Interleukin-3 / IL3 antibody

Principal name

Interleukin-3 / IL3 antibody

Alternative names for Interleukin-3 / IL3 antibody

IL-3, Multipotential colony-stimulating factor, Hematopoietic growth factor, P-cell-stimulating factor, MCGF, Mast cell growth factor

SwissProt ID

P01586 (Mouse), P04823 (Rat), P08700 (Human), P49875 (Bovin), Q06435 (Sheep), Q9BDX4 (Canfa)

Gene ID

3562 (IL3), 16187 (Il3), 24495

Available reactivities

Hu (Human), Ms (Mouse), Eq (Equine), Rt (Rat)

Available hosts

Mouse, Rabbit, Rat, Sheep

Available applications

Enzyme Immunoassay (E), ELISA (capture) (E(capture)), ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Functional assay (FN), Neutralisation (NEUT), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Paraffin Sections (P), Immunoprecipitation (IP), Frozen Sections (C)

Background of Interleukin-3 / IL3 antibody

Interleukin-3 (IL-3) is a pleiotropic cytokine that regulates the proliferation, maturation, and survival of progenitor cells of the myeloid, erythroid, and megakaryocyte lineage. IL-3 is predominantly produced by activated T cells although expression has also been detected in monocytes and macrophages, NK-cells, mast cells, endothelial cells, and keratinocytes. IL-3 also may promote dendritic cell formation from CD34 progenitors in the presence of TNF-alpha, induce phagocytosis in stimulated macrophages, and up-regulate the expression of IL-1, IL-6, and TNF-alpha. Furthermore, IL-3 can induce the synthesis of histamines in mast cells and the expression of complement factor C3a receptors on basophils. IL-3 has also been shown to recruit eosinophils and increase platelet and neutrophil levels. IL-3 may contribute to TH2 responses by inducing IL-10 and IL-13 in mast cells and IL-4 and IL-3 in non-B non-T cells. The potential clinical roles for IL-3 relate to its ability, either alone or in combination with G-CSF or GM-CSF, to expand haemopoietic stem cell populations. Human IL-3 is a 133 amino acid protein that contains two N-linked glycosylation sites and exists as a monomer. This is a HCX protein. HCX Expression System Details HCX proteins mimic the proteins in the human body because they are expressed from human, rather than animal, insect or bacterial cells. This process gives them human post-translational modifications. Recombinant DNA techniques allow a human protein with the correct amino acid sequence to be expressed in a non-human cell line. However, non-human cells lack the appropriate cellular machinery, such as specific glycosyltransferases, necessary to produce the correct human post-translational modifications of a protein. An extreme example is seen in E. coli cells, which produce recombinant proteins with no glycosylation, as the above figure illustrates. Rodent and yeast cells are able to glycosylate proteins, but they are still different from glycosylation in human cells. Expression System Resultant Proteins Human (e.g. K562, HEK293) Correct amino acid sequence Human post-translational modifications Rodent (e.g. CHO, NSO) Correct amino acid sequence Some natural glycosylation - not human-like Yeast (e.g. Pichia) Correct amino acid sequence Some natural glycosylation - not human-like E.Coli Correct amino acid sequence No PTMs Although there have been significant attempts to make non-human cell derived cytokines more human-like, there is a growing awareness that in many instances, particularly in therapeutics, cytokines should mimic those found in the body as closely as possible.

General readings

Yokota. T., et al. (1984) Proc. Natl. Acad. Sci. USA 81:1070.Miyatake., S. et al. (1985) Proc. Natl. Acad. Sci. USA 82:316.Knepper T.P., et al. (1992) Biochemistry 31:11651-11659.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Interleukin-3 / IL3 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-IL-3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500182) Mouse IgG1 OTI3B11 Purified Hu ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

Interleukin-3 / IL3 antibody

Immunohistochemistry-Paraffin: IL3 Antibody (3B11) [NBP1-47806] - Immunohistochemical staining of paraffin-embedded Carcinoma of lung using anti-IL-3 mouse monoclonal antibody. Mouse 3B11 Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €620.00
  Novus Biologicals Inc.

Interleukin-3 / IL3 antibody

Western blot analysis using recombinant human IL-3. Lane 1: 50 ng, Lane 2: 250 ng, Lane 3: 1 ug. Rabbit Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-3 / IL3 antibody

anti-Human IL-3 antibody shows detection of a band ~15 kDa in size corresponding to recombinant human IL-3. The identity of the faint higher molecular weight band may represent a homodimer. Molecular weight markers are also shown (left). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution. Detection occurred using peroxidase conjugated anti-Rabbit IgG (p/n 611-103-122) secondary antibody diluted 1:40,000 Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-3 / IL3 antibody

Predicted band size: 17 kDa . Positive control: Mouse thymus tissue lysate. Recommended dilution: 1/200-1000. (Gel: 12%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/350 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Interleukin-3 / IL3 antibody

To detect hIL-3 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-3. Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Interleukin-3 / IL3 antibody

To detect hIL-3 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-3 (TA328208) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-3. Rabbit IgG Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-3 / IL3 antibody

To detect mIL-3 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant mIL-3. Rabbit IgG Purified E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Interleukin-3 / IL3 antibody

To detect mIL-3 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine IL-3 (TA328482) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-3. Rabbit IgG Purified E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-3 / IL3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL-3 (RC210109, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL-3. Mouse IgG1 OTI5G7H9 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Interleukin-3 / IL3 antibody

Interleukin-3 / IL3 Rat IgG1 BVD3-1F9 Purified Hu E
0.5 mg / €450.00
  Acris Antibodies GmbH

Interleukin-3 / IL3 antibody

Interleukin-3 / IL3 Rat IgG1 MP2-8F8 Low Endotoxin Ms F, ICC/IF, NEUT
0.5 mg / €410.00
  Acris Antibodies GmbH

Interleukin-3 / IL3 antibody

Interleukin-3 / IL3 Rat IgG1 MP2-8F8 Purified Ms E, E(capture), F, ICC/IF
0.5 mg / €340.00
  Acris Antibodies GmbH

Interleukin-3 / IL3 antibody

Sandwich ELISA using Interleukin-3 / IL3 Antibody Cat.-No AM32452PU-N Mouse Aff - Purified Hu E, NEUT, WB
0.5 mg / €220.00
  Acris Antibodies GmbH

Interleukin-3 / IL3 antibody

Interleukin-3 / IL3 Rat IgG2 9D19 Purified Ms NEUT, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

Interleukin-3 / IL3 antibody

Interleukin-3 / IL3 Mouse IgG1 5D7 Purified Hu E, E(capture)
0.2 mg / €450.00
  Acris Antibodies GmbH

Interleukin-3 / IL3 antibody

Interleukin-3 / IL3 Rabbit Biotin Ms E, WB
25 µg / €230.00
  Acris Antibodies GmbH

Interleukin-3 / IL3 antibody

Interleukin-3 / IL3 Rabbit Biotin Ms E, WB
50 µg / €340.00
  Acris Antibodies GmbH

Interleukin-3 / IL3 antibody

Interleukin-3 / IL3 Rabbit Aff - Purified Ms E, FN, WB
50 µg / €230.00
  Acris Antibodies GmbH

Interleukin-3 / IL3 antibody

Interleukin-3 / IL3 Rabbit Aff - Purified Ms E, FN, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

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