Interleukin-8 / IL8 antibody

Principal name

Interleukin-8 / IL8 antibody

Alternative names for Interleukin-8 / IL8 antibody

CXCL8, Protein 3-10C, Emoctakin, GCP1, MDNCF, MONAP, NAP1, Monocyte-derived neutrophil chemotactic factor, T-cell chemotactic factor, Neutrophil-activating protein 1, Granulocyte chemotactic protein 1, Monocyte-derived neutrophil-activating peptide

SwissProt ID

O62812 (Horse), P08317 (Chick), P10145 (Human), P19874 (Rabit), P26894 (Pig), P36925 (Sheep), P41324 (Canfa), P49113 (Cavpo), P79255 (Bovin), Q9XSX5 (Felca)

Gene ID

3576 (IL8), 1003576 (gnd), 280828, 396880, 100037400, 443418

Available reactivities

Hu (Human), Sh (Sheep), Por (Porcine), Mky (Monkey), Bov (Bovine), Ms (Mouse), Rb (Rabbit)

Available hosts

Mouse, Rabbit, Chicken, Goat, Sheep

Available applications

Enzyme Immunoassay (E), Functional assay (FN), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Flow Cytometry (F), Paraffin Sections (P), ELISA (capture) (E(capture)), Neutralisation (NEUT), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Luminex (LMNX)

Background of Interleukin-8 / IL8 antibody

Interleukin-8 selectively stimulates the ability of neutrophils and T-lymphocytes to invade injured or inflamed tissue. IL-8 also known as CXCL8 that is purified from peripheral blood monocytes is a 10 kDa protein showing at least 4 variant forms (77, 72, 70, and 69 amino acids, respectively) which differ in the length of the N-terminal and readily form dimers in solution. Two cystin-bridges are essential for the biological activity of IL-8. Exogenous stimuli like LPS, but also IL-1, TNF-alpha and TNF-beta induce the secretion of IL-8 in a variety of different cell types including monocytes, endothelial and epithelial cells, peripheral blood mononuclear cells, dermal fibroblasts, keratinocytes, neutrophils, hepatocytes, synovial cells, and T-lymphocytes. The activities of IL-8 are not species-specific. Human IL-8 is also active in rodent and rabbit cells and differs from all other cytokines in its ability to specifically activate neutrophil granulocytes. This chemokine activates multiple intracellular signaling pathways downstream of two cell-surface, G protein-coupled receptors (CXCR1 and CXCR2). The property of IL-8 to stimulate movement of neutrophils across endothelial monolayers in vitro supports the concept of a central role for this molecule in the accumulation of neutrophils at inflammatory lesions in vivo.

General readings

1、Wolff B, Burns AR, Middleton J, Rot A (November 1998). "Endothelial cell "memory" of inflammatory stimulation: human venular endothelial cells store interleukin 8 in Weibel-Palade bodies".
2、Utgaard JO, Jahnsen FL, Bakka A, Brandtzaeg P, Haraldsen G (November 1998). "Rapid secretion of prestored interleukin 8 from Weibel-Palade bodies of microvascular endothelial cells".
3、 Modi WS, Dean M, Seuanez HN, Mukaida N, Matsushima K, O'Brien SJ (January 1990). "Monocyte-derived neutrophil chemotactic factor (MDNCF/IL-8) resides in a gene cluster along with several other members of the platelet factor 4 gene superfamily".
4、"Entrez Gene: IL8 interleukin 8".
5、 Köhidai L, Csaba G (July 1998). "Chemotaxis and chemotactic selection induced with cytokines (IL-8, RANTES and TNF-alpha) in the unicellular Tetrahymena pyriformis".
6、O'Donovan, N., Galvin, M., Morgan, J. G. Physical mapping of the CXC chemokine locus on human chromosome 4. Cytogenet.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Interleukin-8 / IL8 antibody

Immunoblot of human peripheral blood mononuclear cells either left untreated or treated with LPS and Brefeldin A for 4 hours. Lane 1, non-reduced lysate prepared from stimulated cells; lane 2, non-reduced lysate of unstimulated cells; lane 3, reduced lysate of stimulated cells; lane 4, reduced lysate prepared from unstimulated cells. Bands were visualized using Anti-Mouse IgG HRP. Mouse N11 Purified Hu WB
25 µg / €150.00
  OriGene Technologies, Inc.

Interleukin-8 / IL8 (C-term) antibody

Western blot analysis of IL8 Antibody (C-term) (Cat. #TA325052) in MDA-MB468 cell line lysates (35ug/lane). IL8 (arrow) was detected using the purified Pab. Rabbit Ig Purified Hu F, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

Interleukin-8 / IL8 antibody

To detect Human IL-8 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Human IL-8. Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Interleukin-8 / IL8 antibody

To detect Human IL-8 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-8 (TA328220) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL-8. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-8 / IL8 antibody

Western blot detection of IL8 in CHO-K1 transfected by IL8-PDGFR fusion protein cell lysate using IL8 mouse mAb (1:500(A)-1:1000(B) diluted). Mouse IgG2b 6E7-C11-C9 Purified WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Interleukin-8 / IL8 antibody

Western blot analysis of Interleukin 8 expression in HeLa cells, Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-8 / IL8 antibody

IL-8 ELISA with SNAP8 Capture (TA600024) and 8CH Detection (TA700024) Antibodies. Substrate used: Recombinant Human IL-8 (E. Coli expressed Peptide) Mouse IgG1 OTISP8 Purified Hu E, LMNX
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-8 / IL8 antibody

IL-8 ELISA with SNAP8 Capture (TA600024) and 8CH Detection (TA700024) Antibodies. Substrate used: Recombinant Human IL-8 (E. Coli expressed Peptide) Mouse IgG1 OTI8CH Purified Hu E, LMNX
50 µg / €345.00
  OriGene Technologies, Inc.

Interleukin-8 / IL8 antibody

Interleukin-8 / IL8 Mouse IgG1 BDI670 Azide Free Hu E, FN, WB
0.5 mg / €710.00
  Acris Antibodies GmbH

Interleukin-8 / IL8 antibody

Interleukin-8 / IL8 Mouse IgG1 AS14 Purified Hu E, IP
0.5 mg / €400.00
  Acris Antibodies GmbH

Interleukin-8 / IL8 antibody

Interleukin-8 / IL8 Mouse IgG1 AS15 Biotin Hu E
0.2 mg / €400.00
  Acris Antibodies GmbH

Interleukin-8 / IL8 antibody

Interleukin-8 / IL8 Mouse IgG1 AS13 FITC Hu F
100 Tests / €360.00
  Acris Antibodies GmbH

Interleukin-8 / IL8 antibody

Interleukin-8 / IL8 Mouse IgG1 NAPII FITC Hu
100 Tests / €360.00
  Acris Antibodies GmbH

Interleukin-8 / IL8 antibody

Western blot analysis using IL8 antibody Cat.-No AM06083PU-N against IL8 recombinant protein. Mouse IgG1 3E4D8 Purified Hu E, WB
0.1 ml / €300.00
  Acris Antibodies GmbH

Interleukin-8 / IL8 antibody

Western blot analysis of Human IL-8 using anti-human IL8 Antibody Mouse IgG1 I8-61 Azide Free E, FN, WB
0.5 mg / €470.00
  Acris Antibodies GmbH

Interleukin-8 / IL8 antibody

Western blot analysis of Human IL-8 using anti-human IL8 Antibody Mouse IgG1 257 Azide Free E, FN, WB
0.5 mg / €470.00
  Acris Antibodies GmbH

Interleukin-8 / IL8 antibody

Western blot analysis of Human IL-8 using anti-human IL8 Antibody Mouse IgG1 807 Azide Free E, FN, WB
0.5 mg / €470.00
  Acris Antibodies GmbH

Interleukin-8 / IL8 antibody

Interleukin-8 / IL8 Mouse IgG1 B-K8 Azide Free Hu F, FN
0.5 mg / €460.00
  Acris Antibodies GmbH

Interleukin-8 / IL8 antibody

Interleukin-8 / IL8 Mouse IgG1 B-K8 PE Hu F
1ml (100T) / €340.00
  Acris Antibodies GmbH

Interleukin-8 / IL8 antibody

Interleukin-8 / IL8 Rabbit IgG Serum Hu E
1 ml / €1,150.00
  Acris Antibodies GmbH

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