Interleukin-12 beta / IL12B antibody

Principal name

Interleukin-12 beta / IL12B antibody

Alternative names for Interleukin-12 beta / IL12B antibody

Interleukin-12B, IL-12B, IL-12 subunit p40, CLMF p40, NKSF2, Cytotoxic lymphocyte maturation factor 40 kDa subunit, NK cell stimulatory factor chain 2

SwissProt ID

O02744 (Felca), P29460 (Human), P43432 (Mouse), P46282 (Bovin), P68220 (Sheep), Q28268 (Canfa), Q28938 (Pig), Q924V5 (Cavpo), Q9XSQ5 (Horse)

Gene ID

281857 (IL12B), 3593, 16160 (Il12b)

Available reactivities

Bov (Bovine), Hu (Human), Sh (Sheep), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit, Rat, Goat, Sheep

Available applications

Enzyme Immunoassay (E), Functional assay (FN), Flow Cytometry (F), Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C)

Background of Interleukin-12 beta / IL12B antibody

Interleukin 12 (IL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine originally identified in the medium of a human B-lymphoblastoid cell line .

Biologically active mouse IL-12 is a disulfide-linked, 70 kDa (p70) heterodimeric glycoprotein composed of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. While the p40 and p35 subunits by themselves do not have IL-12 activity, the p40 homodimer has been shown to bind the IL-12 receptor and is an IL-12 antagonist . Mature mouse p35 subunit is composed of 193 amino acid (aa) residues and contains seven cysteines plus one potential N-linked glycosylation site . Mature mouse p40 subunit has 313 aa, with 13 cysteines and five potential N-linked glycosylation sites . Mouse p35 and p40 subunits show 63% and 72% aa identity, respectively, to the human p35 and p40 subunits . Although mouse IL-12 is active on both human and mouse cells, human IL-12 is only active on human cells.

IL-12 has been shown to have multiple effects on T lymphocytes and natural killer (NK) cells. Some of these effects include the induction of IFN-. and TNF production by T and NK cells, the enhancement of cytotoxic activity of T and NK cells and the stimulation of T and NK cell proliferation. IL-12 has also been shown to be a central mediator of the cell-mediated immune response by promoting Th1 development .

Cell surface staining for IL-12 on a human monocytic and a mouse macrophage cell line has been reported, suggesting that membrane-associated IL-12 may exist . Cells known to produce IL-12 include macrophages, dendritic cells, monocytes, Langerhans cells, neutrophils, and keratinocytes. Although a human B cell line has been shown to produce IL-12, fresh B cells are apparently not producers of IL-12.

General readings

Kobayashi, M. et al. (1989) J. Exp. Med. 170:827.

Stern, A.S. et al. (1990) Proc. Natl. Acad. Sci. USA 87:6808.

Gubler, U. et al. (1991) Proc. Natl. Acad. Sci. USA 88:4143.

Gillessen, S. et al. (1995) Eur. J. Immunol. 25:200.

Ling, P. et al. (1995) J. Immunol. 154:116

Schoenhaut, D.S. et al. (1992) J. Immunol. 148:3433.

Trinchieri, G. (1995) Annu. Rev. Immunol. 13:251.

Pudda, P. et al. (1997) J. Immunol. 159:3490.

Windhagen, A. et al. (1996) J. Immunol. 157:1127.

Mehrotra, P.T. et al. (1998) J. Immunol. 160:2637.

Wolf, S. F. et al. (1994) Stem Cells 12:154.

Fan, X. et al. (1996) Biochem. Biophys. Res. Commun. 225:1063.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Interleukin-12 beta / IL12B antibody

  Mouse Monoclonal Anti-IL-12/23p40 Antibody  
Interleukin-12 beta / IL12B Mouse IgG1 B-P24 Purified Hu C, E, Elispot
0.5 mg / €415.00
  OriGene Technologies, Inc.

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