Interleukin-17F / IL17F antibody

Principal name

Interleukin-17F / IL17F antibody

Alternative names for Interleukin-17F / IL17F antibody

IL24, Cytokine ML-1, Interleukin-24, IL-24

SwissProt ID

Q5BJ95 (Rat), Q6NSI0 (Human), Q7TNI7 (Mouse), Q96PD4 (Human)

Gene ID

112744 (IL17F), 257630, 301291

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Neutralisation (NEUT), EPair for Elisa (EPair), Immunoprecipitation (IP), Functional assay (FN), ELISA (capture) (E(capture)), Flow Cytometry (F), Frozen Sections (C)

Background of Interleukin-17F / IL17F antibody

Interleukin-17 (IL-17) is a 155 amino acid protein that is a disulfide linked, homodimeric, secreted glycoprotein with one N-linked glycosylation site. IL-17 is also referred to as IL- 17A. Other members include IL-17B, IL-17C, IL-17D, IL-17E and IL-17F. IL-17 binds to the cell surfacer receptor IL-17R. IL-17 is secreted by a subset of T cells called T-helper 17 (Th-17) cells. IL-23 is thought to mediate the production of IL-17 by Th-17 cells. IL-17 has an immune regulatory function, mediating pro-inflammatory responses, including the induction of other cytokines including IL-6, G-CSF, GM-CSF and TNF- and chemokines such as IL-8, MCP-1 and GRO-. The increased expression of these cytokines and chemokines attract other cells including neutrophils. IL-17 therefore plays a role in inflammatory diseases such as asthma, rheumatoid arthritis and also psoriasis. This is a HCX protein. HCX Expression System Details HCX proteins mimic the proteins in the human body because they are expressed from human, rather than animal, insect or bacterial cells. This process gives them human post-translational modifications. Recombinant DNA techniques allow a human protein with the correct amino acid sequence to be expressed in a non-human cell line. However, non-human cells lack the appropriate cellular machinery, such as specific glycosyltransferases, necessary to produce the correct human post-translational modifications of a protein. An extreme example is seen in E. coli cells, which produce recombinant proteins with no glycosylation, as the above figure illustrates. Rodent and yeast cells are able to glycosylate proteins, but they are still different from glycosylation in human cells. Expression System Resultant Proteins Human (e.g. K562, HEK293) Correct amino acid sequence Human post-translational modifications Rodent (e.g. CHO, NSO) Correct amino acid sequence Some natural glycosylation - not human-like Yeast (e.g. Pichia) Correct amino acid sequence Some natural glycosylation - not human-like E.Coli Correct amino acid sequence No PTMs Although there have been significant attempts to make non-human cell derived cytokines more human-like, there is a growing awareness that in many instances, particularly in therapeutics, cytokines should mimic those found in the body as closely as possible.

General readings

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Shu, Q., et al. Hum. Immunol. 71(10):988-991(2010)
Paradowska-Gorycka, A., et al. Scand. J. Immunol. 72(2):134-141(2010)
Tanasescu, C., et al. Eur. J. Intern. Med. 21(3):202-207(2010)
Davila, S., et al. Genes Immun. 11(3):232-238(2010)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Interleukin-17F / IL17F antibody

WB using anti-Human IL17-F antibody shows detection of a band ~15 kDa in size corresponding to recombinant human IL17-F (lane 1). Molecular weight markers are also shown (M). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution. Detection occurred using DyLight 649 conjugated anti-Rabbit IgG (p/n 611-143-122) secondary antibody diluted 1:20,000 in blocking buffer (p/n MB-070). Rabbit IgG Purified Hu E, NEUT, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-17F / IL17F antibody

Western Blot: IL17F Antibody (4H1629) [TA337155] - WB analysis on full-length recombinant IL-17F proteins of (1) Human, (2) Mouse and (3) Rat using IL17F antibody (clone 4H1629) at a concentration of 0.5 ug/ml. Note: This antibody did not detect the mouse Mouse 4H1629 Purified Hu C, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-17F / IL17F antibody

Western Blot of Peroxidase conjugated Rabbit anti-IL-17F Antibody. Lane 1: Human IL-17F. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Peroxidase IL-17F secondary antibody at 1:1,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 16kDa for Human IL-17F. Other band(s): none. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-17F / IL17F antibody

WB using anti-Mouse IL-17F antibody shows detection of a band ~18 kDa corresponding to recombinant mouse IL-17F.  Primary antibody was used at 1:1,000 dilution. Rabbit IgG Purified Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-17F / IL17F antibody

Western Blot of Mouse Anti-IL-17F antibody Lane 1: human full length recombinant IL-17F protein Lane 2: mouse full length recombinant IL-17F protein Lane 3: rat full length recombinant IL-17F protein Load: 20 ng/lane Primary antibody: Anti-IL-17F antibody at 0.2ug/mL for overnight at 4°C Mouse 4H450.1 Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-17F / IL17F antibody

Western Blot of Mouse Anti-IL-17F antibody Lane 1: human full length recombinant IL-17F protein Lane 2: mouse full length recombinant IL-17F protein Lane 3: rat full length recombinant IL-17F protein Load: 20 ng/lane Primary antibody: Anti-IL-17F antibody at 0.2ug/mL for overnight at 4°C Mouse 4H450.1 Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-17F / IL17F antibody

Western Blot of Mouse Anti-IL-17F antibody Lane 1: human full length recombinant IL-17F protein Lane 2: mouse full length recombinant IL-17F protein Lane 3: rat full length recombinant IL-17F protein Load: 20 ng/lane Primary antibody: Anti-IL-17F antibody at 0.5ug/mL for overnight at 4°C Mouse 4H1629.1 Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-17F / IL17F antibody

Western Blot of Mouse Anti-IL-17F antibody Lane 1: human full length recombinant IL-17F protein Lane 2: mouse full length recombinant IL-17F protein Lane 3: rat full length recombinant IL-17F protein Load: 20 ng/lane Primary antibody: Anti-IL-17F antibody at 0.5ug/mL for overnight at 4°C Mouse 4H1629.1 Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-17F / IL17F antibody

IL-17 producing cells were generated by culturing human PBMC with anti-Human CD3 and anti-Human CD28 or PMA and Ionomycin in the presence of Brefeldin A (2) for 16 hours.  Cell lysates were run under reducing conditions, immunoblotted with 2 ug/ml of Anti-Human IL-17F Purified and revealed with Anti-Mouse HRP. Mouse H17F10A7 Purified Hu WB
25 µg / €150.00
  OriGene Technologies, Inc.

Interleukin-17F / IL17F antibody

To detect hIL-17F by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-17F. Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Interleukin-17F / IL17F antibody

To detect hIL-17F by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-17F (TA328242) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-17F. Rabbit IgG Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-17F / IL17F antibody

Interleukin-17F / IL17F Mouse IgG2b B-F60 Azide Free Hu FN
0.5 mg / €460.00
  Acris Antibodies GmbH

Interleukin-17F / IL17F antibody

Interleukin-17F / IL17F Mouse IgG2b B-G46 Azide Free Hu E(capture), Elispot
1 mg / €790.00
  Acris Antibodies GmbH

Interleukin-17F / IL17F antibody

Interleukin-17F / IL17F Mouse IgG2b B-F60 Biotin Hu E(detection), Elispot
0.1 mg / €460.00
  Acris Antibodies GmbH

Interleukin-17F / IL17F antibody

The graph on the right represents activated TH17 cells (5h by PMA-ionomycin) marked with B-C52 and the graph on the left shows B-C52 with non-activated TH17 cells. In both cases, the dotted line represents the IgG1 Isotype Control (B-Z1) Mouse IgG1 B-C52 Azide Free Hu F
0.5 mg / €460.00
  Acris Antibodies GmbH

Interleukin-17F / IL17F antibody

The graph on the right represents activated TH17 cells (5h by PMA-ionomycin) marked with B-C52 and the graph on the left shows B-C52 with non-activated TH17 cells. In both cases, the dotted line represents the IgG1 Isotype Control (B-Z1) Mouse IgG1 B-C52 Purified Hu F
2 ml/200Tests / €250.00
  Acris Antibodies GmbH

Interleukin-17F / IL17F (N-term) antibody

Western blot analysis of IL17F (arrow) using Interleukin-17F / IL17F Antibody (N-term) Cat.-No AP52188PU-N. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the IL17F gene (Lane 2) . Rabbit Ig Aff - Purified Hu F, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Interleukin-17F / IL17F antibody

Interleukin-17F / IL17F Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

Interleukin-17F / IL17F antibody

Immunoperoxidase of purified MaxPab antibody to IL17F on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml] Mouse Purified Hu E(detection), P, WB
50 µg / €370.00
  Abnova Taiwan Corp.

Interleukin-17F / IL17F antibody

IL17F MaxPab rabbit polyclonal antibody. Western Blot analysis of IL17F expression in Jurkat. Rabbit Serum Hu E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

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