Interleukin-1 beta / IL-1B antibody

Principal name

Interleukin-1 beta / IL-1B antibody

Alternative names for Interleukin-1 beta / IL-1B antibody

IL-1 beta, IL1B, IL1 beta, IL1F2, Catabolin

SwissProt ID

P01584 (Human), P09428 (Bovin), P10749 (Mouse), P14628 (Rabit), P21621 (Sheep), P26889 (Pig), P41687 (Felca), Q28292 (Canfa), Q28386 (Horse), Q63264 (Rat)

Gene ID

3553 (IL1B), 16176 (Il1b), 281251, 397122, 443539 (IL-1B), 24494

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey), Por (Porcine), Bov (Bovine), Gt (Goat), Sh (Sheep), Chk (Chicken), Bab (Baboon), Can (Canine)

Available hosts

Mouse, Goat, Rabbit, Sheep, Hamster

Available applications

Enzyme Immunoassay (E), ELISA (capture) (E(capture)), ELISA (detection) (E(detection)), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Functional assay (FN), Paraffin Sections (P), Flow Cytometry (F), Immunoprecipitation (IP), Neutralisation (NEUT), EPair for Elisa (EPair), Radioimmunoassay (R)

Background of Interleukin-1 beta / IL-1B antibody

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE).Two forms of interleukin-1, desigted IL-1alpha and IL-1beta, have been described. Although encoded by distinct genes and exhibiting roughly only 25% sequence identity, IL-1alpha and IL-1beta bind to the same receptor and seem to elicit similar biological responses. This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygese-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. In T cells, IL-1 stimulates the production of IL-2 and selectively inhibits IL-4 expression and it induces B cell proliferation, maturation and immunoglobulin synthesis. NK cells require IL-1beta for production of the anti- pathogen IFN-gamma. IL-1 has also been implicated in several pathological conditions including rheumatoid arthritis, inflammatory bowel disease and atherosclerosis.

General readings

General / background references:Cerretti DP et al. Molecular cloning of the interleukin-1 beta converting enzyme. Science 256:97-100 (1992). PubMed PMID: 1373520
Herzyk DJ et al. Sandwich ELISA formats designed to detect 17 kDa IL-1 beta significantly underestimate 35 kDa IL-1 beta. J Immunol Methods 148:243-54 (1992). PubMed PMID: 1564329
Van Damme J et al. Homogeneous interferon-inducing 22K factor is related to endogenous pyrogen and interleukin-1. Nature 314:266-8 (1985). PubMed PMID: 3920526
Auron PE et al. Nucleotide sequence of human monocyte interleukin 1 precursor cDNA. Proc Natl Acad Sci U S A 81:7907-11 (1984). PubMed PMID: 6083565

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Interleukin-1 beta / IL-1B antibody

Figure from citation: Immunohistochemistry of IL1B protein level by using anti-IL1B antibody in synovial tissues from fracture and OA patients, respectively. Dilution: 1:100 View Citation Mouse IgG1 OTI3E1 Purified Hu ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

Interleukin-1 beta / IL-1B antibody

Interleukin-1 beta / IL-1B Goat IgG Aff - Purified Rt C, E(capture), NEUT, WB
0.1 mg / €450.00
  Neuromics Antibodies

Interleukin-1 beta / IL-1B antibody

Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-IL1B rabbit polyclonal antibody. (TA590024) Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-1 beta / IL-1B antibody

Immunohistochemical analysis of paraffin-embedded muscle tissues using IL1B mouse mAb with DAB staining. Mouse IgG1 3A6 Ascites Hu E, ICC/IF, P, WB
0.1 ml / €430.00
  Acris Antibodies GmbH

Interleukin-1 beta / IL-1B antibody

IL-1B was used at a 1:200 dilution incubated 1 h at RT to detect dog IL-1B by WB. Lane 1 and 2 were loaded with 2.5 ug and 500 ng of dog IL-1B respectively. The molecular weight of the detected band is estimated by comparison to molecular weight markers (not shown). Detection occurred using a 1:3,000 dilution of IRDye™800 conjugated Donkey anti-Rabbit IgG. Rabbit Purified Can, Hu, Mky C, E, FN, IP, P, R, WB
1 mg / €900.00
  OriGene Technologies, Inc.

Interleukin-1 beta / IL-1B antibody

This antibody will recognize 10% of the non-denatured (native) precursor 31,000 MW mouse IL-1ß containing samples but will primarily detect all of the 17,000 MW mature molecule. However, in western blot analysis, the usual procedure of heating the sample in SDS with or without reducing agents will facilitate denaturing of the 31,000 MW IL- 1ß precursor molecule. Denatured IL-1ß will have a 18 kDa band. Rabbit Azide Free Ms, Rt C, E, ICC/IF, IP, NEUT, P, R, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-1 beta / IL-1B antibody

Western Blot of Rabbit anti-Mouse IL-1 Beta Antibody Peroxidase Conjugated Lane 1: Mouse IL-1 Beta Load: 50 ng per lane Secondary antibody: IL1 beta Antibody Peroxidase Conjugated at 1:1,000 for 30 min at RT Block: MB-070 for 30 min RT Predicted/Observed size: 18 kDa, 18 kDa Rabbit IgG Purified Ms E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-1 beta / IL-1B antibody

Interleukin-1 beta / IL-1B Hamster B122 Purified Ms, Rt E
50 µg / €120.00
  OriGene Technologies, Inc.

Interleukin-1 beta / IL-1B antibody

Staining of human peripheral blood mononuclear cells stimulated with LPS in the presence of Brefeldin A for 4 hours with Anti-Human CD14 PE (left) or Anti-Human CD14 FITC (right) followed by intracellularly Anti-Human IL-1 beta PE. Mouse CRM56 Purified Hu F
50 µg / €120.00
  OriGene Technologies, Inc.

Interleukin-1 beta / IL-1B antibody

Predicted cell location: Cytoplasm . Positive control: Human lymphoma and lung cancer tissue . Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human lymphoma tissue using IL1B antibody at dilution 1/20, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Interleukin-1 beta / IL-1B antibody

Western blot analysis of human adrenal cell lysate using IL1B antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-1 beta / IL-1B antibody

To detect mIL-1beta by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant mIL-1beta. Rabbit IgG Purified E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Interleukin-1 beta / IL-1B antibody

To detect mIL-1beta by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine IL-1beta (TA328478) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-1beta. Rabbit IgG Purified E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-1 beta / IL-1B antibody

To detect Rat IL-1beta by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat IL-1beta. Rabbit IgG Purified Rt E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Interleukin-1 beta / IL-1B antibody

To detect Rat IL-1beta by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Rat IL-1beta (TA328564) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-1beta. Rabbit IgG Purified Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-1 beta / IL-1B antibody

TA334185 (1µg/ml) staining of Human Peripheral Blood Lymphocytes lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-1 beta / IL-1B antibody

Western Blot: IL1 beta Antibody [TA336742] - Western blot (WB) analysis of IL1 beta Antibody in extracts from HeLa cells. Rabbit Purified Hu, Ms, Rt C, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-1 beta / IL-1B antibody

Western blot analysis of IL-1 beta expression in HeLa cell lysate Rabbit IgG Purified Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-1 beta / IL-1B antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL1B (RC202079, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL1B. Mouse IgG1 OTI1A7 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Interleukin-1 beta / IL-1B antibody

Interleukin-1 beta / IL-1B Mouse IgG1 AS10 FITC Hu F
100 Tests / €360.00
  Acris Antibodies GmbH

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