IRAK2 antibody

Principal name

IRAK2 antibody

Alternative names for IRAK2 antibody

IRAK-2, Interleukin-1 receptor-associated kinase-like 2

SwissProt ID

O43187 (Human), Q0P5I2 (Bovin), Q4QQS0 (Rat), Q5R810 (Ponab), Q8CFA1 (Mouse)

Gene ID

3656 (IRAK2), 108960 (Irak2)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), Paraffin Sections (P)

Background of IRAK2 antibody

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.The tyrosine-like kinase (TLK) group consists of 40 tyrosine and serine-threonine kinases such as MLK (mixed-lineage kinase), LISK (LIMK/TESK), IRAK (interleukin-1 receptor-associated kinase), Raf, RIPK (receptor-interacting protein kinase), and STRK (activin and TGF-beta receptors) families.

General readings

1. Muzio M, Ni J, Feng P, Dixit VM. IRAK (Pelle) family member IRAK-2 and MyD88 as proximal mediators of IL-1 signaling. Science 1997;278:1612-5.
2. Zhang FX, Kirschning CJ, Mancinelli R, Xu XP, Jin Y, Faure E, Mantovani A, Rothe M, Muzio M, Arditi M. Bacterial lipopolysaccharide activates nuclear factor-kappaB through interleukin-1 signaling mediators in cultured human dermal endothelial cells and mononuclear phagocytes. J Biol Chem
1999;274:7611-4.
3. Yang RB, Mark MR, Gurney AL, Godowski PJ. Signaling events induced by lipopolysaccharide-activated toll-like receptor 2. J Immunol 1999;163:639-43. (RD1299)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

IRAK2 (C-term) antibody

Human Liver (formalin-fixed, paraffin-embedded) stained with IRAK2 antibody AP22850PU-N at 5 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit Purified Hu E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

IRAK2 (C-term) antibody

Human Testis (formalin-fixed, paraffin-embedded) stained with IRAK2 antibody AP22862PU-N at 5 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit Purified Hu E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

IRAK2 antibody

IRAK2 monoclonal antibody (M04), clone 1A6. Western Blot analysis of IRAK2 expression in NIH/3T3 ( Cat # L018V1 ). Mouse IgG1 1A6 Purified Hu, Ms, Rt E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

IRAK2 antibody

Western blot analysis of IRAK2 in HeLa (H) and K562 (K) whole cell lysate with IRAK2 antibody (C2) at 2 ug/mL. Rabbit IgG Purified Hu ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

IRAK2 (C-term) antibody

Western blot analysis of IRAK2 in K562 whole cell lysate with IRAK2 antibody at 1:500 dilution. Rabbit IgG Aff - Purified Hu E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

IRAK2 (C-term) antibody

Western blot analysis of IRAK2 in RAW264.7 whole cell lysate with mIRAK2 antibody at (A) 0.5, (B) 1, and (C) 2 ug/mL. Rabbit IgG Aff - Purified Hu, Ms E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

IRAK2 antibody

Western blot analysis of IRAK2 in A-20 whole cell lysate with IRAK2 antibody (C2) at (A) 0.5, (B) 1, and (C) 2 µg/ml. Rabbit IgG Aff - Purified Ms E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

IRAK2 antibody

Western blot analysis of extracts from 293 cells, using IRAK2 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

IRAK2 antibody

Predicted band size: 69 kDa . Positive control: Mouse lung tissue lysate. Recommended dilution: 1/1000-5000. (Gel: 10%SDS-PAGE Lysate: 30 ug Primary antibody: 1/1500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 20 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

IRAK2 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-IRAK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803648) Mouse IgG1 OTI4C1 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

IRAK2 antibody

E.coli lysate (left lane) and E.coli lysate expressing human recombinant protein fragment corresponding to amino acids 362-625 of human IRAK2 (NP_001561) were separated by SDS-PAGE and immunoblotted with anti-IRAK2. Mouse IgG1 OTI1B4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

IRAK2 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IRAK2 (RC218437, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IRAK2. Mouse IgG1 OTI2B7 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

IRAK2 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IRAK2 (RC218437, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IRAK2. Mouse IgG1 OTI1D4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

IRAK2 antibody

Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG1 6C7 Purified Hu E, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

IRAK2 antibody

Skin: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu P, WB
50 µg / €470.00
  Acris Antibodies GmbH

IRAK2 (N-term) antibody

Western blot analysis of anti-IRAK2 Pab in Jurkat cell lysate. IRAK1 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

IRAK2 (C-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

IRAK2 Control Peptide

IRAK2
50 µg / €160.00
  Acris Antibodies GmbH

IRAK2 Control Peptide

IRAK2
50 µg / €160.00
  Acris Antibodies GmbH

IRAK2 Control Peptide

IRAK2
50 µg / €160.00
  Acris Antibodies GmbH

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