Interleukin-6 / IL6 antibody

Principal name

Interleukin-6 / IL6 antibody

Alternative names for Interleukin-6 / IL6 antibody

IL-6, Interferon beta-2, IFNB2, B-cell stimulatory factor 2, BSF-2, CDF, Hybridoma growth factor, CTL differentiation factor

SwissProt ID

P05231 (Human), P08505 (Mouse), P20607 (Rat), P26892 (Bovin), P26893 (Pig), P29455 (Sheep), P41323 (Canfa), P41683 (Felca), Q95181 (Horse), Q9MZR1 (Rabit)

Gene ID

3569 (IL6), 16193 (Il6), 280826, 24498, 403985, 443406

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Rt (Rat), Mky (Monkey), Sh (Sheep), Chk (Chicken), Bab (Baboon)

Available hosts

Mouse, Rat, Rabbit, Goat, Sheep

Available applications

Flow Cytometry (F), Enzyme Immunoassay (E), Neutralisation (NEUT), Frozen Sections (C), Immunoprecipitation (IP), Functional assay (FN), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (capture) (E(capture)), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), EPair for Elisa (EPair), Luminex (LMNX)

Background of Interleukin-6 / IL6 antibody

Interleukin 6(IL-6) is a multifunctional protein produced by lymphoid and non-lymphoid cells and by normal and transformed cells, including T cells, monocyte/macrophages, fibroblasts, hepatocytes, vascular endothelial cells, cardiac myxomas, bladder cell carcinomas, myelomas, astrogliomas and glioblastomas. The production of IL-6 in these cells is regulated, either positively or negatively, by a variety of signals including mitogens, antigenic stimulation, lipopolysaccharides, IL-1, TNF, PDGF and viruses. For reviews on IL-6, see references 1-5.

The human IL-6 cDNA sequence predicts a protein of 212 amino acid (aa) residues in length with two potential N-glycosylation sites. The hydrophobic N-terminal 28 aa residue signal peptide is cleaved to produce a mature protein of 184 amino acids with four cysteine residues and a predicted molecular mass of 21 kDa . The mouse IL-6 cDNA sequence shows a homology of 42% at the aa level when compared with the human sequence . On the basis of sequence similarity and gene structural motif similarity, IL-6 can be grouped in a family of cytokines that also includes OSM, G-CSF, LIF, and CNTF. All of these cytokines are predicted to have a four helix bundle structure similar to that found for growth hormone, suggesting that they all evolved from a common ancestral gene .

The effects of IL-6 on different cells are numerous and varied. The effect on B cells is stimulation of differentiation and antibody secretion . IL-6 also affects T cells, acting as a co-stimulant with sub-optimal concentrations of PHA or Con A to stimulate IL-2 production and IL-2 receptor expression.

IL-6 exhibits growth factor activity for mature thymic or peripheral T-cells and reportedly enhances the differentiation of cytotoxic T-cells in the presence of IL-2 or IFN-. . IL-6 stimulates production of acute phase proteins by hepatocytes and has colony-stimulating activity on hematopoietic stem cells. IL-6 has growth factor activities and will stimulate the growth of myeloma/hybridoma/ plasmacytoma cells, EBV-transformed B cells, keratinocytes and mesangial cells .

General readings

Adam, N. et al., J. Virol.,2009; JVI.01601-08.
Ancey, C. et al. ,Cardiovasc Res.,2003; 59(1): 78-85.
Choy, E. Ann Rheum Dis.,2003; 62 Suppl 2: ii68-9.
Corbellino M. et al., Blood, 2001; 98 : 3473 - 3475
Delneste Y. et al., Blood, 2003; 101 : 143 - 150
Derenne S. et al., Blood 2002; 100 : 194 - 199
Derhaschnig, U. et al.,Crit Care Med.,2004; 32(5): 1136-40.
Dore, P. et al.,Clin Exp Immunol.,1997; 107(1): 182-8.
Foussat, A. et al.,Eur Cytokine Netw.,1999; 10(4): 501-8.
Garban, F. et al., Blood,2006; 107(9): 3474-80.
Gu, Z. J. et al., Leukemia,2000; 14(1): 188-97.
Haddad E. et al., Blood, 2001; 97 : 1590 - 1597
Jego G. et al., Blood, 2001; 97(6) : 1817 - 1822
Jourdan, M. et al.,Br J Haematol.,1998; 100(4): 637-46.
Kalai, M. et al.,Blood,1997; 89(4): 1319-33.
Kricka, L. J., Clin Chem.,1999; 45(7): 942-56.
Kube, D. et al., Blood,2001; 98(3): 762-70.
Leblond, V. et al., J Clin Oncol.,2001; 19(3): 772-8.
Lelievre, E. et al.,Cytokine,1998; 10(11): 831-40.
Lu, Z. Y. et al., Blood,1995; 86(8): 3123-31.
Lu, Z. Y. et al., Blood,1995; 85(9): 2521-7.
Mahtouk K. et al., Blood, 2004; 103(5): 1829
Marfaing-Koka, A. et al., J Acquir Immune Defic. Syndr. Hum. Retrovirol.,1996; 11(1): 59-68.
Menetrier-Caux, C. et al., Cancer Res.,2001; 61(7): 3096-104.
Moreau P. et al., Blood, 2006; 107 (1) :397-403
Moreaux J. et al., Blood, 2004; 103(8) : 3148 - 3157
Park, C. S. et al.,Am J Respir Crit Care Med., 2000; 162(3 Pt 1): 1162-8.
Smith, M. D. et al., Ann Rheum Dis.,2003; 62(4): 303-7.
Song, X. Y. et al.,Mol Interv.,2002; 2(1): 36-46
Sprynski, A. C. et al., Blood,2009; blood-2008-07-170464.
Srivani, R. et al.,Current Science,2003;84(3): 434-438
Stenger, S. et al.,J Immunol.,1998; 161(7): 3582-8.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Interleukin-6 / IL6 antibody

  IL6 mouse monoclonal antibody, clone OTI2C9 (formerly 2C9)  
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500065) Mouse IgG2b OTI2C9 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Interleukin-6 / IL6 antibody

  IL6 mouse monoclonal antibody, clone OTI3G9 (formerly 3G9)  
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500067) Mouse IgG2a OTI3G9 Purified Hu ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

Interleukin-6 / IL6 antibody

  IL6 mouse monoclonal antibody, clone OTI3H6 (formerly 3H6)  
Anti-IL-6 mouse monoclonal antibody (TA500071) immunofluorescent staining of HELA cells transiently transfected by pCMV6-ENTRY IL-6(RC202078). Mouse IgG1 OTI3H6 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Interleukin-6 / IL6 antibody

Figure 1. Formalin-Fixed, Paraffin-Embedded Sections of Human cervical squamous cell carcinoma stained with IL6 antibody Cat.-No AP01157PU at 0.25-0.5 µg/ml Rabbit Aff - Purified Hu E, FN, ICC/IF, P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

Interleukin-6 / IL6 antibody

Figure 1. Formalin-Fixed, Paraffin-Embedded Sections of Human cervical squamous cell carcinoma stained with IL6 antibody Cat.-No AP01157PU at 0.25-0.5 µg/ml Rabbit Aff - Purified Hu E, FN, ICC/IF, P, WB
50 µg / €230.00
  Acris Antibodies GmbH

Interleukin-6 / IL6 antibody

  Rabbit polyclonal anti-IL-6 antibody  
WB using anti-IL6 antibody. The blot shows detection of a band ~21 kDa in size corresponding to anti-IL6 antibody. Molecular weight markers are also shown (MW). After transfer, the membrane was blocked for 30 minutes with 1% BSA-TBST. Detection occurred using peroxidase conjugated anti-Rabbit IgG secondary antibody diluted 1:40,000 in blocking buffer for 30 min at RT followed by reaction with FemtoMax™ chemiluminescent substrate. Rabbit Serum Hu C, E, FN, IP, P, WB
1 ml / €1,070.00
  OriGene Technologies, Inc.

Interleukin-6 / IL6 antibody

  Rabbit polyclonal anti-IL6 antibody  
Anti-mouse IL-6 antibody in WB shows detection of recombinant mouse IL-6 raised in E.coli. Recombinant truncated protein (0.1 µg, 21.7 kDa) was loaded on to an SDS-PAGE gel, and after separation, transferred to nitrocellulose. The membrane was incubated with Anti-Mouse IL-6 antibody diluted 1:1,000 in 1% BSA in TBST overnight at 4°C. After washes, the blot was reacted with secondary antibody Dylight™ 649 Conjugated Anti-Rabbit IgG (H&L) (Goat) Antibody diluted 1:20,000. Rabbit IgG Purified Ms E, P, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

Interleukin-6 / IL6 antibody

  Biotinylated Anti-Human IL-6 Rabbit Polyclonal Antibody  
To detect Human IL-6 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Human IL-6. Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Interleukin-6 / IL6 antibody

  Biotinylated Anti-Human IL-6 Goat Polyclonal Antibody  
To detect Human IL-6 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Human IL-6. Goat IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Interleukin-6 / IL6 antibody

  Anti-Human IL-6 Goat Polyclonal Antibody  
To detect Human IL-6 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-6 (TA328215) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL-6. Goat IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-6 / IL6 antibody

  Anti-Human IL-6 Rabbit Polyclonal Antibody  
To detect Human IL-6 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-6 (TA328217) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL-6. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-6 / IL6 antibody

  Biotinylated Anti-Murine IL-6 Rabbit Polyclonal Antibody  
To detect Murine IL-6 by direct ELISA (using 100 ml/well) a concentration of approximately 1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody allows the detection of at least 0.2-0.4 ng/well of recombinant Murine IL-6. Rabbit IgG Purified E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Interleukin-6 / IL6 antibody

  Anti-Murine IL-6 Rabbit Polyclonal Antibody  
To detect Murine IL-6 by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine IL-6 (TA328488) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Murine IL-6. Rabbit IgG Purified E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-6 / IL6 antibody

  Biotinylated Anti-Rat IL-6 Goat Polyclonal Antibody  
To detect Rat IL-6 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat IL-6. Goat IgG Purified Rt E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Interleukin-6 / IL6 antibody

  Anti-Rat IL-6 Goat Polyclonal Antibody  
To detect Rat IL-6 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Rat IL-6 (TA328572) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-6. Goat IgG Purified Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-6 / IL6 antibody

  Anti-Rat IL-6 Rabbit Polyclonal Antibody  
To detect Rat IL-6 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Rat IL-6  as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-6. Rabbit IgG Purified Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-6 / IL6 antibody

  Rabbit Polyclonal Anti-IL6 Antibody (Internal)  
Anti-IL-6 antibody IHC of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Purified Hu
50 µg / €400.00
  OriGene Technologies, Inc.

Interleukin-6 / IL6 antibody

  Rabbit Polyclonal Anti-IL6 Antibody (Internal)  
Anti-IL-6 antibody IHC of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Purified Hu
50 µg / €400.00
  OriGene Technologies, Inc.

Interleukin-6 / IL6 antibody

  Rat Monoclonal Anti-IL-6 Antibody  
Interleukin-6 / IL6 Rat IgG1 MP5-20F3 Purified Ms E, WB
0.5 mg / €413.00
  OriGene Technologies, Inc.

Interleukin-6 / IL6 antibody

  anti-IL6 mouse monoclonal antibody, clone OTI2G3 (formerly 2G3)  
IL-6 antibody (2G3) at 1:2000 + recombinant human IL-6 (Cat# RC202078) Mouse IgM OTI2G3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

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