Interleukin-17A (IL17A) antibody

Principal name

Interleukin-17A (IL17A) antibody

Alternative names for Interleukin-17A (IL17A) antibody

IL-17A, IL17, IL-17, CTLA8, CTLA-8, Cytotoxic T-lymphocyte-associated antigen 8

SwissProt ID

Q16552 (Human), Q60I29 (Pig), Q61453 (Rat), Q62386 (Mouse), Q687Y7 (Bovin)

Gene ID

3605 (IL17A), 16171 (Il17a), 301289

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine)

Available hosts

Rabbit, Rat, Goat, Chicken, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Functional assay (FN), Paraffin Sections (P), Flow Cytometry (F), Neutralisation (NEUT), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), ELISA (capture) (E(capture)), ELISA (detection) (E(detection)), ELISA capture and detection (E(caption)/E(detection)), Luminex (LMNX)

Background of Interleukin-17A (IL17A) antibody

The primary function of the body defense mechanism/immune system is to protect the organism from invading pathogens. In this regard a very effective and dynamic cellular network has evolved that comprise of innate and adaptive immunity. Most components of innate immunity are present before the onset of infection and constitute cellular and molecular components that recognize classes of molecules peculiar to pathogens. In contrast, adaptive immunity responds to the challenge with a high degree of specificity as well as the remarkable property of memory. Although adaptive immune responses are the most effective to clear invading pathogens (1, 2), innate immune responses should be precedent of activation of adaptive immune cells (2). In other words, adaptive immune responses can not arise without help of innate immune responses. Antigen presenting cells (APCs) are key initiators of immune responses. During infection various cytochines are released and via activation of various TLR receptor family, they synthesized various interleukins. IL17 gene was mapped at a single site on mouse chromosome 1A and human chromosome 2q31 (2).
Pro inflammatory cytokine, IL-17 (IL-17A or CTLA-8) plays a central role in inflammation and autoimmunity. The iIL17, like other cytokines is produce by activation of T cell receptor through CD3 cross linking (1). IL-17 is a crucial effector cytokine, whose production is specifically triggered by IL-23, and it has been shown to be an essential inflammatory mediator in other autoimmune diseases and inflammatory conditions. In human the co-stimulation of T cells thru CD28 several costimulatory molecules (ICOS, 4-1BB, CD40L) mildly enhance the IL17 expression where as IL23 profoundly enhance the CD3 induced IL17 expression. The IL-17 expression is also sensitive to cyclosporin-A and MAPK inhibitors, suggesting the involvement of the calcineurin/NFAT and MAPK signaling pathways.
The predicted 155-amino acids sequence contains an N-terminal signal peptide and exhibits 72% amino acid identity with HVS13, an open reading frame from a T-lymphotropic Herpesvirus saimiri, and 63% with murine CTLA8 (3). Based on hydropathy plot has 2 transmembrane domains (TMD) at N and C-terminal end of the protein. The anti-IL17 antibodies were made against an epitope that lies near the extracytoplasmic domain on IL17 protien.

General readings

1. Fossiez F, etl. Al., T cell interleukin-17 induces stromal cells to produce proinflammatory and hematopoietic cytokines. J Exp Med. 1996 Jun 1;183(6):2593-603.
2. Rouvier E, Luciani MF, Mattei MG, Denizot F, Golstein P. CTLA-8, cloned from an activated
T cell, bearing AU-rich messenger RNA instability sequences, and homologous to a herpesvirus saimiri gene. J Immunol. 1993 Jun 15;150(12):5445-56.
3. Yao Z., et. Al., Human IL-17: a novel cytokine derived from T cells. J Immunol. 1995 Dec 15;155(12):5483-6.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Interleukin-17A (IL17A) antibody

AP32038PU-N Interleukin-17A Antibody staining of Human Thymus lysate at 1 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence Goat Aff - Purified Hu WB
0.1 mg / €340.00
  Acris Antibodies GmbH

Interleukin-17A (IL17A) (N-term) antibody

Western blot analysis of IL-17 in A-20 lysate with IL-17 antibody at (A) 2 and (B) 4 µg/ml. Rabbit IgG Aff - Purified Hu, Ms E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-17A (IL17A) (Center) antibody

Western blot analysis of IL-17 in A-20 lysate with IL-17 antibody at (A) 0.5 and (B) 1 µg/ml. Rabbit IgG Aff - Purified Hu, Ms E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-17A (IL17A) (126-137) antibody

TA311224 (1ug/ml) staining of Human Lymph Node lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-17A (IL17A) antibody

Anti-IL-17 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5-10 ug/ml. Mouse IgG2b 4K5F6 Purified Hu
50 µg / €345.00
  OriGene Technologies, Inc.

Interleukin-17A (IL17A) antibody

WB using anti-Human IL17-A antibody shows detection of a band ~17 kDa in size corresponding to recombinant human IL17-A (lane 1). Molecular weight markers are also shown (M). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution. Detection occurred using DyLight 649 conjugated anti-Rabbit IgG secondary antibody diluted 1:20,000 in blocking buffer. Rabbit IgG Purified Hu E, NEUT, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-17A (IL17A) antibody

WB using anti-Human IL17-A antibody shows detection of a band ~17 kDa in size corresponding to recombinant human IL17-A (lane 1). Molecular weight markers are also shown (M). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution. Detection occurred using DyLight 649 conjugated anti-Rabbit IgG (p/n 611-143-122) secondary antibody diluted 1:20,000 in blocking buffer (p/n MB-070). Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-17A (IL17A) antibody

Western blot using anti-IL-17A antibody shows detection of rat recombinant IL-17A protein (lane 1). Approximately 2 µg of recombinant protein was loaded onto the gel. Primary antibody was used at a 1:1,000 dilution. The membrane was washed and reacted with a 1:20,000 dilution of DyLight™ 649 conjugated Gt-a-Rabbit IgG (p/n 611-143-122). Molecular weight estimation was made by comparison to prestained MW markers indicated at the left. Other detection systems will yield similar results. Rabbit IgG Ig Fraction Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-17A (IL17A) antibody

WB showing detection of Rat IL-17A. 50ng of Rat IL-17A (Lane 1) was run on a 4-20% gel and transferred to 0.45 µm nitrocellulose. Anti-Rat IL-17A (RABBIT) Antibody Biotin Conjugated  secondary antibody was used at 1:5000 in Blocking Buffer for Fluorescent WBting (p/n MB-070). HRP Streptavidin (p/n S000-03) was used at 1:40,000 in MB-070 for 30 min at 20°C and imaged using the Bio-Rad VersaDoc® 4000 MP. Arrow indicates correct 15 kDa molecular weight position expected for Rat IL-17A. Rabbit IgG Purified Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-17A (IL17A) antibody

Western Blot of Mouse Anti-IL-17A antibody Lane 1: human full length recombinant IL-17A protein Lane 2: mouse full length recombinant IL-17A protein Lane 3: rat full length recombinant IL-17A protein Load: 20 ng/lane Primary antibody: Anti-IL-17A antibody at 1ug/mL for overnight at 4°C Mouse 4H1524.1 Purified Hu F, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-17A (IL17A) antibody

Western Blot of Mouse Anti-IL-17A antibody Lane 1: human full length recombinant IL-17A protein Lane 2: mouse full length recombinant IL-17A protein Lane 3: rat full length recombinant IL-17A protein Load: 20 ng/lane Primary antibody: Anti-IL-17A antibody at 1ug/mL for overnight at 4°C Mouse 4H1524.1 Purified Hu F, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-17A (IL17A) antibody

WB showing detection of Mouse IL-17A. 100 ng of Mouse IL-17A was run on a 4-20% gel and transferred to 0.45 µm nitrocellulose.  Anti-Mouse IL-17A (RAT) Antibody (p/n TA319587) was used at 1:1000. Peroxidase conjugated Rabbit Anti-mouse secondary antibody  was diluted at 1:40,000 for 30 min at 20°C and imaged using the Bio-Rad VersaDoc® 4000 MP. Band indicates correct 23 kDa molecular weight position expected for Mouse IL-17A. Rat 20B4.G10.F5 Purified Ms E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-17A (IL17A) antibody

To detect hIL-17A by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-17A. Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Interleukin-17A (IL17A) antibody

To detect hIL-17A by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-17A. Goat IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Interleukin-17A (IL17A) antibody

To detect hIL-17A by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-17A (TA328237) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-17A. Goat IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-17A (IL17A) antibody

To detect hIL-17A by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-17A (TA328236) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-17A. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-17A (IL17A) antibody

To detect mIL-17A by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant mIL-17A. Rabbit IgG Purified E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Interleukin-17A (IL17A) antibody

To detect mIL-17A by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine IL-17A (TA328502) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-17A. Rabbit IgG Purified E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-17A (IL17A) antibody

Western Blot: IL17 Antibody (4k5F6) [TA337063] - Analysis of 1) human, 2) mouse and 3) rat full-length recombinant IL-17A protein using IL-17A antibody at 0.2 ug/ml. Mouse IgG2a 4K5F6 Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-17A (IL17A) antibody

lL-17 ELISA with 4C17 Capture (TA600015) and 4D17 Detection (TA700015) Antibodies. Substrate used: Recombinant Human lL-17 (E. Coli expressed Peptide) Mouse IgG1 OTI4C17 Purified Hu E, LMNX
0.1 mg / €325.00
  OriGene Technologies, Inc.

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