Interleukin-5 / IL5 antibody

Principal name

Interleukin-5 / IL5 antibody

Alternative names for Interleukin-5 / IL5 antibody

IL-5, B-cell differentiation factor I, Eosinophil differentiation factor, T-cell replacing factor, TRF

SwissProt ID

O02699 (Horse), O08987 (Cavpo), O77515 (Felca), P04401 (Mouse), P05113 (Human), P52173 (Bovin), Q08125 (Rat), Q28586 (Sheep), Q95J76 (Canfa), Q9MYM5 (Pig)

Gene ID

3567 (IL5), 16191 (Il5), 24497

Available reactivities

Hu (Human), GP (Guinea Pig), Ms (Mouse), Rt (Rat), Rb (Rabbit)

Available hosts

Rabbit, Rat, Mouse, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Functional assay (FN), Paraffin Sections (P), Flow Cytometry (F), ELISA (capture) (E(capture)), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Frozen Sections (C), Neutralisation (NEUT)

Background of Interleukin-5 / IL5 antibody

Interleukin 5 (IL-5) plays an important role in B cell development by inducing the terminal differentiation of late-developing B cells. Additionally, IL-5 regulates the differentiation, survival and degranulation of eosinophils. IL-5 is expressed predominately by T-lymphocytes and mast cells, and to a lesser extent by eosinophils, natural killer cells and endothelial cells. IL-5 is a 115 amino acid glycoprotein that exists as a disulfide linked homodimer. This is a HCX protein. HCX Expression System Details HCX proteins mimic the proteins in the human body because they are expressed from human, rather than animal, insect or bacterial cells. This process gives them human post-translational modifications. Recombinant DNA techniques allow a human protein with the correct amino acid sequence to be expressed in a non-human cell line. However, non-human cells lack the appropriate cellular machinery, such as specific glycosyltransferases, necessary to produce the correct human post-translational modifications of a protein. An extreme example is seen in E. coli cells, which produce recombinant proteins with no glycosylation, as the above figure illustrates. Rodent and yeast cells are able to glycosylate proteins, but they are still different from glycosylation in human cells. Expression System Resultant Proteins Human (e.g. K562, HEK293) Correct amino acid sequence Human post-translational modifications Rodent (e.g. CHO, NSO) Correct amino acid sequence Some natural glycosylation - not human-like Yeast (e.g. Pichia) Correct amino acid sequence Some natural glycosylation - not human-like E.Coli Correct amino acid sequence No PTMs Although there have been significant attempts to make non-human cell derived cytokines more human-like, there is a growing awareness that in many instances, particularly in therapeutics, cytokines should mimic those found in the body as closely as possible.

General readings

Mordvinov VA, et al., (2001) Arch Immunol Ther Exp. 49(5): 345-51.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Interleukin-5 / IL5 antibody

To detect Human IL-5 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Human IL-5. Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Interleukin-5 / IL5 antibody

To detect Human IL-5 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-5 (TA328212) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL-5. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-5 / IL5 antibody

To detect Murine IL-5 by direct ELISA (using 100  ml/well) a concentration of approximately 1.0  ug/ml of this antibody is required. This  biotinylated polyclonal antibody allows the detection of at least 0.2-0.4  ng/well of recombinant Murine IL-5. Rabbit IgG Purified E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Interleukin-5 / IL5 antibody

To detect Murine IL-5 by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine IL-5 (TA328486) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Murine IL-5. Rabbit IgG Purified E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-5 / IL5 antibody

WB Suggested Anti-Il5 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Mouse Spleen Rabbit IgG Purified GP, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

Interleukin-5 / IL5 antibody

WB Suggested Anti-IL5 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Hela cell lysate Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

Interleukin-5 / IL5 antibody

Western blot analysis of Interleukin 5 expression in HT-29 cells Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-5 / IL5 antibody

Interleukin-5 / IL5 Rat IgG2a JES1-5A10 Biotin Hu E, E(capture), E(detection)
0.5 mg / €330.00
  Acris Antibodies GmbH

Interleukin-5 / IL5 antibody

Interleukin-5 / IL5 Rat IgG2a JES1-5A10 Purified Hu E, E(capture), E(detection)
0.5 mg / €320.00
  Acris Antibodies GmbH

Interleukin-5 / IL5 antibody

Western blot (WB) analysis of IL-5 antibody (Cat.-No.: AP20595PU-N) in extracts from HT-29 cells. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Interleukin-5 / IL5 antibody

Interleukin-5 / IL5 Rabbit Biotin Ms E, WB
50 µg / €340.00
  Acris Antibodies GmbH

Interleukin-5 / IL5 antibody

Interleukin-5 / IL5 Rabbit Biotin Ms E, WB
25 µg / €230.00
  Acris Antibodies GmbH

Interleukin-5 / IL5 antibody

Interleukin-5 / IL5 Rabbit Aff - Purified Ms E, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

Interleukin-5 / IL5 antibody

Interleukin-5 / IL5 Rabbit Aff - Purified Ms E, WB
50 µg / €230.00
  Acris Antibodies GmbH

Interleukin-5 / IL5 antibody

Interleukin-5 / IL5 Rabbit Biotin Hu E, WB
25 µg / €230.00
  Acris Antibodies GmbH

Interleukin-5 / IL5 antibody

Interleukin-5 / IL5 Rabbit Biotin Hu E, WB
50 µg / €340.00
  Acris Antibodies GmbH

Interleukin-5 / IL5 antibody

Immunohistochemistry: The Interleukin-5 antibody stained formalin-fixed, paraffin-embedded sections of human colon/rectum adenocarcinoma. The recommended concentration is 0.25µg/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen.  Optimal results for these conditions were achieved without antigen retrieval. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. Rabbit Aff - Purified Hu E, FN, P, WB
50 µg / €230.00
  Acris Antibodies GmbH

Interleukin-5 / IL5 antibody

Immunohistochemistry: The Interleukin-5 antibody stained formalin-fixed, paraffin-embedded sections of human colon/rectum adenocarcinoma. The recommended concentration is 0.25µg/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen.  Optimal results for these conditions were achieved without antigen retrieval. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. Rabbit Aff - Purified Hu E, FN, P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

Interleukin-5 / IL5 antibody

Interleukin-5 / IL5 Rat IgG1 TRFK5 Purified GP, Hu, Ms E, F, WB
0.5 mg / €450.00
  Acris Antibodies GmbH

Interleukin-5 / IL5 antibody

Interleukin-5 / IL5 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

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