Interleukin-4 / IL4 antibody

Principal name

Interleukin-4 / IL4 antibody

Alternative names for Interleukin-4 / IL4 antibody

IL-4, BSF1, Lymphocyte stimulatory factor 1, Binetrakin, Pitrakinra, B-cell stimulatory factor 1

SwissProt ID

O77762 (Canfa), P05112 (Human), P07750 (Mouse), P20096 (Rat), P30367 (Bovin), P30368 (Sheep), P42202 (Horse), P55030 (Felca), Q04745 (Pig), Q60440 (Mesau), Q9MZR8 (Rabit)

Gene ID

3565 (IL4), 16189 (Il4), 287287, 280824, 397225

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Gt (Goat), Sh (Sheep), Por (Porcine), Can (Canine), Md (Mustelid), Mky (Monkey)

Available hosts

Mouse, Rabbit, Rat, Goat, Sheep

Available applications

Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Frozen Sections (C), Functional assay (FN), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (capture) (E(capture)), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Dot blot (Dot), Immunoprecipitation (IP), Neutralisation (NEUT), Luminex (LMNX)

Background of Interleukin-4 / IL4 antibody

Interleukin 4 (IL-4) is a cytokine that regulates the development, proliferation and maturation of B and T-lymphocytes, as well as non-leukocyte somatic cells. IL-4 promotes antigen presentation on B cells by enhancing expression of class II MHC molecules and the co-stimulatory molecules CD80 and CD86. IL-4 can also enhance the secretion and cell surface expression of IgE and IgG1 on B-cells. The expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes is regulated by IL-4. IL-4 is produced by a number of hematopoietic cell types including T-cells (predominantly TH2 cells), CD3+ NK-T cells, mast cells, eosinophils, and basophils. Human IL-4 is synthesized as a precursor protein of 153 amino acids, which includes a hydrophobic signal sequence of 24 amino acids that is cleaved upon secretion from the cell. Biological activity of IL-4 is dependent on the formation of 3 disulfide bridges and its structure is related to that of GM-CSF, M-CSF, and growth hormone. This is a HCX protein. HCX Expression System Details HCX proteins mimic the proteins in the human body because they are expressed from human, rather than animal, insect or bacterial cells. This process gives them human post-translational modifications. Recombinant DNA techniques allow a human protein with the correct amino acid sequence to be expressed in a non-human cell line. However, non-human cells lack the appropriate cellular machinery, such as specific glycosyltransferases, necessary to produce the correct human post-translational modifications of a protein. An extreme example is seen in E. coli cells, which produce recombinant proteins with no glycosylation, as the above figure illustrates. Rodent and yeast cells are able to glycosylate proteins, but they are still different from glycosylation in human cells. Expression System Resultant Proteins Human (e.g. K562, HEK293) Correct amino acid sequence Human post-translational modifications Rodent (e.g. CHO, NSO) Correct amino acid sequence Some natural glycosylation - not human-like Yeast (e.g. Pichia) Correct amino acid sequence Some natural glycosylation - not human-like E.Coli Correct amino acid sequence No PTMs Although there have been significant attempts to make non-human cell derived cytokines more human-like, there is a growing awareness that in many instances, particularly in therapeutics, cytokines should mimic those found in the body as closely as possible.

General readings

Kay NE & Pittner BT (2003) Leuk Lymphoma. 44(6): 897-903

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Interleukin-4 / IL4 antibody

Western blot analysis of extracts from HeLa cells, using IL4 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Interleukin-4 / IL4 antibody

Western blot analysis using recombinant human IL-4 Lane 1: 50 ng, Lane 2: 100 ng. Rabbit Purified Hu WB
0.2 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-4 / IL4 antibody

anti-Human IL-4 antibody shows detection of a band ~15 kDa in size corresponding to recombinant human IL-4. The identity of the faint higher molecular weight band may represent a homodimer. Molecular weight markers are also shown (left). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution. Detection occurred using peroxidase conjugated anti-Rabbit IgG (p/n 611-103-122) secondary antibody diluted 1:40,000. Rabbit IgG Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-4 / IL4 antibody

Western Blot of Rabbit Anti - IL-4 Antibody Peroxidase Conjugated Lane 1: Human IL-4 Load: 50 ng per lane Secondary antibody: IL-4 Antibody Peroxidase Conjugated at 1:1,000 for 60 min at RT Block: MB-070 for 30 min RT Predicted/Observed size: 18 kDa, 18 kDa Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-4 / IL4 antibody

Western Blot of Biotin Conjugated Rabbit anti-IL-4 Antibody. Lane 1: Human IL-4. Lane 2: none. Load: 50 ng per lane. Primary antibody: IL-4 Antibody Biotin Conjugated at 1:1,000 for overnight at 4°C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 17 kDa, 17 kDa for Human IL-4. Other band(s): none. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-4 / IL4 antibody

Splenocytes from 8 week old mice pre-immunized with OVA/IFA were recall-activated with OVA antigen for 36 hours in Mouse IL-4 ELISPOT assay.  Control well is medium alone. Rat 11B11 Purified Ms E
50 µg / €120.00
  OriGene Technologies, Inc.

Interleukin-4 / IL4 antibody

Interleukin-4 / IL4 Mouse 8D4-8 Purified Hu E
50 µg / €120.00
  OriGene Technologies, Inc.

Interleukin-4 / IL4 antibody

(LEFT)Western blot analysis of IL4 Antibody (C-term) (Cat. #TA324803) in WiDr cell line lysates (35ug/lane).IL4 (arrow) was detected using the purified Pab.(RIGHT)Western blot analysis of IL4 Antibody (C-term) (Cat. #TA324803) in mouse cerebellum cell line lysates (35ug/lane).IL4(arrow) was detected using the purified Pab. Rabbit IgG Purified Hu, Mky, Ms F, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

Interleukin-4 / IL4 antibody

To detect hIL-4 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-4. Goat IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Interleukin-4 / IL4 antibody

To detect hIL-4 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-4 (TA328210) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-4. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-4 / IL4 antibody

To detect Murine IL-4 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Murine IL-4. Rabbit IgG Purified E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Interleukin-4 / IL4 antibody

To detect Murine IL-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Murine IL-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. Rabbit IgG Purified E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-4 / IL4 antibody

To detect Rat IL-4 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat IL-4. Rabbit IgG Purified Rt E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Interleukin-4 / IL4 antibody

To detect Rat IL-4 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Rat IL-4 (TA328570) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-4. Rabbit IgG Purified Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-4 / IL4 antibody

WB Suggested Anti-IL4 Antibody Titration: 0.2-1 ug/ml; Positive Control: Hela cell lysate Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

Interleukin-4 / IL4 antibody

WB Suggested Anti-IL4 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Human Spleen Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

Interleukin-4 / IL4 antibody

Western blot analysis of Interleukin 4 expression in MCF-7cells Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-4 / IL4 antibody

IL-4 ELISA with 8D4 Capture (TA600022) and 25D2 Detection (TA700022) Antibodies. Substrate used: Recombinant Human IL-4 (E. Coli expressed Peptide) Mouse IgG1 OTI8D4 Purified Hu E, LMNX
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-4 / IL4 antibody

IL-4 ELISA with 8D4 Capture (TA600022) and 25D2 Detection (TA700022) Antibodies. Substrate used: Recombinant Human IL-4 (E. Coli expressed Peptide) Rat IgG1 OTI25D2 Purified Hu E, LMNX
50 µg / €345.00
  OriGene Technologies, Inc.

Interleukin-4 / IL4 antibody

Interleukin-4 / IL4 Rat IgG1 I4.F5 Azide Free Ms FN, WB
0.2 mg / €760.00
  Acris Antibodies GmbH

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