Interleukin-17F / IL17F antibody

Principal name

Interleukin-17F / IL17F antibody

Alternative names for Interleukin-17F / IL17F antibody

IL24, Cytokine ML-1, Interleukin-24, IL-24

SwissProt ID

Q5BJ95 (Rat), Q6NSI0 (Human), Q7TNI7 (Mouse), Q96PD4 (Human)

Gene ID

112744 (IL17F), 257630 (Il17f), 301291

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), EPair for Elisa (EPair), Immunoprecipitation (IP), Functional assay (FN), ELISA (capture) (E(capture)), Flow Cytometry (F), Neutralisation (NEUT), Frozen Sections (C)

Background of Interleukin-17F / IL17F antibody

The monoclol antibody H17F10A7 reacts with human IL-17F, a member of the IL-17 family of pro-inflammatory cytokines. Like IL-17A, IL-17F is a disulfide-linked, homodimeric glycoprotein. The IL-17F homodimer includes a classical cysteine knot motif, which is found also in the TGF-?, BMP, and NGF superfamilies. The presence of the cysteine knot motif suggested the possibility of a heterodimeric structure, as was reported for TGF-? and inhibictivin. Recent reports confirm that co-expression of IL-17F and IL-17A in HEK293 cells results in the formation of biologically active IL-17F/IL-17A heterodimers, in addition to the IL-17F homodimers and IL-17A homodimers. Moreover, activated human CD4+ T cells were found to produce the IL-17A/F heterodimer, along with the corresponding homodimers. In comparing the relative potency of IL-17A, IL-17F, and IL-17A/F, all three were found to induce GRO-a secretion; IL-17A was most potent, followed by IL-17A/F heterodimer, then IL-17F (100fold lower than IL-17A). These heterodimers can be detected by immunoprecipitation with eBio64CAP17 anti-IL-17A monoclol antibody followed by immunoblot with H17F10A7.

General readings

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Shu, Q., et al. Hum. Immunol. 71(10):988-991(2010)
Paradowska-Gorycka, A., et al. Scand. J. Immunol. 72(2):134-141(2010)
Tanasescu, C., et al. Eur. J. Intern. Med. 21(3):202-207(2010)
Davila, S., et al. Genes Immun. 11(3):232-238(2010)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Interleukin-17F / IL17F antibody

  Rabbit polyclonal anti-IL-17F antibody  
WB using anti-Human IL17-F antibody shows detection of a band ~15 kDa in size corresponding to recombinant human IL17-F (lane 1). Molecular weight markers are also shown (M). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution. Detection occurred using DyLight 649 conjugated anti-Rabbit IgG (p/n 611-143-122) secondary antibody diluted 1:20,000 in blocking buffer (p/n MB-070). Rabbit IgG Purified Hu E, NEUT, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

Interleukin-17F / IL17F antibody

  Mouse Monoclonal IL-17F Antibody (4H1629)  
Western Blot: IL17F Antibody (4H1629) [TA337155] - WB analysis on full-length recombinant IL-17F proteins of (1) Human, (2) Mouse and (3) Rat using IL17F antibody (clone 4H1629) at a concentration of 0.5 ug/ml. Note: This antibody did not detect the mouse Mouse 4H1629 Purified Hu C, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-17F / IL17F antibody

  Rabbit polyclonal IL-17F antibody Peroxidase Conjugated  
Western Blot of Peroxidase conjugated Rabbit anti-IL-17F Antibody. Lane 1: Human IL-17F. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Peroxidase IL-17F secondary antibody at 1:1,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 16kDa for Human IL-17F. Other band(s): none. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

Interleukin-17F / IL17F antibody

  Rabbit polyclonal anti-IL-17F antibody  
WB using anti-Mouse IL-17F antibody shows detection of a band ~18 kDa corresponding to recombinant mouse IL-17F.  Primary antibody was used at 1:1,000 dilution. Rabbit IgG Purified Ms, Rt E, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

Interleukin-17F / IL17F antibody

  Mouse monoclonal IL-17F antibody  
Western Blot of Mouse Anti-IL-17F antibody Lane 1: human full length recombinant IL-17F protein Lane 2: mouse full length recombinant IL-17F protein Lane 3: rat full length recombinant IL-17F protein Load: 20 ng/lane Primary antibody: Anti-IL-17F antibody at 0.2ug/mL for overnight at 4°C Mouse 4H450.1 Purified Hu WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

Interleukin-17F / IL17F antibody

  Mouse monoclonal Anti-Human IL-17F (MOUSE) Biotin Conjugated antibody  
Western Blot of Mouse Anti-IL-17F antibody Lane 1: human full length recombinant IL-17F protein Lane 2: mouse full length recombinant IL-17F protein Lane 3: rat full length recombinant IL-17F protein Load: 20 ng/lane Primary antibody: Anti-IL-17F antibody at 0.2ug/mL for overnight at 4°C Mouse 4H450.1 Purified Hu WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

Interleukin-17F / IL17F antibody

  Mouse monoclonal Anti-Human IL-17F Biotin Conjugated antibody  
Western Blot of Mouse Anti-IL-17F antibody Lane 1: human full length recombinant IL-17F protein Lane 2: mouse full length recombinant IL-17F protein Lane 3: rat full length recombinant IL-17F protein Load: 20 ng/lane Primary antibody: Anti-IL-17F antibody at 0.5ug/mL for overnight at 4°C Mouse 4H1629.1 Purified Hu WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

Interleukin-17F / IL17F antibody

  Mouse monoclonal IL-17F antibody  
Western Blot of Mouse Anti-IL-17F antibody Lane 1: human full length recombinant IL-17F protein Lane 2: mouse full length recombinant IL-17F protein Lane 3: rat full length recombinant IL-17F protein Load: 20 ng/lane Primary antibody: Anti-IL-17F antibody at 0.5ug/mL for overnight at 4°C Mouse 4H1629.1 Purified Hu WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

Interleukin-17F / IL17F antibody

  Mouse Anti-Human IL-17F Purified (25 ug)  
IL-17 producing cells were generated by culturing human PBMC with anti-Human CD3 and anti-Human CD28 or PMA and Ionomycin in the presence of Brefeldin A (2) for 16 hours.  Cell lysates were run under reducing conditions, immunoblotted with 2 ug/ml of Anti-Human IL-17F Purified and revealed with Anti-Mouse HRP. Mouse H17F10A7 Purified Hu WB
25 µg / €150.00
  OriGene Technologies, Inc.

Interleukin-17F / IL17F antibody

  Biotinylated Anti-Human IL-17F Rabbit Polyclonal Antibody  
To detect hIL-17F by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-17F. Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Interleukin-17F / IL17F antibody

  Anti-Human IL-17F Rabbit Polyclonal Antibody  
To detect hIL-17F by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-17F (TA328242) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-17F. Rabbit IgG Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-17F / IL17F antibody

Interleukin-17F / IL17F Mouse IgG2b B-F60 Azide Free Hu FN
0.5 mg / €460.00
  Acris Antibodies GmbH

Interleukin-17F / IL17F antibody

Interleukin-17F / IL17F Mouse IgG2b B-G46 Azide Free Hu E(capture), Elispot
1 mg / €790.00
  Acris Antibodies GmbH

Interleukin-17F / IL17F antibody

Interleukin-17F / IL17F Mouse IgG2b B-F60 Biotin Hu E(detection), Elispot
0.1 mg / €460.00
  Acris Antibodies GmbH

Interleukin-17F / IL17F antibody

The graph on the right represents activated TH17 cells (5h by PMA-ionomycin) marked with B-C52 and the graph on the left shows B-C52 with non-activated TH17 cells. In both cases, the dotted line represents the IgG1 Isotype Control (B-Z1) Mouse IgG1 B-C52 Azide Free Hu F
0.5 mg / €460.00
  Acris Antibodies GmbH

Interleukin-17F / IL17F antibody

The graph on the right represents activated TH17 cells (5h by PMA-ionomycin) marked with B-C52 and the graph on the left shows B-C52 with non-activated TH17 cells. In both cases, the dotted line represents the IgG1 Isotype Control (B-Z1) Mouse IgG1 B-C52 Purified Hu F
2 ml/200Tests / €250.00
  Acris Antibodies GmbH

Interleukin-17F / IL17F (N-term) antibody

Western blot analysis of IL17F (arrow) using Interleukin-17F / IL17F Antibody (N-term) Cat.-No AP52188PU-N. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the IL17F gene (Lane 2) . Rabbit Ig Aff - Purified Hu F, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

IL17F - human gene knockout kit via CRISPR

IL17F - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

IL17F - human gene knockout kit via CRISPR

IL17F - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

IL17F - human gene knockout kit via CRISPR

IL17F - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

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