Interleukin-7 / IL7 antibody

Principal name

Interleukin-7 / IL7 antibody

Alternative names for Interleukin-7 / IL7 antibody

IL-7

SwissProt ID

P10168 (Mouse), P13232 (Human), P26895 (Bovin), P56478 (Rat), Q28540 (Sheep), Q9N2G6 (Pig)

Gene ID

16196 (Il7), 3574 (IL7), 25647

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit, Rat, Sheep

Available applications

Enzyme Immunoassay (E), Functional assay (FN), Western blot / Immunoblot (WB), Neutralisation (NEUT), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Immunoprecipitation (IP), ELISA (capture) (E(capture)), Flow Cytometry (F), Frozen Sections (C)

Background of Interleukin-7 / IL7 antibody

Interleukin 7 (IL7) is a lymphoid cell growth factor that affects pre-B, pro-B, and early T cells. IL7 was previously known as pre-B cell growth factor and lymphopoietin 1. IL7 supports the growth of early B cells from long-term lymphoid bone marrow cultures. It is mitogenic to thymocytes and enhances the response of cells to other stimuli such as polyhydroxyalkanoate (PHA) and concanavalin A (ConA). IL7 stimulates the proliferation of CD4+/CD8+ cells. The proliferative response of thymocytes to IL7 is not affected by antibodies to the T cell growth factors such as IL2, IL4 and IL6, suggesting that IL7 is capable of stimulating T cell proliferation through a pathway independent of the known T cell growth factors. Mature T cells respond to IL7 and Con A, but not to IL7 alone. In myeloid lineage cells, IL7 upregulates the production of pro-inflammatory cytokines and stimulates the tumoricidal activity of monocytes/macrophages. IL7 is expressed by adherent stromal cells from various tissues.

General readings

1. Sutherland, G. R., Baker, E., Fernandez, K. E. W., Callen, D. F., Goodwin, R. G., Lupton, S., Namen, A. E., Shannon, M. F., Vadas, M. A. The gene for human interleukin 7 (IL7) is at 8q12-13. Hum. Genet. 82: 371-372, 1989.
2. Liu, X., Leung, S., Wang, C., Tan, Z., Wang, J., Guo, T. B., Fang, L., Zhao, Y., Wan, B., Qin, X., Lu, L., Li, R., Pan, H.,
Song, M., Liu, A., Hong, J., Lu, H., Zhang, J. Z. Crucial role of interleukin-7 in T helper type 17 survival and expansion in
autoimmune disease. Nature Med. 16: 191-197, 2010. [PubMed: 20062065

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Interleukin-7 / IL7 antibody

Western Blot (Reduced) using IL7 Antibody Cat.-No PP013B Rabbit Biotin Ms E, WB
25 µg / €230.00
  Acris Antibodies GmbH

Interleukin-7 / IL7 antibody

Western Blot (Reduced) using IL7 Antibody Cat.-No PP013B Rabbit Biotin Ms E, WB
50 µg / €340.00
  Acris Antibodies GmbH

Interleukin-7 / IL7 antibody

Western blot analysis using recombinant rat IL-7. Lane 1: 50 ng, Lane 2: 100 ng, Lane 3: 200 ng, Lane 4: 500 ng. Rabbit Purified Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-7 / IL7 antibody

anti-Human IL-7 antibody shows detection of a band ~17 kDa in size corresponding to recombinant human IL-7. The identity of the faint higher molecular weight band may represent a homodimer. Molecular weight markers are also shown (left). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution. Detection occurred using peroxidase conjugated anti-Rabbit IgG (p/n 611-103-122) secondary antibody diluted 1:40,000 Rabbit IgG Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-7 / IL7 antibody

anti-Human IL-7 antibody shows detection of a band ~17 kDa in size corresponding to recombinant human IL-7. The identity of the faint higher molecular weight band may represent a homodimer. Molecular weight markers are also shown (left). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution. Detection occurred using peroxidase conjugated anti-Rabbit IgG (p/n 611-103-122) secondary antibody diluted 1:40,000. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-7 / IL7 antibody

WB showing detection of Human IL-7. 50ng of Human IL-7 (Lane 1) was run on a 4-20% gel and transferred to 0.45µm nitrocellulose. After blocking with 5% Blotto (p/n B501-0500) 30 min at 20°C, Anti-Human IL-7 (RABBIT) Antibody Biotin Conjugated (p/n TA319317) secondary antibody was used at 1:1000. HRP Streptavidin (p/n S000-03) was used at 1:40,000. Arrow indicates correct 17 kDa molecular weight position expected for Human IL-7. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-7 / IL7 antibody

Western blot analysis of extracts of various cell lines, using IL7 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-7 / IL7 antibody

To detect Human IL-7 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Human IL-7. Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Interleukin-7 / IL7 antibody

To detect Human IL-7 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-7 (TA328218) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL-7. Rabbit IgG Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-7 / IL7 antibody

To detect Murine IL-7 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Murine IL-7. Rabbit IgG Purified E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Interleukin-7 / IL7 antibody

To detect Murine IL-7 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine IL-7 (TA328490) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Murine IL-7. Rabbit IgG Purified E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-7 / IL7 antibody

ELISA: IL7 Antibody Rabbit IgG Purified Hu E
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-7 / IL7 antibody

Interleukin-7 / IL7 Rat IgG1 BVD10-40F6 Purified Hu E
0.5 mg / €440.00
  Acris Antibodies GmbH

Interleukin-7 / IL7 antibody

Interleukin-7 / IL7 Rat IgG2a BVD10-11C10 Biotin Hu E
0.5 mg / €540.00
  Acris Antibodies GmbH

Interleukin-7 / IL7 antibody

Interleukin-7 / IL7 Mouse IgG1 B-S16 Azide Free Hu
0.5 mg / €460.00
  Acris Antibodies GmbH

Interleukin-7 / IL7 antibody

Interleukin-7 / IL7 Mouse IgG1 B-N18 Azide Free Hu E(capture)
1 mg / €790.00
  Acris Antibodies GmbH

Interleukin-7 / IL7 antibody

Interleukin-7 / IL7 Mouse IgG1 B-S16 Biotin Hu E(detection)
0.1 mg / €460.00
  Acris Antibodies GmbH

Interleukin-7 / IL7 antibody

Western Blot (Reduced) using IL7 antibody Cat.-No AP32852BT Rabbit Biotin Rt E, WB
50 µg / €340.00
  Acris Antibodies GmbH

Interleukin-7 / IL7 antibody

Western Blot (Reduced) using IL7 antibody Cat.-No AP32852BT Rabbit Biotin Rt E, WB
25 µg / €230.00
  Acris Antibodies GmbH

Interleukin-7 / IL7 antibody

Western Blot (Unreduced) using IL7 antibody Cat.-No AP32852PU Rabbit Aff - Purified Rt E, NEUT, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

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