Interleukin-8 / IL8 antibody

Principal name

Interleukin-8 / IL8 antibody

Alternative names for Interleukin-8 / IL8 antibody

CXCL8, Protein 3-10C, Emoctakin, GCP1, MDNCF, MONAP, NAP1, Monocyte-derived neutrophil chemotactic factor, T-cell chemotactic factor, Neutrophil-activating protein 1, Granulocyte chemotactic protein 1, Monocyte-derived neutrophil-activating peptide

SwissProt ID

O62812 (Horse), P08317 (Chick), P10145 (Human), P19874 (Rabit), P26894 (Pig), P36925 (Sheep), P41324 (Canfa), P49113 (Cavpo), P79255 (Bovin), Q9XSX5 (Felca)

Gene ID

3576 (IL8), 100037400, 280828, 100620730 (LOC100620730), 443418

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ft (Ferret), Mink, Por (Porcine), Rb (Rabbit), Sh (Sheep), Md (Mustelid), Mky (Monkey)

Available hosts

Goat, Mouse, Rabbit, Sheep

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Functional assay (FN), Paraffin Sections (P), Flow Cytometry (F), ELISA (capture) (E(capture)), Neutralisation (NEUT), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Luminex (LMNX)

Background of Interleukin-8 / IL8 antibody

Interleukin 8 (IL-8), a member of the neutrophil-specific CXC subfamily of chemokines, is a potent neutrophil chemotactic and activating factor. It is a primary inflammatory cytokine produced by many cells including monocytes/ macrophages, T cells, neutrophils, fibroblasts, endothelial cells, keratinocytes, hepatocytes, astrocytes and chondrocytes. It is in response to proinflammatory stimuli such as IL-1, TNF, LPS and viruses. Its function is, in part, to attract neutrophils to the site of inflammation and to activate them.

The human IL-8 cDNA sequence predicts a protein of 99 amino acids. Removal of a 22-residue signal peptide generates a mature protein of 77 amino acids (~ 8 kDa). Further proteolysis of the N-terminal end leads to a variant form with 72 amino acids; full activation of IL-8 may require cleavage to the 72 amino acid form.

IL-8 can form non-covalent dimers in solution, especially at high concentrations, but dimerization is not necessary for biological activity. IL-8 binds to two seven-transmembrane, G protein-coupled receptors, CXCR1 and CXCR2, as well as to the non-signalling Duffy antigen on red-blood cells. The Duffy antigen may play a role in regulating IL-8 activity on functional receptors.

General readings

1、Wolff B, Burns AR, Middleton J, Rot A (November 1998). "Endothelial cell "memory" of inflammatory stimulation: human venular endothelial cells store interleukin 8 in Weibel-Palade bodies".
2、Utgaard JO, Jahnsen FL, Bakka A, Brandtzaeg P, Haraldsen G (November 1998). "Rapid secretion of prestored interleukin 8 from Weibel-Palade bodies of microvascular endothelial cells".
3、 Modi WS, Dean M, Seuanez HN, Mukaida N, Matsushima K, O'Brien SJ (January 1990). "Monocyte-derived neutrophil chemotactic factor (MDNCF/IL-8) resides in a gene cluster along with several other members of the platelet factor 4 gene superfamily".
4、"Entrez Gene: IL8 interleukin 8".
5、 Köhidai L, Csaba G (July 1998). "Chemotaxis and chemotactic selection induced with cytokines (IL-8, RANTES and TNF-alpha) in the unicellular Tetrahymena pyriformis".
6、O'Donovan, N., Galvin, M., Morgan, J. G. Physical mapping of the CXC chemokine locus on human chromosome 4. Cytogenet.

Items 1 to 20 of 63 total

per page
Page:
  1. 1
  2. 2
  3. 3
  4. 4

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Interleukin-8 / IL8 antibody

  Mouse Anti-Human IL-8 (1-77) (CXCL8) Purified (25 ug)  
Immunoblot of human peripheral blood mononuclear cells either left untreated or treated with LPS and Brefeldin A for 4 hours. Lane 1, non-reduced lysate prepared from stimulated cells; lane 2, non-reduced lysate of unstimulated cells; lane 3, reduced lysate of stimulated cells; lane 4, reduced lysate prepared from unstimulated cells. Bands were visualized using Anti-Mouse IgG HRP. Mouse N11 Purified Hu WB
25 µg / €150.00
  OriGene Technologies, Inc.

Interleukin-8 / IL8 (C-term) antibody

  Rabbit polyclonal IL8 Antibody (C-term)  
Western blot analysis of IL8 Antibody (C-term) (Cat. #TA325052) in MDA-MB468 cell line lysates (35ug/lane). IL8 (arrow) was detected using the purified Pab. Rabbit Ig Purified Hu F, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

Interleukin-8 / IL8 antibody

  Biotinylated Anti-Human IL-8 Rabbit Polyclonal Antibody  
To detect Human IL-8 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Human IL-8. Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Interleukin-8 / IL8 antibody

  Anti-Human IL-8 Rabbit Polyclonal Antibody  
To detect Human IL-8 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-8 (TA328220) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL-8. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-8 / IL8 antibody

  Mouse Monoclonal IL8 Antibody  
Western blot detection of IL8 in CHO-K1 transfected by IL8-PDGFR fusion protein cell lysate using IL8 mouse mAb (1:500(A)-1:1000(B) diluted). Mouse IgG2b 6E7-C11-C9 Purified WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Interleukin-8 / IL8 antibody

  Rabbit Polyclonal Anti-Interleukin 8 Antibody  
Western blot analysis of Interleukin 8 expression in HeLa cells, Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-8 / IL8 antibody

  ELISA and Luminex validated IL-8 Mouse monoclonal antibody clone (SNA, clone OTISNAP8 (formerly SNAP8)  
IL-8 ELISA with SNAP8 Capture (TA600024) and 8CH Detection (TA700024) Antibodies. Substrate used: Recombinant Human IL-8 (E. Coli expressed Peptide) Mouse IgG1 OTISP8 Purified Hu E, LMNX
0.1 mg / €325.00
  OriGene Technologies, Inc.

Interleukin-8 / IL8 antibody

  ELISA and Luminex validated biotinylated IL-8 Mouse monoclonal antibody clone (8CH), clone OTI8CH (formerly 8CH)  
IL-8 ELISA with SNAP8 Capture (TA600024) and 8CH Detection (TA700024) Antibodies. Substrate used: Recombinant Human IL-8 (E. Coli expressed Peptide) Mouse IgG1 OTI8CH Purified Hu E, LMNX
50 µg / €345.00
  OriGene Technologies, Inc.

Interleukin-8 / IL8 antibody

Interleukin-8 / IL8 Mouse IgG1 BDI670 Azide Free Hu E, FN, WB
0.5 mg / €790.00
  Acris Antibodies GmbH

Interleukin-8 / IL8 antibody

Interleukin-8 / IL8 Mouse IgG1 AS14 Purified Hu E, IP
0.5 mg / €410.00
  Acris Antibodies GmbH

Interleukin-8 / IL8 antibody

Interleukin-8 / IL8 Mouse IgG1 AS15 Biotin Hu E
0.2 mg / €410.00
  Acris Antibodies GmbH

Interleukin-8 / IL8 antibody

Interleukin-8 / IL8 Mouse IgG1 AS13 FITC Hu F
100 Tests / €370.00
  Acris Antibodies GmbH

Interleukin-8 / IL8 antibody

Interleukin-8 / IL8 Mouse IgG1 NAPII FITC Hu
100 Tests / €360.00
  Acris Antibodies GmbH

Interleukin-8 / IL8 antibody

Western blot analysis using IL8 antibody Cat.-No AM06083PU-N against IL8 recombinant protein. Mouse IgG1 3E4D8 Purified Hu E, WB
0.1 ml / €300.00
  Acris Antibodies GmbH

Interleukin-8 / IL8 antibody

Western blot analysis of Human IL-8 using anti-human IL8 Antibody Mouse IgG1 I8-61 Azide Free E, FN, WB
0.5 mg / €460.00
  Acris Antibodies GmbH

Interleukin-8 / IL8 antibody

Western blot analysis of Human IL-8 using anti-human IL8 Antibody Mouse IgG1 257 Azide Free E, FN, WB
0.5 mg / €460.00
  Acris Antibodies GmbH

Interleukin-8 / IL8 antibody

Western blot analysis of Human IL-8 using anti-human IL8 Antibody Mouse IgG1 807 Azide Free E, FN, WB
0.5 mg / €460.00
  Acris Antibodies GmbH

Interleukin-8 / IL8 antibody

Interleukin-8 / IL8 Mouse IgG1 B-K8 Azide Free Hu F, FN
0.5 mg / €460.00
  Acris Antibodies GmbH

Interleukin-8 / IL8 antibody

Interleukin-8 / IL8 Mouse IgG1 B-K8 PE Hu F
1ml (100T) / €340.00
  Acris Antibodies GmbH

Interleukin-8 / IL8 antibody

Interleukin-8 / IL8 Rabbit IgG Serum Hu E
1 ml / €1,180.00
  Acris Antibodies GmbH

Items 1 to 20 of 63 total

per page
Page:
  1. 1
  2. 2
  3. 3
  4. 4
  • LinkedIn