IRF5 antibody

Principal name

IRF5 antibody

Alternative names for IRF5 antibody

Interferon regulatory factor 5, IRF-5

SwissProt ID

P56477 (Mouse), Q13568 (Human), Q58DJ0 (Bovin)

Gene ID

3663 (IRF5), 27056 (Irf5)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Rt (Rat), Chk (Chicken), Por (Porcine), Ze (Zebrafish), African clawed frog, Eq (Equine), Rb (Rabbit)

Available hosts

Mouse, Rabbit, Goat

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Enzyme Immunoassay (E), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), ChIP assay (ChIP)

Background of IRF5 antibody

Interferon regulatory factors (IRFs) comprise a family of transcription factors that function within the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection. IRFs play an important role in pathogen defense, autoimmunity, lymphocyte development, cell growth, and susceptibility to transformation. The IRF family includes nine members: IRF-1, IRF-2, ISGF3?/p48, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7, and IRF-8/ICSBP. All IRF proteins share homology in their amino-termil D-binding domains. IRF family members regulate transcription through interactions with proteins that share similar D-binding motifs, such as IFN-stimulated response elements (ISRE), IFN consensus sequences (ICS), and IFN regulatory elements (IRF-E). IRF-5 is expressed in lymphoid tissues and peripheral blood lymphocytes and participates in the induction of type I interferon genes following viral infection. Activation of IRF-5 sigling is triggered by the toll-like receptor (TLR) pathway, including TLR7 and MyD88. Genetic variants of IRF-5 have been associated with disorders where the IFN pathway is abnormally activated, such as systemic lupus erythematosus .

General readings

Barnes B.J., et al (2001). J. Biol. Chem. 276:23382-23390
Schoenemeyer A., et al (2005). J. Biol. Chem. 280:17005-17012

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

IRF5 antibody

  IRF5 mouse monoclonal antibody, clone OTI1G7 (formerly 1G7)  
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-IRF5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA507252) Mouse IgG1 OTI1G7 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

IRF5 (176-240) antibody

Immunohistochemistry
Paraffin embedded sections of normal colon mucosa and colon adenocalcinoma tissue were incubated with anti-human IRF-5 antibody (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB). Mouse IgG1 10T1 Purified Hu, Ms E, F, ICC/IF, IP, P, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

IRF5 (176-240) antibody

Immunohistochemistry
Paraffin embedded sections of normal colon mucosa and colon adenocalcinoma tissue were incubated with anti-human IRF-5 antibody (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB). Mouse IgG1 10T1 Purified Hu, Ms E, F, ICC/IF, IP, P, WB
50 µl / €230.00
  Acris Antibodies GmbH

IRF5 (C-term) antibody

  Goat Polyclonal Antibody against IRF5  
TA302667 (0.1ug/ml) staining of lysate of cell line A549 (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Hu, Ms, Rt ChIP, E, IP, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

IRF5 antibody

  Rabbit polyclonal anti-IRF5 antibody  
Western blot analysis of extracts from HepG2 cells, using IRF5 Antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

IRF5 (N-term) antibody

  Rabbit polyclonal IRF5 Antibody (N-term)  
Western blot analysis of anti-IRF5 Antibody (N-term) (Cat.#TA324738) in Ramos cell line lysates (35ug/lane). IRF5 (arrow) was detected using the purified Pab. Rabbit Ig Purified Hu WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

IRF5 antibody

  IRF5 Rabbit Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using IRF5 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

IRF5 antibody

  Rabbit Polyclonal Anti-IRF5 Antibody  
WB Suggested Anti-IRF5 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: SH-SYSY cell lysate Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

IRF5 antibody

  Rabbit Polyclonal Anti-IRF5 Antibody  
WB Suggested Anti-IRF5 Antibody Titration: 2.5 ug/ml; ELISA Titer: 1: 312500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rb, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

IRF5 antibody

  Rabbit Polyclonal Anti-IRF5 Antibody  
WB Suggested Anti-IRF5 Antibody Titration: 0.15 ug/ml; ELISA Titer: 1:62500; Positive Control: Human Lymphnode Rabbit IgG Purified Ms, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

IRF5 antibody

  IRF5 mouse monoclonal antibody, clone OTI1A5 (formerly 1A5)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IRF5 (RC200458, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IRF5. Mouse IgG2a OTI1A5 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

IRF5 antibody

  IRF5 mouse monoclonal antibody, clone OTI1B8 (formerly 1B8)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IRF5 (RC200458, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IRF5. Mouse IgG1 OTI1B8 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

IRF5 antibody

Western Blot analysis: THP-1 cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-IRF5 Antibody Cat.-No AP33091PU (Clone 11F4A09). Proteins were visualized using an anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection. Mouse IgG2b 11F4A09 Aff - Purified Hu F, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

IRF5 antibody

Western Blot analysis: THP-1 cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-IRF5 Antibody Cat.-No AP33091PU (Clone 11F4A09). Proteins were visualized using an anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection. Mouse IgG2b 11F4A09 Aff - Purified Hu F, WB
25 µg / €260.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

IRF5 - human gene knockout kit via CRISPR

IRF5 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

IRF5 - human gene knockout kit via CRISPR

IRF5 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

IRF5 - human gene knockout kit via CRISPR

IRF5 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

IRF5 - human gene knockout kit via CRISPR

IRF5 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Irf5 - mouse gene knockout kit via CRISPR

Irf5 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Irf5 - mouse gene knockout kit via CRISPR

Irf5 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

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