Interleukin-12 alpha / IL12A antibody

Principal name

Interleukin-12 alpha / IL12A antibody

Alternative names for Interleukin-12 alpha / IL12A antibody

IL-12A, IL-12 subunit p35, NKSF1, CLMF p35, Cytotoxic lymphocyte maturation factor 35 kDa subunit, NK cell stimulatory factor chain 1

SwissProt ID

O02743 (Felca), P29459 (Human), P43431 (Mouse), P54349 (Bovin), Q28267 (Canfa), Q29053 (Pig), Q9R103 (Rat), Q9TU27 (Sheep), Q9XSQ6 (Horse)

Gene ID

3592 (IL12A), 16159 (Il12a), 281856, 84405

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Por (Porcine), Mky (Monkey), Bov (Bovine), Sh (Sheep)

Available hosts

Rabbit, Mouse, Goat, Rat, Sheep

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Functional assay (FN), Flow Cytometry (F), ELISA (capture) (E(capture)), ELISA (detection) (E(detection)), Paraffin Sections (P), Neutralisation (NEUT), Immunoprecipitation (IP), Frozen Sections (C)

Background of Interleukin-12 alpha / IL12A antibody

Interleukin-12 (IL-12) is a heterodimeric 70 kD (p70) cytokine composed of two covalently linked, glycosylated chains, 40kD (p40) and 35-kD (p35). IL-12 is mainly produced by monocytes, macrophages, and dendritic cells in response to bacterial products such as lipopolysaccharides (LPS), to intracellular pathogens or upon interaction with activated T cells. IL-12 was originally discovered because of its ability to induce interferon-gamma (IFN-g) production, cell proliferation, and cytotoxicity mediated by natural killer cells and T cells. It is now established that IL-12 also plays a key role in the development of Th1 responses, leading to IFN-g and IL-2 production. These cytokines can in turn promote T-cell responses and macrophage activation. sciences recombinant mouse IL-12 p70 is produced in baculovirus-infected insect cells as an authentic heterodimer of precursor p35 and p40 subunits using a dual promoter expression system. It is distinct from other available forms of the protein in that it is expressed as a true heterodimer, as opposed to a single-chain, pseudo-heterodimer in which the subunits are joined by an artificial linker.

General readings

Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Interleukin-12 (p35+p70) antibody

Interleukin-12 (p35+p70) Mouse IgG1 B-T21 Azide Free Hu F, FN
0.5 mg / €460.00
  Acris Antibodies GmbH

Interleukin-12 (p35+p70) antibody

Interleukin-12 (p35+p70) Mouse IgG1 B-T21 FITC Hu F
0.1 mg / €270.00
  Acris Antibodies GmbH

Interleukin-12 (p70+p40) antibody

Interleukin-12 (p70+p40) Mouse IgG1 B-P40 Azide Free Hu E(capture)
1 mg / €790.00
  Acris Antibodies GmbH

Interleukin-12 (p70+p40) antibody

Interleukin-12 (p70+p40) Mouse IgG1 B-P24 Biotin Hu E(detection), Elispot
0.1 mg / €460.00
  Acris Antibodies GmbH

Interleukin-12 alpha / IL12A antibody

Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-IL12A rabbit polyclonal antibody. (TA590054) Rabbit IgG Purified Hu E, P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Interleukin-12 alpha / IL12A antibody

To detect hIL-12 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-12. Goat IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Interleukin-12 alpha / IL12A antibody

To detect hIL-12 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-12 (TA328228) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-12. Goat IgG Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-12 alpha / IL12A antibody

To detect mIL-12 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant mIL-12. Goat IgG Purified E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Interleukin-12 alpha / IL12A antibody

To detect mIL-12 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine IL-12 (TA328496) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-12. Goat IgG Purified E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-12 alpha / IL12A antibody

Western blot analysis of Interleukin 12A expression in HeLa cells Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-12 alpha / IL12A antibody

Western blot analysis of IL-12A in HeLa cell lysate with IL-12A antibody at 0.5 ug/mL in (A) the absence and (B) the presence of blocking peptide. Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-12 alpha / IL12A antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-IL12A mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA808081)(1:150) Mouse IgG1 OTI2A8 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Interleukin-12 alpha / IL12A antibody

Immunohistochemistry using Interleukin-12 alpha / IL12A Antibody Cat.-No AM01362PU-N Mouse IgG1 Azide Free Hu E, ICC/IF, WB
0.5 mg / €220.00
  Acris Antibodies GmbH

Interleukin-12 alpha / IL12A antibody

Interleukin-12 alpha / IL12A Rat IgG2a C17.8 Azide Free Ms E, E(detection), F, ICC/IF, IP, NEUT, WB
0.1 mg / €200.00
  Acris Antibodies GmbH

Interleukin-12 alpha / IL12A (C-term) antibody

Western blot analysis of IL12A Antibody (C-term) (AP18069PU-N) in MDA-MB231 cell line lysates (35ug/lane). IL12A (arrow) was detected using the purified Pab. Rabbit Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Interleukin-12 alpha / IL12A antibody

Immunohistochemistry (IHC) analyzes of IL-12A antibody (Cat.-No.: AP20613PU-N) in paraffin-embedded human brain tissue. Rabbit Aff - Purified Hu, Ms, Rt P
0.1 mg / €350.00
  Acris Antibodies GmbH

Interleukin-12 alpha / IL12A antibody

Human Intestine, Paneth Cells: Formalin-Fixed, Paraffin-Embedded (FFPE). Goat Aff - Purified Bear, Bov, Can, Elephant, Eq, Fe, Ft, GP, Gt, Hu, Mky, Op, Por, Prim, Rb, Sh E, P
50 µg / €470.00
  Acris Antibodies GmbH

Interleukin-12 alpha / IL12A (alpha + beta) antibody

Interleukin-12 alpha / IL12A Rat IgG1 Azide Free Ms E, FN, WB
0.5 mg / €220.00
  Acris Antibodies GmbH

Interleukin-12 alpha / IL12A (alpha + beta) antibody

Interleukin-12 alpha / IL12A Goat Biotin Hu E, WB
25 µg / €230.00
  Acris Antibodies GmbH

Interleukin-12 alpha / IL12A (alpha + beta) antibody

Interleukin-12 alpha / IL12A Goat Biotin Hu E, WB
50 µg / €340.00
  Acris Antibodies GmbH

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