Interleukin-33 (IL33) antibody

Principal name

Interleukin-33 (IL33) antibody

Alternative names for Interleukin-33 (IL33) antibody

IL-33, IL1F11, IL-1F11, NFHEV, NF-HEV, C9orf26, Interleukin-1 family member 11, Nuclear factor from high endothelial venules

SwissProt ID

O95760 (Human), O97863 (Canfa), Q66H70 (Rat), Q8BVZ5 (Mouse)

Gene ID

90865 (IL33), 77125 (Il33), 361749

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Functional assay (FN), Frozen Sections (C)

Background of Interleukin-33 (IL33) antibody

Interleukin-33 (IL-33) is a recently identified member of the IL-1 family of cytokines whose other members include IL-1a/b, IL-1Ra and IL-18. Its receptor has been shown to be ST2, an IL-1 receptor family member that also acts as a negative regulator of TLR-IL-1R signaling and IL-1R accessory protein (IL-1RAcP). Receptor binding of IL-33 activates NF-kappaB and MAP kinases and induces the expression of TH2-associated cytokines such as IL-4, IL-5 and IL-6. Prolonged IL-33 treatment of mice led to the development of eosinophilia, splenomegaly, and severe pathological changes in mucosal organs such as lungs, esophagus and small intestine. Recent experiments have shown that IL-33 can also co-localize with heterochromatin and possesses transcriptional repressor activities, indicating that IL-33 may function as both a proinflammatory cytokine and an intracellular nuclear factor with transcriptional regulatory properties. Despite its predicted molecular weight, IL-33 will often run at higher molecular weight in SDS-PAGE.

General readings

1. Schmitz J, Owyang A, Oldham E, et al. IL-33, and interleukin-1-like cytokine that signals via the IL-1 receptor-related protein ST2 and induces T helper type 2-associated cytokines. Immunity 2005; 23:479-90.
2. Dinarello CA. Interleukin-18, a proinflammatory cytokine. Eur. Cytokine Netw. 2000; 11:483-6.
3. Brint EK, Xu D, Liu H, et al. ST2 is an inhibitor of interleukin 1 receptor and Toll-like receptor 4 signaling and maintains endotoxin tolerance. Nat. Immunol. 2004; 5:373-9.
4. Chackerian AA, Oldham ER, Murphy EE, et al. IL-1 receptor
accessory protein and ST2 comprise the IL-33 receptor complex. J. Immunol. 2007; 179:2551-5.
5. Carriere V, Roussel L, Ortega N, et al. IL-33, the IL-1-like cytokine ligand for ST2 receptor, is a chromatin-associated nuclear factor in vivo. Proc. Natl. Acad. Sci. USA 2007; 104:282-7.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Interleukin-33 (IL33) antibody

Western Blot unreduced using Interleukin-33 antibody Cat.-No AP32086BT Rabbit Biotin Hu E, WB
50 µg / €340.00
  Acris Antibodies GmbH

Interleukin-33 (IL33) antibody

Western Blot unreduced using Interleukin-33 antibody Cat.-No AP32086BT Rabbit Biotin Hu E, WB
25 µg / €230.00
  Acris Antibodies GmbH

Interleukin-33 (IL33) antibody

Formalin-Fixed, Paraffin-Embedded Sections of human breast malignant ductal adenocarcinoma stained with Interleukin-33 antibody Cat.-No AP32086PU Rabbit Aff - Purified Hu E, FN, P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

Interleukin-33 (IL33) antibody

Formalin-Fixed, Paraffin-Embedded Sections of human breast malignant ductal adenocarcinoma stained with Interleukin-33 antibody Cat.-No AP32086PU Rabbit Aff - Purified Hu E, FN, P, WB
50 µg / €230.00
  Acris Antibodies GmbH

Interleukin-33 (IL33) (165-178) antibody

AP32727PU-N IL33 Antibody staining of Jurkat lysate at 1µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Interleukin-33 (IL33) (N-term) antibody

  Rabbit Polyclonal IL-33 Antibody  
Western blot analysis of IL-33 in human lymph node tissue lysate with IL-33 antibody at (A) 1 and (B) 2 ug/mL. Rabbit IgG Aff - Purified Hu E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-33 (IL33) (Center) antibody

  Rabbit Polyclonal IL-33 Antibody  
Western blot analysis of IL-33 in human lymph node tissue lysate with IL-33 antibody at (A) 1 and (B) 2 µg/ml. Rabbit IgG Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-33 (IL33) antibody

  Rabbit polyclonal IL-33 antibody Peroxidase Conjugated  
Western blot using HRP conjugated anti-Human IL-33 antibody shows detection of a band ~18 kDa in size corresponding to recombinant human IL-33. The identity of the higher molecular weight band is unknown. Molecular weight markers are shown (left). After transfer, the membrane was blocked with 3% BSA in TBS followed by reaction with antibody at a 1:5,000 dilution for 30 min at room temperature. Detection occurred using TMB substrate. Rabbit IgG HRP Hu E, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

Interleukin-33 (IL33) antibody

  Rabbit polyclonal anti-IL-33 antibody  
WB using anti-Human IL-33 antibody shows detection of a band ~18 kDa in size corresponding to recom-binant human IL-33 (lane 1). The identity of the higher molecular weight band is unknown. Molecular weight markers are also shown (M). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution. Detection occurred using peroxidase conjugated anti-Rabbit IgG (p/n 611-103-122) secondary antibody diluted 1:40,000. Rabbit IgG Ig Fraction Hu E, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

Interleukin-33 (IL33) antibody

  Rabbit polyclonal IL-33 antibody Biotin Conjugated  
WB using anti-Human IL-33 antibody shows detection of a band ~18 kDa in size corresponding to recom-binant human IL-33 (lane 1). The identity of the higher molecular weight band is unknown. Molecular weight markers are also shown (M). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution. Detection occurred using peroxidase conjugated anti-Rabbit IgG (p/n 611-103-122) secondary antibody diluted 1:40,000. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

Interleukin-33 (IL33) antibody

  Mouse IL-33 Monoclonal Antibody  
Western blot analysis of IL-33 using 125ng of recombinant IL-33 with IL-33 antibody at 1 ug/mL. Mouse IgG 12B3C4 Aff - Purified Hu, Ms E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-33 (IL33) antibody

  Mouse IL-33 Monoclonal Antibody  
Western blot analysis of IL-33 using 125ng of recombinant IL-33 with IL-33 antibody at 1 ug/mL. Mouse IgG 12H6D12 Aff - Purified Hu, Ms E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-33 (IL33) antibody

  Anti-Human IL-33 Rabbit Polyclonal Antibody  
This antibody stained formalin-fixed, paraffin-embedded sections of human breast malignant ductal adenocarcinoma.  The recommended concentration is 0.125 ug/ml-0.25 ug/ml with an overnight incubation at 4C.  An HRP-labeled polymer detection system was used with a DAB chromogen.  Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended.  Optimal concentrations and conditions may vary. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-33 (IL33) antibody

  Mouse Monoclonal IL-33 Antibody (6H496)  
Western Blot: IL33 Antibody (6H496) [TA337187] - Analysis of IL-33 using IL-33 monoclonal antibody in A. HUVEC cell lysate at 10 ug/ml and B. partial recombinant IL-33 (amino acids 112-270) at 1 ug/ml. Goat anti-mouse Ig HRP secondary antibody. Mouse IgG1 6H496 Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-33 (IL33) antibody

  Mouse Monoclonal IL-33 Antibody (1C5)  
Western Blot: IL33 Antibody (1C5) [TA337195] - IL33 Antibody (1C5) [TA337195] - WB analysis of IL33 recombinant protein using IL33 antibody (clone 1C5) at a concentration of 2 ug/ml. Mouse IgG2b 1C5 Purified Hu, Ms C, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-33 (IL33) antibody

  Rabbit Polyclonal Anti-IL33 Antibody  
WB Suggested Anti-IL33 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: 293T cell lysate Rabbit IgG Purified Hu P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Interleukin-33 (IL33) antibody

The Cell lysates of Jurkat (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human IL-33 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG2b 4E9 Purified Hu E, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

Interleukin-33 (IL33) antibody

The Cell lysates of Jurkat (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human IL-33 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG2b 4E9 Purified Hu E, WB
50 µl / €230.00
  Acris Antibodies GmbH

Interleukin-33 (IL33) antibody

Interleukin-33 (IL33) Mouse IgG1 B-L33 Azide Free Hu
0.5 mg / €460.00
  Acris Antibodies GmbH

Interleukin-33 (IL33) antibody

Interleukin-33 (IL33) Mouse IgG1 B-S33 Azide Free Hu
0.5 mg / €460.00
  Acris Antibodies GmbH

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