Interleukin-32 / IL32 antibody

Principal name

Interleukin-32 / IL32 antibody

Alternative names for Interleukin-32 / IL32 antibody

IL-32, NK4, TAIF, Natural killer cells protein 4, Tumor necrosis factor alpha-inducing factor

SwissProt ID

P24001 (Human)

Gene ID

9235 (IL32)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Neutralisation (NEUT), Immunoprecipitation (IP)

Background of Interleukin-32 / IL32 antibody

IL-6, and chemokines. IL32 is produced by T lymphocytes, natural killer cells, epithelial cells and blood monocytes (1). The proinflammatory properties of IL32 include induction of other proinflammatory cytokines and chemokines suh as TNFalfa, IL-1beta, IL6 and IL8 by activation of NF-kB and p38 mitogen activated protein kinase (1, 2). IL32 augment 10-foldproduction of IL1Band IL6 induced by muramyl dipeptides by NOD1 and NOD2 doamins through caspase 1-dependent mechanism. Elevated levels of IL32 were noted in synovial tissues of patients with rheumatoid arthritis and the levels coincide with the severity of disease (3).
The IL32 gene is located on chromosome 16p13.3 and at least 4 different mRNA transcripts resulting from mRNA splicing are known, the IL32 being the most abundant in A549 cells. IL32 binds to myeloblastin also known as Wegener autoantigen or proteinsase 3 (PR3) which is a serine protease in neutrophiles and monocytes that is capable of processing multiple biological substrate (4). There are 4 isoforms of this interleukin A, B, C and D that have an effect on induction of TNFalfa, IL-1beta, , IL6 and chemokines respectively. The IL32 alfa isoform specifically binds with a 30kDa proteinase (PR3) in macrophages (1). The IL32 alfa is secreted from several cells upon stimulation of some inflammatory cytokines such as IL-18, IL-1beta, IFN-gamma and IL-12. The IL32 also induces production of tumor necrosis factor, macrophage inflammatory protein (MIP)-2 and IL-8 in monocytic cell lines, suggesting that this factor may be involved in the inflammatory responses. Based on these findings, NK4 was renamed IL-32. IL32 induction in activated T cells causes aoptosis and downregulaiton of IL32 rescued the cells form apoptosis in Hela cells (2). IL-32 is a cell-associated proinflammatory cytokine, which is specifically stimulated by mycobacteria through a caspase-1- and IL-18-dependent production of IFNgamma (3). Proinflammatory cytokine cascades also play important role in rheumatoid arthritis, ischemic colitis, the IL32 expression is elevated in synovial fluids of these patients (4).
IL32 alfa is the shortest of the 4 isotypes of IL32 known, it is 134 amino acids ( kDa) protein, the IL32B (beta) is 188, IL32C is 168 and IL32D is 179 amino acids long proteins. Each subtype has an N-terminal segment and four kringle domains (NK4), that interact with several other proteins including the hepatic growth factor via c-met a tyrosine receptor kinase (5). IL-32 synergized with the intracellular nuclear oligomerization domain receptors (NOD1- and NOD2-specific muropeptides of peptidoglycans for the release of IL-1beta and IL-6. In contrast, IL-32 did not influence the cytokine production induced via TLRs (6).

General readings

1. "Identification of a novel gene expressed in activated natural killer cells and T cells."
Dahl C.A., Schall R.P., He H., Cairns J.S. J. Immunol. 148:597-603(1992).
2. "Interleukin-32: a cytokine and inducer of TNFalpha." Kim S.-H., Han S.-Y., Azam T., Yoon D.-Y., Dinarello C.A. Immunity 22:131-142(2005).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Interleukin-32 / IL32 (C-term) antibody

Western blot analysis of IL-32 in human spleen cell lysate with IL-32 antibody at (A) 5 and (B) 10 ug/ml shows two isoforms of IL-32. Rabbit IgG Aff - Purified Hu, Ms E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-32 / IL32 antibody

Western blot analysis of IL-32 in Jurkat cell lysate with IL-32 antibody at (A) 2.5 and (B) 5 µg/ml. Rabbit IgG Aff - Purified Hu, Ms E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-32 / IL32 (Isoform 4 / alpha) antibody

Western blot using HRP conjugated anti-Human IL-32A antibody shows detection of a band ~19 kDa in size corresponding to recombinant human IL-32A. The identity of the higher molecular weight band is unknown. Molecular weight markers are shown (left). After transfer, the membrane was blocked with 3% BSA in TBS followed by reaction with antibody at a 1:5,000 dilution for 30 min at room temperature. Detection occurred using TMB substrate. Rabbit IgG HRP Hu E, NEUT, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-32 / IL32 antibody

WB using anti-Human IL-32A antibody shows detection of a band ~19 kDa in size corresponding to recom-binant human IL-32A (lane 1).After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution. Detection occurred using peroxidase conjugated anti-Rabbit IgG (p/n 611-103-122) secondary antibody diluted 1:40,000. Rabbit IgG Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-32 / IL32 antibody

Western blot using HRP conjugated anti-Human IL-32A antibody shows detection of a band ~19 kDa in size corresponding to recombinant human IL-32A. The identity of the higher molecular weight band is unknown. Molecular weight markers are shown (left). After transfer, the membrane was blocked with 3% BSA in TBS followed by reaction with antibody at a 1:5,000 dilution for 30 min at room temperature. Detection occurred using TMB substrate. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-32 / IL32 (1-131) antibody

ICC/IF analysis of IL32 in J urkat cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti human IL32 antibody (1: 1 00) with goat anti mouse IgG - Alexa fluor 488 conjugate (Green). Mouse IgG1 AT2F9 Purified Hu E, ICC/IF, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

Interleukin-32 / IL32 (1-131) antibody

ICC/IF analysis of IL32 in J urkat cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti human IL32 antibody (1: 1 00) with goat anti mouse IgG - Alexa fluor 488 conjugate (Green). Mouse IgG1 AT2F9 Purified Hu E, ICC/IF, WB
50 µl / €230.00
  Acris Antibodies GmbH

Interleukin-32 / IL32 antibody

Interleukin-32 / IL32 Mouse IgG2b B-B52 Azide Free Hu E
0.5 mg / €460.00
  Acris Antibodies GmbH

Interleukin-32 / IL32 antibody

Interleukin-32 / IL32 Mouse IgG1 B-K42 Azide Free Hu E
0.5 mg / €460.00
  Acris Antibodies GmbH

Interleukin-32 / IL32 antibody

Western blot analysis of whole cell lysate from Jurkat human T cell leukemia cells probed with Rabbit anti Human Interleukin-32 (AP05618PU-N) at 2.5(A) and 5(B) µg/ml Rabbit IgG Purified Hu, Ms WB
0.1 mg / €440.00
  Acris Antibodies GmbH

Interleukin-32 / IL32 antibody

Immunohistochemical staining of human spleen with Rabbit anti Human interleukin-32 (AP05619PU-N) Rabbit IgG Purified Hu, Ms C, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

Interleukin-32 / IL32 antibody

Spleen: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu, Ms P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Interleukin-32 / IL32 antibody

Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Spleen. Rabbit Purified Hu, Ms P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Interleukin-32 / IL32 Control Peptide

Interleukin-32 / IL32
0.25 mg / €420.00
  Acris Antibodies GmbH

Interleukin-32 / IL32 antibody

Western blot of NK4 antibody Cat-No- AP10113PU-N (1/500). Rabbit Aff - Purified Hu, Ms, Rt C, E, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

Interleukin-32 / IL32 antibody

Immunohistochemistry (IHC) analyzes of IL-32 antibody (Cat.-No.: AP20770PU-N) in paraffin-embedded human lung carcinoma tissue. Rabbit Aff - Purified Hu P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Interleukin-32 / IL32 Control Peptide

Interleukin-32 / IL32
50 µg / €160.00
  Acris Antibodies GmbH

Interleukin-32 / IL32 Control Peptide

Interleukin-32 / IL32
50 µg / €160.00
  Acris Antibodies GmbH

Interleukin-32 / IL32 antibody

Western Blot analysis of IL32 expression in transfected 293T cell line (H00009235-T01) by IL32 MaxPab polyclonal antibody.

Lane 1: IL32 transfected lysate(20.68 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

Interleukin-32 / IL32 antibody

Western Blot analysis of IL32 expression in transfected 293T cell line (H00009235-T01) by IL32 MaxPab polyclonal antibody.

Lane 1: IL32 transfected lysate(20.68 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

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