Interleukin-12 beta / IL12B antibody

Principal name

Interleukin-12 beta / IL12B antibody

Alternative names for Interleukin-12 beta / IL12B antibody

Interleukin-12B, IL-12B, IL-12 subunit p40, CLMF p40, NKSF2, Cytotoxic lymphocyte maturation factor 40 kDa subunit, NK cell stimulatory factor chain 2

SwissProt ID

O02744 (Felca), P29460 (Human), P43432 (Mouse), P46282 (Bovin), P68220 (Sheep), Q28268 (Canfa), Q28938 (Pig), Q924V5 (Cavpo), Q9XSQ5 (Horse)

Gene ID

3593 (IL12B), 3592 (IL12A), 281857, 16160 (Il12b)

Available reactivities

Hu (Human), Bov (Bovine), Sh (Sheep), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Goat, Sheep, Rat, Rabbit

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F), Functional assay (FN), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)

Background of Interleukin-12 beta / IL12B antibody

Interleukin-12 (IL12; formerly NKSF, for natural killer cell stimulatory factor, or CLMF, for cytotoxic lymphocyte maturation factor) is a novel cytokine cloned from B-cell lines. IL-12 is a heterodimeric molecule composed of p35 and p40 subunits.1 The larger 40-kDa subunit (p40) is a member of the cytokine receptor family, and the smaller 35-kDa subunit (p35) is related to IL6 and GCSF.2 Both IL-12 p40(-/-) and p35(-/-) mice fail to produce IL-12 p70 heterodimer.3 Interleukin (IL)-12 has been cloned on the basis of its ability to activate natural killer (NK) cells and promote the development of cytolytic T cells. With further understanding of its activities, IL-12 has emerged as an important cytokine, affecting both immune and hematologic functions. It has been shown to be necessary for the T cell independent induction of interferon (IFN)-gamma, critical for the initial suppression of bacterial and parasitic infection; for the development of a Th1 response, critical for effective host defense against intracellular pathogens; and for the activation of differentiated T lymphocytes of both CD4+ and CD8+ phenotype.4 The subunits map to different chromosomes: p40 (IL12B) to 5q31-q33 and p35 (IL12A) to 3p12-3q13.2.2 The standard product used in this kit is recombinant human IL-12(p40), which is a 40KDa glycoprotein consisting of 306 amino acids.

General readings

Romero, R., et al. Am. J. Obstet. Gynecol. 203 (4), 361 (2010)
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253 (2010)
Miteva, L.D., et al. Rheumatol. Int. (2010)
de Heens, G.L., et al. J. Clin. Periodontol. 37(2):129-136 (2010)
Sobti, R.C., et al. Folia Biol. (Praha) 56(3):110-115 (2010)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Interleukin-12 (p40+p70) antibody

Interleukin-12 (p40+p70) Mouse IgG1 B-P24 Azide Free Hu F, FN
0.5 mg / €460.00
  Acris Antibodies GmbH

Interleukin-12 (p40+p70) antibody

Interleukin-12 (p40+p70) Mouse IgG1 B-P24 FITC Hu F
0.1 mg / €270.00
  Acris Antibodies GmbH

Interleukin-12 (p70+p40) antibody

Interleukin-12 (p70+p40) Mouse IgG1 B-P40 Azide Free Hu F
0.5 mg / €460.00
  Acris Antibodies GmbH

Interleukin-12 beta / IL12B antibody

(1ug/ml) staining of Rat Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-12 beta / IL12B antibody

TA302515 (0.1µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-12 beta / IL12B antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL12B (RC209679, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL12B.(1:2000) Mouse IgG1 OTI1A3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Interleukin-12 beta / IL12B antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-IL12B mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807498) Mouse IgG1 OTI8B9 Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Interleukin-12 beta / IL12B antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL12B (RC209679, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL12B.(1:2000) Mouse IgG1 OTI3H6 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Interleukin-12 beta / IL12B antibody

Interleukin-12 beta / IL12B Mouse IgG2a CC301 Low Endotoxin Bov, Hu, Sh E, Elispot, F, FN, WB
0.5 mg / €680.00
  Acris Antibodies GmbH

Interleukin-12 beta / IL12B antibody

Interleukin-12 beta / IL12B Mouse IgG2b CC326 Azide Free Bov, Hu, Sh E, F, FN
0.5 mg / €670.00
  Acris Antibodies GmbH

Interleukin-12 beta / IL12B antibody

Western blot (WB) analysis of IL-12B antibody (Cat.-No.: AP20604PU-N) in extracts from K562 cells. Rabbit Aff - Purified Hu WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Interleukin-12 beta / IL12B (C-term) antibody

Immunohistochemistry analysis in human tonsil tissue (formalin-fixed, paraffin-embedded) using Interleukin-12 beta/IL12Bantibody Cat.-No. AP52183PU-N (C-term), followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the IL12B antibody (C-term) for IHC. Clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu F, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Interleukin-12 beta / IL12B antibody

Interleukin-12 beta / IL12B Mouse IgG1 1-1A4 Purified Hu C, E
0.5 mg / €440.00
  Acris Antibodies GmbH

Interleukin-12 beta / IL12B antibody

IL12B polyclonal antibody (A01), Lot # 050912JC01. Western Blot analysis of IL12B expression in human ovary cancer. Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

Interleukin-12 beta / IL12B antibody

Interleukin-12 beta / IL12B Mouse IgG2a 2H6 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Interleukin-12 beta / IL12B antibody

The lysates of Jurkat ( lane 1 ) and rat serum ( lane 2 ) ( 40 µg ) were resolved by SDS-PAGE and probed with IL12B monoclonal antibody, clone AT1D6 ( Cat # MAB2067 ) ( 1:1000 ). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG1 AT1D6 Hu, Rt E, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

Interleukin-12 beta / IL12B antibody

Interleukin-12 beta / IL12B Rat IgG2b Ms E, WB
0.5 mg / €400.00
  Abnova Taiwan Corp.

Interleukin-12 beta / IL12B antibody

IL12B polyclonal antibody ( Cat # PAB7305 ) ( 0.1 µg/ml ) staining of human liver lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

Interleukin-12 beta / IL12B antibody

The Il12b polyclonal antibody ( Cat # PAB7306 ) ( 1 µg/ml ) staining of rat liver lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Ms, Rt E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

Interleukin-12 beta / IL12B antibody

Interleukin-12 beta / IL12B Sheep Hu E
1 ml / €560.00
  Abnova Taiwan Corp.

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