Interleukin-27 / IL27A antibody

Principal name

Interleukin-27 / IL27A antibody

Alternative names for Interleukin-27 / IL27A antibody

IL-27 subunit alpha, IL27-A, p28, IL30, IL-30, Interleukin-30

SwissProt ID

Q5S1V9 (Pig), Q8K3I6 (Mouse), Q8NEV9 (Human)

Gene ID

246778 (IL27), 246779 (Il27)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit, Rat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Immunoprecipitation (IP), Flow Cytometry (F), ELISA (capture) (E(capture)), Frozen Sections (C)

Background of Interleukin-27 / IL27A antibody

The cytokine Interleukin 27 (IL-27) is produced in response to inflammation. It is made by activated antigen presenting cells including monocytes, endothelial cells, and dendritic cells. IL-27 consists of a heterodimeric combination of Epstein-Barr virus-induced molecule 3 (EBI3, or IL-27B) non-covalently linked with IL-27 p28 (or IL-27A). It is a regulator of T helper cell development and suppressor of T-cell proliferation. IL-27 has both pro- and anti-inflammatory properties. It can stimulate cytotoxic T cell activity and induce isotype switching in B-cells. It has diverse effects on innate immune cells. It induces monocytes and mast cells to secrete pro-inflammatory cytokines. When infection is present, IL-27 induces naive CD4+ T cells to proliferate and develop Th1 cell responses. As an anti-inflammatory regulator, IL-27 can inhibit Th1 or Th2 responses and restrict the strength and duration of adaptive immune responses.

The IL-27 p28 subunit, a 28 kDa glycoprotein belonging to the type I cytokine family, is homologous to IL-12 p35, IL-23 p19, and IL-6. The EBI3 (Epstein-Barr virus-induced molecule 3, or IL-27B) subunit is a 34 kDa glycoprotein containing two fibronectin type III domains, and belongs to the type I cytokine receptor family. It can exist as a homodimer and can also heterodimerize with IL-12 p35. It is homologous to the p40 subunit of IL-12 and IL-23 and to the extracellular domain of IL-6 R. EBI3 can heterodimerize also with IL-12 p35, or can exist as a homodimer.

The heterodimeric IL-27 receptor contains WSX-1 (TCCR) and gp130. WSX-1 is specific for IL-27, and is expressed on resting/naive CD4+ T cells, CD8+ T cells, NK cells, dendritic cells, monocytes, mast cells, and B cells. Gp130, on the other hand, functions as a subunit of the receptor complexes for at least seven other cytokines. IL-27 also promotes effector functions of NK cells and CD8+ T cells.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Interleukin-27 / IL27A (N-term) antibody

Western blot analysis of IL-27 in EL4 lysate with IL-27 antibody at (A) 2 and (B) 4 ug/mL. Rabbit IgG Aff - Purified Hu, Ms E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-27 / IL27A antibody

Western blot analysis of IL-27 in Daudi lysate with IL-27 antibody at (A) 2 and (B) 4 µg/ml. Rabbit IgG Aff - Purified Hu, Ms E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-27 / IL27A antibody

Detection of recombinant IL27/p28 protein by anti-Mouse IL-27/p28 antibody. Recombinant mouse IL27/p28 was loaded on to an SDS-PAGE gel at 0.25 µg and after separation, transferred to nitrocellulose. The membrane was incubated with primary antibody diluted 1:1,000 in 1% BSA in TBST overnight at 4°C. After washes, the blot was reacted with secondary antibody HRP Goat anti-Rabbit IgG antibody diluted 1:40,000 in blocking buffer (p/n MB-070) for 30 min at RT. Rabbit IgG Ig Fraction Ms E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-27 / IL27A antibody

Recombinant mouse IL27/p28 was loaded at 0.25 µg. The blot was blocked with 1% BSA in TBST for 30 min at RT. Blot was incubated with HRP rabbit anti-Mouse IL-27/p28 in 1% BSA/TBST at 1:5,000 for 30 min at RT. Data was collected using Bio-Rad VersaDoc® 4000 MP. Rabbit IgG HRP Ms E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-27 / IL27A antibody

Anti-IL-27/p28 antibody in WB shows detection of recombinant mouse IL-27/p28. Recombinant protein (0.1 µg) was loaded on to an SDS-PAGE gel, and after separation, transferred to nitrocellulose. The expected band is approximately 26 kDa in size. The membrane was incubated with anti-IL-27/p28 antibody diluted 1:1,000 in 1% BSA in TBST overnight at 4°C. After washes, the blot was reacted with secondary antibody HRP Goat anti-Rabbit IgG antibody diluted 1:40,000. Rat 3H12.F10 Purified Ms F, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-27 / IL27A antibody

Predicted cell location: Cytoplasm . Positive control: Human lymphoma and liver cancer tissue . Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human lymphoma tissue using IL27 antibody at dilution 1/10, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Interleukin-27 / IL27A antibody

Predicted cell location: Cytoplasm . Positive control: Human cervical and liver cancer tissue . Recommended dilution: 1/15-50  The image on the left is immunohistochemistry of paraffin-embedded human cervical cancer tissue using IL27 antibody at dilution 1/15, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Interleukin-27 / IL27A antibody

Western blot analysis of extracts of various cell lines, using IL27 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-27 / IL27A antibody

WB Suggested Anti-IL27 Antibody; Titration: 1.0 ug/ml; Positive Control: Fetal Lung Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

Interleukin-27 / IL27A antibody

The graph on the left represents activated dendritic cells marked with B-G49 and the graph on the right shows B-G49 with non-activated dendritic cells. In the two cases, the dotted line represents the IgG2a isotype control (B-Z2). Mouse IgG2a B-G49 Azide Free Hu F
0.5 mg / €460.00
  Acris Antibodies GmbH

Interleukin-27 / IL27A antibody

The dendritic cells were activated with LPS 1µg/ml, IFN-gamma 10ng/ml and TNF-alpha 100ng/ml during 48h. A part of the dendritic cells was not activated to be used as Negative Control. 3x10e5 dendritic cells (activated=green line or resting cells=black line) were labelled with 10 µl of anti-IL27 antibody FITC conjugated. Mouse IgG2a B-G49 FITC Hu F
1ml (100T) / €270.00
  Acris Antibodies GmbH

Interleukin-27 / IL27A antibody

Interleukin-27 / IL27A Mouse IgG2a B-G49 Purified Hu F
2ml (200T) / €250.00
  Acris Antibodies GmbH

Interleukin-27 / IL27A antibody

The dendritic cells were activated with LPS 1µg/ml, IFN-gamma 10ng/ml and TNF-alpha 100ng/ml during 48h. A part of the dendritic cells was not activated to be used as negative control. 3x10e5 dendritic cells (activated=green line or resting cells=black line) were labelled with 10µL of anti-IL27 antibody PE conjugated. Mouse IgG2a B-G49 PE Hu F
1ml (100T) / €290.00
  Acris Antibodies GmbH

Interleukin-27 / IL27A antibody

Interleukin-27 / IL27A Mouse IgG1 B-G44 Azide Free Hu E(capture), Elispot
1 mg / €790.00
  Acris Antibodies GmbH

Interleukin-27 / IL27A antibody

Interleukin-27 / IL27A Mouse IgG2a B-G49 Biotin Hu E(detection)
0.1 mg / €460.00
  Acris Antibodies GmbH

Interleukin-27 / IL27A antibody

Interleukin-27 / IL27A Mouse IgG1 B-G44 Azide Free Hu E
0.5 mg / €460.00
  Acris Antibodies GmbH

Interleukin-27 / IL27A (N-term) antibody

Formalin-Fixed, Paraffin-Embedded  Human Lung stained with Interleukin-27 / IL27A Antibody Cat.-No AP08877PU-N at 5 µg/ml after heat-induced antigen retrieval. Rabbit IgG Aff - Purified Hu, Ms ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Interleukin-27 / IL27A (center) antibody

Spleen, neutrophils: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Interleukin-27 / IL27A Control Peptide

Interleukin-27 / IL27A
50 µg / €160.00
  Acris Antibodies GmbH

Interleukin-27 / IL27A Control Peptide

Interleukin-27 / IL27A
50 µg / €160.00
  Acris Antibodies GmbH

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