Interleukin-31 / IL31 antibody
Principal name
Interleukin-31 / IL31 antibody
Alternative names for Interleukin-31 / IL31 antibody
IL-31
Ncbi ID
NP_001014358.1, 10090, 9606, NP_083870.1, NP_001014358, NM_001014336, NP_083870, AAS82846, NM_029594
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F)
Background of Interleukin-31 / IL31 antibody
- Kit Component:
- - KN308283G1, Il31 gRNA vector 1 in pCas-Guide vector
- KN308283G2, Il31 gRNA vector 2 in pCas-Guide vector
- KN308283D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Thymus.
Immunohistochemistry of Il31 in rat skeletal muscle tissue with Il31 polyclonal antibody ( Cat # PAB13183 ) at 10 µg/ml.
Western blot analysis of Il31 in RAW264.7 cell lysate with Il31 polyclonal antibody ( Cat # PAB13183 ) at ( A ) 2.5, ( B ) 5 and ( C ) 10 µg/ml.
Western blot analysis of IL31 in human skeletal muscle tissue lysate with IL31 polyclonal antibody ( Cat # PAB13184 ) at ( A ) 2.5, ( B ) 5 and ( C ) 10 µg/ml.
Formalin-fixed and paraffin-embedded human testis tissue reacted with IL31 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of IL31 Antibody (N-term) Cat.-No AP52198PU-N in mouse spleen tissue lysates (35ug/lane). IL31 (arrow) was detected using the purified Pab.
Flow cytometric analysis of WiDr cells using IL31 Antibody (N-term) Cat.-No AP52198PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Representative Standard Curve
Representative Standard Curve
Western blot analysis of IL-31 in RAW264.7 cell lysate with IL-31 antibody at (A) 2.5, (B) 5 and (C) 10 ug/ml.
Immunohistochemistry of IL-31 in rat skeletal muscle tissue with IL-31 antibody at 10 ug/ml.
Western blot analysis of IL-31 in human skeletal muscle tissue lysate with IL-31 antibody at (A) 2.5, (B) 5 and (C) 10 µg/ml.
Immunofluorescence of IL-31 in Human Skeletal Muscle cells with IL-31 antibody at 20 ug/mL.
To detect hIL-31 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-31.
To detect hIL-31 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human IL-31 (TA328249) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-31.
To detect hIL-31 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-31 (TA328248) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-31.
To detect hIL-31 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-31 (TA328248) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-31.
20 ng of mouse IL-31 recombinant protein was resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-mouse IL-31 (clone 1C2B07) antibody. Proteins were visualized using an anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection.
20 ng of human IL-31 recombinant protein was resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-human IL-31 (clone 1D10B31) antibody. Proteins were visualized using an anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA306294 | Interleukin-31 / IL31 (N-term) antibody |
||||||
| Rabbit Polyclonal IL-31 Antibody | |||||||
 |
Rabbit | IgG | Aff - Purified | Ms | E, P, WB |
0.1 mg /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
|||||||
| TA306295 | Interleukin-31 / IL31 antibody |
||||||
| Rabbit Polyclonal IL-31 Antibody | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu, Ms | E, WB |
0.1 mg /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA328248 | Interleukin-31 / IL31 antibody |
||||||
| Biotinylated Anti-Human IL-31 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | E, WB |
50 µg /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA328249 | Interleukin-31 / IL31 antibody |
||||||
| Anti-Human IL-31 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | E, WB |
0.1 mg /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| AM31280AF-N | Interleukin-31 / IL31 antibody |
||||||
 |
Mouse | IgG1 | B-S31 | Azide Free | Hu |
0.5 mg /
€460.00
|
|
| Acris Antibodies GmbH | |||||||
| AM31281AF-N | Interleukin-31 / IL31 antibody |
||||||
|
Mouse | IgG2b | B-A51 | Azide Free | Hu |
0.5 mg /
€460.00
|
|
| Acris Antibodies GmbH | |||||||
| AP01149BT-N | Interleukin-31 / IL31 antibody |
||||||
|
Rabbit | Biotin | Hu | E, WB |
50 µg /
€340.00
|
||
| Acris Antibodies GmbH | |||||||
| AP01149BT-S | Interleukin-31 / IL31 antibody |
||||||
|
Rabbit | Biotin | Hu | E, WB |
25 µg /
€230.00
|
||
| Acris Antibodies GmbH | |||||||
| AP01149PU-N | Interleukin-31 / IL31 antibody |
||||||
|
Rabbit | Aff - Purified | Hu | E, WB |
0.1 mg /
€340.00
|
||
| Acris Antibodies GmbH | |||||||
| AP01149PU-S | Interleukin-31 / IL31 antibody |
||||||
|
Rabbit | Aff - Purified | Hu | E, WB |
50 µg /
€230.00
|
||
| Acris Antibodies GmbH | |||||||
| AP07760PU-N | Interleukin-31 / IL31 antibody |
||||||
 |
Rabbit | Purified | Hu, Ms | P, WB |
50 µg /
€470.00
|
||
| Acris Antibodies GmbH | |||||||
| AP52198PU-N | Interleukin-31 / IL31 (N-term) antibody |
||||||
 |
Rabbit | Ig | Aff - Purified | Hu, Ms | F, P, WB |
0.4 ml /
€370.00
|
|
| Acris Antibodies GmbH | |||||||
| +2 additional images |
|||||||
CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN223858 | IL31 - human gene knockout kit via CRISPR |
||
|
Human | GFP-Puro |
1 kit /
€1,200.00
|
| OriGene Technologies, Inc. | |||
| KN223858BN | IL31 - human gene knockout kit via CRISPR |
||
|
Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN223858LP | IL31 - human gene knockout kit via CRISPR |
||
|
Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN223858RB | IL31 - human gene knockout kit via CRISPR |
||
|
Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN308283 | Il31 - mouse gene knockout kit via CRISPR |
||
|
Mouse | GFP-Puro |
1 kit /
€1,200.00
|
| OriGene Technologies, Inc. | |||
| KN308283BN | Il31 - mouse gene knockout kit via CRISPR |
||
|
Mouse | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN308283LP | Il31 - mouse gene knockout kit via CRISPR |
||
|
Mouse | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN308283RB | Il31 - mouse gene knockout kit via CRISPR |
||
|
Mouse | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
