Interleukin-12 beta / IL12B antibody

Principal name

Interleukin-12 beta / IL12B antibody

Alternative names for Interleukin-12 beta / IL12B antibody

Interleukin-12B, IL-12B, IL-12 subunit p40, CLMF p40, NKSF2, Cytotoxic lymphocyte maturation factor 40 kDa subunit, NK cell stimulatory factor chain 2

SwissProt ID

O02744 (Felca), P29460 (Human), P43432 (Mouse), P46282 (Bovin), P68220 (Sheep), Q28268 (Canfa), Q28938 (Pig), Q924V5 (Cavpo), Q9XSQ5 (Horse)

Gene ID

281857 (IL12B), 3593, 16160 (Il12b)

Available reactivities

Bov (Bovine), Hu (Human), Sh (Sheep), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit, Rat, Goat, Sheep

Available applications

Enzyme Immunoassay (E), Functional assay (FN), Flow Cytometry (F), Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C)

Background of Interleukin-12 beta / IL12B antibody

Interleukin 12 (IL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine originally identified in the medium of a human B-lymphoblastoid cell line .

Biologically active mouse IL-12 is a disulfide-linked, 70 kDa (p70) heterodimeric glycoprotein composed of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. While the p40 and p35 subunits by themselves do not have IL-12 activity, the p40 homodimer has been shown to bind the IL-12 receptor and is an IL-12 antagonist . Mature mouse p35 subunit is composed of 193 amino acid (aa) residues and contains seven cysteines plus one potential N-linked glycosylation site . Mature mouse p40 subunit has 313 aa, with 13 cysteines and five potential N-linked glycosylation sites . Mouse p35 and p40 subunits show 63% and 72% aa identity, respectively, to the human p35 and p40 subunits . Although mouse IL-12 is active on both human and mouse cells, human IL-12 is only active on human cells.

IL-12 has been shown to have multiple effects on T lymphocytes and natural killer (NK) cells. Some of these effects include the induction of IFN-. and TNF production by T and NK cells, the enhancement of cytotoxic activity of T and NK cells and the stimulation of T and NK cell proliferation. IL-12 has also been shown to be a central mediator of the cell-mediated immune response by promoting Th1 development .

Cell surface staining for IL-12 on a human monocytic and a mouse macrophage cell line has been reported, suggesting that membrane-associated IL-12 may exist . Cells known to produce IL-12 include macrophages, dendritic cells, monocytes, Langerhans cells, neutrophils, and keratinocytes. Although a human B cell line has been shown to produce IL-12, fresh B cells are apparently not producers of IL-12.

General readings

Kobayashi, M. et al. (1989) J. Exp. Med. 170:827.

Stern, A.S. et al. (1990) Proc. Natl. Acad. Sci. USA 87:6808.

Gubler, U. et al. (1991) Proc. Natl. Acad. Sci. USA 88:4143.

Gillessen, S. et al. (1995) Eur. J. Immunol. 25:200.

Ling, P. et al. (1995) J. Immunol. 154:116

Schoenhaut, D.S. et al. (1992) J. Immunol. 148:3433.

Trinchieri, G. (1995) Annu. Rev. Immunol. 13:251.

Pudda, P. et al. (1997) J. Immunol. 159:3490.

Windhagen, A. et al. (1996) J. Immunol. 157:1127.

Mehrotra, P.T. et al. (1998) J. Immunol. 160:2637.

Wolf, S. F. et al. (1994) Stem Cells 12:154.

Fan, X. et al. (1996) Biochem. Biophys. Res. Commun. 225:1063.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Interleukin-12 (p40+p70) antibody

Interleukin-12 (p40+p70) Mouse IgG1 B-P24 Azide Free Hu F, FN
0.5 mg / €460.00
  Acris Antibodies GmbH

Interleukin-12 (p40+p70) antibody

Interleukin-12 (p40+p70) Mouse IgG1 B-P24 FITC Hu F
0.1 mg / €270.00
  Acris Antibodies GmbH

Interleukin-12 (p70+p40) antibody

Interleukin-12 (p70+p40) Mouse IgG1 B-P40 Azide Free Hu F
0.5 mg / €460.00
  Acris Antibodies GmbH

Interleukin-12 beta / IL12B antibody

  Goat Anti-Il12b / Il12p40 Antibody  
(1ug/ml) staining of Rat Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-12 beta / IL12B antibody

  Goat Polyclonal Antibody against IL12B / IL12p40  
TA302515 (0.1µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-12 beta / IL12B antibody

  Mouse Monoclonal Anti-IL-12/23p40 Antibody  
Interleukin-12 beta / IL12B Mouse IgG1 B-P24 Purified Hu C, E, Elispot
0.5 mg / €413.00
  OriGene Technologies, Inc.

Interleukin-12 beta / IL12B antibody

  Mouse Monoclonal Anti-IL-12/23p40 Antibody  
Interleukin-12 beta / IL12B Mouse IgG1 AW-1 Purified Hu
0.5 mg / €413.00
  OriGene Technologies, Inc.

Interleukin-12 beta / IL12B antibody

  IL12B mouse monoclonal antibody, clone OTI1A3 (formerly 1A3)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL12B (RC209679, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL12B.(1:2000) Mouse IgG1 OTI1A3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Interleukin-12 beta / IL12B antibody

  IL12B mouse monoclonal antibody, clone OTI8B9 (formerly 8B9)  
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-IL12B mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807498) Mouse IgG1 OTI8B9 Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Interleukin-12 beta / IL12B antibody

  IL12B mouse monoclonal antibody, clone OTI3H6 (formerly 3H6)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL12B (RC209679, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL12B.(1:2000) Mouse IgG1 OTI3H6 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Interleukin-12 beta / IL12B antibody

Interleukin-12 beta / IL12B Mouse IgG2a CC301 Low Endotoxin Bov, Hu, Sh E, Elispot, F, FN, WB
0.5 mg / €680.00
  Acris Antibodies GmbH

Interleukin-12 beta / IL12B antibody

Interleukin-12 beta / IL12B Mouse IgG2b CC326 Azide Free Bov, Hu, Sh E, F, FN
0.5 mg / €670.00
  Acris Antibodies GmbH

Interleukin-12 beta / IL12B antibody

Western blot (WB) analysis of IL-12B antibody (Cat.-No.: AP20604PU-N) in extracts from K562 cells. Rabbit Aff - Purified Hu WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Interleukin-12 beta / IL12B (C-term) antibody

Immunohistochemistry analysis in human tonsil tissue (formalin-fixed, paraffin-embedded) using Interleukin-12 beta/IL12Bantibody Cat.-No. AP52183PU-N (C-term), followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the IL12B antibody (C-term) for IHC. Clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu F, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Interleukin-12 beta / IL12B antibody

Interleukin-12 beta / IL12B Mouse IgG1 1-1A4 Purified Hu C, E
0.5 mg / €440.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

IL12B - human gene knockout kit via CRISPR

IL12B - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

IL12B - human gene knockout kit via CRISPR

IL12B - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

IL12B - human gene knockout kit via CRISPR

IL12B - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

IL12B - human gene knockout kit via CRISPR

IL12B - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Il12b - mouse gene knockout kit via CRISPR

Il12b - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

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