Interleukin-6 / IL6 antibody

Principal name

Interleukin-6 / IL6 antibody

Alternative names for Interleukin-6 / IL6 antibody

IL-6, Interferon beta-2, IFNB2, B-cell stimulatory factor 2, BSF-2, CDF, Hybridoma growth factor, CTL differentiation factor

SwissProt ID

P05231 (Human), P08505 (Mouse), P20607 (Rat), P26892 (Bovin), P26893 (Pig), P29455 (Sheep), P41323 (Canfa), P41683 (Felca), Q95181 (Horse), Q9MZR1 (Rabit)

Gene ID

3569 (IL6), 16193 (Il6), 24498, 403985, 493687, 443406, 280826, 399500

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey), Sh (Sheep), Chk (Chicken), Bov (Bovine), Bab (Baboon), Por (Porcine)

Available hosts

Mouse, Rabbit, Goat, Rat, Chicken, Sheep

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Functional assay (FN), Paraffin Sections (P), Frozen Sections (C), Immunoprecipitation (IP), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Neutralisation (NEUT), ELISA (detection) (E(detection)), EPair for Elisa (EPair), ELISA (capture) (E(capture)), Luminex (LMNX)

Background of Interleukin-6 / IL6 antibody

IL-6 is a pleotropic cytokine that regulates the development, proliferation and maturation of a number of hematopoietic cells and is essential for the maturation of B cells into immunoglobulin-secreting cells. IL-6 can also influence the growth and function of non-hematopoietic cells including the differentiation of nerve cells, metabolism of bone and induction of acute phase proteins in hepatocytes. IL-6 is predominately expressed by T cells, macrophages, fibroblasts, endothelial cells and keratinocytes. IL-6 expression can be stimulated by a number of different factors including, T cell mitogens, LPS, viruses, IL-1, TNF, IL-2, IFN-b, platelet derived growth factor (PDGF), protein kinase C, calcium ionophore A23187 and factors that increase the intracellular concentration of cAMP. IL-6 is expressed as a glycoprotein with a variable molecular mass as a result of differential glycosylation/phosphorylation patterns. At least six distinct IL-6 phosphoglycoproteins have been identified. The lower molecular weight species, 23- to 25-kDa, are O-glycosylated while the 28- to 30-kDa species are both O- and N-glycosylated. This is a HCX protein. HCX Expression System Details HCX proteins mimic the proteins in the human body because they are expressed from human, rather than animal, insect or bacterial cells. This process gives them human post-translational modifications. Recombinant DNA techniques allow a human protein with the correct amino acid sequence to be expressed in a non-human cell line. However, non-human cells lack the appropriate cellular machinery, such as specific glycosyltransferases, necessary to produce the correct human post-translational modifications of a protein. An extreme example is seen in E. coli cells, which produce recombinant proteins with no glycosylation, as the above figure illustrates. Rodent and yeast cells are able to glycosylate proteins, but they are still different from glycosylation in human cells. Expression System Resultant Proteins Human (e.g. K562, HEK293) Correct amino acid sequence Human post-translational modifications Rodent (e.g. CHO, NSO) Correct amino acid sequence Some natural glycosylation - not human-like Yeast (e.g. Pichia) Correct amino acid sequence Some natural glycosylation - not human-like E.Coli Correct amino acid sequence No PTMs Although there have been significant attempts to make non-human cell derived cytokines more human-like, there is a growing awareness that in many instances, particularly in therapeutics, cytokines should mimic those found in the body as closely as possible.

General readings

Adam, N. et al., J. Virol.,2009; JVI.01601-08.
Ancey, C. et al. ,Cardiovasc Res.,2003; 59(1): 78-85.
Choy, E. Ann Rheum Dis.,2003; 62 Suppl 2: ii68-9.
Corbellino M. et al., Blood, 2001; 98 : 3473 - 3475
Delneste Y. et al., Blood, 2003; 101 : 143 - 150
Derenne S. et al., Blood 2002; 100 : 194 - 199
Derhaschnig, U. et al.,Crit Care Med.,2004; 32(5): 1136-40.
Dore, P. et al.,Clin Exp Immunol.,1997; 107(1): 182-8.
Foussat, A. et al.,Eur Cytokine Netw.,1999; 10(4): 501-8.
Garban, F. et al., Blood,2006; 107(9): 3474-80.
Gu, Z. J. et al., Leukemia,2000; 14(1): 188-97.
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Kricka, L. J., Clin Chem.,1999; 45(7): 942-56.
Kube, D. et al., Blood,2001; 98(3): 762-70.
Leblond, V. et al., J Clin Oncol.,2001; 19(3): 772-8.
Lelievre, E. et al.,Cytokine,1998; 10(11): 831-40.
Lu, Z. Y. et al., Blood,1995; 86(8): 3123-31.
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Park, C. S. et al.,Am J Respir Crit Care Med., 2000; 162(3 Pt 1): 1162-8.
Smith, M. D. et al., Ann Rheum Dis.,2003; 62(4): 303-7.
Song, X. Y. et al.,Mol Interv.,2002; 2(1): 36-46
Sprynski, A. C. et al., Blood,2009; blood-2008-07-170464.
Srivani, R. et al.,Current Science,2003;84(3): 434-438
Stenger, S. et al.,J Immunol.,1998; 161(7): 3582-8.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Interleukin-6 / IL6 antibody

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500065) Mouse IgG2b OTI2C9 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Interleukin-6 / IL6 antibody

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500067) Mouse IgG2a OTI3G9 Purified Hu ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

Interleukin-6 / IL6 antibody

Anti-IL-6 mouse monoclonal antibody (TA500071) immunofluorescent staining of HELA cells transiently transfected by pCMV6-ENTRY IL-6(RC202078). Mouse IgG1 OTI3H6 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Interleukin-6 / IL6 antibody

Figure 1. Formalin-Fixed, Paraffin-Embedded Sections of Human cervical squamous cell carcinoma stained with IL6 antibody Cat.-No AP01157PU at 0.25-0.5 µg/ml Rabbit Aff - Purified Hu E, FN, ICC/IF, P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

Interleukin-6 / IL6 antibody

Figure 1. Formalin-Fixed, Paraffin-Embedded Sections of Human cervical squamous cell carcinoma stained with IL6 antibody Cat.-No AP01157PU at 0.25-0.5 µg/ml Rabbit Aff - Purified Hu E, FN, ICC/IF, P, WB
50 µg / €230.00
  Acris Antibodies GmbH

Interleukin-6 / IL6 antibody

Immunohistochemistry-Paraffin: IL6 Antibody (6D9A1,6C12B1) [NBP1-47355] - Immunohistochemical analysis of paraffin-embedded lung cancer tumor using HMGB1 Rabbit pAb with DAB staining. Mouse IgG1 6D9A1,6C12B1 Purified Hu E, P, WB
0.1 ml / €380.00
  Novus Biologicals Inc.

Interleukin-6 / IL6 antibody

WB using anti-IL6 antibody. The blot shows detection of a band ~21 kDa in size corresponding to anti-IL6 antibody. Molecular weight markers are also shown (MW). After transfer, the membrane was blocked for 30 minutes with 1% BSA-TBST. Detection occurred using peroxidase conjugated anti-Rabbit IgG secondary antibody diluted 1:40,000 in blocking buffer for 30 min at RT followed by reaction with FemtoMax™ chemiluminescent substrate. Rabbit Serum Hu C, E, FN, IP, P, WB
1 ml / €900.00
  OriGene Technologies, Inc.

Interleukin-6 / IL6 antibody

Anti-mouse IL-6 antibody in WB shows detection of recombinant mouse IL-6 raised in E.coli. Recombinant truncated protein (0.1 µg, 21.7 kDa) was loaded on to an SDS-PAGE gel, and after separation, transferred to nitrocellulose. The membrane was incubated with Anti-Mouse IL-6 antibody diluted 1:1,000 in 1% BSA in TBST overnight at 4°C. After washes, the blot was reacted with secondary antibody Dylight™ 649 Conjugated Anti-Rabbit IgG (H&L) (Goat) Antibody diluted 1:20,000. Rabbit IgG Purified Ms E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-6 / IL6 antibody

To detect Human IL-6 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Human IL-6. Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Interleukin-6 / IL6 antibody

To detect Human IL-6 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Human IL-6. Goat IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Interleukin-6 / IL6 antibody

To detect Human IL-6 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-6 (TA328215) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL-6. Goat IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-6 / IL6 antibody

To detect Human IL-6 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-6 (TA328217) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL-6. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-6 / IL6 antibody

To detect Murine IL-6 by direct ELISA (using 100 ml/well) a concentration of approximately 1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody allows the detection of at least 0.2-0.4 ng/well of recombinant Murine IL-6. Rabbit IgG Purified E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Interleukin-6 / IL6 antibody

To detect Murine IL-6 by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine IL-6 (TA328488) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Murine IL-6. Rabbit IgG Purified E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-6 / IL6 antibody

To detect Rat IL-6 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat IL-6. Goat IgG Purified Rt E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Interleukin-6 / IL6 antibody

To detect Rat IL-6 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Rat IL-6 (TA328572) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-6. Goat IgG Purified Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-6 / IL6 antibody

To detect Rat IL-6 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Rat IL-6  as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-6. Rabbit IgG Purified Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-6 / IL6 antibody

Anti-IL-6 antibody IHC of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Purified Hu
50 µg / €379.00
  OriGene Technologies, Inc.

Interleukin-6 / IL6 antibody

Anti-IL-6 antibody IHC of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Purified Hu
50 µg / €379.00
  OriGene Technologies, Inc.

Interleukin-6 / IL6 antibody

IL-6 antibody (2G3) at 1:2000 + recombinant human IL-6 (Cat# RC202078) Mouse IgM OTI2G3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

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