IRF1 antibody

Principal name

IRF1 antibody

Alternative names for IRF1 antibody

Interferon regulatory factor 1, IRF-1

SwissProt ID

A0FIN4 (Pig), P10914 (Human), P15314 (Mouse), P23570 (Rat), Q3SZP0 (Bovin), Q90876 (Chick)

Gene ID

3659 (IRF1), 16362 (Irf1)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Por (Porcine), Rb (Rabbit), Rt (Rat), Chk (Chicken), GP (Guinea Pig), Sh (Sheep), Ye (Yeast), Eq (Equine)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P)

Background of IRF1 antibody

IRF1 is interferon regulatory factor 1, a member of the interferon regulatory transcription factor (IRF) family. IRF1 serves as an activator of interferons alpha and beta transcription, and in mouse it has been shown to be required for double-stranded RNA induction of these genes. IRF1 also functions as a transcription activator of genes induced by interferons alpha, beta, and gamma. Further, IRF1 has been shown to play roles in regulating apoptosis and tumor-suppressoion.IRF1 encodes interferon regulatory factor 1, a member of the interferon regulatory transcription factor (IRF) family. IRF1 serves as an activator of interferons alpha and beta transcription, and in mouse it has been shown to be required for double-stranded RNA induction of these genes. IRF1 also functions as a transcription activator of genes induced by interferons alpha, beta, and gamma. Further, IRF1 has been shown to play roles in regulating apoptosis and tumor-suppressoion.IRF1 encodes interferon regulatory factor 1, a member of the interferon regulatory transcription factor (IRF) family. IRF1 serves as an activator of interferons alpha and beta transcription, and in mouse it has been shown to be required for double-stranded RNA induction of these genes. IRF1 also functions as a transcription activator of genes induced by interferons alpha, beta, and gamma. Further, IRF1 has been shown to play roles in regulating apoptosis and tumor-suppressoion. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human IRF1 with an internal ID of P24052

General readings

Silva, L.K., et al. Eur. J. Hum. Genet. 18(11):1221-1227(2010)
Matsuzaki, S., et al. J. Immunol. 185(8):4863-4872(2010)
Antonios, D., et al. J. Immunol. 185(1):89-98(2010)
Lou, Y.J., et al. Zhonghua Yi Xue Yi Chuan Xue Za Zhi 27(3):255-258(2010)
Schuurhof, A., et al. Pediatr. Pulmonol. 45(6):608-613(2010)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

IRF1 antibody

Predicted band size: 37 kDa . Positive control: NIH/3T3 and Jurkat cell lysate. Recommended dilution: 1/500-2000. (Gel: 12%SDS-PAGE Lane1: NIH/3T3 cell lysate Lane2: Jurkat cell lysate Lysates: 30 ug per lane Primary antibody: 1/750 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

IRF1 antibody

WB Suggested Anti-IRF1 Antibody Titration: 0.2-1 ug/ml; Positive Control: HepG2 cell lysate Rabbit IgG Purified Can, Eq, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

IRF1 antibody

WB Suggested Anti-IRF1 Antibody; Titration: 1.0 ug/ml; Positive Control: Hela Whole Cell Rabbit IgG Purified Bov, Eq, GP, Hu, Rt, Sh WB
50 µg / €325.00
  OriGene Technologies, Inc.

IRF1 antibody

WB Suggested Anti-Irf1 Antibody Titration: 0.2-1 ug/ml; Positive Control: Mouse Muscle Rabbit IgG Purified Bov, Can, GP, Hu, Ms, Rb, Rt, Sh WB
50 µg / €325.00
  OriGene Technologies, Inc.

IRF1 antibody

WB Suggested Anti-Irf1 Antibody Titration: 0.2-1 ug/ml; Positive Control: Mouse Uterus Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Sh, Ye WB
50 µg / €325.00
  OriGene Technologies, Inc.

IRF1 antibody

ELISA: IRF1 Antibody Rabbit IgG Purified Hu E
0.1 mg / €325.00
  OriGene Technologies, Inc.

IRF1 (Center) antibody

Western blot analysis of IRF1 Antibody (Center) Cat.-No AP52233PU-N in MDA-MB231 cell line lysates (35ug/lane). This demonstrates the IRF1 antibody detected the IRF1 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

IRF1 (C-term) antibody

Western blot analysis of IRF1 Antibody (C-term) Cat.-No AP52234PU-N in MDA-MB231 cell line lysates (35ug/lane). This demonstrates the IRF1 antibody detected the IRF1 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

IRF1 antibody

IRF1 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

IRF1 antibody

Immunofluorescence of purified MaxPab antibody to IRF1 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Serum Hu E(detection), ICC/IF, WB
50 µl / €370.00
  Abnova Taiwan Corp.

IRF1 antibody

IRF1 Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

IRF1 antibody

Immunoprecipitation of IRF1 transfected lysate using anti-IRF1 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with IRF1 MaxPab mouse polyclonal antibody (B01) (H00003659-B01). Rabbit Serum Hu E(detection), IP
0.1 ml / €380.00
  Abnova Taiwan Corp.

IRF1 antibody

IRF1 monoclonal antibody (M01), clone 2E4 Western Blot analysis of IRF1 expression in COLO 320 HSR ( Cat # L020V1 ). Mouse IgG2a 2E4 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

IRF1 antibody

IRF1 Mouse IgG2a 1C1 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

IRF1 antibody

Human HepG2; WB Suggested Anti-IRF1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: HepG2 cell lysate; IRF1 antibody - N-terminal region (ARP31296_P050) in Human HepG2 cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Por, Rb, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

IRF1 (C-term) antibody

Mouse Muscle; WB Suggested Anti-Irf1 Antibody Titration: 0.2-1 ug/ml. Positive Control: Mouse Muscle; Irf1 antibody - C-terminal region (ARP36902_P050) in Mouse Muscle cells using Western Blot Rabbit Aff - Purified Ms WB
50 µg / €350.00
  AVIVA Systems Biology

IRF1 (Center) antibody

Mouse Uterus; WB Suggested Anti-Irf1 Antibody Titration: 0.2-1 ug/ml. Positive Control: Mouse Uterus; Irf1 antibody - middle region (ARP36903_P050) in Mouse Uterus cells using Western Blot Rabbit Aff - Purified Ms WB
50 µg / €350.00
  AVIVA Systems Biology

IRF1 antibody

Human HepG2; WB Suggested Anti-IRF1 Antibody Titration: 0.2-1 ug/ml. Positive Control: HepG2 cell lysate; IRF1 antibody - N-terminal region (ARP38128_P050) in Human HepG2 cells using Western Blot Rabbit Aff - Purified Bov, Can, Chk, Hu, Ms, Por, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

IRF1 antibody

ELISA: IRF1 Antibody [30340002] Rabbit Hu E
50 µg / €470.00
  Novus Biologicals Inc.

IRF1 antibody

Western Blot: IRF1 Antibody [NBP1-78761]-Whole cell lysate from NIH3T3 cells (M; 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded).  Antibodies: Affinity purified rabbit anti-IRF1 antibody  used for WB at 0.2 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate.  IRF1 was also immunoprecipitated by rabbit anti-IRF1 antibodies NBP1-78762 and NBP1-78763 which recognize downstream epitopes. Rabbit IgG Aff - Purified Ms IP, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

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