Interleukin-10 / IL10 antibody

Principal name

Interleukin-10 / IL10 antibody

Alternative names for Interleukin-10 / IL10 antibody

IL-10, CSIF, TGIF, Cytokine synthesis inhibitory factor

SwissProt ID

P18893 (Mouse), P22301 (Human), P29456 (Rat), P43480 (Bovin), P48411 (Canfa), P55029 (Felca), Q28374 (Horse), Q29055 (Pig), Q29408 (Sheep), Q6A2H4 (Chick), Q9TSJ4 (Rabit), Q9Z1Y5 (Cavpo)

Gene ID

16153 (Il10), 25325, 3586 (IL10), 281246

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Eq (Equine), Gt (Goat), Sh (Sheep)

Available hosts

Mouse, Rabbit, Rat, Sheep

Available applications

Paraffin Sections (P), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Functional assay (FN), Flow Cytometry (F), ELISA (detection) (E(detection)), ELISA (capture) (E(capture)), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Luminex (LMNX), Radioimmunoassay (R)

Background of Interleukin-10 / IL10 antibody

Interleukin 10 (IL-10), initially designated cytokine synthesis inhibitory factor (CSIF), was originally identified as a product of murine T helper 2 (TH2) clones that suppressed the production of cytokines by T helper 1 (TH1) clones responding to antigen stimulation in the presence of monocyte/macrophage antigen-presenting cells .

The human homologue of mouse IL-10 was subsequently cloned by cross-hybridization . Human and mouse IL-10 are 81% and 73% identical at the nucleotide and amino acid levels, respectively. Mouse, rat and human IL-10 cDNAs encode 178 amino acid (aa) residue precursor polypeptides with hydrophobic signal peptides that are cleaved to generate 160 aa residue mature proteins. There is at least 73% aa identity between the proteins from the three species. In contrast to mouse IL-10, human IL-10 appears to be non-glycosylated. Both mouse and human IL-10 exist as non-covalently-linked homodimers in solution. Although human IL-10 is active on mouse cells, mouse IL-10 does not show species cross-reactivity on human cells.

IL-10 is a pleiotropic cytokine that can exert either immunosuppressive or immunostimulatory effects on a variety of cell types. human IL-10 can inhibit monocyte/macrophage-dependent, antigen-stimulated cytokine synthesis by human PBMNC and NK cells . L-10 can inhibit production or function of a macrophage membrane-bound costimulator required for activation of T cells and NK cells.

IL-10 is a potent modulator of monocyte/macrophage function. IL-10 also enhances the release of soluble TNF receptors and inhibits the expression of surface ICAM-1 and B7 . Finally, IL-10 has been reported to suppress the synthesis of superoxide anion plus reactive oxygen intermediates (ROI), and either inhibit or facilitate nitric oxide synthesis, depending on the time of exposure to activated macrophages . IL-10 has marked effects on B cells. IL-10 also has documented activity on endothelial cells, where it mimics IL-4 , and on thymocytes and mast cells, where it acts as a growth costimulator .

General readings

1. Chakravarti, A. Normal human primary CD4+ T lymphocytes synthesize and release functional osteoprotegerin in vitro. Laboratory Investigation.;88(2):171-84. 2/1/2008 18040268
Ward FJ, Clonal regulatory T cells specific for a red blood cell autoantigen in human autoimmune hemolytic anemia Blood, 2007, 111(2):680-7.
2. Darce, J.R. Regulated Expression of BAFF-Binding Receptors during Human B Cell Differentiation. The Journal of Immunology, 2007, 179(11):7276-86.
3. Tran DQ Induction of FOXP3 expression in naive human CD4+FOXP3 T cells by T-cell receptor stimulation is transforming growth factor-beta dependent but does not confer a regulatory phenotype Blood, 2007, 110(8):2983-90.
4. Sakuishi, K. Invariant NKT cells biased for IL-5 production act as crucial regulators of inflammation. The Journal of Immunology, 2007, 179(6):3452-62.

  1. Quick-Filters:
  2. Cited (3)
  3. Highly Validated (1)

Items 1 to 20 of 164 total

per page
Page:
  1. 1
  2. 2
  3. 3
  4. 4
  5. 5

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Interleukin-10 / IL10 (Center) antibody

Human breast carcinoma (formalin-fixed, paraffin-embedded) reacted with IL10 antibody Cat.-No. AP52181PU-N, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for IHC; clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu F, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Interleukin-10 / IL10 antibody

This antibody stained formalin-fixed paraffin-embedded sections of human pancreas adenocarcinoma tissue. The recommended concentration is 5.0 µg/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results were achieved without antigen retrieval. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. Mouse IgG1 Azide Free Hu E, P, WB
0.5 mg / €190.00
  Acris Antibodies GmbH

Interleukin-10 / IL10 antibody

  Anti-IL10 Rat Monoclonal Antibody  
Anti-IL-10 antibody IHC of mouse small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. Rat IgG1 JES5-2A5 Purified Ms C, E, F
0.1 mg / €400.00
  OriGene Technologies, Inc.

Interleukin-10 / IL10 antibody

  Rabbit polyclonal anti-IL-10 antibody  
Anti IL-10 whole antiserum (TA319291) was tested against recombinant IL10. Recombinant IL-10 was run on a 4-20% reducing gel, and transferred to nitrocellulose. Membrane was blocked in 3% BSA-TBS for 1 Hour 4oC and probed with p/n TA319291 diluted 1:200 in 3% BSA-TBS o/n 4oC. Primary antibody was detected with Gt anti Rb 611-103-122 Lot#21231 1:20,000 in MB-070 1 Hour 4oC using FemtoMax Super sensitive chemiluminescent substrate. Rabbit Serum Hu C, E, FN, IP, R, WB
1 ml / €1,070.00
  OriGene Technologies, Inc.

Interleukin-10 / IL10 antibody

  Biotinylated Anti-Human IL-10 Rabbit Polyclonal Antibody  
To detect hIL-10 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-10. Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Interleukin-10 / IL10 antibody

  Anti-Human IL-10 Rabbit Polyclonal Antibody  
To detect hIL-10 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-10 (TA328224) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-10. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-10 / IL10 antibody

  Biotinylated Anti-Murine IL-10 Rabbit Polyclonal Antibody  
To detect mIL-10 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant mIL-10. Rabbit IgG Purified E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Interleukin-10 / IL10 antibody

  Anti-Murine IL-10 Rabbit Polyclonal Antibody  
To detect mIL-10 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine IL-10 (TA328494) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-10. Rabbit IgG Purified E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-10 / IL10 antibody

  Mouse Monoclonal Anti-IL-10 Antibody  
Interleukin-10 / IL10 Mouse IgG1 B-S10 Purified Hu C, E, Elispot, F
0.5 mg / €413.00
  OriGene Technologies, Inc.

Interleukin-10 / IL10 antibody

  Rabbit Polyclonal Anti-IL-10 Antibody  
Interleukin-10 / IL10 Rabbit IgG Purified Hu E, Elispot, WB
0.5 mg / €303.00
  OriGene Technologies, Inc.

Interleukin-10 / IL10 antibody

  Rat Monoclonal Anti-IL-10 Antibody  
Interleukin-10 / IL10 Rat IgM SXC-1 Purified Ms E, Elispot
0.5 mg / €413.00
  OriGene Technologies, Inc.

Interleukin-10 / IL10 antibody

  ELISA and Luminex validated lL-10 Rat monoclonal antibody clone (9D7, clone OTI9D7 (formerly 9D7)  
lL-10 ELISA with 9D7 Capture (TA600017) and 12G8 Detection (TA700017) Antibodies. Substrate used: Recombinant Human lL-10 (E. Coli expressed Peptide) Rat IgG1 OTI9D7 Purified Hu E, LMNX
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-10 / IL10 antibody

  ELISA and Luminex validated biotinylated lL-10 Rat monoclonal antibody clone (12G8), clone OTI12G8 (formerly 12G8)  
lL-10 ELISA with 9D7 Capture (TA600017) and 12G8 Detection (TA700017) Antibodies. Substrate used: Recombinant Human lL-10 (E. Coli expressed Peptide) Rat IgG1 OTI12G8 Purified Hu E, LMNX
50 µg / €345.00
  OriGene Technologies, Inc.

Interleukin-10 / IL10 antibody

Interleukin-10 / IL10 Rat IgG1 JES3-9D7 Azide Free Hu E, F, FN
1 mg / €800.00
  Acris Antibodies GmbH

Interleukin-10 / IL10 antibody

Interleukin-10 / IL10 Rat IgG1 JES3-9D7 Azide Free Hu E, F, FN
0.5 mg / €570.00
  Acris Antibodies GmbH

Interleukin-10 / IL10 antibody

Interleukin-10 / IL10 Mouse IgG1 945A5A10 Biotin Hu E(detection)
0.1 mg / €560.00
  Acris Antibodies GmbH

Interleukin-10 / IL10 antibody

Interleukin-10 / IL10 Rat IgG1 JES5-2A5 Azide Free Ms E, FN
0.5 mg / €700.00
  Acris Antibodies GmbH

Interleukin-10 / IL10 antibody

Interleukin-10 / IL10 Rat IgG1 JES5-2A5 Purified Ms E, FN
0.1 mg / €520.00
  Acris Antibodies GmbH

Interleukin-10 / IL10 antibody

Interleukin-10 / IL10 Mouse IgG1 945A5D11 Purified Hu E
0.5 mg / €560.00
  Acris Antibodies GmbH

Interleukin-10 / IL10 antibody

Interleukin-10 / IL10 Mouse IgG2b CC318 Purified Bov, Eq, Sh E, E(capture), Elispot, F
0.5 mg / €670.00
  Acris Antibodies GmbH

Items 1 to 20 of 164 total

per page
Page:
  1. 1
  2. 2
  3. 3
  4. 4
  5. 5
  • LinkedIn