IRS1 antibody

Principal name

IRS1 antibody

Alternative names for IRS1 antibody

Insulin receptor substrate 1, IRS-1

SwissProt ID

P35568 (Human), P35569 (Mouse), P35570 (Rat)

Gene ID

3667 (IRS1), 16367 (Irs1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Chk (Chicken), Mky (Monkey), Por (Porcine)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Enzyme Immunoassay (E), Dot blot (Dot), Immunoprecipitation (IP), ELISA (detection) (E(detection))

Background of IRS1 antibody

The insulin receptor substrate-1 (IRS-1), a major substrate of the insulin receptor, is phosphorylated in response to stimulation of cells by insulin, insulin-like growth factor 1 (IGF-1) and interleukin 4 (IL-4). IRS-1 is phosphorylated on serine, threonine and tyrosine residues in a variety of tissues. An insulin-sensitive serine/threonine kinase casein kinase II mediates a portion of the insulin-stimulated serine/threonine phosphorylation of overexpressed IRS-1 in vivo. Thr 502 is identified as the major casein kinase II-catalyzed phosphorylation site in rat IRS-1, and Ser 99 is an additional phosphorylation site catalyzed by casein kinase II. Thus, casein kinase II-catalyzed phosphorylation of IRS-1 may be a component of the intracellular insulin signaling cascade. IRS-1 contains three putative binding sites for 14-3-3 (Ser 270, Ser 374 and Ser 641) and the motif around Ser 270 is located in the phosphortyrosine binding domain of IRS-1, which is responsible for the interaction with the insulin receptor. The association of 14-3-3 with IRS-1 increases significantly upon treatment with okadaic acid, a potent serine/ threonine phosphatase inhibitor. Therefore, the association of 14-3-3 protein may play a role in the regulation of insulin sensitivity by interrupting the association between the insulin receptor and IRS-1.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

IRS1 antibody

Western blot analysis of extracts from NIH-3T3 cells, using IRS-1 (Ab-312) Antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

IRS1 antibody

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-IRS1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808550)(1:150) Mouse IgG1 OTI2B10 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

IRS1 antibody

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-IRS1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808552)(1:150) Mouse IgG2a OTI9E11 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

IRS1 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-IRS1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808553)(1:150) Mouse IgG1 OTI3G10 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

IRS1 (1223-1242) antibody

Human colon ca. stained with Anti-IRS-1, cat# AP15610PU Rabbit Purified Hu, Ms, Rt P
0.5 ml / €360.00
  Acris Antibodies GmbH

IRS1 (1223-1242) antibody

Human colon ca. stained with Anti-IRS-1, cat# AP15610PU Rabbit Purified Hu, Ms, Rt P
1 ml / €530.00
  Acris Antibodies GmbH

IRS1 (1223-1242) antibody

Human colon ca. stained with Anti-IRS-1, cat# AP15610PU Rabbit Purified Hu, Ms, Rt P
0.1 ml / €200.00
  Acris Antibodies GmbH

IRS1 antibody

Western blot analysis of IRS-1 in PC-3 cell lysate with IRS-1 antibody at (A) 1, (B) 2 and (C) 4 ug/mL. Rabbit IgG Aff - Purified Hu, Ms E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

IRS1 antibody

Western blot analysis of extracts from 293 cells, using IRS-1 (phospho-Ser312) antibody (Lanes 1 and 2). Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

IRS1 antibody

Western blot analysis of extracts from A431 cells, using IRS-1 (Ab-307) antibody (#TA312858, Line 1 and 2).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

IRS1 antibody

Western blot analysis of extracts from MCF7 cells treated with IGF-2, and COS7 cells treated with Insulin, using IRS-1 (Phospho-Ser1101) Antibody (#TA312866).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

IRS1 antibody

Western blot analysis of extracts from HepG2 cells, using IRS-1 (Phospho-Ser312) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

IRS1 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using IRS-1 (Phospho-Ser307) Antibody.The picture on the right is treated with the synthesized peptide. Rabbit IgG Purified E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

IRS1 antibody

Western blot analysis of extracts from K562 cells, using IRS-1 (Phospho-Ser307) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

IRS1 antibody

Western blot analysis of extracts from 293 cells and COLO205 cells, using IRS-1 (Phospho-Ser636) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

IRS1 pSer307 antibody

WB using Anti-IRS1 pS307 antibody shows detection of a band at ~180 kDa believed to represent phosphorylated IRS1 (arrowhead). Lane 1 shows staining of human 293 cell lysate. Lane 2 shows staining of 293 cell lysate prepared from cells serum-starved for 18 h followed by treatment with 5 µg/ml of anisomysin for 30 min. The primary antibody diluted to 1:250. IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX was used at 1:10000. Rabbit Aff - Purified Hu E
0.1 mg / €325.00
  OriGene Technologies, Inc.

IRS1 antibody

Predicted band size: 180 kDa . Positive control: 293 cells untreated or treated with insulin lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane1: 293 cells untreated with insulin lysate Lane2: 293 cells treated with insulin lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

IRS1 antibody

Predicted band size: 180 kDa . Positive control: 3T3 cells treated with Insulin lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane1: 3T3 cells untreated with Insulin lysate Lane2: 3T3 cells treated with Insulin lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

IRS1 antibody

Predicted band size: 180 kDa . Positive control: 293 cells untreated or treated with serum lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane1: 293 cells untreated with serum lysate Lane2: 293 cells treated with serum lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

IRS1 antibody

Western blot analysis of IRS-1 expression in NIH-3T3 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

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