Interleukin-23 alpha / IL23A antibody

Principal name

Interleukin-23 alpha / IL23A antibody

Alternative names for Interleukin-23 alpha / IL23A antibody

IL-23 subunit alpha, SGRF, Interleukin-23 subunit p19, IL-23p19

SwissProt ID

Q64FU1 (Horse), Q6LA37 (Cavpo), Q91Z84 (Rat), Q9EQ14 (Mouse), Q9N2H9 (Pig), Q9NPF7 (Human)

Gene ID

51561 (IL23A), 83430 (Il23a)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit, Rat

Available applications

ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Frozen Sections (C), Immunoprecipitation (IP), Enzyme Immunoassay (E), Functional assay (FN), ELISA (capture) (E(capture)), Flow Cytometry (F)

Background of Interleukin-23 alpha / IL23A antibody

The 473P19 antibody reacts with the p19 subunit of human IL-23. The 473P19 antibody was generated from immunization with authentic, insect cell-expressed, recombinant human IL-23 heterodimer. The use of a p19-specific capture antibody and a p40-specific detection antibody yields a human IL-23 sandwich ELISA exquisitely specific for human IL-23. IL-12 p40 monomer and IL-12 p70 were run in the assay at 200 ng/ml with no interference or cross-reactivity observed. A panel of 20 unrelated cytokines was also run in the IL-23 ELISA at 100 ng/ml with no cross reactivity observed. The assay has been validated by specific detection of significant levels of native human IL-23 protein in supernatants from a variety of different activated dendritic cell populations. IL-23 is a heterodimeric cytokine composed of the p40 subunit of IL-12 disulfide-linked with a protein p19. p19, like p35 of IL-12, is biologically inactive by itself. IL-23 interacts with IL-12Rbeta1 and an additional, novel beta2-like receptor subunit with STAT4 binding domain, termed IL-23R. IL-23 is secreted by activated mouse and human dendritic cells. Biological activities of mouse IL-23 are distinct from those of mouse IL-12. Mouse IL-23 was found not to induce significant amounts of IFN-g. Mouse IL-23 does induce strong proliferation of memory T cells (but not nave T cells), whereas IL-12 has no effect on memory cells. Additionally, mouse IL-23 (but not IL-12) can activate mouse memory T cells to produce the proinflammatory cytokine IL-17. Human IL-23 has biological properties which are less distinct from human IL-12 human IL-23 induces proliferation of memory T cells and induces moderate levels of IFN-g production by nave and memory T cells, as compared to IL-12. IL-23-dependent, IL-17-producing CD4+ T cells (Th-17 cells) have been identified as a unique subset of Th cells that develops along a pathway that is distinct from the Th1- and Th2- cell differentiation pathways. The hallmark effector molecules of Th1 and Th2 cells, e.g., IFN-g and IL-4, have each been found to negatively regulate the generation of these Th-17 cells. More recently, de novo differentiation of Th-17 cells in the absence of IL-23 has been demonstrated by treatment of nave CD4 cells with TGF-beta1 and IL-6.

General readings

1. "Novel p19 protein engages IL-12p40 to form a cytokine, IL-23, with biological activities similar as well as distinct from IL-12."
Oppmann B. and Coll., Immunity 13:715-725(2000).
2. "SGRF; a novel member of the IL-6/G-CSF family." Hirata Y., Kosuge Y. Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases.
3. "The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F. and coll., Genome Res. 13:2265-2270(2003).
4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Interleukin-23 alpha / IL23A antibody

Anti-IL-23 p19 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
50 µg / €345.00
  OriGene Technologies, Inc.

Interleukin-23 alpha / IL23A (C-term) antibody

Western blot analysis of IL-23 in Raji lysate with IL-23 antibody at 1 ug/mL in the (A) absence and (B) presence of blocking peptide. Rabbit IgG Aff - Purified Hu, Ms E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-23 alpha / IL23A (N-term) antibody

Western blot analysis of IL-23 in mouse pancreas tissue lysate with IL-23 antibody at (A) 1 and (B) 2 ug/ml. Rabbit IgG Aff - Purified Hu, Ms E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-23 alpha / IL23A antibody

Anti-IL-23 p19 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. Rabbit Aff - Purified Hu, Ms P, WB
50 µg / €345.00
  OriGene Technologies, Inc.

Interleukin-23 alpha / IL23A antibody

AM20386PU-N IL-23 staining of Formalin-Fixed, Paraffin-Embedded Human Lung, vasculature Mouse IgG1 HLT2736 Purified Hu C, IP, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Interleukin-23 alpha / IL23A antibody

Labelling on Paraffin Section from psoriasis lesions with IL23 Antibody Clone B-Z23 diluted at 1/500 (background colouration by DAPI). Mouse IgG1 B-Z23 Azide Free Hu FN, P
0.5 mg / €460.00
  Acris Antibodies GmbH

Interleukin-23 alpha / IL23A antibody

Interleukin-23 alpha / IL23A Mouse IgG1 B-B23 Azide Free Ms
0.5 mg / €460.00
  Acris Antibodies GmbH

Interleukin-23 alpha / IL23A antibody

Interleukin-23 alpha / IL23A Mouse IgG1 B-Z23 Azide Free Hu E(capture), Elispot
1 mg / €790.00
  Acris Antibodies GmbH

Interleukin-23 alpha / IL23A antibody

Interleukin-23 alpha / IL23A Mouse IgG1 B-F43 Biotin Hu E(detection)
0.1 mg / €460.00
  Acris Antibodies GmbH

Interleukin-23 alpha / IL23A (C-term) antibody

Immunocytochemical staining of Raji cells with Rabbit anti Human interleukin-23 (AP05588PU-N) Rabbit IgG Purified Hu, Ms C, ICC/IF, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

Interleukin-23 alpha / IL23A (N-term) antibody

Immunohistochemical staining of mouse pancreas With Rabbit anti Human interleukin-23 (AP05589PU-N) Rabbit IgG Purified Hu, Ms WB
0.1 mg / €440.00
  Acris Antibodies GmbH

Interleukin-23 alpha / IL23A Control Peptide

Interleukin-23 alpha / IL23A
50 µg / €160.00
  Acris Antibodies GmbH

Interleukin-23 alpha / IL23A Control Peptide

Interleukin-23 alpha / IL23A
50 µg / €160.00
  Acris Antibodies GmbH

Interleukin-23 alpha / IL23A antibody

Western Blot analysis of IL23A expression in transfected 293T cell line (H00051561-T01) by IL23A MaxPab polyclonal antibody.

Lane 1: IL23A transfected lysate(20.79 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

Interleukin-23 alpha / IL23A antibody

Western Blot analysis of IL23A expression in transfected 293T cell line (H00051561-T01) by IL23A MaxPab polyclonal antibody.

Lane 1: IL23A transfected lysate(20.79 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

Interleukin-23 alpha / IL23A antibody

Immunoprecipitation of IL23A transfected lysate using anti-IL23A MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with IL23A MaxPab mouse polyclonal antibody (B01) (H00051561-B01). Rabbit Serum Hu E(detection), IP
0.1 ml / €380.00
  Abnova Taiwan Corp.

Interleukin-23 alpha / IL23A antibody

Western Blot analysis of IL23A expression in transfected 293T cell line (H00051561-T01) by IL23A MaxPab polyclonal antibody.

Lane 1: IL23A transfected lysate(20.70 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

Interleukin-23 alpha / IL23A antibody

Interleukin-23 alpha / IL23A Mouse IgG2a 4C8 Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Interleukin-23 alpha / IL23A antibody

Immunocytochemistry of IL23A in Raji cells with IL23A polyclonal antibody ( Cat # PAB13197 ) at 10 µg/ml. Rabbit Hu, Ms ICC/IF, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

Interleukin-23 alpha / IL23A antibody

Immunohistochemistry of IL23A in mouse pancreas tissue with IL23A polyclonal antibody ( Cat # PAB13198 ) at 2 µg/ml. Rabbit Hu, Ms P, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

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