INTS9 / RC74 antibody

Principal name

INTS9 / RC74 antibody

Alternative names for INTS9 / RC74 antibody

Int9, RC-74, Integrator complex subunit 9, Protein related to CPSF subunits of 74 kDa

SwissProt ID

Q2KJA6 (Bovin), Q5ZKK2 (Chick), Q6DFF4 (Xenla), Q8K114 (Mouse), Q9NV88 (Human)

Gene ID

55756 (INTS9)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Rt (Rat)

Available hosts

Rabbit, Mouse

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), ELISA (detection) (E(detection))

Background of INTS9 / RC74 antibody

Formation of the mature 3' ends of the vast majority of cellular mRNAs occurs through cleavage and polyadenylation and requires a cleavage and polyadenylation specificity factor (CPSF) containing, among other proteins, CPSF-73 and CPSF-100. RC-74 is an exclusively nuclear protein found in HeLa cells that has similar characteristics to CPSF-100. Like the complex that forms between CPSF-100 and CPSF-73, studies show that RC-74 may form a similar complex with RC-68 that is independent from the CPSF complex. This RC-68 / RC-74 complex may be involved in a pre-mRNA processing event other than cleavage and polyadenylation, such as the 3' end processing of a distinct subset of cellular pre-mRNAs encoding proteins required for G(1) progression and entry into S phase.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

INTS9 / RC74 antibody

Host: Rabbit; Target Name: INTS9; Sample Tissue: Fetal Lung lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

INTS9 / RC74 antibody

Western Blot analysis of INTS9 expression in transfected 293T cell line (H00055756-T01) by INTS9 MaxPab polyclonal antibody.

Lane 1: RC74 transfected lysate(72.38 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

INTS9 / RC74 antibody

Western Blot: RC74 Antibody [NB100-589]-Nuclear extract (25 mcg for WB in A; 5 mg for IP in B) from HeLa cells.   Affinity purified rabbit anti-RC74 antibody used at 0.05 mcg/ml for WB in A and at 4 mcg/5 mg extract for IP in B.  RC74 was also immunoprecipitated using rabbit anti-RC74 antibody NB100-590 at 4 mcg/5 mg extract. Detection: Chemiluminescence with an exposure time of 5 minutes in A. Coomassie stain in B.  The identity of the bands that are RC74 was verified by mass spectrometry. Rabbit Aff - Purified Hu IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

INTS9 / RC74 antibody

Western Blot: RC74 Antibody [NB100-590]-Nuclear extract (5 mg) from HeLa cells.   Affinity purified rabbit anti-RC74 at 4 mcg/5 mg extract.  RC74 was also immunoprecipitated using rabbit anti-RC74 antibody NB100-589 at 4 mcg/5 mg extract. Detection: Coomassie stain.  The identity of the bands that are RC74 was verified by mass spectrometry. Rabbit Aff - Purified Hu IP
0.1 ml / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

INTS9 / RC74 (transcript variant 1)

INTS9 / RC74 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

INTS9 / RC74

INTS9 / RC74 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

INTS9 / RC74

INTS9 / RC74 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

INTS9 overexpression lysate

INTS9 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

RC74 293T Cell Transient Overexpression Lysate(Denatured)

RC74 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

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