Interleukin-2 / IL2 antibody

Principal name

Interleukin-2 / IL2 antibody

Alternative names for Interleukin-2 / IL2 antibody

IL-2, TCGF, T-cell growth factor

SwissProt ID

O77620 (Rabit), P04351 (Mouse), P05016 (Bovin), P17108 (Rat), P19114 (Sheep), P26891 (Pig), P37997 (Horse), P60568 (Human), Q07885 (Felca), Q29416 (Canfa)

Gene ID

3558 (IL2), 116562 (Il2), 16183, 100034204, 751114, 443401, 280822, 396868, 3558088 (accD)

Available reactivities

Hu (Human), Rt (Rat), Ms (Mouse), Fe (Feline), Mky (Monkey), Sh (Sheep), Av (Birds), Chk (Chicken), Mam (Mammalia), Bov (Bovine), Por (Porcine)

Available hosts

Mouse, Goat, Rabbit, Rat, Chicken, Sheep

Available applications

Enzyme Immunoassay (E), ELISA (capture) (E(capture)), ELISA (detection) (E(detection)), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Functional assay (FN), Flow Cytometry (F), Immunoprecipitation (IP), Dot blot (Dot), Neutralisation (NEUT)

Background of Interleukin-2 / IL2 antibody

The immune system reacts to a pathogen by activation of balanced network of the humoral and cellular immune responses. Subsequently the activated condition of the immune system will, after the elimination of the pathogen, be down-regulated to a balanced situation again. Control of the immune response requires efficient communication between the different cells involved in this response. This interaction is provided by cell/cell contact and by a complex array of mediators. Among these mediators cytokines, soluble factors produced by these cells, play an important role. Cytokines can act on other cells locally or distantly, but can be even auto regulating. Cytokines can behave stimulatory or inhibitory, or can even perform both activities, depending on the (pre)activation stage of the target cell. (3, 4)
Interleukine 2 (IL-2) is a cytokine expressed by human lymphocytes that have been stimulated by antigens or mitogens. Without stimulation, IL-2 is produced in low amounts by TH1 cells in vivo. Originally, IL-2 was referred to as T cell growth factor. T cell hybridomas or a leukemia cell line such as Jurkat, produce increased amounts of IL-2 in vitro. Stimulation with IL-2 leads to rapid clonal expansion of T cells and IL-2 acts on a variety of cells in vivo, such as B cells, NK cells, monocytes and macrophages. Furthermore, IL-2 can induce activation of LAK cells.

General readings

Sanchez, R., C. Williams, J.L. Daza, Q.H. Dan, Q.C. Xu, Y.J. Chen, C. Delgado, N. Arpajirakul, E.W.B. Jeffes, R.C. Kim, T. Douglass, U. Al Atar, H.T. Wepsic, and M.R. Jadus (2002) T9 glioma cells expressing membrane-macrophage colony stimulating fact or produce CD4+T cell-associated protective immunity against T9 intracranial gliomas and systemic immunity against different syngeneic gliomas. Cellular Immunology 215(1):1 - 11.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Interleukin-2 / IL2 antibody

Western blot analysis of CTLL-2 cell lysate using IL-2 antibody. Rabbit IgG Purified Hu, Ms
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-2 / IL2 antibody

Western blot analysis using recombinant human IL-2. Lane 1: 50 ng, Lane 2:100 ng. Rabbit Purified Ms NEUT, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-2 / IL2 antibody

Western blot analysis of IL-2 using recombinant rat IL-2. Rabbit Purified Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-2 / IL2 antibody

anti-Human IL-2 antibody shows detection of a band ~15 kDa in size corresponding to recombinant human IL-2. The identity of the faint higher molecular weight band may represent a homodimer. Molecular weight markers are also shown (left). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution. Detection occurred using peroxidase conjugated anti-Rabbit IgG (p/n 611-103-122) secondary antibody diluted 1:40,000. Rabbit IgG Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-2 / IL2 antibody

Western Blot of Rabbit anti-IL-2 Antibody Peroxidase Conjugated Lane 1: Human IL-2 Recombinant Protein Load: 50 ng per lane Secondary antibody: Peroxidase Conjugated IL-2 Antibody at 1:1000 for 60 min at RT Block: MB-070 for 30 min at RT Predicted/Observed size: 16 kDa, 16 kDa Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-2 / IL2 antibody

To detect hIL-2 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-2. Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Interleukin-2 / IL2 antibody

To detect hIL-2 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-2. Goat IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Interleukin-2 / IL2 antibody

To detect hIL-2 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-2 (TA328205) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-2. Goat IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-2 / IL2 antibody

To detect hIL-2 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-2 (TA328204) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-2. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-2 / IL2 antibody

To detect mIL-2 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant mIL-2. Rabbit IgG Purified E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Interleukin-2 / IL2 antibody

To detect mIL-2 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine IL-2 (TA328480) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-2. Rabbit IgG Purified E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-2 / IL2 antibody

To detect Rat IL-2 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat IL-2. Rabbit IgG Purified Rt E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Interleukin-2 / IL2 antibody

To detect Rat IL-2 by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Rat IL-2 (TA328566) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-2. Rabbit IgG Purified Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-2 / IL2 antibody

Western blot analysis of Interleukin 2 expression in HT-29 cells Rabbit IgG Purified Hu, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-2 / IL2 antibody

Interleukin-2 / IL2 Mouse IgG1 B025M Azide Free Hu E, FN
0.2 mg / €760.00
  Acris Antibodies GmbH

Interleukin-2 / IL2 antibody

Interleukin-2 / IL2 Rat IgG I2-C7 Azide Free Ms FN
0.2 mg / €340.00
  Acris Antibodies GmbH

Interleukin-2 / IL2 antibody

Interleukin-2 / IL2 Mouse IgG1 419A-7A3 Purified Hu C, E, F, WB
0.5 mg / €680.00
  Acris Antibodies GmbH

Interleukin-2 / IL2 antibody

Interleukin-2 / IL2 Mouse IgG2b 297C16G2 Purified Hu C, E
0.5 mg / €680.00
  Acris Antibodies GmbH

Interleukin-2 / IL2 antibody

Interleukin-2 / IL2 Mouse IgG2a 297C16F11 Azide Free Hu C, Dot, FN
0.5 mg / €680.00
  Acris Antibodies GmbH

Interleukin-2 / IL2 antibody

Interleukin-2 / IL2 Mouse 12B11AE7 Biotin Rt E
0.1 mg / €550.00
  Acris Antibodies GmbH

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