Interleukin-15 / IL15 antibody

Principal name

Interleukin-15 / IL15 antibody

Alternative names for Interleukin-15 / IL15 antibody

IL-15

SwissProt ID

O97687 (Felca), P40933 (Human), P48346 (Mouse), P97604 (Rat), Q28028 (Bovin), Q3Y5G8 (Rabit), Q75SZ9 (Cavpo), Q95253 (Pig), Q9XSJ6 (Sheep)

Gene ID

3600 (IL15), 16168 (Il15), 25670

Available reactivities

Hu (Human), Rt (Rat), Ms (Mouse), Bov (Bovine), Can (Canine), Por (Porcine), Rb (Rabbit), Eq (Equine), GP (Guinea Pig), Sh (Sheep)

Available hosts

Mouse, Rabbit, Sheep, Rat, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Functional assay (FN), Paraffin Sections (P), Immunoprecipitation (IP), Neutralisation (NEUT), Frozen Sections (C), ELISA (detection) (E(detection)), EPair for Elisa (EPair)

Background of Interleukin-15 / IL15 antibody

IL15 (114 amino acids) is a cytokine that regulates T and natural killer cell activation and proliferation. It has a predicted molecular mass of approximately 12.5 kDa. Human IL15 shares approximately 97% and 73% amino acid sequence identity with simian and mouse IL15, respectively. Both human and simian IL15 are active on mouse cells. IL15 was initially isolated from the simian kidney epithelial cell line CV1/EBNA. It has also been isolated from mouse and human cell sources. The cytokines IL15 and IL2 share many biological properties and stimulatory activities (T, B, and NK cells). Both IL15 and IL2 stimulate mouse CTLL2 cells. In activated peripheral blood T lymphocytes, IL2 is highly expressed but the expression of IL15 is not detectable. There is no sequence homology between IL15 and IL2, though computer modeling indicates both possess a four alpha helical bundle structure. IL15 competes for binding sites with IL2, as both IL2 and IL15 stimulate the growth of cells through the IL2 receptor. IL15 mRNA is expressed in many cell types and tissues including adherent peripheral blood mononuclear cells, fibroblasts, and epithelial cells, monocytes, placenta, and skeletal muscle.
IL-15 (14-15 kD) is a member of the four alpha-helical bundle family of cytokines. It is very similar to IL-2, except that IL-15 has an IL-15 alpha receptor subunit. IL-15 plays an important role in the growth and differentiation of T and B lymphocytes, natural killer cells, macrophages, and monocytes as well as activation of a number of important intracellular signaling molecules. This implies that IL-15 could be essential for the immune responses, allograft rejection, and the pathogenesis of autoimmune diseases.

General readings

Ozawa A., et al. (2009). J immunol. 183(3):2193.
Olsen SK., et al. (2007). J Biol Chem. 282(51):37191-204.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Interleukin-15 / IL15 (70-82) antibody

Staining of Human Bone Marrow lysate using Interleukin-15 / IL15 antibody Cat.-No  AP32152PU-N at 1 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence Goat Aff - Purified Hu WB
0.1 mg / €340.00
  Acris Antibodies GmbH

Interleukin-15 / IL15 antibody

Western blot analysis of Mouse IL-15 in transfected supernatant. Rabbit Purified Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-15 / IL15 antibody

Western blot analysis of IL-15 in rat spleen tissue lysate with IL-15 antibody at (A) 1 and (B) 2 ug/mL Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-15 / IL15 antibody

To detect hIL-15 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-15. Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Interleukin-15 / IL15 antibody

To detect hIL-15 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-15 (TA328232) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-15. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-15 / IL15 antibody

To detect mIL-15 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant mIL-15. Rabbit IgG Purified E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Interleukin-15 / IL15 antibody

To detect mIL-15 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml.  Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-15 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Rabbit IgG Purified E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-15 / IL15 antibody

WB Suggested Anti-IL15 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: 293T cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Sh WB
50 µg / €325.00
  OriGene Technologies, Inc.

Interleukin-15 / IL15 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL15 (RC219294, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL15.(1:500) Mouse IgG1 OTI2B4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Interleukin-15 / IL15 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL15 (RC219294, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL15.(1:500) Mouse IgG2a OTI3D4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Interleukin-15 / IL15 antibody

Interleukin-15 / IL15 Mouse IgG1 BDI150 Purified Hu C, E, IP, WB
0.5 mg / €360.00
  Acris Antibodies GmbH

Interleukin-15 / IL15 antibody

Neutralizing activity with anti-Mouse IL-15 monoclonal antibody (AM26520AF-N). After CTLL-2 cells are added AM26520AF-N and incubated for 1 hour, IL-15 (A) or IL-2 (B) are added and incubated overnight. Subsequently 1.0 µCi 3H-Methyl-Thymidine are added and incubated for 6-8 hours. After CTLL-2 cells are harvested, The radioactivity is counted with the liquid scintillation counter. The inhibition rate (%) is shown in the vertical axis and the concentration of AM26520AF-N is shown in the horizontal axis.These data were kindly provided by Dr. Kazuto Ishida, Ph.D. and Professor Dr. Toshiaki Ohteki, Ph.D. (Department of Immunology, Akita University School of Medicine) Rat IgG2a AIO.3 Azide Free Ms FN
0.1 mg / €340.00
  Acris Antibodies GmbH

Interleukin-15 / IL15 antibody

Interleukin-15 / IL15 Mouse IgG1 B-E29 Azide Free Hu FN
0.5 mg / €460.00
  Acris Antibodies GmbH

Interleukin-15 / IL15 antibody

Interleukin-15 / IL15 Rabbit Biotin Ms E, WB
50 µg / €340.00
  Acris Antibodies GmbH

Interleukin-15 / IL15 antibody

Interleukin-15 / IL15 Rabbit Biotin Ms E, WB
25 µg / €230.00
  Acris Antibodies GmbH

Interleukin-15 / IL15 antibody

Interleukin-15 / IL15 Rabbit Aff - Purified Ms E, FN, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

Interleukin-15 / IL15 antibody

Interleukin-15 / IL15 Rabbit Aff - Purified Ms E, FN, WB
50 µg / €230.00
  Acris Antibodies GmbH

Interleukin-15 / IL15 antibody

Interleukin-15 / IL15 Mouse IgG1 Azide Free Hu E, WB
0.5 mg / €220.00
  Acris Antibodies GmbH

Interleukin-15 / IL15 antibody

Direct ELISA using Interleukin-15 / IL15 Antibody Cat,.-No PP1035B Rabbit Biotin Hu E, WB
25 µg / €230.00
  Acris Antibodies GmbH

Interleukin-15 / IL15 antibody

Direct ELISA using Interleukin-15 / IL15 Antibody Cat,.-No PP1035B Rabbit Biotin Hu E, WB
50 µg / €340.00
  Acris Antibodies GmbH

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