IRAK3 antibody

Principal name

IRAK3 antibody

Alternative names for IRAK3 antibody

IRAK-3, IRAK-M, Interleukin-1 receptor-associated kinase 3, IL-1 receptor-associated kinase M

SwissProt ID

Q8K4B2 (Mouse), Q9Y616 (Human)

Gene ID

11213 (IRAK3), 73914 (Irak3)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit, Mouse, Goat

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)

Background of IRAK3 antibody

Interleukin-1 receptor (IL1R)-associated kinases (IRAKs) are important mediators in the signal transduction of Toll-like receptor (TLR) and IL1R family members, collectively referred to as TIRs. Binding of IL-1 to its cognate receptor results in the activation of the NFkappaB signaling pathway. A 100 kDa IL-1- dependent kinase termed IRAK-1 (for IL-1 receptor- associated kinase 1) coimmunoprecipitates with activated IL-1RI and is implicated as an upstream mediator of NFkappaB activation. A related Drosophila protein, Pelle, is a known upstream activator of Dorsal, the Drosophila homolog of NFkappaB. IRAK-2 is a proximal mediator of IL1, a component of the IL1R signaling complex, and is required for IL1R-induced NFkappaB activation. IRAK-4, like IRAK-1 and Pelle, has auto- and cross-phosphorylation kinase activity. IRAK-4 is strongly expressed in kidney and is also found in lung, testis, small intestine, breast, liver, and placenta. In contrast to the other IRAKs that are expressed in most cell types, IRAK-M is restricted to monocytic cells. IRAK-M mRNA transcripts are found predominantly in PBL and the monocytic cell lines U937 and THP-1.

General readings

del (2005) J. Immunol. 174 (5), 3032-3040

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

IRAK3 antibody

Western blot analysis of IRAK3 over-expressed 293 cell line, cotransfected with IRAK3 Validated Chimera RNAi ( Cat # H00011213-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with IRAK3 monoclonal antibody (M02) clone 1F6 (Cat # H00011213-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. Mouse IgG1 1F6 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

IRAK3 antibody

TA303364 (0.05µg/ml) staining of Rat Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detceted with chemiluminescence. Goat Aff - Purified Ms, Rt WB
20 µg / €200.00
  Acris Antibodies GmbH

IRAK3 antibody

TA303364 (0.05µg/ml) staining of Rat Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detceted with chemiluminescence. Goat Aff - Purified Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

IRAK3 antibody

Western blot analysis of IRAK-M in mouse spleen (M) and rat liver (R) tissue lysates with IRAK-M antibody at 1 ug/ml. Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

IRAK3 antibody

Sample (50 ug of whole cell lysate). A: mouse liver. 7.5% SDS PAGE. TA308809 diluted at 1:1000. Rabbit IgG Aff - Purified Hu, Ms ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

IRAK3 antibody

Western blot analysis of extracts of various cell lines, using IRAK3 antibody. Rabbit IgG Purified Hu, Ms
0.1 mg / €325.00
  OriGene Technologies, Inc.

IRAK3 antibody

WB Suggested Anti-IRAK3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

IRAK3 antibody

Western blot analysis on K562 cell lysate using IRAK3 Antibody Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

IRAK3 antibody

Immunohistochemistry (IHC) analyzes of IRAK-M antibody (Cat.-No.: AP01287PU-N) in paraffin-embedded human brain tissue. Rabbit Aff - Purified Hu E, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

IRAK3 antibody

Tonsil: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu, Ms, Rt P, WB
50 µg / €470.00
  Acris Antibodies GmbH

IRAK3 (N-term) antibody

Western blot analysis of anti-IRAK3 Pab in HL-60 cell lysate. IRAK3 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

IRAK3 Control Peptide

IRAK3
50 µg / €160.00
  Acris Antibodies GmbH

IRAK3 antibody

Western Blot analysis of IRAK3 expression in transfected 293T cell line (H00011213-T01) by IRAK3 MaxPab polyclonal antibody.

Lane 1: IRAK3 transfected lysate(65.56 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

IRAK3 antibody

IRAK3 Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

IRAK3 antibody

IRAK3 Rabbit Serum Hu E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

IRAK3 antibody

Western Blot analysis of IRAK3 expression in transfected 293T cell line (H00011213-T02) by IRAK3 MaxPab polyclonal antibody.

Lane 1: IRAK3 transfected lysate(67.80 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

IRAK3 antibody

IRAK3 monoclonal antibody (M01), clone 1A6 Western Blot analysis of IRAK3 expression in HeLa ( Cat # L013V1 ). Mouse IgG1 1A6 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

IRAK3 antibody

IRAK3 monoclonal antibody (M04), clone 1G11 Western Blot analysis of IRAK3 expression in HeLa ( Cat # L013V1 ). Mouse IgG1 1G11 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

IRAK3 antibody

IRAK3 Mouse IgG1 1F1 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

IRAK3 antibody

IRAK3 Mouse IgG1 1C8 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

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