Interleukin-10 / IL10 antibody

Principal name

Interleukin-10 / IL10 antibody

Alternative names for Interleukin-10 / IL10 antibody

IL-10, CSIF, TGIF, Cytokine synthesis inhibitory factor

SwissProt ID

P18893 (Mouse), P22301 (Human), P29456 (Rat), P43480 (Bovin), P48411 (Canfa), P55029 (Felca), Q28374 (Horse), Q29055 (Pig), Q29408 (Sheep), Q6A2H4 (Chick), Q9TSJ4 (Rabit), Q9Z1Y5 (Cavpo)

Gene ID

3586 (IL10), 16153 (Il10), 25325, 281246

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Eq (Equine), Gt (Goat), Sh (Sheep), Mky (Monkey), Can (Canine), Chk (Chicken), Rb (Rabbit)

Available hosts

Mouse, Rabbit, Rat, Goat, Chicken, Sheep

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Functional assay (FN), Frozen Sections (C), Immunoprecipitation (IP), Radioimmunoassay (R), Flow Cytometry (F), ELISA (detection) (E(detection)), Neutralisation (NEUT), ELISA (capture) (E(capture)), Immunocytochemistry/Immunofluorescence (ICC/IF), Luminex (LMNX)

Background of Interleukin-10 / IL10 antibody

Interleukin-10 is a pleiotropic cytokine playing an important role as a regulator of lymphoid and myeloid cell function. In humans IL-10 is produced by activated CD8(+) peripheral blood T-cells, by T-helper CD4(+) T-cell clones. Due to the ability of IL-10 to block cytokine synthesis and several accessory cell functions of macrophages this cytokine is a potent suppressor of the effector functions of macrophages, T-cells and NK cells. In addition, IL-10 participates in regulating proliferation and differentiation of B-cells, mast cells and thymocytes. The primary structure of human IL-10 has been determined by cloning the cDNA encoding the cytokine. The corresponding protein exerts 160 amino acids with a predicted molecular mass of 18.5 kDa. Based on its primary structure, IL-10 is a member of the four a-helix bundle family of cytokines. In solution human IL-10 is a homodimer with an apparent molecular mass of 39 kDa. Although it contains an N-linked, glycosylation site, it lacks detectable carbohydrates. Recombinant protein expressed in E. coli thus retains all known biological activities. The human IL-10 gene is located on chromosome 1 and is present as a single copy in the genome. The human IL-10 exhibits strong DNA and amino acid sequence homology to the murine IL-10 and an open reading frame in the Epstein-Barr virus genome, BCRF1 which shares many of the cellular cytokine's biological activities and may therefore play a role in the host-virus interaction. The immunosuppressive properties of IL-10 suggest a possible clinical use of IL-10 in suppressing rejections of grafts after organ transplantations. IL-10 can furthermore exert strong anti-inflammatory activities.

General readings

1. Chakravarti, A. Normal human primary CD4+ T lymphocytes synthesize and release functional osteoprotegerin in vitro. Laboratory Investigation.;88(2):171-84. 2/1/2008 18040268
Ward FJ, Clonal regulatory T cells specific for a red blood cell autoantigen in human autoimmune hemolytic anemia Blood, 2007, 111(2):680-7.
2. Darce, J.R. Regulated Expression of BAFF-Binding Receptors during Human B Cell Differentiation. The Journal of Immunology, 2007, 179(11):7276-86.
3. Tran DQ Induction of FOXP3 expression in naive human CD4+FOXP3 T cells by T-cell receptor stimulation is transforming growth factor-beta dependent but does not confer a regulatory phenotype Blood, 2007, 110(8):2983-90.
4. Sakuishi, K. Invariant NKT cells biased for IL-5 production act as crucial regulators of inflammation. The Journal of Immunology, 2007, 179(6):3452-62.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Interleukin-10 / IL10 (Center) antibody

Human breast carcinoma (formalin-fixed, paraffin-embedded) reacted with IL10 antibody Cat.-No. AP52181PU-N, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for IHC; clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu F, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Interleukin-10 / IL10 antibody

This antibody stained formalin-fixed paraffin-embedded sections of human pancreas adenocarcinoma tissue. The recommended concentration is 5.0 µg/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results were achieved without antigen retrieval. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. Mouse IgG1 Azide Free Hu E, P, WB
0.5 mg / €190.00
  Acris Antibodies GmbH

Interleukin-10 / IL10 antibody

Anti-IL-10 antibody IHC of mouse small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. Rat IgG1 JES5-2A5 Purified Ms C, E, F
0.1 mg / €345.00
  OriGene Technologies, Inc.

Interleukin-10 / IL10 antibody

Anti IL-10 whole antiserum (TA319291) was tested against recombinant IL10. Recombinant IL-10 was run on a 4-20% reducing gel, and transferred to nitrocellulose. Membrane was blocked in 3% BSA-TBS for 1 Hour 4oC and probed with p/n TA319291 diluted 1:200 in 3% BSA-TBS o/n 4oC. Primary antibody was detected with Gt anti Rb 611-103-122 Lot#21231 1:20,000 in MB-070 1 Hour 4oC using FemtoMax Super sensitive chemiluminescent substrate. Rabbit Serum Hu C, E, FN, IP, R, WB
1 ml / €900.00
  OriGene Technologies, Inc.

Interleukin-10 / IL10 antibody

To detect hIL-10 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-10. Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Interleukin-10 / IL10 antibody

To detect hIL-10 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-10 (TA328224) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-10. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-10 / IL10 antibody

To detect mIL-10 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant mIL-10. Rabbit IgG Purified E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Interleukin-10 / IL10 antibody

To detect mIL-10 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine IL-10 (TA328494) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-10. Rabbit IgG Purified E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-10 / IL10 antibody

lL-10 ELISA with 9D7 Capture (TA600017) and 12G8 Detection (TA700017) Antibodies. Substrate used: Recombinant Human lL-10 (E. Coli expressed Peptide) Rat IgG1 OTI9D7 Purified Hu E, LMNX
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-10 / IL10 antibody

lL-10 ELISA with 9D7 Capture (TA600017) and 12G8 Detection (TA700017) Antibodies. Substrate used: Recombinant Human lL-10 (E. Coli expressed Peptide) Rat IgG1 OTI12G8 Purified Hu E, LMNX
50 µg / €345.00
  OriGene Technologies, Inc.

Interleukin-10 / IL10 antibody

Interleukin-10 / IL10 Rat IgG1 JES3-9D7 Azide Free Hu E, F, FN
1 mg / €780.00
  Acris Antibodies GmbH

Interleukin-10 / IL10 antibody

Interleukin-10 / IL10 Rat IgG1 JES3-9D7 Azide Free Hu E, F, FN
0.5 mg / €560.00
  Acris Antibodies GmbH

Interleukin-10 / IL10 antibody

Interleukin-10 / IL10 Mouse IgG1 945A5A10 Biotin Hu E(detection)
0.1 mg / €550.00
  Acris Antibodies GmbH

Interleukin-10 / IL10 antibody

Interleukin-10 / IL10 Rat IgG1 JES5-2A5 Azide Free Ms E, FN
0.5 mg / €680.00
  Acris Antibodies GmbH

Interleukin-10 / IL10 antibody

Interleukin-10 / IL10 Rat IgG1 JES5-2A5 Purified Ms E, FN
0.1 mg / €500.00
  Acris Antibodies GmbH

Interleukin-10 / IL10 antibody

Interleukin-10 / IL10 Mouse IgG1 945A5D11 Purified Hu E
0.5 mg / €550.00
  Acris Antibodies GmbH

Interleukin-10 / IL10 antibody

Interleukin-10 / IL10 Mouse IgG2b CC318 Purified Bov, Eq, Sh E, E(capture), Elispot, F
0.5 mg / €670.00
  Acris Antibodies GmbH

Interleukin-10 / IL10 antibody

Western blot analysis using IL10 antibody Cat.-No AM06081PU-N against IL10 recombinant protein. Mouse IgG1 3C12C12 Purified Hu E, WB
0.1 ml / €300.00
  Acris Antibodies GmbH

Interleukin-10 / IL10 antibody

Interleukin-10 / IL10 Mouse IgG2b B-T10 Biotin Hu E(detection), Elispot
0.1 mg / €460.00
  Acris Antibodies GmbH

Interleukin-10 / IL10 antibody

Interleukin-10 / IL10 Mouse IgG1 B-S10 Azide Free Hu E(capture), FN
0.5 mg / €460.00
  Acris Antibodies GmbH

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