IRS1 antibody

Principal name

IRS1 antibody

Alternative names for IRS1 antibody

Insulin receptor substrate 1, IRS-1

SwissProt ID

P35568 (Human), P35569 (Mouse), P35570 (Rat)

Gene ID

3667 (IRS1), 16367 (Irs1)

Available reactivities

Hu (Human), Rb (Rabbit), Chk (Chicken), Ms (Mouse), Rt (Rat), Bov (Bovine), Mky (Monkey), Can (Canine), Eq (Equine), Por (Porcine)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P), Western blot / Immunoblot (WB), Flow Cytometry (F), ELISA (detection) (E(detection)), Frozen Sections (C), Dot blot (Dot)

Background of IRS1 antibody

Insulin receptor substrate (IRS) molecules are key mediators in insulin signaling and play a central role in maintaining basic cellular functions such as growth, survival, and metabolism. They act as docking proteins between the insulin receptor and a complex network of intracellular signaling molecules containing Src homology 2 (SH2) domains. Four members (IRS-1, IRS-2, IRS-3, IRS-4) of this family have been identified that differ as to tissue distribution, subcellular localization, developmental expression, binding to the insulin receptor, and interaction with SH2 domain-containing proteins. Results from targeted disruption of the IRS genes in mice have provided important clues to the functional differences among these related molecules, suggesting they play different and specific roles in vivo. The available data are consistent with the notion that IRS-1 and IRS-2 are not functionally interchangeable in tissues that are responsible for glucose production (liver), glucose uptake (skeletal muscle and adipose tissue), and insulin production (pancreatic β cells). In fact, IRS-1 appears to have its major role in skeletal muscle whereas IRS-2 appears to regulate hepatic insulin action as well as pancreatic β cell development and survival. By contrast, IRS-3 and IRS-4 genes appear to play a redundant role in the IRS signaling system. Defects in muscle IRS-1 expression and function have been reported in insulin-resistant states such as obesity and type 2 diabetes.

General readings

J Biol Chem. 2004 Oct 29;279(44):45304-7. Epub 2004 Sep 10.
Protein kinase C Theta inhibits insulin signaling by phosphorylating IRS1 at Ser(1101).
Li Y, Soos TJ, Li X, Wu J, Degennaro M, Sun X, Littman DR, Birnbaum MJ, Polakiewicz RD.
Cell Signaling Technology, Inc., Beverly, Massachusetts 01915, USA.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

IRS1 antibody

Western blot analysis of extracts from NIH-3T3 cells, using IRS-1 (Ab-312) Antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

IRS1 antibody

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-IRS1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808550)(1:150) Mouse IgG1 OTI2B10 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

IRS1 antibody

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-IRS1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808552)(1:150) Mouse IgG2a OTI9E11 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

IRS1 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-IRS1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808553)(1:150) Mouse IgG1 OTI3G10 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

IRS1 (1223-1242) antibody

Human colon ca. stained with Anti-IRS-1, cat# AP15610PU Rabbit Purified Hu, Ms, Rt P
0.5 ml / €360.00
  Acris Antibodies GmbH

IRS1 (1223-1242) antibody

Human colon ca. stained with Anti-IRS-1, cat# AP15610PU Rabbit Purified Hu, Ms, Rt P
1 ml / €520.00
  Acris Antibodies GmbH

IRS1 (1223-1242) antibody

Human colon ca. stained with Anti-IRS-1, cat# AP15610PU Rabbit Purified Hu, Ms, Rt P
0.1 ml / €190.00
  Acris Antibodies GmbH

IRS1 antibody

Western blot analysis of IRS-1 in PC-3 cell lysate with IRS-1 antibody at (A) 1, (B) 2 and (C) 4 ug/mL. Rabbit IgG Aff - Purified Hu, Ms E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

IRS1 antibody

Western blot analysis of extracts from A431 cells, using IRS-1 (Ab-307) antibody (#TA312858, Line 1 and 2).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

IRS1 pSer307 antibody

WB using Anti-IRS1 pS307 antibody shows detection of a band at ~180 kDa believed to represent phosphorylated IRS1 (arrowhead). Lane 1 shows staining of human 293 cell lysate. Lane 2 shows staining of 293 cell lysate prepared from cells serum-starved for 18 h followed by treatment with 5 µg/ml of anisomysin for 30 min. The primary antibody diluted to 1:250. IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX was used at 1:10000. Rabbit Aff - Purified Hu E
0.1 mg / €325.00
  OriGene Technologies, Inc.

IRS1 antibody

Western blot analysis of IRS-1 expression in NIH-3T3 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

IRS1 antibody

IRS1 Rabbit IgG Purified Hu, Ms, Rt P
0.1 ml / €325.00
  OriGene Technologies, Inc.

IRS1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IRS1 (RC208765, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IRS1.(1:2000) Mouse IgG1 OTI1D10 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

IRS1 pSer616 antibody

Indirect ELISA: Serial dilution of the antibody starting with 5 µg/well was done to check for the affinity. 1 µg of coated antigen can be sensitively detected by anti-Human IRS pSer616 antibody used at 300 pg/well concentration. Mouse IgG1 HIR-B1 Aff - Purified Hu E, WB
0.1 mg / €410.00
  Acris Antibodies GmbH

IRS1 antibody

IRS1 Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €370.00
  Acris Antibodies GmbH

IRS1 pSer794 antibody

Immunohistochemistry (IHC) analysis of IRS-1 pSer794 antibody Cat.-No AP01831PU-N in paraffin-embedded human breast carcinoma at 1/100. Rabbit Aff - Purified Hu, Ms, Rt P
0.1 mg / €380.00
  Acris Antibodies GmbH

IRS1 pSer312 antibody

Figure 1. Western blot analysis of extract from 293 cells, using IRS-1 (Phospho-Ser312) antibody AP02428PU (Lane 1 and 2). Rabbit Aff - Purified Hu, Ms WB
0.1 ml / €360.00
  Acris Antibodies GmbH

IRS1 pSer312 antibody

Figure 1. Western blot analysis of extract from 293 cells, using IRS-1 (Phospho-Ser312) antibody AP02428PU (Lane 1 and 2). Rabbit Aff - Purified Hu, Ms WB
50 µl / €270.00
  Acris Antibodies GmbH

IRS1 pSer636 antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using IRS-1 antibody (AP02488PU-S; AP02488PU-N). Rabbit Aff - Purified Hu, Ms P, WB
0.1 ml / €320.00
  Acris Antibodies GmbH

IRS1 pSer636 antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using IRS-1 antibody (AP02488PU-S; AP02488PU-N). Rabbit Aff - Purified Hu, Ms P, WB
50 µl / €240.00
  Acris Antibodies GmbH

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