Interleukin-10 / IL10 antibody

Principal name

Interleukin-10 / IL10 antibody

Alternative names for Interleukin-10 / IL10 antibody

IL-10, CSIF, TGIF, Cytokine synthesis inhibitory factor

SwissProt ID

P18893 (Mouse), P22301 (Human), P29456 (Rat), P43480 (Bovin), P48411 (Canfa), P55029 (Felca), Q28374 (Horse), Q29055 (Pig), Q29408 (Sheep), Q6A2H4 (Chick), Q9TSJ4 (Rabit), Q9Z1Y5 (Cavpo)

Gene ID

16153 (Il10), 25325, 3586 (IL10), 281246

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Eq (Equine), Gt (Goat), Sh (Sheep)

Available hosts

Mouse, Rabbit, Rat, Sheep

Available applications

Paraffin Sections (P), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Functional assay (FN), Flow Cytometry (F), ELISA (detection) (E(detection)), ELISA (capture) (E(capture)), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Luminex (LMNX), Radioimmunoassay (R)

Background of Interleukin-10 / IL10 antibody

Interleukin 10 (IL-10), initially designated cytokine synthesis inhibitory factor (CSIF), was originally identified as a product of murine T helper 2 (TH2) clones that suppressed the production of cytokines by T helper 1 (TH1) clones responding to antigen stimulation in the presence of monocyte/macrophage antigen-presenting cells .

The human homologue of mouse IL-10 was subsequently cloned by cross-hybridization . Human and mouse IL-10 are 81% and 73% identical at the nucleotide and amino acid levels, respectively. Mouse, rat and human IL-10 cDNAs encode 178 amino acid (aa) residue precursor polypeptides with hydrophobic signal peptides that are cleaved to generate 160 aa residue mature proteins. There is at least 73% aa identity between the proteins from the three species. In contrast to mouse IL-10, human IL-10 appears to be non-glycosylated. Both mouse and human IL-10 exist as non-covalently-linked homodimers in solution. Although human IL-10 is active on mouse cells, mouse IL-10 does not show species cross-reactivity on human cells.

IL-10 is a pleiotropic cytokine that can exert either immunosuppressive or immunostimulatory effects on a variety of cell types. human IL-10 can inhibit monocyte/macrophage-dependent, antigen-stimulated cytokine synthesis by human PBMNC and NK cells . L-10 can inhibit production or function of a macrophage membrane-bound costimulator required for activation of T cells and NK cells.

IL-10 is a potent modulator of monocyte/macrophage function. IL-10 also enhances the release of soluble TNF receptors and inhibits the expression of surface ICAM-1 and B7 . Finally, IL-10 has been reported to suppress the synthesis of superoxide anion plus reactive oxygen intermediates (ROI), and either inhibit or facilitate nitric oxide synthesis, depending on the time of exposure to activated macrophages . IL-10 has marked effects on B cells. IL-10 also has documented activity on endothelial cells, where it mimics IL-4 , and on thymocytes and mast cells, where it acts as a growth costimulator .

General readings

1. Chakravarti, A. Normal human primary CD4+ T lymphocytes synthesize and release functional osteoprotegerin in vitro. Laboratory Investigation.;88(2):171-84. 2/1/2008 18040268
Ward FJ, Clonal regulatory T cells specific for a red blood cell autoantigen in human autoimmune hemolytic anemia Blood, 2007, 111(2):680-7.
2. Darce, J.R. Regulated Expression of BAFF-Binding Receptors during Human B Cell Differentiation. The Journal of Immunology, 2007, 179(11):7276-86.
3. Tran DQ Induction of FOXP3 expression in naive human CD4+FOXP3 T cells by T-cell receptor stimulation is transforming growth factor-beta dependent but does not confer a regulatory phenotype Blood, 2007, 110(8):2983-90.
4. Sakuishi, K. Invariant NKT cells biased for IL-5 production act as crucial regulators of inflammation. The Journal of Immunology, 2007, 179(6):3452-62.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Interleukin-10 / IL10 antibody

This antibody stained formalin-fixed paraffin-embedded sections of human pancreas adenocarcinoma tissue. The recommended concentration is 5.0 µg/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results were achieved without antigen retrieval. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. Mouse IgG1 Azide Free Hu E, P, WB
0.5 mg / €220.00
  OriGene Technologies GmbH

Interleukin-10 / IL10 antibody

Interleukin-10 / IL10 Mouse IgG1 B-S10 Azide Free Hu E(capture), FN
0.5 mg / €620.00
  OriGene Technologies GmbH

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