Interleukin-33 (IL33) antibody

Principal name

Interleukin-33 (IL33) antibody

Alternative names for Interleukin-33 (IL33) antibody

IL-33, IL1F11, IL-1F11, NFHEV, NF-HEV, C9orf26, Interleukin-1 family member 11, Nuclear factor from high endothelial venules

SwissProt ID

O95760 (Human), O97863 (Canfa), Q66H70 (Rat), Q8BVZ5 (Mouse)

Gene ID

90865 (IL33), 77125 (Il33)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Functional assay (FN), Frozen Sections (C)

Background of Interleukin-33 (IL33) antibody

IL-33 is a member of the IL-1 family of cytokines, which includes IL-1alpha, IL-1 beta, IL-18 and IL-1Ra. In humans, IL-33 mRNA is predominantly found in dermal fibroblasts, bronchial and small airway epithelial cells and smooth muscle cells of skin and lung tissues. This cytokine is localized primarily in the nucleus where it is thought to play a role in transcriptional repression but can also be released from the cell. Upon wounding or infection, IL-33 is released from the cell via an atypical secretory pathway. During apoptosis, IL-33 is cleaved by caspases-3 and 7 (and most likely not caspase-1) to produce an 18-kDa fragment which is released. This cleaved form of IL-33 can bind the ST2 receptor, without subsequent signaling events. Necrosis-mediated release of IL-33 enables binding to ST2, a member of the IL-1 receptor family found on mast and Th2 effector cells, leading to the release of Th2 cytokines. The cytokine production due to IL-33 in turn activates human basophils. IL-33 influence on activation, degranulation, enhanced adhesion and survival of eosinophils has been shown. IL-33 also increases synthesis of IL-6, IL-13, IL-1beta, TNF-alpha, prostaglandin D2 and MCP-1 by primary bone marrow-derived mast cells. Other cells activated by IL-33 are cardiomyocytes, glial cells and CD34+ cells.

General readings

1. Schmitz J, Owyang A, Oldham E, et al. IL-33, and interleukin-1-like cytokine that signals via the IL-1 receptor-related protein ST2 and induces T helper type 2-associated cytokines. Immunity 2005; 23:479-90.
2. Dinarello CA. Interleukin-18, a proinflammatory cytokine. Eur. Cytokine Netw. 2000; 11:483-6.
3. Brint EK, Xu D, Liu H, et al. ST2 is an inhibitor of interleukin 1 receptor and Toll-like receptor 4 signaling and maintains endotoxin tolerance. Nat. Immunol. 2004; 5:373-9.
4. Chackerian AA, Oldham ER, Murphy EE, et al. IL-1 receptor
accessory protein and ST2 comprise the IL-33 receptor complex. J. Immunol. 2007; 179:2551-5.
5. Carriere V, Roussel L, Ortega N, et al. IL-33, the IL-1-like cytokine ligand for ST2 receptor, is a chromatin-associated nuclear factor in vivo. Proc. Natl. Acad. Sci. USA 2007; 104:282-7.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Interleukin-33 (IL33) antibody

Western Blot unreduced using Interleukin-33 antibody Cat.-No AP32086BT Rabbit Biotin Hu E, WB
50 µg / €340.00
  Acris Antibodies GmbH

Interleukin-33 (IL33) antibody

Western Blot unreduced using Interleukin-33 antibody Cat.-No AP32086BT Rabbit Biotin Hu E, WB
25 µg / €230.00
  Acris Antibodies GmbH

Interleukin-33 (IL33) antibody

Formalin-Fixed, Paraffin-Embedded Sections of human breast malignant ductal adenocarcinoma stained with Interleukin-33 antibody Cat.-No AP32086PU Rabbit Aff - Purified Hu E, FN, P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

Interleukin-33 (IL33) antibody

Formalin-Fixed, Paraffin-Embedded Sections of human breast malignant ductal adenocarcinoma stained with Interleukin-33 antibody Cat.-No AP32086PU Rabbit Aff - Purified Hu E, FN, P, WB
50 µg / €230.00
  Acris Antibodies GmbH

Interleukin-33 (IL33) (165-178) antibody

AP32727PU-N IL33 Antibody staining of Jurkat lysate at 1µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu WB
0.1 mg / €340.00
  Acris Antibodies GmbH

Interleukin-33 (IL33) (N-term) antibody

Western blot analysis of IL-33 in human lymph node tissue lysate with IL-33 antibody at (A) 1 and (B) 2 ug/mL. Rabbit IgG Aff - Purified Hu E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-33 (IL33) (Center) antibody

Western blot analysis of IL-33 in human lymph node tissue lysate with IL-33 antibody at (A) 1 and (B) 2 µg/ml. Rabbit IgG Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-33 (IL33) antibody

Western blot using HRP conjugated anti-Human IL-33 antibody shows detection of a band ~18 kDa in size corresponding to recombinant human IL-33. The identity of the higher molecular weight band is unknown. Molecular weight markers are shown (left). After transfer, the membrane was blocked with 3% BSA in TBS followed by reaction with antibody at a 1:5,000 dilution for 30 min at room temperature. Detection occurred using TMB substrate. Rabbit IgG HRP Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-33 (IL33) antibody

WB using anti-Human IL-33 antibody shows detection of a band ~18 kDa in size corresponding to recom-binant human IL-33 (lane 1). The identity of the higher molecular weight band is unknown. Molecular weight markers are also shown (M). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution. Detection occurred using peroxidase conjugated anti-Rabbit IgG (p/n 611-103-122) secondary antibody diluted 1:40,000. Rabbit IgG Ig Fraction Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-33 (IL33) antibody

WB using anti-Human IL-33 antibody shows detection of a band ~18 kDa in size corresponding to recom-binant human IL-33 (lane 1). The identity of the higher molecular weight band is unknown. Molecular weight markers are also shown (M). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution. Detection occurred using peroxidase conjugated anti-Rabbit IgG (p/n 611-103-122) secondary antibody diluted 1:40,000. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-33 (IL33) antibody

Western blot analysis of IL-33 using 125ng of recombinant IL-33 with IL-33 antibody at 1 ug/mL. Mouse IgG 12B3C4 Aff - Purified Hu, Ms E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-33 (IL33) antibody

Western blot analysis of IL-33 using 125ng of recombinant IL-33 with IL-33 antibody at 1 ug/mL. Mouse IgG 12H6D12 Aff - Purified Hu, Ms E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-33 (IL33) antibody

This antibody stained formalin-fixed, paraffin-embedded sections of human breast malignant ductal adenocarcinoma.  The recommended concentration is 0.125 ug/ml-0.25 ug/ml with an overnight incubation at 4C.  An HRP-labeled polymer detection system was used with a DAB chromogen.  Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended.  Optimal concentrations and conditions may vary. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-33 (IL33) antibody

Western Blot: IL33 Antibody (6H496) [TA337187] - Analysis of IL-33 using IL-33 monoclonal antibody in A. HUVEC cell lysate at 10 ug/ml and B. partial recombinant IL-33 (amino acids 112-270) at 1 ug/ml. Goat anti-mouse Ig HRP secondary antibody. Mouse IgG1 6H496 Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-33 (IL33) antibody

Western Blot: IL33 Antibody (1C5) [TA337195] - IL33 Antibody (1C5) [TA337195] - WB analysis of IL33 recombinant protein using IL33 antibody (clone 1C5) at a concentration of 2 ug/ml. Mouse IgG2b 1C5 Purified Hu, Ms C, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-33 (IL33) antibody

WB Suggested Anti-IL33 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: 293T cell lysate Rabbit IgG Purified Hu P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Interleukin-33 (IL33) antibody

The Cell lysates of Jurkat (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human IL-33 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG2b 4E9 Purified Hu E, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

Interleukin-33 (IL33) antibody

The Cell lysates of Jurkat (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human IL-33 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG2b 4E9 Purified Hu E, WB
50 µl / €230.00
  Acris Antibodies GmbH

Interleukin-33 (IL33) antibody

Interleukin-33 (IL33) Mouse IgG1 B-L33 Azide Free Hu
0.5 mg / €460.00
  Acris Antibodies GmbH

Interleukin-33 (IL33) antibody

Interleukin-33 (IL33) Mouse IgG1 B-S33 Azide Free Hu
0.5 mg / €460.00
  Acris Antibodies GmbH

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