Interleukin-4 / IL4 antibody

Principal name

Interleukin-4 / IL4 antibody

Alternative names for Interleukin-4 / IL4 antibody

IL-4, BSF1, Lymphocyte stimulatory factor 1, Binetrakin, Pitrakinra, B-cell stimulatory factor 1

SwissProt ID

O77762 (Canfa), P05112 (Human), P07750 (Mouse), P20096 (Rat), P30367 (Bovin), P30368 (Sheep), P42202 (Horse), P55030 (Felca), Q04745 (Pig), Q60440 (Mesau), Q9MZR8 (Rabit)

Gene ID

3565 (IL4), 16189 (Il4), 287287, 280824

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Gt (Goat), Por (Porcine), Sh (Sheep), Md (Mustelid), Rt (Rat), Mky (Monkey)

Available hosts

Mouse, Rat, Rabbit, Sheep, Goat

Available applications

Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Functional assay (FN), Western blot / Immunoblot (WB), Flow Cytometry (F), ELISA (capture) (E(capture)), Immunoprecipitation (IP), Neutralisation (NEUT), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Paraffin Sections (P), Luminex (LMNX)

Background of Interleukin-4 / IL4 antibody

Interleukin 4 (IL-4) is a pleiotropic cytokine produced primarily by activated T lymphocytes, mast cells and basophils .

The cDNA sequence of mouse IL-4 predicts a 140 amino acid (aa) residue precursor protein containing a 20 aa residue signal peptide that is cleaved to form the mature protein . At the amino acid sequence level, mature mouse IL-4 is approximately 50% identical to human IL-4 but there is no species cross-reactivity for biological activity for the two proteins . Mouse IL-4 also shares approximately 30% amino acid sequence identity to mouse IL-13 and the two cytokines exhibit overlapping biological activities . The gene for IL-4 has been mapped to mouse chromosome 11, in close proximity to the genes for IL-3, IL-5, IL-13 and GM-CSF .

IL-4 has multiple immune response-modulating activities on a variety of cell types. It is an important regulator of isotype switching, inducing IgE production in B lymphocytes. It is an important modulator of the differentiation of precursor T helper cells to the Th2 subset that mediates humoral immunity and modulates antibody production. In addition, IL-4 has also been shown to have anti-tumor activity both in vivo and in vitro .

The biological effects of IL-4 are mediated by specific cell surface receptor complexes. Although IL-4 R does not bind IL-13 directly, it has been shown to complex with the low-affinity IL-13 R to form the functional high-affinity receptor complex for IL-13 . In addition to the membrane-bound form of IL-4 R, a naturally occurring soluble form of IL-4 R has been identified in human and mouse biological fluids and in mouse cell culture supernates . Soluble IL-4 R has been to shown to bind IL-4 with high affinity in solution.

General readings

1. Abrams JS, Roncarolo MG, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992 Jun;127:5-24. PubMed PMID: 1387110.
2. Bogen SA, Fogelman I, Abbas AK. Analysis of IL-2, IL-4, and IFN-gamma-producing cells in situ during immune responses to protein antigens. J Immunol. 1993 May 15;150(10):4197-205. PubMed PMID: 8482832.
3. Fujihashi K, McGhee JR, Beagley KW, McPherson DT, McPherson SA, Huang CM, et al. Cytokine-specific ELISPOT assay. Single cell analysis of IL-2, IL-4 and IL-6 producing cells. J Immunol Methods. 1993 Apr 2;160(2):181-9. PubMed PMID: 8459105.
4. Ferrick DA, Schrenzel MD, Mulvania T, Hsieh B, Ferlin WG, Lepper H. Differential production of interferon-gamma and interleukin-4 in response to Th1- and Th2-stimulating pathogens by gamma delta T cells in vivo. Nature. 1995 Jan 19;373(6511):255-7. PubMed PMID: 7816142.
5. Chiu, W.C. et al. (2009) Effects of dietary fish oil supplementation on cellular adhesion molecule expression and tissue myeloperoxidase activity in hypercholesterolemic mice with sepsis. J Nutr Biochem. 2009 Apr;20(4):254-60.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Interleukin-4 / IL4 antibody

  Rabbit polyclonal anti-IL4 antibody  
Western blot analysis of extracts from HeLa cells, using IL4 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Interleukin-4 / IL4 antibody

  Rabbit polyclonal anti-IL-4 antibody  
Western blot analysis using recombinant human IL-4 Lane 1: 50 ng, Lane 2: 100 ng. Rabbit Purified Hu WB
0.2 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-4 / IL4 antibody

  Rabbit polyclonal anti-IL-4 antibody  
anti-Human IL-4 antibody shows detection of a band ~15 kDa in size corresponding to recombinant human IL-4. The identity of the faint higher molecular weight band may represent a homodimer. Molecular weight markers are also shown (left). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution. Detection occurred using peroxidase conjugated anti-Rabbit IgG (p/n 611-103-122) secondary antibody diluted 1:40,000. Rabbit IgG Purified Hu E, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

Interleukin-4 / IL4 antibody

  Rabbit polyclonal IL-4 antibody Peroxidase Conjugated  
Western Blot of Rabbit Anti - IL-4 Antibody Peroxidase Conjugated Lane 1: Human IL-4 Load: 50 ng per lane Secondary antibody: IL-4 Antibody Peroxidase Conjugated at 1:1,000 for 60 min at RT Block: MB-070 for 30 min RT Predicted/Observed size: 18 kDa, 18 kDa Rabbit IgG Purified Hu E, P, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

Interleukin-4 / IL4 antibody

  Rabbit polyclonal IL-4 antibody Biotin Conjugated  
Western Blot of Biotin Conjugated Rabbit anti-IL-4 Antibody. Lane 1: Human IL-4. Lane 2: none. Load: 50 ng per lane. Primary antibody: IL-4 Antibody Biotin Conjugated at 1:1,000 for overnight at 4°C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 17 kDa, 17 kDa for Human IL-4. Other band(s): none. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

Interleukin-4 / IL4 antibody

  Rat Anti-Mouse IL-4 Purified (50 ug)  
Splenocytes from 8 week old mice pre-immunized with OVA/IFA were recall-activated with OVA antigen for 36 hours in Mouse IL-4 ELISPOT assay.  Control well is medium alone. Rat 11B11 Purified Ms E
50 µg / €120.00
  OriGene Technologies, Inc.

Interleukin-4 / IL4 antibody

  Mouse Anti-Human IL-4 Purified (50 ug)  
Interleukin-4 / IL4 Mouse 8D4-8 Purified Hu E
50 µg / €120.00
  OriGene Technologies, Inc.

Interleukin-4 / IL4 antibody

  Rabbit polyclonal IL4 Antibody (C-term)  
(LEFT)Western blot analysis of IL4 Antibody (C-term) (Cat. #TA324803) in WiDr cell line lysates (35ug/lane).IL4 (arrow) was detected using the purified Pab.(RIGHT)Western blot analysis of IL4 Antibody (C-term) (Cat. #TA324803) in mouse cerebellum cell line lysates (35ug/lane).IL4(arrow) was detected using the purified Pab. Rabbit IgG Purified Hu, Mky, Ms F, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

Interleukin-4 / IL4 antibody

  Biotinylated Anti-Human IL-4 Goat Polyclonal Antibody  
To detect hIL-4 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-4. Goat IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Interleukin-4 / IL4 antibody

  Anti-Human IL-4 Rabbit Polyclonal Antibody  
To detect hIL-4 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-4 (TA328210) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-4. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-4 / IL4 antibody

  Biotinylated Anti-Murine IL-4 Rabbit Polyclonal Antibody  
To detect Murine IL-4 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Murine IL-4. Rabbit IgG Purified E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Interleukin-4 / IL4 antibody

  Anti-Murine IL-4 Rabbit Polyclonal Antibody  
To detect Murine IL-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Murine IL-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. Rabbit IgG Purified E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-4 / IL4 antibody

  Biotinylated Anti-Rat IL-4 Rabbit Polyclonal Antibody  
To detect Rat IL-4 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat IL-4. Rabbit IgG Purified Rt E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Interleukin-4 / IL4 antibody

  Anti-Rat IL-4 Rabbit Polyclonal Antibody  
To detect Rat IL-4 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Rat IL-4 (TA328570) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-4. Rabbit IgG Purified Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-4 / IL4 antibody

  Rabbit Polyclonal Anti-IL4 Antibody  
WB Suggested Anti-IL4 Antibody Titration: 0.2-1 ug/ml; Positive Control: Hela cell lysate Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

Interleukin-4 / IL4 antibody

  Rabbit Polyclonal Anti-IL4 Antibody  
WB Suggested Anti-IL4 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Human Spleen Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

Interleukin-4 / IL4 antibody

  Rabbit Polyclonal Anti-Interleukin 4 Antibody  
Western blot analysis of Interleukin 4 expression in MCF-7cells Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-4 / IL4 antibody

  Mouse Monoclonal Anti-IL-4 Antibody  
Interleukin-4 / IL4 Mouse IgG1 DL-8 Purified Rt E, Elispot
0.5 mg / €413.00
  OriGene Technologies, Inc.

Interleukin-4 / IL4 antibody

  Mouse Monoclonal Anti-IL-4 Antibody  
Interleukin-4 / IL4 Mouse IgG1 OX-81 Purified Rt E, Elispot
0.5 mg / €413.00
  OriGene Technologies, Inc.

Interleukin-4 / IL4 antibody

  Rabbit Polyclonal Anti-IL-4 Antibody  
Interleukin-4 / IL4 Rabbit IgG Purified Rt E, Elispot, WB
0.5 mg / €303.00
  OriGene Technologies, Inc.

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