Interleukin-3 / IL3 antibody

Principal name

Interleukin-3 / IL3 antibody

Alternative names for Interleukin-3 / IL3 antibody

IL-3, Multipotential colony-stimulating factor, Hematopoietic growth factor, P-cell-stimulating factor, MCGF, Mast cell growth factor

SwissProt ID

P01586 (Mouse), P04823 (Rat), P08700 (Human), P49875 (Bovin), Q06435 (Sheep), Q9BDX4 (Canfa)

Gene ID

3562 (IL3), 16187 (Il3), 24495

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit, Rat, Sheep

Available applications

Enzyme Immunoassay (E), ELISA (capture) (E(capture)), ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Functional assay (FN), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Neutralisation (NEUT), Paraffin Sections (P), Immunoprecipitation (IP)

Background of Interleukin-3 / IL3 antibody

Kit Component:
- KN308282G1, Il3 gRNA vector 1 in pCas-Guide vector
- KN308282G2, Il3 gRNA vector 2 in pCas-Guide vector
- KN308282D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

Yokota. T., et al. (1984) Proc. Natl. Acad. Sci. USA 81:1070.
Miyatake., S. et al. (1985) Proc. Natl. Acad. Sci. USA 82:316.
Knepper T.P., et al. (1992) Biochemistry 31:11651-11659.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Interleukin-3 / IL3 antibody

  IL-3 mouse monoclonal antibody, clone OTI3B11 (formerly 3B11)  
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-IL-3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500182) Mouse IgG1 OTI3B11 Purified Hu ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

Interleukin-3 / IL3 antibody

  Rabbit polyclonal anti-IL-3 antibody  
Western blot analysis using recombinant human IL-3. Lane 1: 50 ng, Lane 2: 250 ng, Lane 3: 1 ug. Rabbit Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-3 / IL3 antibody

  Rabbit polyclonal anti-IL-3 antibody  
anti-Human IL-3 antibody shows detection of a band ~15 kDa in size corresponding to recombinant human IL-3. The identity of the faint higher molecular weight band may represent a homodimer. Molecular weight markers are also shown (left). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution. Detection occurred using peroxidase conjugated anti-Rabbit IgG (p/n 611-103-122) secondary antibody diluted 1:40,000 Rabbit IgG Purified Hu WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

Interleukin-3 / IL3 antibody

  Anti-IL3 Rabbit Polyclonal Antibody  
Predicted band size: 17 kDa . Positive control: Mouse thymus tissue lysate. Recommended dilution: 1/200-1000. (Gel: 12%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/350 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Interleukin-3 / IL3 antibody

  Biotinylated Anti-Human IL-3 Rabbit Polyclonal Antibody  
To detect hIL-3 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-3. Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Interleukin-3 / IL3 antibody

  Anti-Human IL-3 Rabbit Polyclonal Antibody  
To detect hIL-3 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-3 (TA328208) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-3. Rabbit IgG Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-3 / IL3 antibody

  Biotinylated Anti-Murine IL-3 Rabbit Polyclonal Antibody  
To detect mIL-3 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant mIL-3. Rabbit IgG Purified E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Interleukin-3 / IL3 antibody

  Anti-Murine IL-3 Rabbit Polyclonal Antibody  
To detect mIL-3 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine IL-3 (TA328482) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-3. Rabbit IgG Purified E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-3 / IL3 antibody

  anti-IL-3 mouse monoclonal antibody, clone OTI5G7H9 (formerly 5G7H9)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL-3 (RC210109, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL-3. Mouse IgG1 OTI5G7H9 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Interleukin-3 / IL3 antibody

Interleukin-3 / IL3 Rat IgG1 BVD3-1F9 Purified Hu E
0.5 mg / €450.00
  Acris Antibodies GmbH

Interleukin-3 / IL3 antibody

Interleukin-3 / IL3 Rat IgG1 MP2-8F8 Low Endotoxin Ms F, ICC/IF, NEUT
0.5 mg / €410.00
  Acris Antibodies GmbH

Interleukin-3 / IL3 antibody

Interleukin-3 / IL3 Rat IgG1 MP2-8F8 Purified Ms E, E(capture), F, ICC/IF
0.5 mg / €340.00
  Acris Antibodies GmbH

Interleukin-3 / IL3 antibody

Sandwich ELISA using Interleukin-3 / IL3 Antibody Cat.-No AM32452PU-N Mouse Aff - Purified Hu E, NEUT, WB
0.5 mg / €220.00
  Acris Antibodies GmbH

Interleukin-3 / IL3 antibody

Interleukin-3 / IL3 Rat IgG2 9D19 Purified Ms NEUT, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

Interleukin-3 / IL3 antibody

Interleukin-3 / IL3 Mouse IgG1 5D7 Purified Hu E, E(capture)
0.2 mg / €450.00
  Acris Antibodies GmbH

Interleukin-3 / IL3 antibody

Interleukin-3 / IL3 Rabbit Biotin Ms E, WB
25 µg / €230.00
  Acris Antibodies GmbH

Interleukin-3 / IL3 antibody

Interleukin-3 / IL3 Rabbit Biotin Ms E, WB
50 µg / €340.00
  Acris Antibodies GmbH

Interleukin-3 / IL3 antibody

Interleukin-3 / IL3 Rabbit Aff - Purified Ms E, FN, WB
50 µg / €230.00
  Acris Antibodies GmbH

Interleukin-3 / IL3 antibody

Interleukin-3 / IL3 Rabbit Aff - Purified Ms E, FN, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

Interleukin-3 / IL3 antibody

Interleukin-3 / IL3 Rabbit Biotin Hu E, WB
25 µg / €230.00
  Acris Antibodies GmbH

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