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Interleukin-13 / IL13 antibody

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Principal name

Interleukin-13 / IL13 antibody

Alternative names for Interleukin-13 / IL13 antibody

IL-13, NC30

SwissProt ID

P20109 (Mouse), P35225 (Human), P42203 (Rat), Q95J68 (Pig), Q9N0W9 (Canfa), Q9XSV9 (Bovin)

Gene ID

3596 (IL13), 16163 (Il13), 116553, 396721, 1123596 (Q99VF6)

Ncbi ID

NP_002179.2, NP_032381.1, NP_446280.1, 10090, NP_998968.1, NP_446280, NP_002179, P35225, NM_002188, NP_032381

Available reactivities

Hu (Human), Ms (Mouse), Por (Porcine), Rt (Rat), Bov (Bovine), Sh (Sheep), Can (Canine), Gt (Goat)

Available hosts

Rabbit, Mouse, Rat, Sheep

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Functional assay (FN), Paraffin Sections (P), Neutralisation (NEUT), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (capture) (E(capture)), ELISA (detection) (E(detection))

Background of Interleukin-13 / IL13 antibody

Human IL-13 is a nonglycosylated protein of 132 amino acids with a molecular weight of 12 kDa. The IL-13 gene is located on chromosome 5q23-31, in the same region as the genes encoding IL-3, IL-4, IL-5 and GM-CSF. It is produced by activated CD4+ and CD8+ T cells, but the expression seems to be more abundant in CD4+ T cells. Although it is a Th2 cytokine in the mouse, it appears to be produced by Th0, Th1, and Th2-like human T cell clones. IL-13 induces CD23 expression on B cells, promotes B cell proliferation in combination with anti-Ig or CD40 antibodies, and stimulates secretion IgE, and IgG4. IL-13 has also been shown to prolong survival of human monocytes and increases surface expression of MHC class II, CD23, and members of the integrin superfamily, like CD11b, CD11c, CD18, CD29 and CD49e. IL-13 inhibits the production of a series of cytokines like IL-1, IL-6, TNF-alpha, and IL-8 by activated human monocytes. IL-13 induces IFN-gamma production by NK cells. The capacity of IL-13 to induce IgE synthesis indicates that IL-13 may play an important regulatory role in IgE-mediated atopic diseases. The measurement of IL-13 in body fluids may thus provide further information about the pathophysiology of atopic diseases. Furthermore, IL-13 inhibits HIV-1 replication in primary culture-derived macrophages and represents a candidate cytokine for the suppression of HIV infection within monocytes and macrophages in vivo.

General readings

General / background references:McKenzie AN et al. Structural comparison and chromosomal localization of the human and mouse IL-13 genes. J Immunol 150:5436-44 (1993). PubMed PMID: 8099936
McKenzie AN et al. Interleukin 13, a T-cell-derived cytokine that regulates human monocyte and B-cell function. Proc Natl Acad Sci U S A 90:3735-9 (1993). PubMed PMID: 8097324
Doherty TM et al. Modulation of murine macrophage function by IL-13. J Immunol 151:7151-60 (1993). PubMed PMID: 7903102
Cherwinski HM et al. Two types of mouse helper T cell clone. III. Further differences in lymphokine synthesis between Th1 and Th2 clones revealed by RNA hybridization, functionally monospecific bioassays, and monoclonal antibodies. J Exp Med 166:1229-44 (1987). PubMed PMID: 2960769

H00003596-M07 - Interleukin-13 / IL13
Immunoperoxidase of monoclonal antibody to IL13 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml]
AP09163PU-N - Interleukin-13 / IL13
Western blot using Protein-A Purified Anti-swine IL-13 antibody shows detection of recombinant swine IL-13 at 13.2kDa (arrow) raised in yeast. Multiple bands are expected of the glycosylated protein. Protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA (BSA-30, diluted 1:10), and probed with Rockland Immunochemicals, Inc. Anti-swine IL-13. After washing, membrane was probed with Dylight(TM) 649 Conjugated Anti-Rabbit IgG (H&L) (Donkey) Antibody
AM20534AF-N - Interleukin-13 / IL13
Formalin-Fixed, Paraffin-Embedded Rat Spleen stained with Interleukin-13 / IL13 Antibody Cat.-No AM20534AF-N at 10 µg/ml after heat-induced antigen retrieval.
NBP1-42776 - Interleukin-13 / IL13
Western Blot: IL13 Antibody [NBP1-42776] - Protein-A Purified Anti-swine IL-13 antibody shows detection of recombinant swine IL-13 at 13.2kDa (arrow) raised in yeast. Multiple bands are expected of the glycosylated protein.
NBP1-89119 - Interleukin-13 / IL13
Immunohistochemistry-Paraffin: IL13 Antibody [NBP1-89119] Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.
GTX37656 - Interleukin-13 / IL13
IHC-P analysis of human placenta tissue using IL-13 antibody.
GTX59763 - Interleukin-13 / IL13
Western blot analysis of IL-13 expression using recombinant mouse IL-13.
bs-0560R - Interleukin-13 / IL13
Mouse spleen cells probed with Rabbit Anti-IL-13 Polyclonal Antibody, FITC Conjugated (bs-0560R-FITC) (green) at 1:50 for 40 minutes compared to control cells (red).
bs-0560R - Interleukin-13 / IL13
Formalin-fixed and paraffin embedded human placenta tissue labeled with Anti-IL-13 Polyclonal Antibody, Unconjugated (bs-0560R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
CEK1209 - Human IL-13 ELISA Kit
Representative Standard Curve
CEK1493 - Mouse IL-13 ELISA Kit
Representative Standard Curve
CEK1494 - Mouse IL-13 ELISA Kit
Representative Standard Curve
CEK1612 - Rat IL-13 ELISA Kit
Representative Standard Curve
TA804004 - Interleukin-13 / IL13
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL13 (RC216947, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL13.
TP700054 - Interleukin-13 / IL13
TP723188 - Interleukin-13 / IL13
TP723189 - Interleukin-13 / IL13
TP723190 - Interleukin-13 / IL13
TP723191 - Interleukin-13 / IL13
TP723192 - Interleukin-13 / IL13
TP723715 - Interleukin-13 / IL13
TP723749 - Interleukin-13 / IL13

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Interleukin-13 / IL13 antibody
Western blot analysis of IL-13 expression using recombinant mouse IL-13. Rabbit Purified Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-13 / IL13 antibody
Interleukin-13 / IL13 Rat eBio13A Purified Ms E
50 µg / €120.00
  OriGene Technologies, Inc.

Interleukin-13 / IL13 antibody
To detect hIL-13 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-13. Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.
+1 additional image

Interleukin-13 / IL13 antibody
To detect hIL-13 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-13 (TA328230) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-13. Rabbit IgG Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.
+1 additional image

Interleukin-13 / IL13 antibody
To detect mIL-13 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant mIL-13. Rabbit IgG Purified E, WB
50 µg / €325.00
  OriGene Technologies, Inc.
+1 additional image

Interleukin-13 / IL13 antibody
To detect mIL-13 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine IL-13 (TA328498) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-13. Rabbit IgG Purified E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.
+1 additional image

Interleukin-13 / IL13 antibody
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL13 (RC216947, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL13. Mouse IgG1 OTI6D3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.
 

TrueMAB

Interleukin-13 / IL13 antibody
Interleukin-13 / IL13 Rat IgG2a JES10-35G12 Purified Hu E
0.5 mg / €450.00
  Acris Antibodies GmbH

Interleukin-13 / IL13 antibody
Interleukin-13 / IL13 Rat IgG1 JES10-2E10 Biotin Hu E
0.5 mg / €540.00
  Acris Antibodies GmbH

Interleukin-13 / IL13 antibody
Interleukin-13 / IL13 Mouse IgG1 B-P6 FITC Hu F
100 Tests / €360.00
  Acris Antibodies GmbH

Interleukin-13 / IL13 antibody
Formalin-Fixed, Paraffin-Embedded Rat Spleen stained with Interleukin-13 / IL13 Antibody Cat.-No AM20534AF-N at 10 µg/ml after heat-induced antigen retrieval. Mouse IgG1 A130D 12G5 1E4 Azide Free Rt E, F, P, WB
0.125 mg / €470.00
  Acris Antibodies GmbH

Interleukin-13 / IL13 antibody
Interleukin-13 / IL13 Mouse IgG1 B-B13 Azide Free Hu F, FN
0.5 mg / €460.00
  Acris Antibodies GmbH

Interleukin-13 / IL13 antibody
Interleukin-13 / IL13 Mouse IgG1 B-B13 FITC Hu F
0.1 mg / €270.00
  Acris Antibodies GmbH

Interleukin-13 / IL13 antibody
Interleukin-13 / IL13 Mouse IgG1 B-B13 Azide Free Hu E(capture), Elispot
1 mg / €790.00
  Acris Antibodies GmbH

Interleukin-13 / IL13 antibody
Interleukin-13 / IL13 Mouse IgG1 B-P6 Biotin Hu E(detection), Elispot
0.1 mg / €460.00
  Acris Antibodies GmbH

Interleukin-13 / IL13 antibody
Western blot using Protein-A Purified Anti-swine IL-13 antibody shows detection of recombinant swine IL-13 at 13.2kDa (arrow) raised in yeast. Multiple bands are expected of the glycosylated protein. Protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA (BSA-30, diluted 1:10), and probed with Rockland Immunochemicals, Inc. Anti-swine IL-13. After washing, membrane was probed with Dylight(TM) 649 Conjugated Anti-Rabbit IgG (H&L) (Donkey) Antibody Rabbit IgG Aff - Purified Por E, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

Interleukin-13 / IL13 antibody
Western blot using Protein-A Purified Anti-swine IL-13 antibody shows detection of recombinant swine IL-13 at 13.2kDa (arrow) raised in yeast. Multiple bands are expected of the glycosylated protein. Protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA (BSA-30, diluted 1:10), and probed with Rockland Immunochemicals, Inc. Anti-swine IL-13. After washing, membrane was probed with Dylight(TM) 649 Conjugated Anti-Rabbit IgG (H&L) (Donkey) Antibody Rabbit IgG Aff - Purified Por E, WB
25 µl / €190.00
  Acris Antibodies GmbH

Interleukin-13 / IL13 antibody
Interleukin-13 / IL13 Rabbit Biotin Hu E, WB
25 µg / €230.00
  Acris Antibodies GmbH

Interleukin-13 / IL13 antibody
Interleukin-13 / IL13 Rabbit Biotin Hu E, WB
50 µg / €340.00
  Acris Antibodies GmbH

Interleukin-13 / IL13 antibody
Interleukin-13 / IL13 Rabbit Aff - Purified Hu E, FN, WB
50 µg / €230.00
  Acris Antibodies GmbH

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