Interleukin-17A (IL17A) antibody

Principal name

Interleukin-17A (IL17A) antibody

Alternative names for Interleukin-17A (IL17A) antibody

IL-17A, IL17, IL-17, CTLA8, CTLA-8, Cytotoxic T-lymphocyte-associated antigen 8

SwissProt ID

Q16552 (Human), Q60I29 (Pig), Q61453 (Rat), Q62386 (Mouse), Q687Y7 (Bovin)

Gene ID

3605 (IL17A), 16171 (Il17a), 301289

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine)

Available hosts

Rabbit, Rat, Goat, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Functional assay (FN), Paraffin Sections (P), Flow Cytometry (F), Neutralisation (NEUT), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), ELISA (capture) (E(capture)), ELISA (detection) (E(detection)), ELISA capture and detection (E(caption)/E(detection)), Luminex (LMNX)

Background of Interleukin-17A (IL17A) antibody

Mouse Interleukin 17 (IL-17; also known as IL-17A and CTLA-8) is a 21 kDa, variably glycosylated polypeptide that belongs to the IL-17 family of cytokines containing a cysteine-knot fold . Its sequence was originally isolated from an activated hybridoma created from the fusion of a mouse cytotoxic and rat T cell lymphoma cell line . It is synthesized as a 158 amino acid (aa) precursor that contains a 25 aa signal sequence and a 15 kDa, 133 aa mature segment . In both mouse and human, there is one conserved N-linked glycosylation site that likely contributes 5 kDa to its native molecular weight. IL-17A forms both a 35-38 kDa homodimer,and a 45-48 kDa heterodimer with IL-17F . Mature mouse IL-17A is 61% and 89% aa identical to human and rat IL-17A, respectively . While rodent and human mature sequences show modest aa sequence identity, human IL-17 is active on both mouse and rat cells . Cells known to produce IL-17 are the CD4+ Th17 T cells, Paneth cells, GR1+CD11b+ myeloid suppressor cells, CD27-.. T cells, CD1+NK1.1- iNKT cells and CD3-CD4+ LTi-like cells(3, 5, 6, 10-12).

A high affinity receptor for mouse IL-17 has been reported, and appears to be a heteromultimer of IL-17RA and IL-17RC, likely in a 2:1 ratio . IL-17RA is a 130 kDa, type I transmembrane glycoprotein that bears no resemblance to members of the cytokine, TNF or immunoglobulin receptor superfamily . IL-17RC is also a type I transmembrane protein, approximately 90-95 kDa in size, that shares less than 30% aa identity with IL-17RA . Both receptors are needed for IL-17A and IL-17A/F activity. The two receptors appear to form a functional association following ligand binding to IL-17RA .

IL-17 is best known for its participation in the recruitment and survival of neutrophils . Its induction was initially described to be the result of antigen stimulation of dentritic cells, resulting in IL-23 secretion. In a TCR-independent event, IL-23 induces T cell production of IL-17. Once secreted, IL-17 in the bone marrow would seem to induce stromal/fibroblast expression of both G-CSF and SCF (membrane form), an effect that increases neutrophil differentiation and activation. IL-17 may complement this by directly blocking neutrophil apoptosis, promoting greater circulating neutrophil numbers . In the tissues, IL-17 seems to promote neutrophil extravasation, principally through its effects on macrophages and endothelial cells (EC). On macrophages, IL-17 induces TNF-., IL-1. and IL-6 production .TNF-. and IL-1. then act on local ECs to induce G-CSF secretion, an effect that is potentiated by IL-17 . IL-17 further contributes to neutrophil influx by inducing EC CXC chemokine release and NO production, which may increase vascular permeability . IL-17 effects are not limited to neutrophils. In synovial joints, IL-17 upregulates RANKL expression on osteoblasts. This provides a stimulus for osteoclast formation and subsequent bone resorption .

General readings

Gaffen, S. (2009) Nat. Rev. Immunol. 9:556.

Iwakura, Y. et al. (2008) Immunol. Rev. 226:57.

Kolls, J.K. and A. Linden (2004) Immunity 21:467.

Yao, Z. et al. (1995) J. Immunol. 155:5483.

Kennedy, J. et al. (1996) J. Interf. Cytokine Res. 16:611.

Liang, S.C. et al. (2007) J. Immunol. 179:7791.

Chang, S. and C. Dong (2007) Cell Res. 17:435.

Rouvier, E. et al. (1993) J. Immunol. 150:5445.

Miljkovic, D.J. Et al. (2003) Cell. Mol. Life Sci. 60:518.

Witowski, J. et al. (2004) Cell. Mol. Life Sci. 61:567.

Cua, D.J. and C.M. Tato (2010) Nat. Rev. Immunol. 10:479..

Shin, H.C. et al. (1998) Cytokine 10:841.

Yao, Z. et al. (1995) Immunity 3:811.

Haudenschild, D. et al. (2002) J. Biol. Chem. 277:4309.

Toy, D. et al. (2006) J. Immunol. 177:36.

Hu, Y. et al. (2010) J. Immunol. 184:4307.

Schwarzenberger, P. et al. (2000) J. Immunol. 164:4783.

Yu, J.J. and S.L. Gaffen (2008) Front. Biosci. 13:170.

Jovanovic, D.V. et al. (1998) J. Immunol. 160:3513.

Numasaki, M. et al. (2004) Immunol. Lett. 95:97.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Interleukin-17A (IL17A) antibody

AP32038PU-N Interleukin-17A Antibody staining of Human Thymus lysate at 1 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence Goat Aff - Purified Hu WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Interleukin-17A (IL17A) (N-term) antibody

  Rabbit Polyclonal IL-17 Antibody  
Western blot analysis of IL-17 in A-20 lysate with IL-17 antibody at (A) 2 and (B) 4 µg/ml. Rabbit IgG Aff - Purified Hu, Ms E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-17A (IL17A) (Center) antibody

  Rabbit Polyclonal IL-17 Antibody  
Western blot analysis of IL-17 in A-20 lysate with IL-17 antibody at (A) 0.5 and (B) 1 µg/ml. Rabbit IgG Aff - Purified Hu, Ms E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-17A (IL17A) (126-137) antibody

  Goat Anti-IL-17A (aa126-137) Antibody  
TA311224 (1ug/ml) staining of Human Lymph Node lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-17A (IL17A) antibody

  IL-17 Mouse Monoclonal (aa1-75) (4K5F6) Antibody  
Anti-IL-17 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5-10 ug/ml. Mouse IgG2b 4K5F6 Purified Hu
50 µg / €400.00
  OriGene Technologies, Inc.

Interleukin-17A (IL17A) antibody

  Rabbit polyclonal anti-IL-17A antibody  
WB using anti-Human IL17-A antibody shows detection of a band ~17 kDa in size corresponding to recombinant human IL17-A (lane 1). Molecular weight markers are also shown (M). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution. Detection occurred using DyLight 649 conjugated anti-Rabbit IgG secondary antibody diluted 1:20,000 in blocking buffer. Rabbit IgG Purified Hu E, NEUT, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

Interleukin-17A (IL17A) antibody

  Rabbit polyclonal IL-17A antibody Biotin Conjugated  
WB using anti-Human IL17-A antibody shows detection of a band ~17 kDa in size corresponding to recombinant human IL17-A (lane 1). Molecular weight markers are also shown (M). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution. Detection occurred using DyLight 649 conjugated anti-Rabbit IgG (p/n 611-143-122) secondary antibody diluted 1:20,000 in blocking buffer (p/n MB-070). Rabbit IgG Purified Hu E, P, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

Interleukin-17A (IL17A) antibody

  Rabbit polyclonal anti-IL-17A antibody  
Western blot using anti-IL-17A antibody shows detection of rat recombinant IL-17A protein (lane 1). Approximately 2 µg of recombinant protein was loaded onto the gel. Primary antibody was used at a 1:1,000 dilution. The membrane was washed and reacted with a 1:20,000 dilution of DyLight™ 649 conjugated Gt-a-Rabbit IgG (p/n 611-143-122). Molecular weight estimation was made by comparison to prestained MW markers indicated at the left. Other detection systems will yield similar results. Rabbit IgG Ig Fraction Ms, Rt E, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

Interleukin-17A (IL17A) antibody

  Rabbit polyclonal IL-17A antibody Biotin Conjugated  
WB showing detection of Rat IL-17A. 50ng of Rat IL-17A (Lane 1) was run on a 4-20% gel and transferred to 0.45 µm nitrocellulose. Anti-Rat IL-17A (RABBIT) Antibody Biotin Conjugated  secondary antibody was used at 1:5000 in Blocking Buffer for Fluorescent WBting (p/n MB-070). HRP Streptavidin (p/n S000-03) was used at 1:40,000 in MB-070 for 30 min at 20°C and imaged using the Bio-Rad VersaDoc® 4000 MP. Arrow indicates correct 15 kDa molecular weight position expected for Rat IL-17A. Rabbit IgG Purified Ms, Rt E, P, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

Interleukin-17A (IL17A) antibody

  Mouse monoclonal IL-17A antibody  
Western Blot of Mouse Anti-IL-17A antibody Lane 1: human full length recombinant IL-17A protein Lane 2: mouse full length recombinant IL-17A protein Lane 3: rat full length recombinant IL-17A protein Load: 20 ng/lane Primary antibody: Anti-IL-17A antibody at 1ug/mL for overnight at 4°C Mouse 4H1524.1 Purified Hu F, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

Interleukin-17A (IL17A) antibody

  Mouse monoclonal Anti-Human IL-17A (MOUSE) Biotin Conjugated antibody  
Western Blot of Mouse Anti-IL-17A antibody Lane 1: human full length recombinant IL-17A protein Lane 2: mouse full length recombinant IL-17A protein Lane 3: rat full length recombinant IL-17A protein Load: 20 ng/lane Primary antibody: Anti-IL-17A antibody at 1ug/mL for overnight at 4°C Mouse 4H1524.1 Purified Hu F, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

Interleukin-17A (IL17A) antibody

  Rat monoclonal Mouse IL-17A antibody  
WB showing detection of Mouse IL-17A. 100 ng of Mouse IL-17A was run on a 4-20% gel and transferred to 0.45 µm nitrocellulose.  Anti-Mouse IL-17A (RAT) Antibody (p/n TA319587) was used at 1:1000. Peroxidase conjugated Rabbit Anti-mouse secondary antibody  was diluted at 1:40,000 for 30 min at 20°C and imaged using the Bio-Rad VersaDoc® 4000 MP. Band indicates correct 23 kDa molecular weight position expected for Mouse IL-17A. Rat 20B4.G10.F5 Purified Ms E, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

Interleukin-17A (IL17A) antibody

  Biotinylated Anti-Human IL-17 (IL-17A) Rabbit Polyclonal Antibody  
To detect hIL-17A by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-17A. Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Interleukin-17A (IL17A) antibody

  Biotinylated Anti-Human IL-17 (IL-17A) Goat Polyclonal Antibody  
To detect hIL-17A by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-17A. Goat IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Interleukin-17A (IL17A) antibody

  Anti-Human IL-17 (IL-17A) Goat Polyclonal Antibody  
To detect hIL-17A by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-17A (TA328237) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-17A. Goat IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-17A (IL17A) antibody

  Anti-Human IL-17 (IL-17A) Rabbit Polyclonal Antibody  
To detect hIL-17A by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-17A (TA328236) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-17A. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-17A (IL17A) antibody

  Biotinylated Anti-Murine IL-17A Rabbit Polyclonal Antibody  
To detect mIL-17A by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant mIL-17A. Rabbit IgG Purified E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Interleukin-17A (IL17A) antibody

  Anti-Murine IL-17A Rabbit Polyclonal Antibody  
To detect mIL-17A by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine IL-17A (TA328502) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-17A. Rabbit IgG Purified E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-17A (IL17A) antibody

  Mouse Monoclonal IL-17/IL-17A Antibody (4k5F6)  
Western Blot: IL17 Antibody (4k5F6) [TA337063] - Analysis of 1) human, 2) mouse and 3) rat full-length recombinant IL-17A protein using IL-17A antibody at 0.2 ug/ml. Mouse IgG2a 4K5F6 Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-17A (IL17A) antibody

  ELISA and Luminex validated lL-17 Mouse monoclonal antibody clone (4C1, clone OTI4C17 (formerly 4C17)  
lL-17 ELISA with 4C17 Capture (TA600015) and 4D17 Detection (TA700015) Antibodies. Substrate used: Recombinant Human lL-17 (E. Coli expressed Peptide) Mouse IgG1 OTI4C17 Purified Hu E, LMNX
0.1 mg / €325.00
  OriGene Technologies, Inc.

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