Interleukin-13 / IL13 antibody

Principal name

Interleukin-13 / IL13 antibody

Alternative names for Interleukin-13 / IL13 antibody

IL-13, NC30

SwissProt ID

P20109 (Mouse), P35225 (Human), P42203 (Rat), Q95J68 (Pig), Q9N0W9 (Canfa), Q9XSV9 (Bovin)

Gene ID

3596 (IL13), 16163 (Il13), 116553, 396721

Available reactivities

Hu (Human), Ms (Mouse), Por (Porcine), Rt (Rat)

Available hosts

Rabbit, Mouse, Rat, Sheep

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Functional assay (FN), Paraffin Sections (P), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (capture) (E(capture)), ELISA (detection) (E(detection))

Background of Interleukin-13 / IL13 antibody

Interleukin-13 (IL-13) is a 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergy, cancer, and tissue fibrosis . It is secreted by Th1, Th2, Th17, NK, and mast cells, visceral smooth muscle cells, eosinophils, and basophils . This pattern is similar to the expression of Interleukin-4 (IL-4) but also includes subsets of Th1 and Th17 cells that do not secrete IL-4 . IL-13 circulates as a monomer and has two internal disulfide bonds that contribute to its bundled four .-helix configuration . Mature human IL-13 shares approximately 58% amino acid sequence identity with mouse and rat IL-13. Despite the low homology, it exhibits cross-species activity between human, mouse, and rat . IL-13 suppresses the production of proinflammatory cytokines and other cytotoxic substances by macrophages, fibroblasts, and endothelial cells. On B cells, it promotes cellular activation, immunoglobulin class switching to IgE, and the upregulation of CD23/Fc. RII. Polymorphisms and upregulation of IL-13 are associated with atopy, asthma, airway hyperresponsiveness, and tissue fibrosis .

The biological effects of IL-13 and IL-4 are closely related due in part to a shared receptor system. IL-13 binds with low affinity to the transmembrane IL-13 R.1 which then forms a signaling complex with the transmembrane IL-4 R. . This high affinity receptor complex also functions as the type 2 IL-4 receptor . Soluble forms of IL-4 R. are expressed which retain ligand binding properties and inhibit IL-4 bioactivity . IL-4 R. also associates with the common gamma chain (.c) to form the type 1 IL-4 receptor complex .

Additionally, IL-13 binds with high affinity to IL-13 R.2 which is expressed as cell surface and soluble forms . IL-13 R.2 functions as a decoy receptor by preventing IL-13 from signaling through the IL-13 R.1/IL-4 R. complex . It also inhibits responsiveness to IL-4 by blocking signaling through IL-4-occupied IL-13 R.1/IL-4 R. receptor complexes . IL-13 R.2 is upregulated during Th2-biased immune responses and limits inflammatory tissue damage . Aside from its decoy function, IL-13 R.2 can signal in response to IL-13 to directly promote tumor cell invasiveness and the development of tissue fibrosis .

General readings

Townley, R.G. et al. (2011) Discov. Med. 12:513.

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Joshi, B.H. et al. (2006) Vitam. Horm. 74:479.

McKenzie, A.N. et al. (1993) Proc. Natl. Acad. Sci. USA 90:3735.

Gallo, E. et al. (2012) Eur. J. Immunol. 42:2322.

Moy, F.J. et al. (2001) J. Mol. Biol. 310:219.

Eisenmesser, E.Z. et al. (2001) J. Mol. Biol. 310:231.

Ruetten, H. and C. Thiemermann (1997) Shock 8:409.

Lakkis, F.G. et al. (1997) Biochem. Biophys. Res. Commun. 235:529.

Liu, Y. et al. (2012) Front. Immunol. 3:1.

Aman, M.J. et al. (1996) J. Biol. Chem. 271:29265.

Zurawski, S.M. et al. (1995) J. Biol. Chem. 270:13869.

Andrews, A.L. et al. (2002) J. Biol. Chem. 277:46073.

Kruse, S. et al. (1999) Int. Immunol. 11:1965.

Jung, T. et al. (1999) Int. Arch. Allergy Immunol. 119:23.

Kondo, M. et al. (1993) Science 262:1874.

Russell, S.M. et al. (1993) Science 262:1880.

Chen, W. et al. (2008) J. Allergy Clin. Immunol. 122:625.

Chen, W. et al. (2009) J. Immunol. 183:7870.

Daines, M.O. et al. (2007) J. Allergy Clin. Immunol. 119:375.

Kasaian, M.T. et al. (2011) J. Immunol. 187:561.

Andrews, A.-L. et al. (2006) J. Allergy Clin. Immunol. 118:858.

Rahaman, S.O. et al. (2002) Cancer Res. 62:1103.

Sivaprasad, U. et al. (2010) J. Immunol. 185:6802.

Chiaramonte, M.G. et al. (2003) J. Exp. Med. 197:687.

Fujisawa, T. et al. (2009) Cancer Res. 69:8678.

Fujisawa, T. et al. (2011) Int. J. Cancer 131:344.

Fichtner-Feigl, S. et al. (2006) Nat. Med. 12:99.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Interleukin-13 / IL13 antibody

  Rabbit polyclonal anti-IL-13 antibody  
Western blot analysis of IL-13 expression using recombinant mouse IL-13. Rabbit Purified Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-13 / IL13 antibody

  Rat Anti-Mouse IL-13 Purified (50 ug)  
Interleukin-13 / IL13 Rat eBio13A Purified Ms E
50 µg / €120.00
  OriGene Technologies, Inc.

Interleukin-13 / IL13 antibody

  Biotinylated Anti-Human IL-13 Rabbit Polyclonal Antibody  
To detect hIL-13 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-13. Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Interleukin-13 / IL13 antibody

  Anti-Human IL-13 Rabbit Polyclonal Antibody  
To detect hIL-13 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-13 (TA328230) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-13. Rabbit IgG Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-13 / IL13 antibody

  Biotinylated Anti-Murine IL-13 Rabbit Polyclonal Antibody  
To detect mIL-13 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant mIL-13. Rabbit IgG Purified E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Interleukin-13 / IL13 antibody

  Anti-Murine IL-13 Rabbit Polyclonal Antibody  
To detect mIL-13 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine IL-13 (TA328498) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-13. Rabbit IgG Purified E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-13 / IL13 antibody

  Mouse Monoclonal Anti-IL-13 Antibody  
Interleukin-13 / IL13 Mouse IgG1 B-B13 Purified Hu E, Elispot, F
0.5 mg / €413.00
  OriGene Technologies, Inc.

Interleukin-13 / IL13 antibody

  IL13 mouse monoclonal antibody, clone OTI6D3 (formerly 6D3)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL13 (RC216947, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL13. Mouse IgG1 OTI6D3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Interleukin-13 / IL13 antibody

Interleukin-13 / IL13 Rat IgG2a JES10-35G12 Purified Hu E
0.5 mg / €450.00
  Acris Antibodies GmbH

Interleukin-13 / IL13 antibody

Interleukin-13 / IL13 Rat IgG1 JES10-2E10 Biotin Hu E
0.5 mg / €540.00
  Acris Antibodies GmbH

Interleukin-13 / IL13 antibody

Interleukin-13 / IL13 Mouse IgG1 B-P6 FITC Hu F
100 Tests / €360.00
  Acris Antibodies GmbH

Interleukin-13 / IL13 antibody

Formalin-Fixed, Paraffin-Embedded Rat Spleen stained with Interleukin-13 / IL13 Antibody Cat.-No AM20534AF-N at 10 µg/ml after heat-induced antigen retrieval. Mouse IgG1 A130D 12G5 1E4 Azide Free Rt E, F, P
0.125 mg / €470.00
  Acris Antibodies GmbH

Interleukin-13 / IL13 antibody

Interleukin-13 / IL13 Mouse IgG1 B-B13 Azide Free Hu F, FN
0.5 mg / €460.00
  Acris Antibodies GmbH

Interleukin-13 / IL13 antibody

Interleukin-13 / IL13 Mouse IgG1 B-B13 FITC Hu F
0.1 mg / €270.00
  Acris Antibodies GmbH

Interleukin-13 / IL13 antibody

Interleukin-13 / IL13 Mouse IgG1 B-B13 Azide Free Hu E(capture), Elispot
1 mg / €790.00
  Acris Antibodies GmbH

Interleukin-13 / IL13 antibody

Interleukin-13 / IL13 Mouse IgG1 B-P6 Biotin Hu E(detection), Elispot
0.1 mg / €460.00
  Acris Antibodies GmbH

Interleukin-13 / IL13 antibody

Western blot using Protein-A Purified Anti-swine IL-13 antibody shows detection of recombinant swine IL-13 at 13.2kDa (arrow) raised in yeast. Multiple bands are expected of the glycosylated protein. Protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA (BSA-30, diluted 1:10), and probed with Rockland Immunochemicals, Inc. Anti-swine IL-13. After washing, membrane was probed with Dylight(TM) 649 Conjugated Anti-Rabbit IgG (H&L) (Donkey) Antibody Rabbit IgG Aff - Purified Por E, WB
0.1 mg / €450.00
  Acris Antibodies GmbH

Interleukin-13 / IL13 antibody

Western blot using Protein-A Purified Anti-swine IL-13 antibody shows detection of recombinant swine IL-13 at 13.2kDa (arrow) raised in yeast. Multiple bands are expected of the glycosylated protein. Protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA (BSA-30, diluted 1:10), and probed with Rockland Immunochemicals, Inc. Anti-swine IL-13. After washing, membrane was probed with Dylight(TM) 649 Conjugated Anti-Rabbit IgG (H&L) (Donkey) Antibody Rabbit IgG Aff - Purified Por E, WB
25 µl / €190.00
  Acris Antibodies GmbH

Interleukin-13 / IL13 antibody

Interleukin-13 / IL13 Rabbit Biotin Hu E, WB
25 µg / €230.00
  Acris Antibodies GmbH

Interleukin-13 / IL13 antibody

Interleukin-13 / IL13 Rabbit Biotin Hu E, WB
50 µg / €340.00
  Acris Antibodies GmbH

Items 1 to 20 of 26 total

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