Interleukin-21 / IL21 antibody

Principal name

Interleukin-21 / IL21 antibody

Alternative names for Interleukin-21 / IL21 antibody

IL-21, Za11

SwissProt ID

A3QPB9 (Rat), Q6L7I9 (Canfa), Q76LU5 (Bovin), Q76LU6 (Pig), Q9ES17 (Mouse), Q9HBE4 (Human)

Gene ID

59067 (IL21), 378475, 60505 (Il21), 365769

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Eq (Equine), Gt (Goat), Por (Porcine), Sh (Sheep)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of Interleukin-21 / IL21 antibody

Interleukin-21 (IL-21) is a secreted, type-I cytokine with immunoregulatory activity. Human IL-21 shares the common ?-chain with IL-2, IL-4, IL-7, IL-9, and IL-15 proteins but, in addition, binds to a unique IL-21Ra chain which triggers a cascade of events which includes activation of the tyrosine kinases JAK1 and JAK3, followed by activation of the transcription factors STAT1 and STAT3. Bovine and human IL-21 have pleiotropic functions and are mainly produced by activated T-cells in response to antigenic stimulation, but target a broad range of lymphoid and myeloid cells of the immune system (T cells, B cells, natural killer (NK) cells and dendritic cells). IL21 is therefore is able to regulate innate and acquired immune responses. The biological effects of IL-21 include induction of differentiation, maturation, and proliferation of T-cells-stimulated B-cells into plasma cells and memory B-cells, stimulation (in conjunction) with IL-4 of IgG production, and induction of apoptotic effects in naive B-cells and stimulated B-cells in the absence of T-cell signaling. Human IL-21 has also been shown to promote the anti-tumor activity of CD8+ T-cells and NK cells. During T-cell mediated immune response, IL21 may inhibit dendritic cells’ (DC) activation and maturation. In synergy with IL15 and IL18, IL21 stimulates interferon gamma production in T-cells and NK cells; with the IL15, it may play a role in proliferation and maturation of natural killer (NK) cells.
The open reading frame of the bovine IL-21 cDNA is 459 bp in length and encodes 152 amino acids. The predicted amino acid sequence is 78-81% and 58-67% homologous to the predicted human and murine IL-21 amino acid sequences, respectively. In one study, recombinant bovine IL-21 strongly induced NK cell proliferation using a human NK cell-line, NK0, and enhanced the lymphokine activated killer (LAK) activity of bovine peripheral blood mononuclear cells. In another by the same authors, recombinant bovine mature IL-21 induced the proliferation of human IL-2-dependent cells, ILT-MAT

General readings

Parrish-Novak, J. et al. (2000) Nature 408: 57.

Parrish-Novak, J. et al. (2002) J Leukoc Biol 72: 856.

Konforte, D. et al. (2009) J Immunol 182: 1781.

Strengell, M. et al. (2002) J Immunol 169: 3600.

Davis, I.D. et al. (2007) Clin Cancer Res 13: 6926.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Interleukin-21 / IL21 antibody

  Rabbit Polyclonal IL-21 Antibody  
Western blot analysis of IL-21 expression in HL-60 cell lysate in the absence (lane A) or presence of blocking peptide (lane B) with IL-21 antibody at 1 ug /ml. Rabbit IgG Aff - Purified Hu E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-21 / IL21 antibody

  Rabbit polyclonal anti-IL21 antibody  
Western blot analysis of extracts from HuvEc cells, K562 cells, HepG2 cells and Jurkat cells, using IL21 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Interleukin-21 / IL21 antibody

  IL-21 Rabbit Polyclonal (Internal) Antibody  
Anti-IL21 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. Rabbit Aff - Purified Hu P, WB
50 µg / €400.00
  OriGene Technologies, Inc.

Interleukin-21 / IL21 antibody

  Biotinylated Anti-Human IL-21 Rabbit Polyclonal Antibody  
To detect hIL-21 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-21. Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Interleukin-21 / IL21 antibody

  Anti-Human IL-21 Rabbit Polyclonal Antibody  
To detect hIL-21 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-21 (TA328245) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-21. Rabbit IgG Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-21 / IL21 antibody

  Biotinylated Anti-Murine IL-21 Rabbit Polyclonal Antibody  
To detect mIL-21 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant mIL-21. Rabbit IgG Purified E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Interleukin-21 / IL21 antibody

  Anti-Murine IL-21 Rabbit Polyclonal Antibody  
To detect mIL-21 by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine IL-21 (TA328504) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-21. Rabbit IgG Purified E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-21 / IL21 antibody

  Mouse Polyclonal IL-21 Antibody  
Western Blot: IL21 Antibody [TA337200] - Analysis using Full-length recombinant IL-21 protein probed with 1 ug/ml of . Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used. Mouse Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-21 / IL21 antibody

  Rabbit Polyclonal Anti-IL21 Antibody  
Host: Rabbit; Target Name: IL21; Sample Tissue: Fetal Liver lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Can, Eq, Gt, Hu, Por, Sh WB
50 µg / €325.00
  OriGene Technologies, Inc.

Interleukin-21 / IL21 antibody

Interleukin-21 / IL21 Mouse IgG1 B-P41 Azide Free Hu E
0.5 mg / €460.00
  Acris Antibodies GmbH

Interleukin-21 / IL21 antibody

Interleukin-21 / IL21 Mouse IgG1 B-Z16 Azide Free Hu E
0.5 mg / €460.00
  Acris Antibodies GmbH

Interleukin-21 / IL21 antibody

Western blot using anti-bovine IL-21 antibody shows detection of recombinant bovine IL-21 at 15.1kDa (arrow) raised in yeast. Protein was purified and resolved by SDS-PAGE, transferred to PVDF membrane. Membrane was blocked with 3% BSA (BSA-30, diluted 1:10), and probed with Rockland Immunochemicals, Inc. Anti-bovine IL-21. After washing, membrane was probed with Dylight(TM) 649 Conjugated Anti-Rabbit IgG (H&L) (Donkey) Antibody Rabbit IgG Aff - Purified Bov E, WB
0.1 mg / €450.00
  Acris Antibodies GmbH

Interleukin-21 / IL21 antibody

Western blot using anti-bovine IL-21 antibody shows detection of recombinant bovine IL-21 at 15.1kDa (arrow) raised in yeast. Protein was purified and resolved by SDS-PAGE, transferred to PVDF membrane. Membrane was blocked with 3% BSA (BSA-30, diluted 1:10), and probed with Rockland Immunochemicals, Inc. Anti-bovine IL-21. After washing, membrane was probed with Dylight(TM) 649 Conjugated Anti-Rabbit IgG (H&L) (Donkey) Antibody Rabbit IgG Aff - Purified Bov E, WB
25 µl / €190.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

IL21 - human gene knockout kit via CRISPR

IL21 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

IL21 - human gene knockout kit via CRISPR

IL21 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

IL21 - human gene knockout kit via CRISPR

IL21 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

IL21 - human gene knockout kit via CRISPR

IL21 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Il21 - mouse gene knockout kit via CRISPR

Il21 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Il21 - mouse gene knockout kit via CRISPR

Il21 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Il21 - mouse gene knockout kit via CRISPR

Il21 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

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