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Interleukin-5 / IL5 antibody

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Principal name

Interleukin-5 / IL5 antibody

Alternative names for Interleukin-5 / IL5 antibody

IL-5, B-cell differentiation factor I, Eosinophil differentiation factor, T-cell replacing factor, TRF

SwissProt ID

O02699 (Horse), O08987 (Cavpo), O77515 (Felca), P04401 (Mouse), P05113 (Human), P52173 (Bovin), Q08125 (Rat), Q28586 (Sheep), Q95J76 (Canfa), Q9MYM5 (Pig)

Gene ID

3567 (IL5), 16191 (Il5), 24497

Ncbi ID

NP_000870.1, 9606, 10090, 10116, NP_000870, NP_034688.1, NP_034688, NM_000879, NM_010558

Available reactivities

Hu (Human), GP (Guinea Pig), Ms (Mouse), Rt (Rat), Rb (Rabbit), Macaque

Available hosts

Rabbit, Rat, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Functional assay (FN), Paraffin Sections (P), Flow Cytometry (F), ELISA (capture) (E(capture)), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)

Background of Interleukin-5 / IL5 antibody

Interleukin-5(IL-5)is a secreted glycoprotein that belongs to the .-helical group of cytokines . Unlike other family members, it is present as a covalently linked antiparallel dimer . The cDNA for human IL-5 encodes a signal peptide and a 115 amino acid (aa) mature protein. Mature human IL-5 shares 70%, 70%, 62%, 71%, 70% and 66%, aa sequence identity with mouse, rat, canine, equine, feline and porcine IL-5, respectively and shows cross-reactivity with mouse IL-5. IL-5 is primarily produced by CD4+ Th2 cells, but also by activated eosinophils, mast cells, EBV-transformed B cells, Reed-Sternberg cells in HodgkinÂ?s disease, and IL-2-stimulated invariant natural killer T cells (iNKT) (1-3, 6-8). IL-5 increases production and mobilization of eosinophils and CD34+ progenitors from the bone marrow and causes maturation of eosinophil precursors outside the bone marrow . The receptor for human IL-5, mainly expressed by eosinophils, but also found on basophils and mast cells, consists of a unique ligand-binding subunit (IL-5 R.) and a shared Signaltransducing subunit, .c . IL-5 R. first binds IL-5 at low affinity, then associates with preformed .c dimers, forming a high-affinity receptor . IL-5 also binds proteoglycans, potentially enhancing its activity . Soluble forms of IL-5 R. antagonize IL-5 and can be found in vivo . In humans, IL-5 primarily affects cells of the eosinophilic lineage, and promotes their differentiation, maturation, activation, migration and survival, while in mice IL-5 also enhances Ig class switching and release from B1 cells (1-3, 9, 10, 15). IL-5 also promotes differentiation of basophils and primes them for histamine and leukotriene release .

General readings

Rosenberg, H. F. et al. (2007) J. Allergy Clin. Immunol.119:1303.

Elsas, P.X. and M. I. G. Elsas (2007) Curr. Med. Chem.14:1925.

MartinezMoczygemba,M. and D. P. Huston (2003) J. Allergy Clin. Immunol. 112:653.

Minamitake, Y. et al. (1990) J. Biochem. 107:292.

McKenzie, A. N. et al. (1991) Mol. Immunol. 28:155.

Shakoory, B. et al. (2004) J. Interferon Cytokine Res. 24:271.

Lalani, T. et al. (1999) Ann. Allergy Asthma Immunol. 82:317.

Sakuishi, K. et al. (2007) J. Immunol. 179:3452.

Clutterbuck, E. J. et al. (1989) Blood 73:1504.

Cameron, L. et al. (2000) J. Immunol. 164:1538.

Tavernier, J. et al. (1991) Cell 66:1175.

ZaksZilberman, M. et al. (2008) J. Biol. Chem. 283:13398.

Lipscombe, R. et al. (1998) J. Leukocyte Biol. 63:342.

Tavernier, J. et al. (2000) Blood 95:1600.

Kopf, M. et al. (1996) Immunity 4:15.

Denburg, J. A. et al. (1991) Blood 77:1462.

PP1027P1 - Interleukin-5 / IL5
Immunohistochemistry: The Interleukin-5 antibody stained formalin-fixed, paraffin-embedded sections of human colon/rectum adenocarcinoma. The recommended concentration is 0.25µg/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results for these conditions were achieved without antigen retrieval. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute.
PP1027P1 - Interleukin-5 / IL5
Sandwich ELISA using Interleukin-5 / IL5 Antibody Cat.-No PP1027P.
ARP54360_P050 - Interleukin-5 / IL5
Human HeLa; WB Suggested Anti-IL5 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Hela cell lysate; IL5 antibody - middle region (ARP54360_P050) in Human HeLa cells using Western Blot
AP20595PU-N - Interleukin-5 / IL5
Western blot (WB) analysis of IL-5 antibody (Cat.-No.: AP20595PU-N) in extracts from HT-29 cells.
AP20595PU-N - Interleukin-5 / IL5
Immunohistochemistry (IHC) analyzes of IL-5 antibody (Cat.-No.: AP20595PU-N) in paraffin-embedded human breast carcinoma tissue.
ARP54359_P050 - Interleukin-5 / IL5
Mouse Spleen; WB Suggested Anti-Il5 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Mouse Spleen; Il5 antibody - middle region (ARP54359_P050) in Mouse Spleen cells using Western Blot
CPA1593-100ul - Interleukin-5 / IL5
Immunohistochemical analysis of IL-5 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
CPA1593-100ul - Interleukin-5 / IL5
Western blot analysis of IL-5 expression in HeLa (A); HepG2 (B) whole cell lysates.
CPA1594-100ul - Interleukin-5 / IL5
Western blot analysis of IL-5 expression in MCF7 (A); SP2/0 (B); H9C2 (C) whole cell lysates.
CEK1237 - Human IL-5 ELISA Kit
Representative Standard Curve
CEK1238 - Human IL-5 ELISA Kit
Representative Standard Curve
CEK1512 - Mouse IL-5 ELISA Kit
Representative Standard Curve
CEK1513 - Mouse IL-5 ELISA Kit
Representative Standard Curve
TP723237 - Interleukin-5 / IL5
TP723787 - Interleukin-5 / IL5
TA328212 - Interleukin-5 / IL5
To detect Human IL-5 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Human IL-5.
TA328212 - Interleukin-5 / IL5
To detect Human IL-5 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human IL-5 (TA328213) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human IL-5.
TA328213 - Interleukin-5 / IL5
To detect Human IL-5 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-5 (TA328212) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL-5.
TA328213 - Interleukin-5 / IL5
To detect Human IL-5 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-5 (TA328212) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL-5.
TA328213 - Interleukin-5 / IL5
To detect Human IL-5 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-5 (TA328212) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL-5.
TA328486 - Interleukin-5 / IL5
To detect Murine IL-5 by direct ELISA (using 100 ml/well) a concentration of approximately 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody allows the detection of at least 0.2-0.4 ng/well of recombinant Murine IL-5.
TA328486 - Interleukin-5 / IL5
To detect Murine IL-5 by sandwich ELISA (using 100 ml/well) a concentration of 0.25-1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti- Murine IL-5 (TA328487) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Murine IL-5.
TA328487 - Interleukin-5 / IL5
To detect Murine IL-5 by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine IL-5 (TA328486) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Murine IL-5.
TA328487 - Interleukin-5 / IL5
To detect Murine IL-5 by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine IL-5 (TA328486) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Murine IL-5.
TA335656 - Interleukin-5 / IL5
WB Suggested Anti-Il5 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Mouse Spleen
TA336214 - Interleukin-5 / IL5
WB Suggested Anti-IL5 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Hela cell lysate
TA347874 - Interleukin-5 / IL5
Western blot analysis of Interleukin 5 expression in HT-29 cells

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Interleukin-5 / IL5 antibody
  Biotinylated Anti-Human IL-5 Rabbit Polyclonal Antibody  
To detect Human IL-5 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Human IL-5. Rabbit IgG Purified Hu E, WB
50 µg / €335.00
  OriGene Technologies, Inc.
+1 additional image

Interleukin-5 / IL5 antibody
  Anti-Human IL-5 Rabbit Polyclonal Antibody  
To detect Human IL-5 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-5 (TA328212) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL-5. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €335.00
  OriGene Technologies, Inc.
+2 additional images

Interleukin-5 / IL5 antibody
  Biotinylated Anti-Murine IL-5 Rabbit Polyclonal Antibody  
To detect Murine IL-5 by direct ELISA (using 100 ml/well) a concentration of approximately 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody allows the detection of at least 0.2-0.4 ng/well of recombinant Murine IL-5. Rabbit IgG Purified E, WB
50 µg / €335.00
  OriGene Technologies, Inc.
+1 additional image

Interleukin-5 / IL5 antibody
  Anti-Murine IL-5 Rabbit Polyclonal Antibody  
To detect Murine IL-5 by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine IL-5 (TA328486) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Murine IL-5. Rabbit IgG Purified E, WB
0.1 mg / €335.00
  OriGene Technologies, Inc.
+1 additional image

Interleukin-5 / IL5 antibody
  Rabbit Polyclonal Anti-Il5 Antibody  
WB Suggested Anti-Il5 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Mouse Spleen Rabbit IgG Purified GP, Hu, Ms, Rb, Rt WB
50 µg / €360.00
  OriGene Technologies, Inc.

Interleukin-5 / IL5 antibody
  Rabbit Polyclonal Anti-IL5 Antibody  
WB Suggested Anti-IL5 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Hela cell lysate Rabbit IgG Purified Hu WB
50 µg / €360.00
  OriGene Technologies, Inc.

Interleukin-5 / IL5 antibody
  Rabbit Polyclonal Anti-Interleukin 5 Antibody  
Western blot analysis of Interleukin 5 expression in HT-29 cells Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €335.00
  OriGene Technologies, Inc.

Interleukin-5 / IL5 antibody
  Mouse Monoclonal Anti-IL-5 Antibody  
Interleukin-5 / IL5 Mouse IgG1 QS-5 Purified Hu, Macaque E, Elispot, WB
0.5 mg / €413.00
  OriGene Technologies, Inc.

Interleukin-5 / IL5 antibody
  Mouse Monoclonal Anti-IL-5 Antibody  
Interleukin-5 / IL5 Mouse IgG1 B-Z25 Purified Hu E, WB
0.5 mg / €413.00
  OriGene Technologies, Inc.

Interleukin-5 / IL5 antibody
Interleukin-5 / IL5 Rat IgG2a JES1-5A10 Biotin Hu E, E(capture), E(detection)
0.5 mg / €330.00
  OriGene Technologies GmbH

Interleukin-5 / IL5 antibody
Interleukin-5 / IL5 Rat IgG2a JES1-5A10 Purified Hu E, E(capture), E(detection)
0.5 mg / €320.00
  OriGene Technologies GmbH

Interleukin-5 / IL5 antibody
Western blot (WB) analysis of IL-5 antibody (Cat.-No.: AP20595PU-N) in extracts from HT-29 cells. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  OriGene Technologies GmbH
+1 additional image

Interleukin-5 / IL5 antibody
Interleukin-5 / IL5 Rabbit Biotin Ms E, WB
50 µg / €340.00
  OriGene Technologies GmbH

Interleukin-5 / IL5 antibody
Interleukin-5 / IL5 Rabbit Biotin Ms E, WB
25 µg / €230.00
  OriGene Technologies GmbH

Interleukin-5 / IL5 antibody
Interleukin-5 / IL5 Rabbit Aff - Purified Ms E, WB
0.1 mg / €340.00
  OriGene Technologies GmbH

Interleukin-5 / IL5 antibody
Interleukin-5 / IL5 Rabbit Aff - Purified Ms E, WB
50 µg / €230.00
  OriGene Technologies GmbH

Interleukin-5 / IL5 antibody
Interleukin-5 / IL5 Rabbit Biotin Hu E, WB
25 µg / €230.00
  OriGene Technologies GmbH

Interleukin-5 / IL5 antibody
Interleukin-5 / IL5 Rabbit Biotin Hu E, WB
50 µg / €340.00
  OriGene Technologies GmbH

Interleukin-5 / IL5 antibody
Immunohistochemistry: The Interleukin-5 antibody stained formalin-fixed, paraffin-embedded sections of human colon/rectum adenocarcinoma. The recommended concentration is 0.25µg/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results for these conditions were achieved without antigen retrieval. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. Rabbit Aff - Purified Hu E, FN, P, WB
50 µg / €230.00
  OriGene Technologies GmbH
+1 additional image

Interleukin-5 / IL5 antibody
Immunohistochemistry: The Interleukin-5 antibody stained formalin-fixed, paraffin-embedded sections of human colon/rectum adenocarcinoma. The recommended concentration is 0.25µg/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results for these conditions were achieved without antigen retrieval. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. Rabbit Aff - Purified Hu E, FN, P, WB
0.1 mg / €340.00
  OriGene Technologies GmbH
+1 additional image

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