Interleukin-10 / IL10 antibody
Principal name
Interleukin-10 / IL10 antibody
Alternative names for Interleukin-10 / IL10 antibody
IL-10, CSIF, TGIF, Cytokine synthesis inhibitory factor
SwissProt ID
P18893 (Mouse), P22301 (Human), P29456 (Rat), P43480 (Bovin), P48411 (Canfa), P55029 (Felca), Q28374 (Horse), Q29055 (Pig), Q29408 (Sheep), Q6A2H4 (Chick), Q9TSJ4 (Rabit), Q9Z1Y5 (Cavpo)
Ncbi ID
NP_034678.1, NP_036986.2, 9606, NP_034678, NP_000563, NP_036986, NP_776513.1, NP_776513, 10090, 10116, NP_000563.1, AAI04253.1, NM_000572, NM_010548, NM_012854
Available reactivities
Available hosts
Available applications
Paraffin Sections (P), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Functional assay (FN), Flow Cytometry (F), ELISA (detection) (E(detection)), ELISA (capture) (E(capture)), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Luminex (LMNX), Radioimmunoassay (R)
Background of Interleukin-10 / IL10 antibody
Interleukin 10 (IL-10), initially designated cytokine synthesis inhibitory factor (CSIF), was originally identified as a product of murine T helper 2 (TH2) clones that suppressed the production of cytokines by T helper 1 (TH1) clones responding to antigen stimulation in the presence of monocyte/macrophage antigen-presenting cells .
The human homologue of mouse IL-10 was subsequently cloned by cross-hybridization . Human and mouse IL-10 are 81% and 73% identical at the nucleotide and amino acid levels, respectively. Mouse, rat and human IL-10 cDNAs encode 178 amino acid (aa) residue precursor polypeptides with hydrophobic signal peptides that are cleaved to generate 160 aa residue mature proteins. There is at least 73% aa identity between the proteins from the three species. In contrast to mouse IL-10, human IL-10 appears to be non-glycosylated. Both mouse and human IL-10 exist as non-covalently-linked homodimers in solution. Although human IL-10 is active on mouse cells, mouse IL-10 does not show species cross-reactivity on human cells.
IL-10 is a pleiotropic cytokine that can exert either immunosuppressive or immunostimulatory effects on a variety of cell types. human IL-10 can inhibit monocyte/macrophage-dependent, antigen-stimulated cytokine synthesis by human PBMNC and NK cells . L-10 can inhibit production or function of a macrophage membrane-bound costimulator required for activation of T cells and NK cells.
IL-10 is a potent modulator of monocyte/macrophage function. IL-10 also enhances the release of soluble TNF receptors and inhibits the expression of surface ICAM-1 and B7 . Finally, IL-10 has been reported to suppress the synthesis of superoxide anion plus reactive oxygen intermediates (ROI), and either inhibit or facilitate nitric oxide synthesis, depending on the time of exposure to activated macrophages . IL-10 has marked effects on B cells. IL-10 also has documented activity on endothelial cells, where it mimics IL-4 , and on thymocytes and mast cells, where it acts as a growth costimulator .
General readings
1. Chakravarti, A. Normal human primary CD4+ T lymphocytes synthesize and release functional osteoprotegerin in vitro. Laboratory Investigation.;88(2):171-84. 2/1/2008 18040268
Ward FJ, Clonal regulatory T cells specific for a red blood cell autoantigen in human autoimmune hemolytic anemia Blood, 2007, 111(2):680-7.
2. Darce, J.R. Regulated Expression of BAFF-Binding Receptors during Human B Cell Differentiation. The Journal of Immunology, 2007, 179(11):7276-86.
3. Tran DQ Induction of FOXP3 expression in naive human CD4+FOXP3 T cells by T-cell receptor stimulation is transforming growth factor-beta dependent but does not confer a regulatory phenotype Blood, 2007, 110(8):2983-90.
4. Sakuishi, K. Invariant NKT cells biased for IL-5 production act as crucial regulators of inflammation. The Journal of Immunology, 2007, 179(6):3452-62.










Lane 1: Un-transfected 293T cells.
Lane 2: 239T cells transfected with human IL-6 gene.
Lane 3: 293T cells transfected with human IL-10 gene.


Lane 1&2: Recombinant human protein loaded at 10ng/well.

















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Primary Antibodies
Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
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TA318463 | Interleukin-10 / IL10 antibody |
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Anti-IL10 Rat Monoclonal Antibody | |||||||
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Rat | IgG1 | JES5-2A5 | Purified | Ms | C, E, F |
0.1 mg /
€425.00
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OriGene Technologies, Inc. | |||||||
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TA319291 | Interleukin-10 / IL10 antibody |
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Rabbit polyclonal anti-IL-10 antibody | |||||||
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Rabbit | Serum | Hu | C, E, FN, IP, R, WB |
1 ml /
€1,345.00
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OriGene Technologies, Inc. | |||||||
TA352739 | Interleukin-10 / IL10 antibody |
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Mouse Monoclonal Anti-IL-10 Antibody | |||||||
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Mouse | IgG1 | B-S10 | Purified | Hu | C, E, Elispot, F |
0.5 mg /
€415.00
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OriGene Technologies, Inc. | |||||||
AM39036FC-N | Interleukin-10 / IL10 antibody |
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Mouse | IgG1 | BN-10 | FITC | Hu | C, F |
100 Tests /
€530.00
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OriGene Technologies GmbH | |||||||
AM39036RP-N | Interleukin-10 / IL10 antibody |
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Mouse | IgG1 | BN-10 | PE | Hu | C, F |
100 Tests /
€630.00
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OriGene Technologies GmbH | |||||||
AP26386PU-N | Interleukin-10 / IL10 antibody |
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Rabbit | IgG | Purified | Hu | C, E, FN, WB |
0.1 mg /
€320.00
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OriGene Technologies GmbH | |||||||
DDX0320P-50 | Interleukin-10 / IL10 antibody |
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Rat | IgG2a | 12G8 | Purified | Hu | C, E, F, WB |
50 µg /
€340.00
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Dendritics SAS | |||||||
DDX0320P-100 | Interleukin-10 / IL10 antibody |
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Rat | IgG2a | 12G8 | Purified | Hu | C, E, F, WB |
0.1 mg /
€440.00
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Dendritics SAS |