of Brefeldin A for 4 hours with Anti-Human CD14 PE (left) or Anti-Human CD14 FITC (right) followed by intracellularly Anti-Human IL-1 beta PE. Mouse CRM56 Purified Hu F
50 µg / €120.00
  OriGene Technologies, Inc.

Interleukin-1 beta / IL-1B antibody

Predicted cell location: Cytoplasm . Positive control: Human lymphoma and lung cancer tissue . Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human lymphoma tissue using IL1B antibody at dilution 1/20, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Interleukin-1 beta / IL-1B antibody

Western blot analysis of human adrenal cell lysate using IL1B antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-1 beta / IL-1B antibody

To detect mIL-1beta by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant mIL-1beta. Rabbit IgG Purified E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Interleukin-1 beta / IL-1B antibody

To detect mIL-1beta by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine IL-1beta (TA328478) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-1beta. Rabbit IgG Purified E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-1 beta / IL-1B antibody

To detect Rat IL-1beta by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat IL-1beta. Rabbit IgG Purified Rt E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Interleukin-1 beta / IL-1B antibody

To detect Rat IL-1beta by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Rat IL-1beta (TA328564) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-1beta. Rabbit IgG Purified Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-1 beta / IL-1B antibody

TA334185 (1µg/ml) staining of Human Peripheral Blood Lymphocytes lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-1 beta / IL-1B antibody

Western Blot: IL1 beta Antibody [TA336742] - Western blot (WB) analysis of IL1 beta Antibody in extracts from HeLa cells. Rabbit Purified Hu, Ms, Rt C, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-1 beta / IL-1B antibody

Western blot analysis of IL-1 beta expression in HeLa cell lysate Rabbit IgG Purified Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-1 beta / IL-1B antibody

Interleukin-1 beta / IL-1B Mouse IgG1 B-A15 Purified Hu F
0.5 mg / €413.00
  OriGene Technologies, Inc.

Interleukin-1 beta / IL-1B antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL1B (RC202079, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL1B. Mouse IgG1 OTI1A7 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

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